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The map label for this gene is grpE

Identifier: 86747553

GI number: 86747553

Start: 470134

End: 470754

Strand: Direct

Name: grpE

Synonym: RPB_0427

Alternate gene names: 86747553

Gene position: 470134-470754 (Clockwise)

Preceding gene: 86747552

Following gene: 86747555

Centisome position: 8.82

GC content: 65.7

Gene sequence:

>621_bases
ATGACCGATTCCAACGGACCGAAGGACAACAACCAGGACCAGGCGCAGGCAGCCGCCGATCCGGTGGTTTCCAAGCCGTA
CATCATGCCGGACGACCCGGAAGACGGCGCCAACGAGGCGCTCATCAAGGAAGCCGCCGAAGCGCGCGACAAGATGCTGC
GCACGCTGGCGGAAATGGAGAATTTGCGCCGGCGGACCCAGAAAGAGGTCGCTGATGCACGGACCTATGGGGTCAGCGCG
TTCGCCCGCGACGTGCTCGAGATCGCCGACAATCTGCAGCGCGCGCTCGACGCCGTGCCGGCCGAAGCCCGCGCCAATGC
CGATGCCGGTCTCAAGGGGCTGATCGAGGGCGTCGAACTCACCGAGCGTTCACTGATCAACGCGCTGGAAAAGAACGGCG
TCCGGAAATTCGATCCGTCGGGAGAGAAGTTCGACCCCAATTTCCAGCAGGCGATGTACGAAGTTCCGGATCCGTCGGTG
CCCGCCGGCACAGTCGTGCAGGTGGTGCAGGCCGGCTTCATGATCGGCGAACGCGTGTTGCGCCCGGCGCTTGTCGGCGT
TGCGAAGGGCGGCGCGAAGCCCGCGCCCGCGGCGACCAACGGCACCGATACCACCGCCTGA

Upstream 100 bases:

>100_bases
TCATGCGGCGGCCGCCAAGCTTGATTTTCACGGCGCAAACACGATATCCCGGCCAACCCATTCCCGACCGATCCAGACTT
TTCTCTCAGAAGGTAGCGTG

Downstream 100 bases:

>100_bases
TCAGCGCGCCTTCAGCCCGTCGCGCACCTTTCGGAAACGCGGAAACGCGTCGGACCAGAGGTCGCGCGGCGAGCTGCCGA
TCACCCGCATCGCCACGCTG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTDSNGPKDNNQDQAQAAADPVVSKPYIMPDDPEDGANEALIKEAAEARDKMLRTLAEMENLRRRTQKEVADARTYGVSA
FARDVLEIADNLQRALDAVPAEARANADAGLKGLIEGVELTERSLINALEKNGVRKFDPSGEKFDPNFQQAMYEVPDPSV
PAGTVVQVVQAGFMIGERVLRPALVGVAKGGAKPAPAATNGTDTTA

Sequences:

>Translated_206_residues
MTDSNGPKDNNQDQAQAAADPVVSKPYIMPDDPEDGANEALIKEAAEARDKMLRTLAEMENLRRRTQKEVADARTYGVSA
FARDVLEIADNLQRALDAVPAEARANADAGLKGLIEGVELTERSLINALEKNGVRKFDPSGEKFDPNFQQAMYEVPDPSV
PAGTVVQVVQAGFMIGERVLRPALVGVAKGGAKPAPAATNGTDTTA
>Mature_205_residues
TDSNGPKDNNQDQAQAAADPVVSKPYIMPDDPEDGANEALIKEAAEARDKMLRTLAEMENLRRRTQKEVADARTYGVSAF
ARDVLEIADNLQRALDAVPAEARANADAGLKGLIEGVELTERSLINALEKNGVRKFDPSGEKFDPNFQQAMYEVPDPSVP
AGTVVQVVQAGFMIGERVLRPALVGVAKGGAKPAPAATNGTDTTA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=181, Percent_Identity=38.121546961326, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI40255109, Length=150, Percent_Identity=34, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1788967, Length=183, Percent_Identity=34.4262295081967, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17552458, Length=162, Percent_Identity=35.8024691358025, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324806, Length=151, Percent_Identity=41.0596026490066, Blast_Score=125, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24653432, Length=144, Percent_Identity=42.3611111111111, Blast_Score=133, Evalue=9e-32,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_RHOP2 (Q2J322)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484049.1
- ProteinModelPortal:   Q2J322
- SMR:   Q2J322
- STRING:   Q2J322
- GeneID:   3909983
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0427
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14141
- BioCyc:   RPAL316058:RPB_0427-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21945; Mature: 21814

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSNGPKDNNQDQAQAAADPVVSKPYIMPDDPEDGANEALIKEAAEARDKMLRTLAEME
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NLRRRTQKEVADARTYGVSAFARDVLEIADNLQRALDAVPAEARANADAGLKGLIEGVEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHH
TERSLINALEKNGVRKFDPSGEKFDPNFQQAMYEVPDPSVPAGTVVQVVQAGFMIGERVL
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RPALVGVAKGGAKPAPAATNGTDTTA
HHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDSNGPKDNNQDQAQAAADPVVSKPYIMPDDPEDGANEALIKEAAEARDKMLRTLAEME
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NLRRRTQKEVADARTYGVSAFARDVLEIADNLQRALDAVPAEARANADAGLKGLIEGVEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHH
TERSLINALEKNGVRKFDPSGEKFDPNFQQAMYEVPDPSVPAGTVVQVVQAGFMIGERVL
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RPALVGVAKGGAKPAPAATNGTDTTA
HHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA