Click here to switch to the map view.

The map label for this gene is amtB [H]

Identifier: 86747499

GI number: 86747499

Start: 413316

End: 414758

Strand: Direct

Name: amtB [H]

Synonym: RPB_0373

Alternate gene names: 86747499

Gene position: 413316-414758 (Clockwise)

Preceding gene: 86747498

Following gene: 86747500

Centisome position: 7.75

GC content: 67.43

Gene sequence:

>1443_bases
ATGACGTTCAAACGTCCCTACGGCGCGGGACTGGCCGCCCTGGCAGCCGGCCTGTTCGCCGCGACTGCCGCCTACGCCGA
GCCCACGGTCAACAAGGGCGACAACGCCTGGATGATGACCTCCACGGTTCTGGTCGTGCTGATGACCATCCCCGGCCTGG
CGCTGTTCTACGGCGGCCTGGTGCGCTCCAAGAACATGCTCTCGGTGCTGGCGCAGGTGTTCTACACCTTCTCGATCGTG
ATCGTGATCTGGGCGGTCTACGGCTACAGCCTGGCTTTCACCGGCGGCTCCGATTTCATCGGCGGCTTCTCCAAGGCATT
CCTCGCCGGCGTCACCCCCGACTCCAAGGTCGCGTCGTTCAGCGTCGACGCCAACGTCTCGGAGCTGATCTACATCTGCT
TCCAGATGACCTTCGCGGCGATCACGCCGGCGCTGATCGTCGGCGCCTTCGCCGAACGCACCCGCTTTGCCGCGGTCGCG
CTGTTCGTGCCGCTGTGGGTGACGCTGATCTACTTCCCGATGGCCCACATGGTCTGGTACTGGCCGGGTCCGGATTTGAT
CACCGACGCCGCCAAGGCGCTCGCCGCAGCCACTGACGATGCCGCCAAGGCGGCCGCGCAGGCCAAGCTCGACGAGATCA
ATGCGGACGCGGGCTTCATCTTCAAGAAGGGCGCGCTCGACTTCGCGGGCGGCACCGTGGTGCACATCAACGCCGGCATC
GCCGGCCTGGTCGGCGCCCTGCTGATCGGCAAGCGCACCGGCTACGGCAAGGAGCTGATGGCTCCGCACTCGCTGACCAT
GACCATGATCGGCGCGGCGCTGCTCTGGGTCGGCTGGTTCGGCTTCAACGTCGGCTCGAATCTCGAAGCCACCGGCGTCG
CCGGTCTCGCCATGACCAACACCTTCGTCGCCACGGCGGCGGCGGCGATGGGCTGGATGTTTGCGGAGTGGATCTTCAAG
GGTCACCCCTCGCTGCTCGGCGCGTTGTCCGGCGCAGTCGCGGGTCTGGTCGCGGTCACCCCCGCGGCGGGCTTCGCCGG
CCCGATGGGCGCGATCGTGCTCGGCCTGGTCGCCGGCGTGGTCTGCCTGTTCTTCTGCACCGTGGTGAAGAACGCGCTCG
GCTATGACGACTCGCTCGACGTGTTCGGCGTCCACGGCGTCGGCGGCATCATCGGCGCGCTCGGCACCGGCATCCTGGTG
AACCCGGCGCTCGGCGGCACCGGCGTCTACGACTACGTCGCCGGCAAGGTCGCCGACTACGACTTCGGCACCCAGATGAT
CGCCCAGTGCTGGGGCGTCGGCACCACCATCCTGCTGTCCGGCATCGGCTCGGCGATCCTGTTCAAGGTCGTCGACGTCA
TCGTCGGCCTGCGTGCCCCGGTCGAAGTCGAGCGCGAAGGCCTCGACCTCGTCGAGCACACCGAGCGCGCCTACAACATG
TGA

Upstream 100 bases:

>100_bases
GGCCTCGACCACGCCGTGCGCATCCGCACCGGCGAAGCCGACGCAGCAGCTCTCTAAGATTTCCCGCTCACCCAACTTTC
TTCCACTCAGGAGACGACAA

Downstream 100 bases:

>100_bases
GCTCTCCCGAGGGGCGCGGCGTTCTTCCTCCAGGAAGGCCGCGCCCAGGACTACGGTTCGGGCAGATACCCGGCACTGTC
CGAACCGTCGAGGGGTCCTG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTFKRPYGAGLAALAAGLFAATAAYAEPTVNKGDNAWMMTSTVLVVLMTIPGLALFYGGLVRSKNMLSVLAQVFYTFSIV
IVIWAVYGYSLAFTGGSDFIGGFSKAFLAGVTPDSKVASFSVDANVSELIYICFQMTFAAITPALIVGAFAERTRFAAVA
LFVPLWVTLIYFPMAHMVWYWPGPDLITDAAKALAAATDDAAKAAAQAKLDEINADAGFIFKKGALDFAGGTVVHINAGI
AGLVGALLIGKRTGYGKELMAPHSLTMTMIGAALLWVGWFGFNVGSNLEATGVAGLAMTNTFVATAAAAMGWMFAEWIFK
GHPSLLGALSGAVAGLVAVTPAAGFAGPMGAIVLGLVAGVVCLFFCTVVKNALGYDDSLDVFGVHGVGGIIGALGTGILV
NPALGGTGVYDYVAGKVADYDFGTQMIAQCWGVGTTILLSGIGSAILFKVVDVIVGLRAPVEVEREGLDLVEHTERAYNM

Sequences:

>Translated_480_residues
MTFKRPYGAGLAALAAGLFAATAAYAEPTVNKGDNAWMMTSTVLVVLMTIPGLALFYGGLVRSKNMLSVLAQVFYTFSIV
IVIWAVYGYSLAFTGGSDFIGGFSKAFLAGVTPDSKVASFSVDANVSELIYICFQMTFAAITPALIVGAFAERTRFAAVA
LFVPLWVTLIYFPMAHMVWYWPGPDLITDAAKALAAATDDAAKAAAQAKLDEINADAGFIFKKGALDFAGGTVVHINAGI
AGLVGALLIGKRTGYGKELMAPHSLTMTMIGAALLWVGWFGFNVGSNLEATGVAGLAMTNTFVATAAAAMGWMFAEWIFK
GHPSLLGALSGAVAGLVAVTPAAGFAGPMGAIVLGLVAGVVCLFFCTVVKNALGYDDSLDVFGVHGVGGIIGALGTGILV
NPALGGTGVYDYVAGKVADYDFGTQMIAQCWGVGTTILLSGIGSAILFKVVDVIVGLRAPVEVEREGLDLVEHTERAYNM
>Mature_479_residues
TFKRPYGAGLAALAAGLFAATAAYAEPTVNKGDNAWMMTSTVLVVLMTIPGLALFYGGLVRSKNMLSVLAQVFYTFSIVI
VIWAVYGYSLAFTGGSDFIGGFSKAFLAGVTPDSKVASFSVDANVSELIYICFQMTFAAITPALIVGAFAERTRFAAVAL
FVPLWVTLIYFPMAHMVWYWPGPDLITDAAKALAAATDDAAKAAAQAKLDEINADAGFIFKKGALDFAGGTVVHINAGIA
GLVGALLIGKRTGYGKELMAPHSLTMTMIGAALLWVGWFGFNVGSNLEATGVAGLAMTNTFVATAAAAMGWMFAEWIFKG
HPSLLGALSGAVAGLVAVTPAAGFAGPMGAIVLGLVAGVVCLFFCTVVKNALGYDDSLDVFGVHGVGGIIGALGTGILVN
PALGGTGVYDYVAGKVADYDFGTQMIAQCWGVGTTILLSGIGSAILFKVVDVIVGLRAPVEVEREGLDLVEHTERAYNM

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=472, Percent_Identity=48.5169491525424, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17550182, Length=472, Percent_Identity=27.3305084745763, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17550180, Length=463, Percent_Identity=26.9978401727862, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=478, Percent_Identity=24.4769874476987, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI32564042, Length=472, Percent_Identity=22.6694915254237, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324187, Length=459, Percent_Identity=33.1154684095861, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6325396, Length=464, Percent_Identity=29.5258620689655, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321559, Length=464, Percent_Identity=29.5258620689655, Blast_Score=166, Evalue=9e-42,
Organism=Drosophila melanogaster, GI161078314, Length=463, Percent_Identity=23.5421166306695, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49754; Mature: 49622

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKRPYGAGLAALAAGLFAATAAYAEPTVNKGDNAWMMTSTVLVVLMTIPGLALFYGGL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VRSKNMLSVLAQVFYTFSIVIVIWAVYGYSLAFTGGSDFIGGFSKAFLAGVTPDSKVASF
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEE
SVDANVSELIYICFQMTFAAITPALIVGAFAERTRFAAVALFVPLWVTLIYFPMAHMVWY
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
WPGPDLITDAAKALAAATDDAAKAAAQAKLDEINADAGFIFKKGALDFAGGTVVHINAGI
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCH
AGLVGALLIGKRTGYGKELMAPHSLTMTMIGAALLWVGWFGFNVGSNLEATGVAGLAMTN
HHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
TFVATAAAAMGWMFAEWIFKGHPSLLGALSGAVAGLVAVTPAAGFAGPMGAIVLGLVAGV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VCLFFCTVVKNALGYDDSLDVFGVHGVGGIIGALGTGILVNPALGGTGVYDYVAGKVADY
HHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCC
DFGTQMIAQCWGVGTTILLSGIGSAILFKVVDVIVGLRAPVEVEREGLDLVEHTERAYNM
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TFKRPYGAGLAALAAGLFAATAAYAEPTVNKGDNAWMMTSTVLVVLMTIPGLALFYGGL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VRSKNMLSVLAQVFYTFSIVIVIWAVYGYSLAFTGGSDFIGGFSKAFLAGVTPDSKVASF
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEE
SVDANVSELIYICFQMTFAAITPALIVGAFAERTRFAAVALFVPLWVTLIYFPMAHMVWY
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
WPGPDLITDAAKALAAATDDAAKAAAQAKLDEINADAGFIFKKGALDFAGGTVVHINAGI
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCH
AGLVGALLIGKRTGYGKELMAPHSLTMTMIGAALLWVGWFGFNVGSNLEATGVAGLAMTN
HHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
TFVATAAAAMGWMFAEWIFKGHPSLLGALSGAVAGLVAVTPAAGFAGPMGAIVLGLVAGV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VCLFFCTVVKNALGYDDSLDVFGVHGVGGIIGALGTGILVNPALGGTGVYDYVAGKVADY
HHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCC
DFGTQMIAQCWGVGTTILLSGIGSAILFKVVDVIVGLRAPVEVEREGLDLVEHTERAYNM
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]