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The map label for this gene is metK [H]

Identifier: 86610022

GI number: 86610022

Start: 2690915

End: 2692159

Strand: Direct

Name: metK [H]

Synonym: CYB_2590

Alternate gene names: 86610022

Gene position: 2690915-2692159 (Clockwise)

Preceding gene: 86610021

Following gene: 86610028

Centisome position: 88.32

GC content: 60.96

Gene sequence:

>1245_bases
TTGTTTTCCTCGGAGTCGGTGACGGAGGGGCACCCCGACAAAATCTGCGATCAGATCTCCGATGCCATCGTGGATGCCCT
CTTGACGGCGGATCCCCTCAGTCGGGTGGCCGCCGAGGTGGTGGTCAATACGGGCCTGGTTCTGTTGACCGGGGAGATTA
CCTCACAAGCGCAGGTCAACTTCACCCGTCTGGTGCGGGACAAGGTGGCCGAAATTGGCTATACCGATGCCAAGAATGGG
TTTTGTGCAGAAAGCTGCGCGGTGTTGGTGGCTTTCGATGAGCAATCTCCCGATATTGCCCAAGGGGTGAACATGGCCCT
GGAAAGTCGCGGCTCCCAGGAAGATCAATTCGACTTGGTCGGGGCCGGGGATCAGGGGTTGATGTTTGGCTTTGCCTGCG
ATGAAACTCCCGAGTTGATGCCCTTGCCCATTAGCTTGGCCCACCGCCTCAGCCGCCAGTTGGCCACGGTTCGCAAAAAC
GGCACCCTGCCCTATCTGCGCCCGGATGGCAAAACGCAGGTGACTGTGGCCTATGAGGAAGGTCGCCCGGTGGGCATTCA
CACCCTGCTCATCTCCACCCAGCACACACCCACGATTGGCGCCATTACGGAAGAGGCGGCGGTGCAAGAGCGGATCCGCG
CCGATCTCTGGGAGGCAGTGGTCACGCCGGTGTTTGCCGAGCTGCCCATCAAGCCGGATAGCCACACCCGCTTCCTCACC
AATCCCACCGGCAAGTTTGTCATCGGCGGCCCTCAGGGAGATGCGGGCCTGACCGGACGCAAGATCATCGTCGATACCTA
CGGCGGCTATTCTCGCCATGGGGGTGGGGCCTTCTCCGGCAAGGATCCCACCAAGGTGGATCGCAGCGCTGCCTATGCGG
CTCGTTACGTGGCCAAGAACATCGTGGCAGCTGGCTTGGCGCAAAAATGTGAAGTGCAGGTGAGCTACGCCATTGGGGTA
GCCCGCCCCATCAACATTTTGGTGGAAACCTTTGGCACAGGGCGCATCCCCGACGAAGAGCTGTTGCGGCTGGTGCAGCG
CCACTTCGATCTGAGGCCGGCAGCCATTTTGGCCCAGTTCCAACTGCGGGAGCTGCCCCGCCAACGGGGGGGACGCTTCT
ACCAAAATGTAGCGGTCTATGGTCACTTCGGGCAAACTCACCTGGATCTGCCCTGGGAGCGCACCGATAAAGCCGCTCTG
CTGCGGGAGGAGGCTCTTGCCAAGGCGACTGCCCTCTTGGCCTAA

Upstream 100 bases:

>100_bases
CTCTTTCTCCGACGGATAATGAACGAGCGGGTGAGCCTTGTTTTTTGTTCAGGTTCTCAGTTCTAGCTTTTAAGGAGAAG
GTTCGTTGTCCAAGTCGTTT

Downstream 100 bases:

>100_bases
GAGGGGATCACTAGGTTGTGTTGATCTTTCATTTGAGCCAAAAGAGAGCACTGACGAGATAGAGAAAACTCAAGAAGTTC
CTGGCCAATTTGTCAAAACG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGLVLLTGEITSQAQVNFTRLVRDKVAEIGYTDAKNG
FCAESCAVLVAFDEQSPDIAQGVNMALESRGSQEDQFDLVGAGDQGLMFGFACDETPELMPLPISLAHRLSRQLATVRKN
GTLPYLRPDGKTQVTVAYEEGRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVTPVFAELPIKPDSHTRFLT
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLAQKCEVQVSYAIGV
ARPINILVETFGTGRIPDEELLRLVQRHFDLRPAAILAQFQLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAAL
LREEALAKATALLA

Sequences:

>Translated_414_residues
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGLVLLTGEITSQAQVNFTRLVRDKVAEIGYTDAKNG
FCAESCAVLVAFDEQSPDIAQGVNMALESRGSQEDQFDLVGAGDQGLMFGFACDETPELMPLPISLAHRLSRQLATVRKN
GTLPYLRPDGKTQVTVAYEEGRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVTPVFAELPIKPDSHTRFLT
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLAQKCEVQVSYAIGV
ARPINILVETFGTGRIPDEELLRLVQRHFDLRPAAILAQFQLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAAL
LREEALAKATALLA
>Mature_414_residues
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGLVLLTGEITSQAQVNFTRLVRDKVAEIGYTDAKNG
FCAESCAVLVAFDEQSPDIAQGVNMALESRGSQEDQFDLVGAGDQGLMFGFACDETPELMPLPISLAHRLSRQLATVRKN
GTLPYLRPDGKTQVTVAYEEGRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVTPVFAELPIKPDSHTRFLT
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLAQKCEVQVSYAIGV
ARPINILVETFGTGRIPDEELLRLVQRHFDLRPAAILAQFQLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAAL
LREEALAKATALLA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=398, Percent_Identity=53.5175879396985, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI4557737, Length=398, Percent_Identity=54.5226130653266, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI1789311, Length=408, Percent_Identity=56.8627450980392, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17538498, Length=398, Percent_Identity=52.0100502512563, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=418, Percent_Identity=50.9569377990431, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17538494, Length=398, Percent_Identity=51.7587939698493, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17551082, Length=413, Percent_Identity=50.1210653753027, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565911, Length=353, Percent_Identity=50.4249291784703, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI25145635, Length=373, Percent_Identity=49.3297587131367, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI32565905, Length=398, Percent_Identity=46.7336683417085, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=326, Percent_Identity=50.6134969325153, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=55.5555555555556, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323209, Length=398, Percent_Identity=50, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6320710, Length=401, Percent_Identity=49.3765586034913, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45552159, Length=398, Percent_Identity=50.5025125628141, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580529, Length=398, Percent_Identity=50.5025125628141, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580537, Length=398, Percent_Identity=50.5025125628141, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580533, Length=398, Percent_Identity=50.5025125628141, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580535, Length=398, Percent_Identity=50.5025125628141, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580531, Length=398, Percent_Identity=50.5025125628141, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580527, Length=398, Percent_Identity=50.5025125628141, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580525, Length=398, Percent_Identity=50.5025125628141, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580541, Length=400, Percent_Identity=49, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=53.6, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44820; Mature: 44820

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGLVLLTGEITSQAQVN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHH
FTRLVRDKVAEIGYTDAKNGFCAESCAVLVAFDEQSPDIAQGVNMALESRGSQEDQFDLV
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEE
GAGDQGLMFGFACDETPELMPLPISLAHRLSRQLATVRKNGTLPYLRPDGKTQVTVAYEE
ECCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEEEEC
GRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVTPVFAELPIKPDSHTRFLT
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IVAAGLAQKCEVQVSYAIGVARPINILVETFGTGRIPDEELLRLVQRHFDLRPAAILAQF
HHHHCCHHHCEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
QLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAALLREEALAKATALLA
HHHHHHHHHCCCEEEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGLVLLTGEITSQAQVN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHH
FTRLVRDKVAEIGYTDAKNGFCAESCAVLVAFDEQSPDIAQGVNMALESRGSQEDQFDLV
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEE
GAGDQGLMFGFACDETPELMPLPISLAHRLSRQLATVRKNGTLPYLRPDGKTQVTVAYEE
ECCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEEEEC
GRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVTPVFAELPIKPDSHTRFLT
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IVAAGLAQKCEVQVSYAIGVARPINILVETFGTGRIPDEELLRLVQRHFDLRPAAILAQF
HHHHCCHHHCEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
QLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAALLREEALAKATALLA
HHHHHHHHHCCCEEEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA