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The map label for this gene is ybiK [C]

Identifier: 86609454

GI number: 86609454

Start: 2110969

End: 2111847

Strand: Direct

Name: ybiK [C]

Synonym: CYB_2005

Alternate gene names: 86609454

Gene position: 2110969-2111847 (Clockwise)

Preceding gene: 86609452

Following gene: 86609455

Centisome position: 69.29

GC content: 62.68

Gene sequence:

>879_bases
ATGCCTCAGCCCAAACTGATCATCCACGGCGGAGCCGGCAGCGCCCTGGAAGGGGAAAGCAGCCTGGAAAAAGTGAGGGG
ATCCCTGCAGCAGGTGGTGGAGAGCACCTACGAGCTGCTGTTGGCTGGGCAGAGTGCCGAGGCGGCGGTGATGCATGCCT
GCCAGTTGCTGGAGGATGATCCGCTCTACAATGCCGGGACGGGGTCGGTCTTGCAATCGGATGGGCAAATCCGCATGAGC
GCCGCTCTCATGAACGGGGCCACCCAGTCTTTTAGCGGGGTGATCAACGTCAGCCGAGTTCAGCATCCCATCCTCATGGC
CCAGGCTCTGCAATCAAGCCCGGATCGCGTGGTGGCCGAACAAGGGGCAGCAGAGCTGGCGCGGGAGCTGCAGTTGCCGG
TCTATGACCCGGCCACTCTCAAGCGGGTTCAAGAGTGGGTGCAGTTGCGTCAAACCCATCCCCCGACCAGGGTTCTCACT
GCTCTGGCCATGGGCACGGTGGGGGCGGTGGCGCTGGATCAACAGGGGCAAATCTGTGCCGGCACCTCCACCGGCGGGCG
GGGGTTTGAACGGGTGGGGCGAGTAAGCGATTCGGCCACTCCAGCGGGAACCTATGCCAGTGCGGCGGCGGGGGTCAGTT
GTACTGGTGTCGGGGAAGATATTTTGGATGAGAGCTTGGCGGCGCGGATTGTGATTCGAGTGGAAGATGGCCTCAGCCTG
AAGGCTGCCATGGCCAAGTCTTTTCAAGAGGCCCGCCAACGTCGCCGCTCCTTTGGGGCCATTGCTCTATCGCGCTCGGG
CGAGATTGCTTGGGATACCACCACCGACCTACTGCTGGCCGCCTATCACGATGGGCAGGGGATCGGCTTGACGATCTAA

Upstream 100 bases:

>100_bases
GCGTTGGGATCCCTGGGATGGAGCAGATGCATGGGTGGATGTTGTACCCGAAAATCAAAATATAGTCTTCCACTGTTCTC
ACAAGGTAACCGATCTCATC

Downstream 100 bases:

>100_bases
ACTTCTAGCCGGGCTTGCGGGATTAGACCTGGACAAGCCTGAGGCGGTTGGATAAGAGTAGGATAAGAGTAGAAATAGTA
CTTAAACAAACCAAGTCTGC

Product: asparaginase 2 family protein

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MPQPKLIIHGGAGSALEGESSLEKVRGSLQQVVESTYELLLAGQSAEAAVMHACQLLEDDPLYNAGTGSVLQSDGQIRMS
AALMNGATQSFSGVINVSRVQHPILMAQALQSSPDRVVAEQGAAELARELQLPVYDPATLKRVQEWVQLRQTHPPTRVLT
ALAMGTVGAVALDQQGQICAGTSTGGRGFERVGRVSDSATPAGTYASAAAGVSCTGVGEDILDESLAARIVIRVEDGLSL
KAAMAKSFQEARQRRRSFGAIALSRSGEIAWDTTTDLLLAAYHDGQGIGLTI

Sequences:

>Translated_292_residues
MPQPKLIIHGGAGSALEGESSLEKVRGSLQQVVESTYELLLAGQSAEAAVMHACQLLEDDPLYNAGTGSVLQSDGQIRMS
AALMNGATQSFSGVINVSRVQHPILMAQALQSSPDRVVAEQGAAELARELQLPVYDPATLKRVQEWVQLRQTHPPTRVLT
ALAMGTVGAVALDQQGQICAGTSTGGRGFERVGRVSDSATPAGTYASAAAGVSCTGVGEDILDESLAARIVIRVEDGLSL
KAAMAKSFQEARQRRRSFGAIALSRSGEIAWDTTTDLLLAAYHDGQGIGLTI
>Mature_291_residues
PQPKLIIHGGAGSALEGESSLEKVRGSLQQVVESTYELLLAGQSAEAAVMHACQLLEDDPLYNAGTGSVLQSDGQIRMSA
ALMNGATQSFSGVINVSRVQHPILMAQALQSSPDRVVAEQGAAELARELQLPVYDPATLKRVQEWVQLRQTHPPTRVLTA
LAMGTVGAVALDQQGQICAGTSTGGRGFERVGRVSDSATPAGTYASAAAGVSCTGVGEDILDESLAARIVIRVEDGLSLK
AAMAKSFQEARQRRRSFGAIALSRSGEIAWDTTTDLLLAAYHDGQGIGLTI

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Probably performs the final step in the degradation of the reserve polymer cyanophycin (depolymerizes the building block L-beta-Asp-Arg). Also has L- asparagin

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=292, Percent_Identity=33.2191780821918, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI145275200, Length=292, Percent_Identity=33.2191780821918, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI98991769, Length=262, Percent_Identity=26.3358778625954, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787050, Length=300, Percent_Identity=34, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18921183, Length=314, Percent_Identity=34.0764331210191, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21357893, Length=327, Percent_Identity=28.7461773700306, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 30595; Mature: 30464

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQPKLIIHGGAGSALEGESSLEKVRGSLQQVVESTYELLLAGQSAEAAVMHACQLLEDD
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PLYNAGTGSVLQSDGQIRMSAALMNGATQSFSGVINVSRVQHPILMAQALQSSPDRVVAE
CCCCCCCCCCEECCCCEEEHHHHHHCCHHHHHHHEEHHHHHHHHHHHHHHHCCCCHHHHH
QGAAELARELQLPVYDPATLKRVQEWVQLRQTHPPTRVLTALAMGTVGAVALDQQGQICA
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCCEEE
GTSTGGRGFERVGRVSDSATPAGTYASAAAGVSCTGVGEDILDESLAARIVIRVEDGLSL
CCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCEECCCCHHHHHHHHHEEEEEEECCCCCH
KAAMAKSFQEARQRRRSFGAIALSRSGEIAWDTTTDLLLAAYHDGQGIGLTI
HHHHHHHHHHHHHHHHHHCEEEEECCCCEEECCHHHEEEEEEECCCCCEEEC
>Mature Secondary Structure 
PQPKLIIHGGAGSALEGESSLEKVRGSLQQVVESTYELLLAGQSAEAAVMHACQLLEDD
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PLYNAGTGSVLQSDGQIRMSAALMNGATQSFSGVINVSRVQHPILMAQALQSSPDRVVAE
CCCCCCCCCCEECCCCEEEHHHHHHCCHHHHHHHEEHHHHHHHHHHHHHHHCCCCHHHHH
QGAAELARELQLPVYDPATLKRVQEWVQLRQTHPPTRVLTALAMGTVGAVALDQQGQICA
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCCEEE
GTSTGGRGFERVGRVSDSATPAGTYASAAAGVSCTGVGEDILDESLAARIVIRVEDGLSL
CCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCEECCCCHHHHHHHHHEEEEEEECCCCCH
KAAMAKSFQEARQRRRSFGAIALSRSGEIAWDTTTDLLLAAYHDGQGIGLTI
HHHHHHHHHHHHHHHHHHCEEEEECCCCEEECCHHHEEEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]