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The map label for this gene is pheS [H]

Identifier: 86747172

GI number: 86747172

Start: 44142

End: 45266

Strand: Direct

Name: pheS [H]

Synonym: RPB_0045

Alternate gene names: 86747172

Gene position: 44142-45266 (Clockwise)

Preceding gene: 86747171

Following gene: 86747173

Centisome position: 0.83

GC content: 64.89

Gene sequence:

>1125_bases
ATGACGGACGAGAGGCCGGTTCTGCATCCGAGAAGGATCGCCATGACCGACCTTTCGACTCTGCAATCTCAAATCCTCTC
CGACATCGCCGCGGCCTCCGACGAGGCCGCGCTGGAAGCGGTGCGCGTCGCCGCGCTCGGCAAGAAGGGCTCCGTCTCGG
CGCTGCTCGCCACGCTCGGCAAGATGGACCCCGAGCAGCGCAAGACCGAGGGCGCGGCGATCAATCTCGCCAAGGATGCG
GTGACGCAGGCGCTGGCCGCGCGGCGCGACGTGCTGAAGGCCGCCGCGCTCGACGCCAAGCTCGCCGCCGAGACCATCGA
CGTCACCCTGCCGCTGCGTGAACCGCTCGCCGAAGCCGGCCGGATTCATCCGCTCAGCCAGGTCTGGGACGAACTCACCG
CGATCTTCGCCGACATGGGTTTCGCCATCGCCGAAGGTCCGGACATCGAGACCGACGACTACAACTTCACCAAGCTGAAC
TTCCCCGAGGGCCATCCGGCCCGCGAGATGCACGACACCTTCTACTTCAACCCGAAGGACGACGGCTCGCGCCTGCTGCT
GCGCACCCACACCTCGCCGGTGCAGGTCCGCACCATGCTGAGCCAGAAGCCGCCGATCCGCGTGATCTGTCCGGGCCGCA
CCTATCGCAGCGACTCTGACCAGACCCACACGCCGATGTTCCATCAGGTCGAGGGTCTCGTCATCGACAAGGGCAGCCAC
CTCGGTCATCTGAAGTGGATCCTGCACGAATTCTGCAAGGCGTTCTTCGAGGTCGACAACGTCAATATGCGGTTCCGGCC
GTCGTTCTTCCCGTTCACCGAGCCGTCGCTGGAAGTCGACATCCAGTGCCGCCGCGGCAAGGACGAGATCCGCTTCGGCG
AGGGCGAGGACTGGCTGGAGATTCTCGGCTGCGGCATGGTGCACCCCAACGTGCTCACCGCCTGCGGCCTCGATCCCGAC
GTCTATCAGGGCTTCGCCTGGGGCATGGGCATCGACCGCATCGCGATGCTGAAATACGGCATGAGCGATCTGCGGCAATT
GTTCGAAGCCGATTCGCGCTGGCTGAATCATTACGGCTTCAAGCCGCTGGATATCCCGACGCTGGCGGGCGGATTGAGTT
CGTGA

Upstream 100 bases:

>100_bases
GTGCACGCAGTGAATTCGTCATGGCCGGGCTCGTCCCGGCCATCCACGTCTTTTCTGTTGGCCACGCCGCAAGGCGTGGA
TGCCCGGGACAAGCCCGGGC

Downstream 100 bases:

>100_bases
GCTTGGTACTCGCAGGCGCTGTCTGTGTTGTGCTGGACGTGGAGGCTGCGACAAGCGTCGCCCTCACCCCAGCCCTCTCC
CGCAAGCGGGAGAGGGGGCA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MTDERPVLHPRRIAMTDLSTLQSQILSDIAAASDEAALEAVRVAALGKKGSVSALLATLGKMDPEQRKTEGAAINLAKDA
VTQALAARRDVLKAAALDAKLAAETIDVTLPLREPLAEAGRIHPLSQVWDELTAIFADMGFAIAEGPDIETDDYNFTKLN
FPEGHPAREMHDTFYFNPKDDGSRLLLRTHTSPVQVRTMLSQKPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSH
LGHLKWILHEFCKAFFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLEILGCGMVHPNVLTACGLDPD
VYQGFAWGMGIDRIAMLKYGMSDLRQLFEADSRWLNHYGFKPLDIPTLAGGLSS

Sequences:

>Translated_374_residues
MTDERPVLHPRRIAMTDLSTLQSQILSDIAAASDEAALEAVRVAALGKKGSVSALLATLGKMDPEQRKTEGAAINLAKDA
VTQALAARRDVLKAAALDAKLAAETIDVTLPLREPLAEAGRIHPLSQVWDELTAIFADMGFAIAEGPDIETDDYNFTKLN
FPEGHPAREMHDTFYFNPKDDGSRLLLRTHTSPVQVRTMLSQKPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSH
LGHLKWILHEFCKAFFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLEILGCGMVHPNVLTACGLDPD
VYQGFAWGMGIDRIAMLKYGMSDLRQLFEADSRWLNHYGFKPLDIPTLAGGLSS
>Mature_373_residues
TDERPVLHPRRIAMTDLSTLQSQILSDIAAASDEAALEAVRVAALGKKGSVSALLATLGKMDPEQRKTEGAAINLAKDAV
TQALAARRDVLKAAALDAKLAAETIDVTLPLREPLAEAGRIHPLSQVWDELTAIFADMGFAIAEGPDIETDDYNFTKLNF
PEGHPAREMHDTFYFNPKDDGSRLLLRTHTSPVQVRTMLSQKPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSHL
GHLKWILHEFCKAFFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLEILGCGMVHPNVLTACGLDPDV
YQGFAWGMGIDRIAMLKYGMSDLRQLFEADSRWLNHYGFKPLDIPTLAGGLSS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=276, Percent_Identity=32.9710144927536, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1788007, Length=344, Percent_Identity=52.9069767441861, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32563657, Length=271, Percent_Identity=32.1033210332103, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=271, Percent_Identity=32.1033210332103, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32566635, Length=264, Percent_Identity=31.0606060606061, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321087, Length=272, Percent_Identity=31.6176470588235, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=108, Percent_Identity=34.2592592592593, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18858079, Length=271, Percent_Identity=30.6273062730627, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17137424, Length=291, Percent_Identity=24.3986254295533, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 41573; Mature: 41442

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDERPVLHPRRIAMTDLSTLQSQILSDIAAASDEAALEAVRVAALGKKGSVSALLATLG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
KMDPEQRKTEGAAINLAKDAVTQALAARRDVLKAAALDAKLAAETIDVTLPLREPLAEAG
CCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHC
RIHPLSQVWDELTAIFADMGFAIAEGPDIETDDYNFTKLNFPEGHPAREMHDTFYFNPKD
CCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCCCCCHHHHHHCEEECCCC
DGSRLLLRTHTSPVQVRTMLSQKPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSH
CCCEEEEEECCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCEEEECCCC
LGHLKWILHEFCKAFFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLE
CHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHH
ILGCGMVHPNVLTACGLDPDVYQGFAWGMGIDRIAMLKYGMSDLRQLFEADSRWLNHYGF
HHHCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCC
KPLDIPTLAGGLSS
CCCCCHHHCCCCCC
>Mature Secondary Structure 
TDERPVLHPRRIAMTDLSTLQSQILSDIAAASDEAALEAVRVAALGKKGSVSALLATLG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
KMDPEQRKTEGAAINLAKDAVTQALAARRDVLKAAALDAKLAAETIDVTLPLREPLAEAG
CCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHC
RIHPLSQVWDELTAIFADMGFAIAEGPDIETDDYNFTKLNFPEGHPAREMHDTFYFNPKD
CCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCCCCCHHHHHHCEEECCCC
DGSRLLLRTHTSPVQVRTMLSQKPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSH
CCCEEEEEECCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCEEEECCCC
LGHLKWILHEFCKAFFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLE
CHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHH
ILGCGMVHPNVLTACGLDPDVYQGFAWGMGIDRIAMLKYGMSDLRQLFEADSRWLNHYGF
HHHCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCC
KPLDIPTLAGGLSS
CCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA