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The map label for this gene is leuC [H]

Identifier: 86747474

GI number: 86747474

Start: 391132

End: 392544

Strand: Reverse

Name: leuC [H]

Synonym: RPB_0348

Alternate gene names: 86747474

Gene position: 392544-391132 (Counterclockwise)

Preceding gene: 86747475

Following gene: 86747469

Centisome position: 7.36

GC content: 66.88

Gene sequence:

>1413_bases
ATGTCCGCTGCAAAACCCACCACGCTGTACGACAAGATCTGGAACGACCATCTGGTGCACGAGGCCGAGGACGGCACCTG
CCTGCTGTATATCGACCGTCATCTGGTGCACGAAGTGACCAGCCCGCAGGCCTTCGAAGGGCTGCGCACCGCCGGCCGCA
AGGTCCATGCGCCGGAGAAGACGCTGGCGGTGGTCGATCACAACGTGCCGACCACCGATCGCTCCAAGCCTAATCCGGAT
CCGGAGAGCGCCGAGCAGATCGCCGCTCTGGCCGAGAACGCCCGCGACTTCGGCGTCACCTACTACAACGAGTTCGACAA
GCGGCAGGGCGTGGTCCACGTCATCGGCCCGGAGCAGGGCTTCACGCTGCCCGGCACCACCATCGTCTGCGGCGACAGCC
ACACCTCGACGCATGGCGCGTTCGGCGCGCTGGCGCACGGCATCGGCACCTCCGAGGTCGAGCACGTCCTCGCCACCCAG
ACGCTGATCCAGAAGAAGGCCAAGAACATGCGCGTGACCGTCGACGGCGATCTGCCGGACGGCGTCACCGCCAAGGACAT
CATCCTGGCCATCATCGGCGAGATCGGCACCGCGGGTGGCACCGGCTACGTGCTGGAATACGCCGGCGATGCGATCCGCG
CGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATCGAAGGCGGCGCCCGCGCCGGCCTGATCGCGCCCGAT
GAGAAGGCCTATGCGTACTTGAAGGGCCGCCCGATGGCGCCGACCGGCGCCCATTGGGACGCCGCGATGCGCTATTGGGA
CACGCTGCGTTCCGACGAGGGCGCGCATTTCGACCACGAACTCCGGCTCGATGCCGCAGCGCTGCCGCCGATCGTGACCT
GGGGCACCTCGCCCGAAGACGTCATCTCGGTCACCGGAAAGGTGCCGAACCCGGCCGACATCGCCGACGAGGCCAAGCGG
CTCTCCAAAGAGCGCGCGCTGGCCTATATGGGGCTGACGCCGGGGACGAAGATCACCGACATCAAGATCGACCGGATGTT
CATCGGTTCCTGCACCAACGGCCGGATCGAGGATCTGCGCGCCGCCGCCAAGGTCGCCGAGGGCAAGACCGTCAACGCCA
ATGTCAACGCCATCATCGTGCCGGGCTCCGGGCTGGTGAAGGAACAGGCCGAGGCCGAGGGGCTGGACAAGATCTTCGTC
AAGGCCGGCTTCGAATGGCGTGAGCCGGGCTGCTCGATGTGCCTCGCCATGAACCCCGACAAGCTCGCCCCGGAAGAGCG
CTGCGCCTCGACCTCGAACCGCAATTTCGAAGGCCGTCAGGGCTTCAAGGGCCGCACCCATCTGGTGTCGCCTGCGATGG
CGGCGGCGGCGGCGATCGCCGGCCACTTCGTCGACATCCGCGACTGGCGCTGA

Upstream 100 bases:

>100_bases
CGCTCGCGGCCTGAGCGCAAACGCTTTCGCGCGGTTGTCGCGCTGATGCGACGCACGCTTCCCAAACTCCGCTTCAAATT
CCGGTTCGAGGACTGACACG

Downstream 100 bases:

>100_bases
GCAACGCAATCATTGCCGGGCTCGACCCGGCAACCGCTCTTCAGAAGAGCGATGGACCGCCGAATCAAGTCCGGCGGTGA
CGGTCGGAGCGATGATGGGA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSAAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEKTLAVVDHNVPTTDRSKPNPD
PESAEQIAALAENARDFGVTYYNEFDKRQGVVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQ
TLIQKKAKNMRVTVDGDLPDGVTAKDIILAIIGEIGTAGGTGYVLEYAGDAIRALSMEGRMTVCNMSIEGGARAGLIAPD
EKAYAYLKGRPMAPTGAHWDAAMRYWDTLRSDEGAHFDHELRLDAAALPPIVTWGTSPEDVISVTGKVPNPADIADEAKR
LSKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLRAAAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFV
KAGFEWREPGCSMCLAMNPDKLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWR

Sequences:

>Translated_470_residues
MSAAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEKTLAVVDHNVPTTDRSKPNPD
PESAEQIAALAENARDFGVTYYNEFDKRQGVVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQ
TLIQKKAKNMRVTVDGDLPDGVTAKDIILAIIGEIGTAGGTGYVLEYAGDAIRALSMEGRMTVCNMSIEGGARAGLIAPD
EKAYAYLKGRPMAPTGAHWDAAMRYWDTLRSDEGAHFDHELRLDAAALPPIVTWGTSPEDVISVTGKVPNPADIADEAKR
LSKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLRAAAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFV
KAGFEWREPGCSMCLAMNPDKLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWR
>Mature_469_residues
SAAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEKTLAVVDHNVPTTDRSKPNPDP
ESAEQIAALAENARDFGVTYYNEFDKRQGVVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMRVTVDGDLPDGVTAKDIILAIIGEIGTAGGTGYVLEYAGDAIRALSMEGRMTVCNMSIEGGARAGLIAPDE
KAYAYLKGRPMAPTGAHWDAAMRYWDTLRSDEGAHFDHELRLDAAALPPIVTWGTSPEDVISVTGKVPNPADIADEAKRL
SKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLRAAAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFVK
AGFEWREPGCSMCLAMNPDKLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=476, Percent_Identity=26.6806722689076, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=63.9308855291577, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI87081781, Length=340, Percent_Identity=25.5882352941176, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=476, Percent_Identity=27.9411764705882, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=387, Percent_Identity=28.6821705426357, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=29.9674267100977, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=383, Percent_Identity=27.9373368146214, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=64.755838641189, Blast_Score=629, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320440, Length=425, Percent_Identity=25.4117647058824, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=481, Percent_Identity=25.987525987526, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323335, Length=360, Percent_Identity=27.7777777777778, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571643, Length=477, Percent_Identity=25.9958071278826, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI281365315, Length=476, Percent_Identity=24.7899159663866, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17864292, Length=476, Percent_Identity=24.7899159663866, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=25.3926701570681, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24645686, Length=378, Percent_Identity=25.6613756613757, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17137564, Length=373, Percent_Identity=26.0053619302949, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50545; Mature: 50413

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEK
CCCCCCCHHHHHHCCCCCEEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCC
TLAVVDHNVPTTDRSKPNPDPESAEQIAALAENARDFGVTYYNEFDKRQGVVHVIGPEQG
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHCCCHHHHHHHHHCCEEEEECCCCC
FTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRVTVDGDLPD
CCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
GVTAKDIILAIIGEIGTAGGTGYVLEYAGDAIRALSMEGRMTVCNMSIEGGARAGLIAPD
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCC
EKAYAYLKGRPMAPTGAHWDAAMRYWDTLRSDEGAHFDHELRLDAAALPPIVTWGTSPED
CCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHCCCCEEECCCCHHH
VISVTGKVPNPADIADEAKRLSKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLR
HEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEEEECCCCCHHHHHH
AAAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFVKAGFEWREPGCSMCLAMNPD
HHHHHHCCCEEECCCCEEEECCCCCHHHHHHHCCHHEEHHHCCCCCCCCCCCEEEEECCC
KLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWR
CCCCHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
SAAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEK
CCCCCCHHHHHHCCCCCEEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCC
TLAVVDHNVPTTDRSKPNPDPESAEQIAALAENARDFGVTYYNEFDKRQGVVHVIGPEQG
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHCCCHHHHHHHHHCCEEEEECCCCC
FTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRVTVDGDLPD
CCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
GVTAKDIILAIIGEIGTAGGTGYVLEYAGDAIRALSMEGRMTVCNMSIEGGARAGLIAPD
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCC
EKAYAYLKGRPMAPTGAHWDAAMRYWDTLRSDEGAHFDHELRLDAAALPPIVTWGTSPED
CCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHCCCCEEECCCCHHH
VISVTGKVPNPADIADEAKRLSKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLR
HEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEEEECCCCCHHHHHH
AAAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFVKAGFEWREPGCSMCLAMNPD
HHHHHHCCCEEECCCCEEEECCCCCHHHHHHHCCHHEEHHHCCCCCCCCCCCEEEEECCC
KLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWR
CCCCHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA