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The map label for this gene is cynR [H]

Identifier: 86747853

GI number: 86747853

Start: 817178

End: 818071

Strand: Reverse

Name: cynR [H]

Synonym: RPB_0727

Alternate gene names: 86747853

Gene position: 818071-817178 (Counterclockwise)

Preceding gene: 86747854

Following gene: 86747852

Centisome position: 15.34

GC content: 66.89

Gene sequence:

>894_bases
TTGATCGACAAGCTCGAGCTGCTGCTGGCGCTCGCCAAGGAACGCCATTTCGGACGGGCGGCGGAAGCCTGCGGCGTCAC
CCAGCCGACGATGTCGACCAGCATCAAACAGCTCGAGGAAATCCTCGGCGTGATGCTGGTGCAGCGCGGTTCGCGGTTCC
TCGGCTTCACGCCGGAAGGCGAACGGACGCTCGACTGGGCGCGCCGCATCGTCGGCGATACGCGAGCGATGCGCCAGGAC
ATCGGCGCCATGAAGGGCGAGCTGTCGGGGGAGCTCCGGATCGCCGCGATCCCCACCGTGCTGGGCATGGTGGCCTCGCT
CACCACGCCGTTCCGCGCGCGCTATCCGAACGTGCGCTTCAGCGTGCTGTCCTGCACCTCGTCGGAAGTGCTCGGTCTGC
TGGAGAATCTCGAGGCGGACGCCGGGCTCACCTATATCGAGAACGAACCGCTCGGCAAGGTGCACAGCATCCCGCTGTAT
CAGGAAAGCTACCGGCTGATCACCTCGCCCGACGGCATGTTCGGCGATCGCGATCAGGTGACCTGGAAGGAAGTCGGCCA
GGTGCCGCTGTGCCTGCTGACCCCGAACATGCAGAACCGCCGCATCATCGACCGCGCGCTGCGCGAGGCCGGCACCGAGG
CGCATCCGACCCTGACGTCGAATTCGATCATCGTGCTCTATACCCACGTCAAGACCGGGCGCTGGGCCAGCGTGATGCCC
GCCAAGCTCGCCGAAACGCTCGGTCTCGGCGACGGCGTCCGCACCATCCCGATCGTCGATCCGATGGTGTCCTACAGCAT
CGGCCTGGTGGTGCCGCAGCGCGAGCCCTACACGCCGCAGGTTGCGGCGCTGATGCAGACCGCGCGCGACATCGCGCCGA
CGCTGCAGAGCTGA

Upstream 100 bases:

>100_bases
CGCAAGCGAGCGGGAGAGGGGGCGGGCTGTGACTGCCTTGCCGAATGCCGCCTGATCGGGCAAGACTCGCGTCATATTTC
GCAAGTTTGACGGGGCCGGT

Downstream 100 bases:

>100_bases
TCAGCCTCTCCCGCTTCATTATCGTTTCCGTATCGCACCATTGGACAGCTTTATTGATTCTTTGAACAATTCTTATTCTC
ATCGGGCGAGCCACGACGAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLEEILGVMLVQRGSRFLGFTPEGERTLDWARRIVGDTRAMRQD
IGAMKGELSGELRIAAIPTVLGMVASLTTPFRARYPNVRFSVLSCTSSEVLGLLENLEADAGLTYIENEPLGKVHSIPLY
QESYRLITSPDGMFGDRDQVTWKEVGQVPLCLLTPNMQNRRIIDRALREAGTEAHPTLTSNSIIVLYTHVKTGRWASVMP
AKLAETLGLGDGVRTIPIVDPMVSYSIGLVVPQREPYTPQVAALMQTARDIAPTLQS

Sequences:

>Translated_297_residues
MIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLEEILGVMLVQRGSRFLGFTPEGERTLDWARRIVGDTRAMRQD
IGAMKGELSGELRIAAIPTVLGMVASLTTPFRARYPNVRFSVLSCTSSEVLGLLENLEADAGLTYIENEPLGKVHSIPLY
QESYRLITSPDGMFGDRDQVTWKEVGQVPLCLLTPNMQNRRIIDRALREAGTEAHPTLTSNSIIVLYTHVKTGRWASVMP
AKLAETLGLGDGVRTIPIVDPMVSYSIGLVVPQREPYTPQVAALMQTARDIAPTLQS
>Mature_297_residues
MIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLEEILGVMLVQRGSRFLGFTPEGERTLDWARRIVGDTRAMRQD
IGAMKGELSGELRIAAIPTVLGMVASLTTPFRARYPNVRFSVLSCTSSEVLGLLENLEADAGLTYIENEPLGKVHSIPLY
QESYRLITSPDGMFGDRDQVTWKEVGQVPLCLLTPNMQNRRIIDRALREAGTEAHPTLTSNSIIVLYTHVKTGRWASVMP
AKLAETLGLGDGVRTIPIVDPMVSYSIGLVVPQREPYTPQVAALMQTARDIAPTLQS

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790399, Length=290, Percent_Identity=28.6206896551724, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI157672245, Length=293, Percent_Identity=27.6450511945392, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788748, Length=290, Percent_Identity=25.8620689655172, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786401, Length=173, Percent_Identity=32.3699421965318, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI145693105, Length=270, Percent_Identity=23.3333333333333, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788296, Length=228, Percent_Identity=26.7543859649123, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787879, Length=199, Percent_Identity=28.1407035175879, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI2367136, Length=171, Percent_Identity=30.9941520467836, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787530, Length=244, Percent_Identity=23.7704918032787, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789506, Length=254, Percent_Identity=22.4409448818898, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081904, Length=172, Percent_Identity=25.5813953488372, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790208, Length=140, Percent_Identity=30.7142857142857, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32645; Mature: 32645

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLEEILGVMLVQRGSRFLGFTPEG
CCHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCC
ERTLDWARRIVGDTRAMRQDIGAMKGELSGELRIAAIPTVLGMVASLTTPFRARYPNVRF
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCHHHCCCCCEE
SVLSCTSSEVLGLLENLEADAGLTYIENEPLGKVHSIPLYQESYRLITSPDGMFGDRDQV
EEEECCHHHHHHHHHHCCCCCCCEEECCCCCCCEECCCCHHCCCEEEECCCCCCCCCCCC
TWKEVGQVPLCLLTPNMQNRRIIDRALREAGTEAHPTLTSNSIIVLYTHVKTGRWASVMP
CHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHH
AKLAETLGLGDGVRTIPIVDPMVSYSIGLVVPQREPYTPQVAALMQTARDIAPTLQS
HHHHHHHCCCCCCEEECCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLEEILGVMLVQRGSRFLGFTPEG
CCHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCC
ERTLDWARRIVGDTRAMRQDIGAMKGELSGELRIAAIPTVLGMVASLTTPFRARYPNVRF
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCHHHCCCCCEE
SVLSCTSSEVLGLLENLEADAGLTYIENEPLGKVHSIPLYQESYRLITSPDGMFGDRDQV
EEEECCHHHHHHHHHHCCCCCCCEEECCCCCCCEECCCCHHCCCEEEECCCCCCCCCCCC
TWKEVGQVPLCLLTPNMQNRRIIDRALREAGTEAHPTLTSNSIIVLYTHVKTGRWASVMP
CHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHH
AKLAETLGLGDGVRTIPIVDPMVSYSIGLVVPQREPYTPQVAALMQTARDIAPTLQS
HHHHHHHCCCCCCEEECCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]