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The map label for this gene is map-2 [C]

Identifier: 86610048

GI number: 86610048

Start: 2710886

End: 2711659

Strand: Direct

Name: map-2 [C]

Synonym: CYB_2617

Alternate gene names: 86610048

Gene position: 2710886-2711659 (Clockwise)

Preceding gene: 86610047

Following gene: 86610049

Centisome position: 88.98

GC content: 59.56

Gene sequence:

>774_bases
ATGCAGCTTATTGAGATCAAATCTCGCCGCGAAGTGGAGAAAATGCGGCGGGCCGGTCGGGTGGTGGCCCAAGTATTGCA
GGAGATCGCGGAGCGCCTAGAGCCGGGCTGGACCACGGCAGATATCGATGCCTATGCCGAGAGACGGGTGGCCCAACTTA
ATGCTTTGCCCAGTTTCAAGGGCTACTACGGCTTTCCCGCCTGCGTCTGTGTCAGTGTCAACCATGAGGTGGTGCACGGG
ATCCCCAGTCCCCGTAAAGTGGTGCAGCCGGGAGATGTGGTCAAAGTGGATTTTGGTGCCATCGTCGAGGGCTGGCATGC
GGACTCCTGTATCACTATCGGCCTGGAACCCCTGAGCGACGCAGCTCGGGATCTGATCGATACTGCGGCCAAAGCCCTGC
AGACGGGCATCGACTGTGTGCGCCACGGCGTTTGGCTTCAGGATGTCTCGGGTGCCATTGAAGATTACATCGAGGGGCGG
GGCTATTCTGTGGTGAAGCAGTATGTTGGTCACGGGGTGGGGCGCAACCTGCACGAGGAGCCGCAGTTTCCCAACTATCG
CACCCGCGATCTGCCCAATCCCAAGCTGCGCTCCGGCATGACGGTGGCCATTGAGCCGATGGTGAATGCGGGGGGAGAGG
CAACCCGAGTGTTGCCAGACCAGTGGACGGTGGTCACGGTGGATGGCTCTTGGTCGGCTCAGTTTGAGCACACGGTCTTG
GTCACCGAGTCTGGAGCAGAAATTTTGACCGATCGCACGCCTTTTCAGTCCTAA

Upstream 100 bases:

>100_bases
CGATGGAATCCGTACAGGAACATCTGCAGGCTCTTTTGGAAGGCTTCAGGAGGACGGCGTGATCAGAGCAGGAGCTGGAA
ATCCCTCCCAGCGGCGGGGC

Downstream 100 bases:

>100_bases
GGGATCCCCTGATGAAGGACTGCAACGGTTAGGATAGAAGAGATGGAGTATATTTTCATTTCTTAACAGCGCGTGTCGTC
GCGGCTGTGGATTGTTCTGT

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAERLEPGWTTADIDAYAERRVAQLNALPSFKGYYGFPACVCVSVNHEVVHG
IPSPRKVVQPGDVVKVDFGAIVEGWHADSCITIGLEPLSDAARDLIDTAAKALQTGIDCVRHGVWLQDVSGAIEDYIEGR
GYSVVKQYVGHGVGRNLHEEPQFPNYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLPDQWTVVTVDGSWSAQFEHTVL
VTESGAEILTDRTPFQS

Sequences:

>Translated_257_residues
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAERLEPGWTTADIDAYAERRVAQLNALPSFKGYYGFPACVCVSVNHEVVHG
IPSPRKVVQPGDVVKVDFGAIVEGWHADSCITIGLEPLSDAARDLIDTAAKALQTGIDCVRHGVWLQDVSGAIEDYIEGR
GYSVVKQYVGHGVGRNLHEEPQFPNYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLPDQWTVVTVDGSWSAQFEHTVL
VTESGAEILTDRTPFQS
>Mature_257_residues
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAERLEPGWTTADIDAYAERRVAQLNALPSFKGYYGFPACVCVSVNHEVVHG
IPSPRKVVQPGDVVKVDFGAIVEGWHADSCITIGLEPLSDAARDLIDTAAKALQTGIDCVRHGVWLQDVSGAIEDYIEGR
GYSVVKQYVGHGVGRNLHEEPQFPNYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLPDQWTVVTVDGSWSAQFEHTVL
VTESGAEILTDRTPFQS

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=249, Percent_Identity=38.9558232931727, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI40385867, Length=248, Percent_Identity=35.0806451612903, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1786364, Length=250, Percent_Identity=40.8, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788728, Length=253, Percent_Identity=28.4584980237154, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71996291, Length=252, Percent_Identity=36.5079365079365, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323273, Length=254, Percent_Identity=36.6141732283465, Blast_Score=168, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24583427, Length=246, Percent_Identity=35.3658536585366, Blast_Score=173, Evalue=9e-44,
Organism=Drosophila melanogaster, GI21355531, Length=249, Percent_Identity=36.9477911646586, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 28304; Mature: 28304

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAERLEPGWTTADIDAYAERRVAQLNALPSFK
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
GYYGFPACVCVSVNHEVVHGIPSPRKVVQPGDVVKVDFGAIVEGWHADSCITIGLEPLSD
CCCCCCEEEEEECCHHHHCCCCCCHHHCCCCCEEEEEHHHHHCCCCCCCEEEECCCHHHH
AARDLIDTAAKALQTGIDCVRHGVWLQDVSGAIEDYIEGRGYSVVKQYVGHGVGRNLHEE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
PQFPNYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLPDQWTVVTVDGSWSAQFEHTVL
CCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCEEEEEE
VTESGAEILTDRTPFQS
EECCCCHHHCCCCCCCC
>Mature Secondary Structure
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAERLEPGWTTADIDAYAERRVAQLNALPSFK
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
GYYGFPACVCVSVNHEVVHGIPSPRKVVQPGDVVKVDFGAIVEGWHADSCITIGLEPLSD
CCCCCCEEEEEECCHHHHCCCCCCHHHCCCCCEEEEEHHHHHCCCCCCCEEEECCCHHHH
AARDLIDTAAKALQTGIDCVRHGVWLQDVSGAIEDYIEGRGYSVVKQYVGHGVGRNLHEE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
PQFPNYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLPDQWTVVTVDGSWSAQFEHTVL
CCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCEEEEEE
VTESGAEILTDRTPFQS
EECCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]