Click here to switch to the map view.

The map label for this gene is pfkA1 [H]

Identifier: 86609644

GI number: 86609644

Start: 2305535

End: 2306647

Strand: Direct

Name: pfkA1 [H]

Synonym: CYB_2199

Alternate gene names: 86609644

Gene position: 2305535-2306647 (Clockwise)

Preceding gene: 86609642

Following gene: 86609647

Centisome position: 75.67

GC content: 61.55

Gene sequence:

>1113_bases
ATGCCCAAACGTAAGCGAATCGGGATCCTCACCAGCGGCGGCGATTGTGGCGGCCTCAACGCCGTGATTCGAGCGGTGGT
GCGGCGGGCCCGCGACTACGACATGGAAGTTTATGGCATCAAAGATGCGACGGCAGGGCTCATGGCTCGACCGGTGGAGG
CGGAGCTGCTGGATGTGAAGCGGGTTTCCGGCATTTTGCGCTACGGCGGCACCATCTTGGGTACCGTGAACAAAGGCAAC
CCCTTCGCCTACCCTATGCCCGATGGCAGCTTGGTGGATCGCTCGGCAGAGGTGATCACCGGCTACCACCGGCTTGGGTT
GGATGCGCTGATCGGGGTGGGGGGGGATGGCAGCTTGGCCATTCTCCGGCAACTGGCCCTGCAGGGGGATTGGAACTTTG
TCGGGATCCCCAAGACCATCGACAACGATGTCGGTGCCACCGAGTATTCGGTGGGGTTCAACACAGCTCTGAGCGTGGCT
GTGGAAGCTCTAGATCAGCTCACCTACACGGCCATCAGCCACAGCCGAGTCATGGTCATGGAAGTCATGGGGCGGGATGC
CGGTCACATTGCCATCAGCGCCGGCATTGCCGGGGGGGCGCATGTGATTTTGATCCCGGAAATTCCCTACAAGCTGGAGA
ATGTCTGCAAAAGAATCCGCGACCGCAAAGAGCGGGGCAATAACTTCAGCATCGTGGTGGTGGCGGAGGCAGTTCGCACC
GAGTCGGGGGAGCCGGTGACCTACACCAACAGCCTGGGGCAGTCTCTCTACGGCGGCATCGGCCATTACATCAGCAATGA
GATCGCCCTGCGCACCGGCATCGAAACCCGGGTTATGGTTTTGGGCCACCTGCAGCGGGGAGGTACGCCTTCTCCTCTGG
ATCGCCTGCTGGGAGCAGCCTTTGGGGTGGCTGCTGTGGACTTGATCGCCCAAGGCAAATACGACCGCATGGTGGCCTGG
CAAAATCGGCGGGTGGTGGATGTGCCCCTGGCAGAGGCCATCTCCCAGTATCGGCGGGTGGAGGTGGACGGGGCTTTGAT
CGCCACGGCCATTGGGCTGAATACCTACGTGGGCGAGGTGGATCCCCAGCTTTTGCGCAGCATTTCCCCCTGA

Upstream 100 bases:

>100_bases
TGGATCACATCCGTAGTTTGCGATTGAGTAGGGATTCTCTGCAAGCGATATAGACCCTTATCTCTGTTCAGGATGTTCAC
CTTTCTTTAACTAGAGCTCT

Downstream 100 bases:

>100_bases
TCTTCCGGCAAAAACGACACGTGGGCAGAGACAATCTTCCAGCCGATATCGGGGAACCTGTACCACATCTGGCTTTGGCG
GCCCAAGCGAGGGATCCCGT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MPKRKRIGILTSGGDCGGLNAVIRAVVRRARDYDMEVYGIKDATAGLMARPVEAELLDVKRVSGILRYGGTILGTVNKGN
PFAYPMPDGSLVDRSAEVITGYHRLGLDALIGVGGDGSLAILRQLALQGDWNFVGIPKTIDNDVGATEYSVGFNTALSVA
VEALDQLTYTAISHSRVMVMEVMGRDAGHIAISAGIAGGAHVILIPEIPYKLENVCKRIRDRKERGNNFSIVVVAEAVRT
ESGEPVTYTNSLGQSLYGGIGHYISNEIALRTGIETRVMVLGHLQRGGTPSPLDRLLGAAFGVAAVDLIAQGKYDRMVAW
QNRRVVDVPLAEAISQYRRVEVDGALIATAIGLNTYVGEVDPQLLRSISP

Sequences:

>Translated_370_residues
MPKRKRIGILTSGGDCGGLNAVIRAVVRRARDYDMEVYGIKDATAGLMARPVEAELLDVKRVSGILRYGGTILGTVNKGN
PFAYPMPDGSLVDRSAEVITGYHRLGLDALIGVGGDGSLAILRQLALQGDWNFVGIPKTIDNDVGATEYSVGFNTALSVA
VEALDQLTYTAISHSRVMVMEVMGRDAGHIAISAGIAGGAHVILIPEIPYKLENVCKRIRDRKERGNNFSIVVVAEAVRT
ESGEPVTYTNSLGQSLYGGIGHYISNEIALRTGIETRVMVLGHLQRGGTPSPLDRLLGAAFGVAAVDLIAQGKYDRMVAW
QNRRVVDVPLAEAISQYRRVEVDGALIATAIGLNTYVGEVDPQLLRSISP
>Mature_369_residues
PKRKRIGILTSGGDCGGLNAVIRAVVRRARDYDMEVYGIKDATAGLMARPVEAELLDVKRVSGILRYGGTILGTVNKGNP
FAYPMPDGSLVDRSAEVITGYHRLGLDALIGVGGDGSLAILRQLALQGDWNFVGIPKTIDNDVGATEYSVGFNTALSVAV
EALDQLTYTAISHSRVMVMEVMGRDAGHIAISAGIAGGAHVILIPEIPYKLENVCKRIRDRKERGNNFSIVVVAEAVRTE
SGEPVTYTNSLGQSLYGGIGHYISNEIALRTGIETRVMVLGHLQRGGTPSPLDRLLGAAFGVAAVDLIAQGKYDRMVAWQ
NRRVVDVPLAEAISQYRRVEVDGALIATAIGLNTYVGEVDPQLLRSISP

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=365, Percent_Identity=32.3287671232877, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI266453768, Length=366, Percent_Identity=32.2404371584699, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI266453748, Length=366, Percent_Identity=32.2404371584699, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4505749, Length=366, Percent_Identity=32.2404371584699, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI266453619, Length=366, Percent_Identity=32.2404371584699, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI11321601, Length=365, Percent_Identity=31.7808219178082, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1790350, Length=336, Percent_Identity=38.3928571428571, Blast_Score=200, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17558788, Length=335, Percent_Identity=34.0298507462687, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI25147584, Length=370, Percent_Identity=27.8378378378378, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI72003601, Length=313, Percent_Identity=27.4760383386581, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321679, Length=374, Percent_Identity=31.0160427807487, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6323861, Length=369, Percent_Identity=30.3523035230352, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI28573326, Length=366, Percent_Identity=30.8743169398907, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24652337, Length=366, Percent_Identity=30.6010928961749, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17647809, Length=366, Percent_Identity=30.6010928961749, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 39609; Mature: 39478

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRKRIGILTSGGDCGGLNAVIRAVVRRARDYDMEVYGIKDATAGLMARPVEAELLDVK
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHH
RVSGILRYGGTILGTVNKGNPFAYPMPDGSLVDRSAEVITGYHRLGLDALIGVGGDGSLA
HHHHHHHHCCEEEEEECCCCCEEEECCCCCHHCCCHHHHHHHHHHCCHHEEECCCCCHHH
ILRQLALQGDWNFVGIPKTIDNDVGATEYSVGFNTALSVAVEALDQLTYTAISHSRVMVM
HHHHHHHCCCCCEEECCHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
EVMGRDAGHIAISAGIAGGAHVILIPEIPYKLENVCKRIRDRKERGNNFSIVVVAEAVRT
HHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHC
ESGEPVTYTNSLGQSLYGGIGHYISNEIALRTGIETRVMVLGHLQRGGTPSPLDRLLGAA
CCCCCEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEHHCCCCCCCHHHHHHHHH
FGVAAVDLIAQGKYDRMVAWQNRRVVDVPLAEAISQYRRVEVDGALIATAIGLNTYVGEV
HHHHHHHHHHCCCCCCEEEECCCEEEECCHHHHHHHHHEEECCCCEEEHHHHHHHHHCCC
DPQLLRSISP
CHHHHHCCCC
>Mature Secondary Structure 
PKRKRIGILTSGGDCGGLNAVIRAVVRRARDYDMEVYGIKDATAGLMARPVEAELLDVK
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHH
RVSGILRYGGTILGTVNKGNPFAYPMPDGSLVDRSAEVITGYHRLGLDALIGVGGDGSLA
HHHHHHHHCCEEEEEECCCCCEEEECCCCCHHCCCHHHHHHHHHHCCHHEEECCCCCHHH
ILRQLALQGDWNFVGIPKTIDNDVGATEYSVGFNTALSVAVEALDQLTYTAISHSRVMVM
HHHHHHHCCCCCEEECCHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
EVMGRDAGHIAISAGIAGGAHVILIPEIPYKLENVCKRIRDRKERGNNFSIVVVAEAVRT
HHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHC
ESGEPVTYTNSLGQSLYGGIGHYISNEIALRTGIETRVMVLGHLQRGGTPSPLDRLLGAA
CCCCCEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEHHCCCCCCCHHHHHHHHH
FGVAAVDLIAQGKYDRMVAWQNRRVVDVPLAEAISQYRRVEVDGALIATAIGLNTYVGEV
HHHHHHHHHHCCCCCCEEEECCCEEEECCHHHHHHHHHEEECCCCEEEHHHHHHHHHCCC
DPQLLRSISP
CHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]