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The map label for this gene is thiE [H]

Identifier: 86609272

GI number: 86609272

Start: 1900081

End: 1901127

Strand: Direct

Name: thiE [H]

Synonym: CYB_1815

Alternate gene names: 86609272

Gene position: 1900081-1901127 (Clockwise)

Preceding gene: 86609271

Following gene: 86609273

Centisome position: 62.37

GC content: 63.13

Gene sequence:

>1047_bases
ATGAAACCCCAGGCCGTCGCCCGTATTCTGGATGCCAACCTCGATCGAGCTCGTGAAGGACTACGGGTATTGGAAGAATG
GTTTCGTTTCGGCCTGGAAGAGGGAAAGCTCTCTGCCGAATGCAAGGCCATGCGCCAAGCCCTGGCCCGCTGGCACAGCG
ATCCCCTGCGTTTGGCCCGCGATACTTCCGCCGATCCAGGCACCCAGATCTCCCACCCGCAAGAGGAGCAACGGCGGGAT
CTCCGCCACCTCTTGCAAGCCAATTGCAGCCGTGTGCAAGAAGCCCTGCGGGTGTTAGAAGAATACGGCAAGTTGGCCGA
ATCCCACCACTGGGTGGCCCCTGGCCTGGCCAAGCTGGCCAAAGACATGCGCTATCGGCTCTACGTCTTGGAAAGTGAAC
TGCTGGGCGGATCCCTGCGGCAGCGTCTCCTGGCAGCCCATCTTTATTTGGTGACCAGCCCTGTGCCCCACTGGCTAGAG
GTGGTGGAAAAATCCCTGCGGGGCGGGGTGACCCTGGTGCAATATCGCCGCAAAAACCTCCCCGACGGCGAGATGCTGCG
GGATCTCAAACAGCTGCGGCAGCTGTGCGACCGCTACCAGGCGCTGATGATCGTCAACGACCGGGTGGACTTGGCTCTGG
TCAGCCAGGCTGATGGGGTTCACCTGGGCCAGACGGATCTGCCGGTCGCCCAAGCTCGGCACCTCCTGGGATCCCAGCGG
CTCATTGGCCTGTCCACCACCAACGCCGAAGAGCTGGCCCAAGCCCTGAACAGCGACGTGGACTACATTGGCGTCGGCCC
CGTTTACCCCACCCCCACCAAAGCGGAGAAACCCCCTGCCGGCCTGGAGTACGTGCGCCTGGCTGCCGAGAAAGCCCACC
TGCCCTGGTTTGCCATCGGCGGCATCGATCCCCACAACCTGCCCGAGGTGCGTCGAGCTGGGGCCACCCGTGTAGCTGTG
GTGCGGGCTTTGATGGAAGCTGCCGATCCCACCCAGACAGCCCGCACCATGCTGGCCGAACTGCAAGGGATCCCTGCCCT
ATCCTAA

Upstream 100 bases:

>100_bases
TCTCCACCCTACTGACTTGCTTCCGGCTAAGGGTAGGACGGGGCTTTAGGCGCATTTTTTTGGTAAAAATTAAGCAACTC
TCTTCCCACGTATAGGGAAC

Downstream 100 bases:

>100_bases
AGATCAACCGTGAAGCTCAACCGTGAGCATTTTGCTGCTTATTGGCTGTTCCAATCCAAATGGTTTCTGAACAAGTGAGA
GGAAGATGAAGTAAGGATGT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKPQAVARILDANLDRAREGLRVLEEWFRFGLEEGKLSAECKAMRQALARWHSDPLRLARDTSADPGTQISHPQEEQRRD
LRHLLQANCSRVQEALRVLEEYGKLAESHHWVAPGLAKLAKDMRYRLYVLESELLGGSLRQRLLAAHLYLVTSPVPHWLE
VVEKSLRGGVTLVQYRRKNLPDGEMLRDLKQLRQLCDRYQALMIVNDRVDLALVSQADGVHLGQTDLPVAQARHLLGSQR
LIGLSTTNAEELAQALNSDVDYIGVGPVYPTPTKAEKPPAGLEYVRLAAEKAHLPWFAIGGIDPHNLPEVRRAGATRVAV
VRALMEAADPTQTARTMLAELQGIPALS

Sequences:

>Translated_348_residues
MKPQAVARILDANLDRAREGLRVLEEWFRFGLEEGKLSAECKAMRQALARWHSDPLRLARDTSADPGTQISHPQEEQRRD
LRHLLQANCSRVQEALRVLEEYGKLAESHHWVAPGLAKLAKDMRYRLYVLESELLGGSLRQRLLAAHLYLVTSPVPHWLE
VVEKSLRGGVTLVQYRRKNLPDGEMLRDLKQLRQLCDRYQALMIVNDRVDLALVSQADGVHLGQTDLPVAQARHLLGSQR
LIGLSTTNAEELAQALNSDVDYIGVGPVYPTPTKAEKPPAGLEYVRLAAEKAHLPWFAIGGIDPHNLPEVRRAGATRVAV
VRALMEAADPTQTARTMLAELQGIPALS
>Mature_348_residues
MKPQAVARILDANLDRAREGLRVLEEWFRFGLEEGKLSAECKAMRQALARWHSDPLRLARDTSADPGTQISHPQEEQRRD
LRHLLQANCSRVQEALRVLEEYGKLAESHHWVAPGLAKLAKDMRYRLYVLESELLGGSLRQRLLAAHLYLVTSPVPHWLE
VVEKSLRGGVTLVQYRRKNLPDGEMLRDLKQLRQLCDRYQALMIVNDRVDLALVSQADGVHLGQTDLPVAQARHLLGSQR
LIGLSTTNAEELAQALNSDVDYIGVGPVYPTPTKAEKPPAGLEYVRLAAEKAHLPWFAIGGIDPHNLPEVRRAGATRVAV
VRALMEAADPTQTARTMLAELQGIPALS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=193, Percent_Identity=36.2694300518135, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=179, Percent_Identity=36.3128491620112, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 38837; Mature: 38837

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQAVARILDANLDRAREGLRVLEEWFRFGLEEGKLSAECKAMRQALARWHSDPLRLAR
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
DTSADPGTQISHPQEEQRRDLRHLLQANCSRVQEALRVLEEYGKLAESHHWVAPGLAKLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KDMRYRLYVLESELLGGSLRQRLLAAHLYLVTSPVPHWLEVVEKSLRGGVTLVQYRRKNL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCC
PDGEMLRDLKQLRQLCDRYQALMIVNDRVDLALVSQADGVHLGQTDLPVAQARHLLGSQR
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
LIGLSTTNAEELAQALNSDVDYIGVGPVYPTPTKAEKPPAGLEYVRLAAEKAHLPWFAIG
EEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
GIDPHNLPEVRRAGATRVAVVRALMEAADPTQTARTMLAELQGIPALS
CCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKPQAVARILDANLDRAREGLRVLEEWFRFGLEEGKLSAECKAMRQALARWHSDPLRLAR
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
DTSADPGTQISHPQEEQRRDLRHLLQANCSRVQEALRVLEEYGKLAESHHWVAPGLAKLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KDMRYRLYVLESELLGGSLRQRLLAAHLYLVTSPVPHWLEVVEKSLRGGVTLVQYRRKNL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCC
PDGEMLRDLKQLRQLCDRYQALMIVNDRVDLALVSQADGVHLGQTDLPVAQARHLLGSQR
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
LIGLSTTNAEELAQALNSDVDYIGVGPVYPTPTKAEKPPAGLEYVRLAAEKAHLPWFAIG
EEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
GIDPHNLPEVRRAGATRVAVVRALMEAADPTQTARTMLAELQGIPALS
CCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA