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The map label for this gene is eda [H]

Identifier: 86609355

GI number: 86609355

Start: 1985630

End: 1986343

Strand: Direct

Name: eda [H]

Synonym: CYB_1899

Alternate gene names: 86609355

Gene position: 1985630-1986343 (Clockwise)

Preceding gene: 86609353

Following gene: 86609356

Centisome position: 65.17

GC content: 61.2

Gene sequence:

>714_bases
ATGGCATTCGAGGATGCTGAGTGCAGTTTCAGAAAAGGGTCACAGCACCCCTGGTTACTCCATCTGTGGCAGCAGCCGCT
GATTGCCGTGATCCGCCTCTGGGCCAGCCCAGCCCAAGCTCTCGAGGGAGCCATGCTGGCCATCCGAGCCGGGATCCGCC
ACATTGAAATCACCACGCAAGTTCCCCAATATTTGGAGCTGATTGCCCAACTGCGACAAGACCACCCGCAGTGTTGGATA
GGGGTGGGCACTGTTCTCGATCGGCAGGTGGCGGAGCAGGCTTGGCAGGCAGGAGCCCAGTTTTGCGTTTGCCCCTTCAC
CGATGAGCAAGTTATCCGCTGGGGTCGGGAGGTGGGGCTGCCCATCGTGCCCGGTGCCCTCACCCCCAGCGAGATCTGGA
GGGCGTGGCAGGCGGGGGCAACGGCAGTGAAGGTGTTTCCGGTGGAGAGCATGGGGGGATCCCGCTACCTGCGCCATCTA
CAATCGGTGCTGGGATCCCTGCCGTTGATCCCCACCGGGGGAGTCACCTTGGCCAAGGCAGCGGAATACCTGCAAGCAGG
GGCCTGGGCAGTGGGCATTGCCGGGGATCTGTTCCCGCCAGAGGATTTGCATTCGGGCTGCCATGAGCTGGAGAATCGAC
TACGACAGGCGCTGAAACAGCTGGCAGAGCAAAGAAGCAAAACCTGGCCCAATCTGGGCAAACCAGTAGAATAA

Upstream 100 bases:

>100_bases
ATTATTTCATTGGCAGGCTGACTTTTTCGTTTTTGGATTGCAATTAAACTTAAGCTAAACCCAAGCAACTGCAAAACACT
GAGCGGGTATTTTCCTACAG

Downstream 100 bases:

>100_bases
GGGGCGTGGTGTGAGTGAGGCCCGCATGGATGGATTTTTAGATCTCTATCTGCAATTCAACATCCCAAGCCCGATTGAAG
AAGCCACTTGGCGTCATTGG

Product: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAFEDAECSFRKGSQHPWLLHLWQQPLIAVIRLWASPAQALEGAMLAIRAGIRHIEITTQVPQYLELIAQLRQDHPQCWI
GVGTVLDRQVAEQAWQAGAQFCVCPFTDEQVIRWGREVGLPIVPGALTPSEIWRAWQAGATAVKVFPVESMGGSRYLRHL
QSVLGSLPLIPTGGVTLAKAAEYLQAGAWAVGIAGDLFPPEDLHSGCHELENRLRQALKQLAEQRSKTWPNLGKPVE

Sequences:

>Translated_237_residues
MAFEDAECSFRKGSQHPWLLHLWQQPLIAVIRLWASPAQALEGAMLAIRAGIRHIEITTQVPQYLELIAQLRQDHPQCWI
GVGTVLDRQVAEQAWQAGAQFCVCPFTDEQVIRWGREVGLPIVPGALTPSEIWRAWQAGATAVKVFPVESMGGSRYLRHL
QSVLGSLPLIPTGGVTLAKAAEYLQAGAWAVGIAGDLFPPEDLHSGCHELENRLRQALKQLAEQRSKTWPNLGKPVE
>Mature_236_residues
AFEDAECSFRKGSQHPWLLHLWQQPLIAVIRLWASPAQALEGAMLAIRAGIRHIEITTQVPQYLELIAQLRQDHPQCWIG
VGTVLDRQVAEQAWQAGAQFCVCPFTDEQVIRWGREVGLPIVPGALTPSEIWRAWQAGATAVKVFPVESMGGSRYLRHLQ
SVLGSLPLIPTGGVTLAKAAEYLQAGAWAVGIAGDLFPPEDLHSGCHELENRLRQALKQLAEQRSKTWPNLGKPVE

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=180, Percent_Identity=33.8888888888889, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788156, Length=174, Percent_Identity=29.3103448275862, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.2.14 [H]

Molecular weight: Translated: 26203; Mature: 26072

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEDAECSFRKGSQHPWLLHLWQQPLIAVIRLWASPAQALEGAMLAIRAGIRHIEITTQ
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEHHH
VPQYLELIAQLRQDHPQCWIGVGTVLDRQVAEQAWQAGAQFCVCPFTDEQVIRWGREVGL
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCC
PIVPGALTPSEIWRAWQAGATAVKVFPVESMGGSRYLRHLQSVLGSLPLIPTGGVTLAKA
CCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AEYLQAGAWAVGIAGDLFPPEDLHSGCHELENRLRQALKQLAEQRSKTWPNLGKPVE
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AFEDAECSFRKGSQHPWLLHLWQQPLIAVIRLWASPAQALEGAMLAIRAGIRHIEITTQ
CCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEHHH
VPQYLELIAQLRQDHPQCWIGVGTVLDRQVAEQAWQAGAQFCVCPFTDEQVIRWGREVGL
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCC
PIVPGALTPSEIWRAWQAGATAVKVFPVESMGGSRYLRHLQSVLGSLPLIPTGGVTLAKA
CCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AEYLQAGAWAVGIAGDLFPPEDLHSGCHELENRLRQALKQLAEQRSKTWPNLGKPVE
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6988426; 7161801; 974067 [H]