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The map label for this gene is ribAB [H]

Identifier: 86609524

GI number: 86609524

Start: 2172472

End: 2173725

Strand: Direct

Name: ribAB [H]

Synonym: CYB_2077

Alternate gene names: 86609524

Gene position: 2172472-2173725 (Clockwise)

Preceding gene: 86609523

Following gene: 86609528

Centisome position: 71.31

GC content: 60.93

Gene sequence:

>1254_bases
ATGAGTGCCATCCCGCAGGCCGAGACAGAGACGATCTTCCACTCCATCCCCGAAGCCCTCGAAGACCTCAAGTGTGGTCG
CATGGTCATCGTGGTCGATGACGAAAACCGAGAAAACGAAGGGGATCTGGTCTGTGCTGCCCAGTTTGCCACTCCGCAGG
CCATCAACTTCATGGCCACCTACGGGCGGGGCCTTATCTGCCTGGCCATGCAGGGATCCCGCCTCGATGAGCTGAAGATC
CCCCTGATGGTGGGGCAGAACACCGACAGCAACCAAACTGCCTTCACCGTCAGCATTGATGCTGGGCCGGAAGCCGGAGT
CTCCACCGGCATTTCCGCTGCCGATCGCTCTCGCACCATTCAGGCGGCCATTCACCCGCACACGCGACCGGAAGATCTGC
GCCGGCCTGGGCATGTCTTTCCGCTGCGGGCACGGGAGGGCGGAGTACTCAAACGGGCTGGCCATACGGAAGCAGCGGTG
GATCTGGCGATGTTGGCCGGGCTGTATCCGGCAGGGGTGATTTGCGAGATCCAAAACCCGGATGGCTCCATGGCCCGCCT
GCCCCAGTTGATCGAATATGCCCGTCAGCACGATTTGAAGATCATCACCATCGCCCACCTCATCGAGTACCGCCTGCGCA
CCGAACAACTGGTTCACCGAGAGGCTGCTGCTATCCTGCCGACGGAGTTCGGCCAGTTTCGGGTTTACGGCTACCGCGAT
AGCCTCAACCAAGTCGAGCACCTGGCTCTGGTGAAAGGGGATCCGGACCAATTTGCCTCTGAGCCCGTCTTGGTGCGCGT
CCATGTGGAGTGTCCCCTAGGGGATGCGCTGGGATCCCTGCGCTGCGATTGTCGCCGCCAATTGACAGCGGCCCTGAAAA
TGATCGACCACCAGGGCAAGGGGGTGCTGGTGTACCTGCGCCGGCAAGAGCAGGAGCTGAGTCTCATCGAGCAAATCAAG
TCCTACGCCCGGCAAGATCTGGGACTTTCTGAAACCGCGCCACCGGTGGCGATGGACTTGCGCGATTACGGCATCGGCGC
CCAGATCCTACGGGATGTAGGGGTGCGCAAAATGCGCCTGATCAGCAACACCTCTCGCCACATCAGCGGCTTGAAAGGAT
TTGGCCTGGAAGTGGCCGAGCGCGTGCCCCTGCTGGTGGAAGAGCCGGATCTCAATTTGCATGTGGAGCCGTCCTGTTCC
CTGAAACTGTTGCTGCACGACACTGGCGCAACCGATTCCGGCTCAAGCCTGTAG

Upstream 100 bases:

>100_bases
GGATCCCTACCTGTCGCCCGGTTTGCTCTAGCATAGATCCGGCAGATCCGGTGCCCAGAGCCGGCTGTGCCAGGTTTTCC
CTTTTTGCGTCCCCTTTTCC

Downstream 100 bases:

>100_bases
CGCGTTGTAGGGCCGAGGGCTATGGTGGAACAAAGACTGGGGTGCAGCCGGCTCGCCATCCACCACATAAGCCTTGGATC
CCCAACCCCAAAAAGACCAG

Product: riboflavin biosynthesis protein RibA

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSAIPQAETETIFHSIPEALEDLKCGRMVIVVDDENRENEGDLVCAAQFATPQAINFMATYGRGLICLAMQGSRLDELKI
PLMVGQNTDSNQTAFTVSIDAGPEAGVSTGISAADRSRTIQAAIHPHTRPEDLRRPGHVFPLRAREGGVLKRAGHTEAAV
DLAMLAGLYPAGVICEIQNPDGSMARLPQLIEYARQHDLKIITIAHLIEYRLRTEQLVHREAAAILPTEFGQFRVYGYRD
SLNQVEHLALVKGDPDQFASEPVLVRVHVECPLGDALGSLRCDCRRQLTAALKMIDHQGKGVLVYLRRQEQELSLIEQIK
SYARQDLGLSETAPPVAMDLRDYGIGAQILRDVGVRKMRLISNTSRHISGLKGFGLEVAERVPLLVEEPDLNLHVEPSCS
LKLLLHDTGATDSGSSL

Sequences:

>Translated_417_residues
MSAIPQAETETIFHSIPEALEDLKCGRMVIVVDDENRENEGDLVCAAQFATPQAINFMATYGRGLICLAMQGSRLDELKI
PLMVGQNTDSNQTAFTVSIDAGPEAGVSTGISAADRSRTIQAAIHPHTRPEDLRRPGHVFPLRAREGGVLKRAGHTEAAV
DLAMLAGLYPAGVICEIQNPDGSMARLPQLIEYARQHDLKIITIAHLIEYRLRTEQLVHREAAAILPTEFGQFRVYGYRD
SLNQVEHLALVKGDPDQFASEPVLVRVHVECPLGDALGSLRCDCRRQLTAALKMIDHQGKGVLVYLRRQEQELSLIEQIK
SYARQDLGLSETAPPVAMDLRDYGIGAQILRDVGVRKMRLISNTSRHISGLKGFGLEVAERVPLLVEEPDLNLHVEPSCS
LKLLLHDTGATDSGSSL
>Mature_416_residues
SAIPQAETETIFHSIPEALEDLKCGRMVIVVDDENRENEGDLVCAAQFATPQAINFMATYGRGLICLAMQGSRLDELKIP
LMVGQNTDSNQTAFTVSIDAGPEAGVSTGISAADRSRTIQAAIHPHTRPEDLRRPGHVFPLRAREGGVLKRAGHTEAAVD
LAMLAGLYPAGVICEIQNPDGSMARLPQLIEYARQHDLKIITIAHLIEYRLRTEQLVHREAAAILPTEFGQFRVYGYRDS
LNQVEHLALVKGDPDQFASEPVLVRVHVECPLGDALGSLRCDCRRQLTAALKMIDHQGKGVLVYLRRQEQELSLIEQIKS
YARQDLGLSETAPPVAMDLRDYGIGAQILRDVGVRKMRLISNTSRHISGLKGFGLEVAERVPLLVEEPDLNLHVEPSCSL
KLLLHDTGATDSGSSL

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=201, Percent_Identity=49.7512437810945, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1787533, Length=179, Percent_Identity=39.6648044692737, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320695, Length=207, Percent_Identity=41.0628019323672, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6319438, Length=143, Percent_Identity=34.965034965035, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 45753; Mature: 45622

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIPQAETETIFHSIPEALEDLKCGRMVIVVDDENRENEGDLVCAAQFATPQAINFMAT
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHH
YGRGLICLAMQGSRLDELKIPLMVGQNTDSNQTAFTVSIDAGPEAGVSTGISAADRSRTI
CCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHCCCCEE
QAAIHPHTRPEDLRRPGHVFPLRAREGGVLKRAGHTEAAVDLAMLAGLYPAGVICEIQNP
EEEECCCCCHHHHCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCCEEEEEECC
DGSMARLPQLIEYARQHDLKIITIAHLIEYRLRTEQLVHREAAAILPTEFGQFRVYGYRD
CCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHH
SLNQVEHLALVKGDPDQFASEPVLVRVHVECPLGDALGSLRCDCRRQLTAALKMIDHQGK
HHHHHHEEEEEECCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GVLVYLRRQEQELSLIEQIKSYARQDLGLSETAPPVAMDLRDYGIGAQILRDVGVRKMRL
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHCCCHHHHHHHHCHHHHHH
ISNTSRHISGLKGFGLEVAERVPLLVEEPDLNLHVEPSCSLKLLLHDTGATDSGSSL
HHCCHHHHCCCCCCCHHHHHCCCEEEECCCCCEEECCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SAIPQAETETIFHSIPEALEDLKCGRMVIVVDDENRENEGDLVCAAQFATPQAINFMAT
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHH
YGRGLICLAMQGSRLDELKIPLMVGQNTDSNQTAFTVSIDAGPEAGVSTGISAADRSRTI
CCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHCCCCEE
QAAIHPHTRPEDLRRPGHVFPLRAREGGVLKRAGHTEAAVDLAMLAGLYPAGVICEIQNP
EEEECCCCCHHHHCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCCEEEEEECC
DGSMARLPQLIEYARQHDLKIITIAHLIEYRLRTEQLVHREAAAILPTEFGQFRVYGYRD
CCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHH
SLNQVEHLALVKGDPDQFASEPVLVRVHVECPLGDALGSLRCDCRRQLTAALKMIDHQGK
HHHHHHEEEEEECCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GVLVYLRRQEQELSLIEQIKSYARQDLGLSETAPPVAMDLRDYGIGAQILRDVGVRKMRL
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHCCCHHHHHHHHCHHHHHH
ISNTSRHISGLKGFGLEVAERVPLLVEEPDLNLHVEPSCSLKLLLHDTGATDSGSSL
HHCCHHHHCCCCCCCHHHHHCCCEEEECCCCCEEECCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]