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The map label for this gene is mepA [H]

Identifier: 86747178

GI number: 86747178

Start: 54035

End: 54991

Strand: Direct

Name: mepA [H]

Synonym: RPB_0051

Alternate gene names: 86747178

Gene position: 54035-54991 (Clockwise)

Preceding gene: 86747176

Following gene: 86747184

Centisome position: 1.01

GC content: 70.32

Gene sequence:

>957_bases
ATGCGTCGCCGATCGATTCCCGCCAGATGGCTGTTGGCCGCCGCGCTGCTCGGCGCCTCGGTGCCGTCTGCGTGGGCGCA
GGACAAGGGCACGGTCGATCCGAAGCCGCTGCCGCCGCTCGCCAACCCCAACGATCCCGAACTCGCCGCGCGCGAACTGT
TCGGCCGCCGGTATCTGCCCGCCGCGCTGCCGCCGCGGCCGATCGGGTTCTACGCCCGCGGCTGCCTTGCCGGCGCCGAG
GCGCTGCCGATCACCGGCAAGACCTGGCAGGTGATGCGGCTGTCGCGCAATCGGTTCTACGCCTATCCGGCGATGGTCGA
TCTGGTGGCGCGGCTGTCGCGGAAGGCGCACAGCGACGCCGGCTGGCCGGGCATTCTGGTCGGCGACATGTCGCAGCCGC
GCGGCGGGCCGATGCTGACCGGCCACGCCAGCCATCAGGTCGGGCTCGACGCCGACATCTGGCTGACGCCGATGCCGAAC
CGGCAGCTGTCGCGCAACGAGCGCGAGGAGATGTCGGCGGTGATGATGGTGCGCCGCGACCGGCTCGACATCGATCCCGC
GGCCTGGACGCCGACGCATTGGAAAGTGATCCGCGCCGCCGCGCAGGAGCCTCAAGTCGAGCGCATCTTCGCCAACGCCG
CGATCAAGAAGGCGCTGTGCCGCGAGGCCAAGGGCGACCGCGGCTGGCTCAACAAGGTGCGGCCGATGTACGGCCATGAT
TACCACTTCCACATCCGCATCAAATGCCCGCCCGGTCTCGCCGGCTGCGAGGCGCAACCCGAAACTCCGCAAGGCGAGGG
CTGCGCGCCCGCCGACCTCGCCTACTGGTTCTCCGACGCGGTGCTGCATCCCAAGCCGCCGAAAGTGCCGCCGAAGCCGA
AGCGCCAGCTCACCCTCGCCGAACTGCCGGCGGAATGCCGCGCCGTGCTGAACGCGCCCGACAAGAAGATCGACTAG

Upstream 100 bases:

>100_bases
GCATTAACATCGAGACCTTCGGCCGCGGGATGGCTCGCGATCCCGCCCGATCGCCAGCCCCCCGATTTCGCCAGCCTCCT
GGGTCGGCTATAAGGCGGCG

Downstream 100 bases:

>100_bases
CAGGCGAGGCCGCCGACGATCATGGCTTGCGCACGAACACCGCGGTGGCGTCGGGTGAGCGTGCCGGCGGCGTCATGTCG
GTCCAGAAACGGCCGTTGAT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRRRSIPARWLLAAALLGASVPSAWAQDKGTVDPKPLPPLANPNDPELAARELFGRRYLPAALPPRPIGFYARGCLAGAE
ALPITGKTWQVMRLSRNRFYAYPAMVDLVARLSRKAHSDAGWPGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPMPN
RQLSRNEREEMSAVMMVRRDRLDIDPAAWTPTHWKVIRAAAQEPQVERIFANAAIKKALCREAKGDRGWLNKVRPMYGHD
YHFHIRIKCPPGLAGCEAQPETPQGEGCAPADLAYWFSDAVLHPKPPKVPPKPKRQLTLAELPAECRAVLNAPDKKID

Sequences:

>Translated_318_residues
MRRRSIPARWLLAAALLGASVPSAWAQDKGTVDPKPLPPLANPNDPELAARELFGRRYLPAALPPRPIGFYARGCLAGAE
ALPITGKTWQVMRLSRNRFYAYPAMVDLVARLSRKAHSDAGWPGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPMPN
RQLSRNEREEMSAVMMVRRDRLDIDPAAWTPTHWKVIRAAAQEPQVERIFANAAIKKALCREAKGDRGWLNKVRPMYGHD
YHFHIRIKCPPGLAGCEAQPETPQGEGCAPADLAYWFSDAVLHPKPPKVPPKPKRQLTLAELPAECRAVLNAPDKKID
>Mature_318_residues
MRRRSIPARWLLAAALLGASVPSAWAQDKGTVDPKPLPPLANPNDPELAARELFGRRYLPAALPPRPIGFYARGCLAGAE
ALPITGKTWQVMRLSRNRFYAYPAMVDLVARLSRKAHSDAGWPGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPMPN
RQLSRNEREEMSAVMMVRRDRLDIDPAAWTPTHWKVIRAAAQEPQVERIFANAAIKKALCREAKGDRGWLNKVRPMYGHD
YHFHIRIKCPPGLAGCEAQPETPQGEGCAPADLAYWFSDAVLHPKPPKVPPKPKRQLTLAELPAECRAVLNAPDKKID

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=244, Percent_Identity=40.5737704918033, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 35215; Mature: 35215

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRSIPARWLLAAALLGASVPSAWAQDKGTVDPKPLPPLANPNDPELAARELFGRRYLP
CCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
AALPPRPIGFYARGCLAGAEALPITGKTWQVMRLSRNRFYAYPAMVDLVARLSRKAHSDA
CCCCCCCCHHHHHHHHCCCCCCCCCCCCHHEEEECCCCEEEHHHHHHHHHHHHHHHCCCC
GWPGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPMPNRQLSRNEREEMSAVMMVRRD
CCCEEEEECCCCCCCCCEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
RLDIDPAAWTPTHWKVIRAAAQEPQVERIFANAAIKKALCREAKGDRGWLNKVRPMYGHD
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHCCCC
YHFHIRIKCPPGLAGCEAQPETPQGEGCAPADLAYWFSDAVLHPKPPKVPPKPKRQLTLA
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEHH
ELPAECRAVLNAPDKKID
HCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRRRSIPARWLLAAALLGASVPSAWAQDKGTVDPKPLPPLANPNDPELAARELFGRRYLP
CCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
AALPPRPIGFYARGCLAGAEALPITGKTWQVMRLSRNRFYAYPAMVDLVARLSRKAHSDA
CCCCCCCCHHHHHHHHCCCCCCCCCCCCHHEEEECCCCEEEHHHHHHHHHHHHHHHCCCC
GWPGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPMPNRQLSRNEREEMSAVMMVRRD
CCCEEEEECCCCCCCCCEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
RLDIDPAAWTPTHWKVIRAAAQEPQVERIFANAAIKKALCREAKGDRGWLNKVRPMYGHD
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHCCCC
YHFHIRIKCPPGLAGCEAQPETPQGEGCAPADLAYWFSDAVLHPKPPKVPPKPKRQLTLA
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEHH
ELPAECRAVLNAPDKKID
HCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]