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The map label for this gene is mltA [H]

Identifier: 86747527

GI number: 86747527

Start: 445419

End: 446939

Strand: Direct

Name: mltA [H]

Synonym: RPB_0401

Alternate gene names: 86747527

Gene position: 445419-446939 (Clockwise)

Preceding gene: 86747526

Following gene: 86747528

Centisome position: 8.35

GC content: 68.44

Gene sequence:

>1521_bases
GTGCTTGCCGCCGCCTTGCTGTTCGCGCCGGCTGCCGTCGAAGCCGGGCCGCGCCACCACCACTCCTCTGGCCATCATTC
CTCTGGCCATCACGCCAAGCCCCACCACGCCGCCGCACACAAGCGGCATCGGCTGCCGGCCGGGCCGATCGAAATCCCCG
GCGGGCAGTATGCCCCCGTGAGCTGGGACCAGGTCCCCGGCTGGGATTCCGACGATCAGCTCGCCGCCTTCAAGACGTTT
CAGGCCAGCTGCCGGCCGGTTCTGGCCCGGCGCAAGCCGATGCCGGATACCAAGGCGCTCGGGTCGTCGTTGGTCGCGCC
GTGCCGCGCGGCGCGTGTCGCCGAGATCTCCGACGCCGCTCAGGCCCGCCGCTTCTTCGAACGCCATTTCCTGCCGCTGC
AGATCTCACGGGTCGGCGAGGACGCAGGCTTCGTCACCGGCTATTACGAGCCGATCGTCGACGGATCGCGTGTGCAGACC
GACGTCTACAATGTGCCGGTGTATCGGCGGCCGTCCAACCTGTTCGTGCGCGGCTATTCGCAGGGCGCCGGCCTGCCCAA
TGGCGGGCCGGTGTATCGTAAGATCGGCCGGCGGAAGCTGGTGCCGTATTATGATCGCGCCGAGATCGAGGACGGCGCCA
TCGCCGGTCGCGGGCTGGAAATCTGCTGGCTGAAAAGCCAGACCGATCTCTTGTTCTCGCAGATTCAGGGCTCGGCCCGG
GTCAGGCTCGAGGACGGCAGCTTCGTGCGCATCAATTACGACGCCCACAACGGCTATCCCTATACGCCGGTCGGCCGCAT
CCTGATCGATCGCGGCATCATCCCGAAAGAGCAGATGTCGATGCAGCGCATCCGCGAATGGATGGACGCCAATCCGGACG
GCGCCAAGGAATTGCGGCGGGCCAACCGCTCCTTCGTGTTCTTCCGCGAGGTCAGGCTGAAAGACGACGACGAGGCGGTC
GGCGCGCAGGGCGTGCCGCTGACGCCGGGTCGCTCGATCGCGGTCGACAAGGGATTGCACGTCTACGGCACGCCGTTCTT
CATCACCGGCGAACTGCCGGTCGAGGCTCCCGAGGCGAAGACGCCGTTCCGACGGTTGATGATCGCGCAGGACACCGGCT
CGGCGATCATCGGCCCGGCGCGGGCGGATCTGTATTTCGGCGCCGGCGCGGAGGCGGGCGCGGTGGCTGGGCGGATGCGG
CATCCGATCCGCTTCGTGATGCTGCTGCCCAACAGCCTCGATCCGGTCGCGCGCGGCCGCACCATGCCGCTGCCCGAACC
GCGGCCATCGGCCAGGATCGCCAAGCAGTTTCCGCCGACGCCGGTCGCGAAAGACAAGCCGGTCGAAGCGGATGCCGCCA
AGCCGACGGCGACGCCCGCCACCAAGCCGGCTTCTCCGTTGGAAGGTTCGGCGCAGGCGGTTCCGACGCCACAGGCGCGG
CCCGATGCAGCGCCGGCCGCTTCACCGAAGCGAACGCACCGCCGGCCTCACCGGCATCGTCATCACCGACGCCATCGCTG
A

Upstream 100 bases:

>100_bases
GCCCGCGATATCAGCTCGCGCGATCCGAACTGGAAGCTGGTTGGCACCGGAAACGAGCTCTAGCGCGCGGGGCGCAACGC
GCTCTGCCTGGGCGCGCGGC

Downstream 100 bases:

>100_bases
CGACGATGAAGCGCGTCGCCCCACCGCTCGATCCACCCGCAGCCCCGCGCCGCAAGCGCAGCCTGAGCGAGGACGAGCGC
GTGCTGTGGGACAGCGTCGC

Product: membrane-bound lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase A [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MLAAALLFAPAAVEAGPRHHHSSGHHSSGHHAKPHHAAAHKRHRLPAGPIEIPGGQYAPVSWDQVPGWDSDDQLAAFKTF
QASCRPVLARRKPMPDTKALGSSLVAPCRAARVAEISDAAQARRFFERHFLPLQISRVGEDAGFVTGYYEPIVDGSRVQT
DVYNVPVYRRPSNLFVRGYSQGAGLPNGGPVYRKIGRRKLVPYYDRAEIEDGAIAGRGLEICWLKSQTDLLFSQIQGSAR
VRLEDGSFVRINYDAHNGYPYTPVGRILIDRGIIPKEQMSMQRIREWMDANPDGAKELRRANRSFVFFREVRLKDDDEAV
GAQGVPLTPGRSIAVDKGLHVYGTPFFITGELPVEAPEAKTPFRRLMIAQDTGSAIIGPARADLYFGAGAEAGAVAGRMR
HPIRFVMLLPNSLDPVARGRTMPLPEPRPSARIAKQFPPTPVAKDKPVEADAAKPTATPATKPASPLEGSAQAVPTPQAR
PDAAPAASPKRTHRRPHRHRHHRRHR

Sequences:

>Translated_506_residues
MLAAALLFAPAAVEAGPRHHHSSGHHSSGHHAKPHHAAAHKRHRLPAGPIEIPGGQYAPVSWDQVPGWDSDDQLAAFKTF
QASCRPVLARRKPMPDTKALGSSLVAPCRAARVAEISDAAQARRFFERHFLPLQISRVGEDAGFVTGYYEPIVDGSRVQT
DVYNVPVYRRPSNLFVRGYSQGAGLPNGGPVYRKIGRRKLVPYYDRAEIEDGAIAGRGLEICWLKSQTDLLFSQIQGSAR
VRLEDGSFVRINYDAHNGYPYTPVGRILIDRGIIPKEQMSMQRIREWMDANPDGAKELRRANRSFVFFREVRLKDDDEAV
GAQGVPLTPGRSIAVDKGLHVYGTPFFITGELPVEAPEAKTPFRRLMIAQDTGSAIIGPARADLYFGAGAEAGAVAGRMR
HPIRFVMLLPNSLDPVARGRTMPLPEPRPSARIAKQFPPTPVAKDKPVEADAAKPTATPATKPASPLEGSAQAVPTPQAR
PDAAPAASPKRTHRRPHRHRHHRRHR
>Mature_506_residues
MLAAALLFAPAAVEAGPRHHHSSGHHSSGHHAKPHHAAAHKRHRLPAGPIEIPGGQYAPVSWDQVPGWDSDDQLAAFKTF
QASCRPVLARRKPMPDTKALGSSLVAPCRAARVAEISDAAQARRFFERHFLPLQISRVGEDAGFVTGYYEPIVDGSRVQT
DVYNVPVYRRPSNLFVRGYSQGAGLPNGGPVYRKIGRRKLVPYYDRAEIEDGAIAGRGLEICWLKSQTDLLFSQIQGSAR
VRLEDGSFVRINYDAHNGYPYTPVGRILIDRGIIPKEQMSMQRIREWMDANPDGAKELRRANRSFVFFREVRLKDDDEAV
GAQGVPLTPGRSIAVDKGLHVYGTPFFITGELPVEAPEAKTPFRRLMIAQDTGSAIIGPARADLYFGAGAEAGAVAGRMR
HPIRFVMLLPNSLDPVARGRTMPLPEPRPSARIAKQFPPTPVAKDKPVEADAAKPTATPATKPASPLEGSAQAVPTPQAR
PDAAPAASPKRTHRRPHRHRHHRRHR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi [H]

COG id: COG2821

COG function: function code M; Membrane-bound lytic murein transglycosylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789179, Length=268, Percent_Identity=32.089552238806, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010611
- InterPro:   IPR014733
- InterPro:   IPR005300 [H]

Pfam domain/function: PF06725 3D; PF03562 MltA [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 55532; Mature: 55532

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAAALLFAPAAVEAGPRHHHSSGHHSSGHHAKPHHAAAHKRHRLPAGPIEIPGGQYAPV
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCC
SWDQVPGWDSDDQLAAFKTFQASCRPVLARRKPMPDTKALGSSLVAPCRAARVAEISDAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QARRFFERHFLPLQISRVGEDAGFVTGYYEPIVDGSRVQTDVYNVPVYRRPSNLFVRGYS
HHHHHHHHCCCCEEEECCCCCCCEEEEEHHHCCCCCEEEEEEECCCCEECCCCEEEEEEC
QGAGLPNGGPVYRKIGRRKLVPYYDRAEIEDGAIAGRGLEICWLKSQTDLLFSQIQGSAR
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCEE
VRLEDGSFVRINYDAHNGYPYTPVGRILIDRGIIPKEQMSMQRIREWMDANPDGAKELRR
EEECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
ANRSFVFFREVRLKDDDEAVGAQGVPLTPGRSIAVDKGLHVYGTPFFITGELPVEAPEAK
CCCCEEEEEEEECCCCCHHCCCCCCCCCCCCCEEECCCCEEECCCEEEECCCCCCCCCCC
TPFRRLMIAQDTGSAIIGPARADLYFGAGAEAGAVAGRMRHPIRFVMLLPNSLDPVARGR
CCHHHEEEEECCCCEEECCCCCCEEECCCCCHHHHHHHHCCCEEEEEECCCCCCHHHCCC
TMPLPEPRPSARIAKQFPPTPVAKDKPVEADAAKPTATPATKPASPLEGSAQAVPTPQAR
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDAAPAASPKRTHRRPHRHRHHRRHR
CCCCCCCCCHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MLAAALLFAPAAVEAGPRHHHSSGHHSSGHHAKPHHAAAHKRHRLPAGPIEIPGGQYAPV
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCC
SWDQVPGWDSDDQLAAFKTFQASCRPVLARRKPMPDTKALGSSLVAPCRAARVAEISDAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QARRFFERHFLPLQISRVGEDAGFVTGYYEPIVDGSRVQTDVYNVPVYRRPSNLFVRGYS
HHHHHHHHCCCCEEEECCCCCCCEEEEEHHHCCCCCEEEEEEECCCCEECCCCEEEEEEC
QGAGLPNGGPVYRKIGRRKLVPYYDRAEIEDGAIAGRGLEICWLKSQTDLLFSQIQGSAR
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCEE
VRLEDGSFVRINYDAHNGYPYTPVGRILIDRGIIPKEQMSMQRIREWMDANPDGAKELRR
EEECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
ANRSFVFFREVRLKDDDEAVGAQGVPLTPGRSIAVDKGLHVYGTPFFITGELPVEAPEAK
CCCCEEEEEEEECCCCCHHCCCCCCCCCCCCCEEECCCCEEECCCEEEECCCCCCCCCCC
TPFRRLMIAQDTGSAIIGPARADLYFGAGAEAGAVAGRMRHPIRFVMLLPNSLDPVARGR
CCHHHEEEEECCCCEEECCCCCCEEECCCCCHHHHHHHHCCCEEEEEECCCCCCHHHCCC
TMPLPEPRPSARIAKQFPPTPVAKDKPVEADAAKPTATPATKPASPLEGSAQAVPTPQAR
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDAAPAASPKRTHRRPHRHRHHRRHR
CCCCCCCCCHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]