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The map label for this gene is emrB [H]

Identifier: 86747334

GI number: 86747334

Start: 234162

End: 235745

Strand: Direct

Name: emrB [H]

Synonym: RPB_0208

Alternate gene names: 86747334

Gene position: 234162-235745 (Clockwise)

Preceding gene: 86747333

Following gene: 86747335

Centisome position: 4.39

GC content: 66.1

Gene sequence:

>1584_bases
ATGTCGACAGCGCACATCGTTGAAAGCGGCCCGGTCTCGACCGGCGGCATCGGTCCGCGGCCGCTGTTCGCGGTCGGCGC
GGTGCTGCTGGGCTCGTTCCTGTCGAATTTCGACAGCCGGCTGTTCGCGATCGCGCTGCCGGATCTGCGGGGCGCGCAGT
CGCTGAGCTTCGATCAGGGCGCCTGGCTGAGCACCGCCGCCACCGCGTCGCAGATCTTCATTGCGCCGGCGGTCGCCTGG
ATGGCGACTGCGTTCGGCCTGCGCCGTGTGCTCGGCCTTCCCAGTCTGGCCTATGCGGTCGTGTCGCTGCTGATCCCGCT
GGTGCACAGCTATGAGCTGATGATGGCGCTGAGCGTCGTCCACGGTCTTCTGCTCGGCATCTTCGTTCCGGCGACGCTGC
TGATCATCTTTCGCAATCTGCCGTTGCGCTGGTGGCTTCCGGCGATTTCGATCTACGCGATCCGCGTCGGCTTCAGCCTG
AATTTCGGCATCTCGGTGGTCGGGTTCTATGTCGATCAGATCGGCTGGCAATGGCTGTATTGGCAGGACGCGCTGATCGC
GCCGCTGCTCGGCCTGTTCGTCTATCTCGGGACGCCGGCCGAACCGGTGAATGCGCCGCTGGTGAAGCAGGCCGATTGGG
GCGGCATGCTGCTGCTGGGCTCCGGCATGGCGCTGATCTTTGCAGGGCTGGATCAGGGCAACCGGCTGGACTGGCTGGGT
TCGGGAACAGTGGTGGCTTGCCTCGGCTGCGGCGCCGCGCTGGTCGCAGGCTTCTTCGTCAATGAGCGCGTCGTCGCCCA
GCCCTGGGCGCATGCCAATGTGCTGTTCTCGCGCAATATCGGCTTCGCGCTGCTGATCATCCTGCTGTTCTCGCTGACCA
GTCTGTCGAACTCGCTGCTCGCGCCGGATTTCCTGGCGAGCGTTGCGGGATTGCGGCCGGAGCAATCGGGGCAATTGTTT
CTGGTCTATGCGGTGCTGCCGATGCTGCTGTGGGTGCCGCTGTCGGTCTGGCTGATCCGCCGGATCGATGTCCGGCTGGT
GCTGATCGCCGGGCTGGCGGCGTTCTGTGCGGCCGGCCTGCTCGGCACCCGGCTCACCCACGCCTGGGCGCTCGGCGATT
TCGTCCCGATGGTGCTGCTGCAATCCTTCGGCCAGGCTTTCACGCTACTGCCGATCGTGATCATCACGCTGTCCAATTCC
GATCCGCAGCGCGCCACAGCGTTCGCGGCCTATATTCAGGTCGCCCGGCTCGGTGGCGCCGAACTCAGCGTGTCGCTGAT
GACGACATGGCTGAGGGTGCGCGAACAATCGTATTCCAACCTGCTGGGGCTGCATGTCGCCAAGGGCGATCCGCTCGTCA
GCGGCGTCCTGTCCGACCTCACTCGGCATTTCGACGGCGCCGGCTCGGGCGAGGCGCAGTCGCGGGCGATCACGACACTG
GCGCAGATCGTCCAGCGCGAGGCGAATACGCTGTCGTTCATCGATGGGTTCTGGCTCGCGGCCTGGTTCGGCGTCGCCGC
GCTGCTGTGTGCGGCCTGCATCCGCGCCGCGCCGCCCGGACCATTCACCCCGAAAGGCGGTTGA

Upstream 100 bases:

>100_bases
CGGATCCAGTTCGCGCCCGGCCAGCCGCTGCTGGAGCGGTTGCTGCCGGGCATGTCGGTCACCACCCGAATCCATGCCGA
TGACGGTCCGGTCGGAGATC

Downstream 100 bases:

>100_bases
GCCACCTGCCGCTCTCACCACCAGAGGAGGTTTCATGTCGAAAGGTTGTACGCATATCGCGGGAATTCGCGACGTCACCC
CCAGCGCACTCGGTTGCGAG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MSTAHIVESGPVSTGGIGPRPLFAVGAVLLGSFLSNFDSRLFAIALPDLRGAQSLSFDQGAWLSTAATASQIFIAPAVAW
MATAFGLRRVLGLPSLAYAVVSLLIPLVHSYELMMALSVVHGLLLGIFVPATLLIIFRNLPLRWWLPAISIYAIRVGFSL
NFGISVVGFYVDQIGWQWLYWQDALIAPLLGLFVYLGTPAEPVNAPLVKQADWGGMLLLGSGMALIFAGLDQGNRLDWLG
SGTVVACLGCGAALVAGFFVNERVVAQPWAHANVLFSRNIGFALLIILLFSLTSLSNSLLAPDFLASVAGLRPEQSGQLF
LVYAVLPMLLWVPLSVWLIRRIDVRLVLIAGLAAFCAAGLLGTRLTHAWALGDFVPMVLLQSFGQAFTLLPIVIITLSNS
DPQRATAFAAYIQVARLGGAELSVSLMTTWLRVREQSYSNLLGLHVAKGDPLVSGVLSDLTRHFDGAGSGEAQSRAITTL
AQIVQREANTLSFIDGFWLAAWFGVAALLCAACIRAAPPGPFTPKGG

Sequences:

>Translated_527_residues
MSTAHIVESGPVSTGGIGPRPLFAVGAVLLGSFLSNFDSRLFAIALPDLRGAQSLSFDQGAWLSTAATASQIFIAPAVAW
MATAFGLRRVLGLPSLAYAVVSLLIPLVHSYELMMALSVVHGLLLGIFVPATLLIIFRNLPLRWWLPAISIYAIRVGFSL
NFGISVVGFYVDQIGWQWLYWQDALIAPLLGLFVYLGTPAEPVNAPLVKQADWGGMLLLGSGMALIFAGLDQGNRLDWLG
SGTVVACLGCGAALVAGFFVNERVVAQPWAHANVLFSRNIGFALLIILLFSLTSLSNSLLAPDFLASVAGLRPEQSGQLF
LVYAVLPMLLWVPLSVWLIRRIDVRLVLIAGLAAFCAAGLLGTRLTHAWALGDFVPMVLLQSFGQAFTLLPIVIITLSNS
DPQRATAFAAYIQVARLGGAELSVSLMTTWLRVREQSYSNLLGLHVAKGDPLVSGVLSDLTRHFDGAGSGEAQSRAITTL
AQIVQREANTLSFIDGFWLAAWFGVAALLCAACIRAAPPGPFTPKGG
>Mature_526_residues
STAHIVESGPVSTGGIGPRPLFAVGAVLLGSFLSNFDSRLFAIALPDLRGAQSLSFDQGAWLSTAATASQIFIAPAVAWM
ATAFGLRRVLGLPSLAYAVVSLLIPLVHSYELMMALSVVHGLLLGIFVPATLLIIFRNLPLRWWLPAISIYAIRVGFSLN
FGISVVGFYVDQIGWQWLYWQDALIAPLLGLFVYLGTPAEPVNAPLVKQADWGGMLLLGSGMALIFAGLDQGNRLDWLGS
GTVVACLGCGAALVAGFFVNERVVAQPWAHANVLFSRNIGFALLIILLFSLTSLSNSLLAPDFLASVAGLRPEQSGQLFL
VYAVLPMLLWVPLSVWLIRRIDVRLVLIAGLAAFCAAGLLGTRLTHAWALGDFVPMVLLQSFGQAFTLLPIVIITLSNSD
PQRATAFAAYIQVARLGGAELSVSLMTTWLRVREQSYSNLLGLHVAKGDPLVSGVLSDLTRHFDGAGSGEAQSRAITTLA
QIVQREANTLSFIDGFWLAAWFGVAALLCAACIRAAPPGPFTPKGG

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=502, Percent_Identity=21.9123505976096, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788710, Length=511, Percent_Identity=22.3091976516634, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56168; Mature: 56037

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAHIVESGPVSTGGIGPRPLFAVGAVLLGSFLSNFDSRLFAIALPDLRGAQSLSFDQG
CCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCC
AWLSTAATASQIFIAPAVAWMATAFGLRRVLGLPSLAYAVVSLLIPLVHSYELMMALSVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HGLLLGIFVPATLLIIFRNLPLRWWLPAISIYAIRVGFSLNFGISVVGFYVDQIGWQWLY
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCEECCCHHHHHHHHHHHCCEEEE
WQDALIAPLLGLFVYLGTPAEPVNAPLVKQADWGGMLLLGSGMALIFAGLDQGNRLDWLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCEEECC
SGTVVACLGCGAALVAGFFVNERVVAQPWAHANVLFSRNIGFALLIILLFSLTSLSNSLL
CCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCC
APDFLASVAGLRPEQSGQLFLVYAVLPMLLWVPLSVWLIRRIDVRLVLIAGLAAFCAAGL
CHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTRLTHAWALGDFVPMVLLQSFGQAFTLLPIVIITLSNSDPQRATAFAAYIQVARLGGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCC
ELSVSLMTTWLRVREQSYSNLLGLHVAKGDPLVSGVLSDLTRHFDGAGSGEAQSRAITTL
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AQIVQREANTLSFIDGFWLAAWFGVAALLCAACIRAAPPGPFTPKGG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STAHIVESGPVSTGGIGPRPLFAVGAVLLGSFLSNFDSRLFAIALPDLRGAQSLSFDQG
CCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCC
AWLSTAATASQIFIAPAVAWMATAFGLRRVLGLPSLAYAVVSLLIPLVHSYELMMALSVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HGLLLGIFVPATLLIIFRNLPLRWWLPAISIYAIRVGFSLNFGISVVGFYVDQIGWQWLY
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCEECCCHHHHHHHHHHHCCEEEE
WQDALIAPLLGLFVYLGTPAEPVNAPLVKQADWGGMLLLGSGMALIFAGLDQGNRLDWLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCEEECC
SGTVVACLGCGAALVAGFFVNERVVAQPWAHANVLFSRNIGFALLIILLFSLTSLSNSLL
CCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCC
APDFLASVAGLRPEQSGQLFLVYAVLPMLLWVPLSVWLIRRIDVRLVLIAGLAAFCAAGL
CHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTRLTHAWALGDFVPMVLLQSFGQAFTLLPIVIITLSNSDPQRATAFAAYIQVARLGGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCC
ELSVSLMTTWLRVREQSYSNLLGLHVAKGDPLVSGVLSDLTRHFDGAGSGEAQSRAITTL
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AQIVQREANTLSFIDGFWLAAWFGVAALLCAACIRAAPPGPFTPKGG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]