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The map label for this gene is braE [H]

Identifier: 86747270

GI number: 86747270

Start: 156383

End: 157318

Strand: Direct

Name: braE [H]

Synonym: RPB_0143

Alternate gene names: 86747270

Gene position: 156383-157318 (Clockwise)

Preceding gene: 86747269

Following gene: 86747271

Centisome position: 2.93

GC content: 67.31

Gene sequence:

>936_bases
ATGAGCCGCAGCAATATCGCCCTTCTGGTCCTGATCGCCTGCGTCGCGGCTCTGCCGCTGTTCGGCGGCGCCTATGCGCT
GCGGCTCGGCACCATCGCCTGCATGTATGCGGTGCTGGCGCTGTCGTGGAACGTGGTCGGCGGCTTCGCCGGCTATCCGT
CCTTCGCCACCGCGGCGTTCTTCGGCTTCGGCGCCTATTGCGGCGGCGTGCTGATGGGCAAGGGCTTCCCGCTGTGGGCG
GCGGCGGCGGTCGCCGGATTGCTGGCGCTGGTCTCCGCGGTGGCGCTCGGCGCGGTGCTGCTGCGGCTGCGCGGGCATTA
TTTCGCGATCGCCAGCCTGTCGCTCGCCGAGGTGCTGCGCGAGGTCGTCAACAACGCCACCGACCTCACCGGCGGCGGCA
TGGGGCTGAACATCCCGCTCGCCTCCGGCGCCGGCGTGATGGCGGAGGCGACGTTCTTCTTCTACGCGATGTGGGGCGTG
ATGCTGATCACCGCGCTGATGGTGATCGTGGTGGCGAATTCCAAGCTCGGCTTCGCGCTGAACTGCATCCGCCAGAACGA
GACCGCCTCCAGCATGGTCGGCCTCAACACCACGCTGGCCAAGAGCATCGCCTTCGGCCTGTCGGCCTGCTTCGTCAGCG
TCGCCGGCGGCATCTACGCCGCCTGGGTGCACTACATCGATCCGTCCGATGTGTTCGACATCCTGTATTCGGTCAAGCCT
ATCGTGATGGCGCTGATGGGCGGACTCGGCTCGCCGCTCGGCGTCGCCTGTGGCGCCTTCATGTATCTCGGCCTCGAGGA
AGTGGTGTGGCGCAACTACACCCACATCCACACCGGCGTGCTCGGCGTGCTGATCGTGATGCTGCTGCTGTTCCTGCCGC
ACGGCCTGAGCTCGCTGCGGTTCGACCGGCTGCGGCGGAGGACGAAGCATGTCTGA

Upstream 100 bases:

>100_bases
CGTTCTTCGGCCCGGCGCACGCCACCACGCTGTCGTTCCTGCTGTTGATCATCTTCCTGATCCTGCGCCCGCAGGGCCTG
GTCGGCCGAAAGGGCTTCGA

Downstream 100 bases:

>100_bases
GCTGCTGCAACTCACCGGCGTCTCCCGGCACTTCTCCGGCCTGCAGGCGCTGCGCGACGTCTCGCTGTCGGTGTCGGGCG
GCGAGGTGCTCGGCCTTATC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSRSNIALLVLIACVAALPLFGGAYALRLGTIACMYAVLALSWNVVGGFAGYPSFATAAFFGFGAYCGGVLMGKGFPLWA
AAAVAGLLALVSAVALGAVLLRLRGHYFAIASLSLAEVLREVVNNATDLTGGGMGLNIPLASGAGVMAEATFFFYAMWGV
MLITALMVIVVANSKLGFALNCIRQNETASSMVGLNTTLAKSIAFGLSACFVSVAGGIYAAWVHYIDPSDVFDILYSVKP
IVMALMGGLGSPLGVACGAFMYLGLEEVVWRNYTHIHTGVLGVLIVMLLLFLPHGLSSLRFDRLRRRTKHV

Sequences:

>Translated_311_residues
MSRSNIALLVLIACVAALPLFGGAYALRLGTIACMYAVLALSWNVVGGFAGYPSFATAAFFGFGAYCGGVLMGKGFPLWA
AAAVAGLLALVSAVALGAVLLRLRGHYFAIASLSLAEVLREVVNNATDLTGGGMGLNIPLASGAGVMAEATFFFYAMWGV
MLITALMVIVVANSKLGFALNCIRQNETASSMVGLNTTLAKSIAFGLSACFVSVAGGIYAAWVHYIDPSDVFDILYSVKP
IVMALMGGLGSPLGVACGAFMYLGLEEVVWRNYTHIHTGVLGVLIVMLLLFLPHGLSSLRFDRLRRRTKHV
>Mature_310_residues
SRSNIALLVLIACVAALPLFGGAYALRLGTIACMYAVLALSWNVVGGFAGYPSFATAAFFGFGAYCGGVLMGKGFPLWAA
AAVAGLLALVSAVALGAVLLRLRGHYFAIASLSLAEVLREVVNNATDLTGGGMGLNIPLASGAGVMAEATFFFYAMWGVM
LITALMVIVVANSKLGFALNCIRQNETASSMVGLNTTLAKSIAFGLSACFVSVAGGIYAAWVHYIDPSDVFDILYSVKPI
VMALMGGLGSPLGVACGAFMYLGLEEVVWRNYTHIHTGVLGVLIVMLLLFLPHGLSSLRFDRLRRRTKHV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=308, Percent_Identity=27.9220779220779, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 32647; Mature: 32516

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSNIALLVLIACVAALPLFGGAYALRLGTIACMYAVLALSWNVVGGFAGYPSFATAAF
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
FGFGAYCGGVLMGKGFPLWAAAAVAGLLALVSAVALGAVLLRLRGHYFAIASLSLAEVLR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EVVNNATDLTGGGMGLNIPLASGAGVMAEATFFFYAMWGVMLITALMVIVVANSKLGFAL
HHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
NCIRQNETASSMVGLNTTLAKSIAFGLSACFVSVAGGIYAAWVHYIDPSDVFDILYSVKP
HHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IVMALMGGLGSPLGVACGAFMYLGLEEVVWRNYTHIHTGVLGVLIVMLLLFLPHGLSSLR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
FDRLRRRTKHV
HHHHHHHHCCC
>Mature Secondary Structure 
SRSNIALLVLIACVAALPLFGGAYALRLGTIACMYAVLALSWNVVGGFAGYPSFATAAF
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH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HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]