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The map label for this gene is lipA-2 [H]

Identifier: 86610324

GI number: 86610324

Start: 3003801

End: 3004655

Strand: Direct

Name: lipA-2 [H]

Synonym: CYB_2905

Alternate gene names: 86610324

Gene position: 3003801-3004655 (Clockwise)

Preceding gene: 86610323

Following gene: 86610326

Centisome position: 98.59

GC content: 62.22

Gene sequence:

>855_bases
ATGTCGGTTAGCCCTCTTCCCAGTTGGGTCAAGCGTTCCATCGGCAAAGCCAGCGAGACTTCAGCGGTGCAGCAGGTGAT
CAAGCAGCGGGGCCTGCACACCATTTGCGAAGAGGGGCGCTGCCCAAACCGAGCCGAGTGCTACAGCCAAAAAACCGCTA
CATTTCTGTTGATGGGAGGGATCTGTACCCGCGCCTGCTCTTTTTGCCAGGTGGCCAGTGGACGGCCCGCACCGTTGGAT
CCCACAGAGCCGGAGAAAGTGGCCGAGGCGGTTGAGCTGCTGGGGCTCAGCTATGTGGTACTCACCTCGGTGGCCCGCGA
CGATCTGCCCGACCAGGGATCCGGTCGCTTTGTGGAGGTCATGGAGGCAATTCGGCGGCGCTGCCCCGGCACCCAGATCG
AGGTGCTCACCCCCGACTTTCGCGGGGATCGCGAGTGCATTGCCCGTGTGGTGGCTGCCCAGCCTGTCTGCTACAACCAC
AACATCGAAACGGTGCGACGCCTCTCCCAGGCAGTACGGCGCAGCTCCGGCTATGACCTGTCCTTGCGGGTTTTGCGAAC
GGTCAAGGAGCTGAATCCTGGATTACCCACCAAGTCTGGGCTGATGCTGGGGCATGGGGAGACCCGCGCAGAAGTGCTGG
AAACTTTCCGGGATCTGCTGGCGGTGGGATGCGATCGTCTGACTCTGGGGCAATACCTGCAGCCTTCCTTGGCCCATCGC
CCTGTGGAACGCTACTGGACTCCGGAGGAGTTTGCCGAGCTGGGCCAGATAGCCCGCCAGATGGGGTTTCGCCAGGTGCG
CTCAGGGCCATTGGTGCGCAGCTCCTACCACGCTGCCGAAATGGTTGAGAACTAG

Upstream 100 bases:

>100_bases
AGCTTCATCATGTCAGAATGGCAGAGCAGGCGCTGCATCCTTCGGCTGTAGAGGGGCGCAGTCTTGTTGCCGATACCCAG
TTAACGCAACGGTTCTCCCT

Downstream 100 bases:

>100_bases
GCCAGGGAGCTTGGCAGAGAGGCTGTGCAGTCTGTCATAGCCAACCTTTCCCGTCCGATCAAGGCACGAAGGCCCGCTTC
CAGGCCGAACGCTCGGGCTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGGICTRACSFCQVASGRPAPLD
PTEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEVMEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNH
NIETVRRLSQAVRRSSGYDLSLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCDRLTLGQYLQPSLAHR
PVERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN

Sequences:

>Translated_284_residues
MSVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGGICTRACSFCQVASGRPAPLD
PTEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEVMEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNH
NIETVRRLSQAVRRSSGYDLSLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCDRLTLGQYLQPSLAHR
PVERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN
>Mature_283_residues
SVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGGICTRACSFCQVASGRPAPLDP
TEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEVMEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNHN
IETVRRLSQAVRRSSGYDLSLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCDRLTLGQYLQPSLAHRP
VERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=43.6619718309859, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=42.8571428571429, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI1786846, Length=275, Percent_Identity=47.6363636363636, Blast_Score=269, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=43.3566433566434, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=287, Percent_Identity=43.9024390243902, Blast_Score=236, Evalue=3e-63,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=44.8763250883392, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 31488; Mature: 31356

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ICTRACSFCQVASGRPAPLDPTEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEV
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNHNIETVRRLSQAVRRSSGYDL
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCH
SLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCDRLTLGQYLQPSLAHR
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHC
PVERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN
CHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGG
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ICTRACSFCQVASGRPAPLDPTEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEV
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNHNIETVRRLSQAVRRSSGYDL
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCH
SLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCDRLTLGQYLQPSLAHR
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHC
PVERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN
CHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]