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The map label for this gene is potD-B [H]

Identifier: 86609220

GI number: 86609220

Start: 1846142

End: 1847293

Strand: Reverse

Name: potD-B [H]

Synonym: CYB_1762

Alternate gene names: 86609220

Gene position: 1847293-1846142 (Counterclockwise)

Preceding gene: 86609221

Following gene: 86609219

Centisome position: 60.63

GC content: 61.98

Gene sequence:

>1152_bases
GTGGGCGAGATACGGCTGAGCCGACGTGAGCTTCTACAGGGGATCCTGCTGGGGCTAATGGCGGCAGGATGTGGGGGGCC
GGATGCACACCTGCCTCTTGTCGTGGCGCTCAAGCAAGGGATCCCCGCCAGTTTGCTCAACGAGTTTCGCCGCCGCACCC
AGGCCCGCTTTCGGCTGCAACTGTTGGATGAGCGGGATCAGTTGTTGGCCTATCTGCAGCAAAGCGTCCAGCCGGCTGAT
GCAGCCTGGTGGGATCCGGGGGGATGGTTCCGGCGGCGGCTGCCGGTGGCGGCTTTGAGTTTGTTGGGGGCCGACGCTTT
GGATCGGGCCATAGCTGCCGGTTGGCTGGCTCCTCTGCCGGCAAGCCTGTTGGGAGAACGATGGCAGAATCTGGATCCCC
GTTGGCAAGCTGCGGCACTCCGGGAGGGGCAGATTTGGGGTGTGCCCTGGCGCTGGGGGGTAACGGCCATTGCCTACCGC
CGGGATCGGGTGAGGGAGCCCATCCGGGACTGGGCGGATCTGTGGCGGCCTGAGCTGGCCGGCAAACTGACTCTGCCGGA
TCATCCGCGGGAGGTGATTGGGCTAACCCTAAAGAAAATGGGCCGCTCCTACAACGATCCCCTCAACCCTGAGGATGTGG
AATTACGGCAAGAGCTGGGATCCCTGCACCGCCAGGTTTTAACTTACACCTCTTCCGACTACCTGCCCATCTTGCGCTTG
GCCGATAGCTGGGTGGCCGTGGGCTGGTCGCAGGATCTCTACGCCACCCAAGCCAACTACCCTGAGCTGGCAGTGGTGAT
CCCGGCCTCCGGATCCGCCATCTGGTGGGATGTCTGGGTGCGACCCCACGTCGAAGACCATGCCCACGAAGACAATGCCC
ACACAGAATCAACCCAGACAGATTCCGGATCCCTGTTGGCTGACTGGCTTGATTTTGTCTTGAATCCTGAATTTGCCCCC
CGCTTGGTCAATCTGAGCGGGATCCCTTCGGTTTTGCCGCTGGATTTGGCTCAACTTGCCCCACGCCTGCAACGCAGAGC
CGACTTGCAAATCTCCACTTGGCAACGCTCGGAGATCTGGCGCCCCCTGAGCTCAGCCGAAGCCGAGTCCTATCTCAACC
TCTGGGATCAAATGCGGCGGGGCCTTTTGTAG

Upstream 100 bases:

>100_bases
CCACCGCAGGGATGGGAGCGGCTGTTGCCCAAGGGGATGCGGGTGCCCGCCAAGGCTTACGATGATCTGGTGGCTCTCAT
TCTGGTGGAGCGGTACTGTC

Downstream 100 bases:

>100_bases
AAACACAGCGAGCGCGAGCCCTCCACTCTGCAACACTTCGTCAAGGTCCGCAATGCTTCTGAGGAGCTGTGGTAGGATGC
CTGCTGTCTGATGAGCCAGT

Product: polyamine ABC transporter, periplasmic polyamine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MGEIRLSRRELLQGILLGLMAAGCGGPDAHLPLVVALKQGIPASLLNEFRRRTQARFRLQLLDERDQLLAYLQQSVQPAD
AAWWDPGGWFRRRLPVAALSLLGADALDRAIAAGWLAPLPASLLGERWQNLDPRWQAAALREGQIWGVPWRWGVTAIAYR
RDRVREPIRDWADLWRPELAGKLTLPDHPREVIGLTLKKMGRSYNDPLNPEDVELRQELGSLHRQVLTYTSSDYLPILRL
ADSWVAVGWSQDLYATQANYPELAVVIPASGSAIWWDVWVRPHVEDHAHEDNAHTESTQTDSGSLLADWLDFVLNPEFAP
RLVNLSGIPSVLPLDLAQLAPRLQRRADLQISTWQRSEIWRPLSSAEAESYLNLWDQMRRGLL

Sequences:

>Translated_383_residues
MGEIRLSRRELLQGILLGLMAAGCGGPDAHLPLVVALKQGIPASLLNEFRRRTQARFRLQLLDERDQLLAYLQQSVQPAD
AAWWDPGGWFRRRLPVAALSLLGADALDRAIAAGWLAPLPASLLGERWQNLDPRWQAAALREGQIWGVPWRWGVTAIAYR
RDRVREPIRDWADLWRPELAGKLTLPDHPREVIGLTLKKMGRSYNDPLNPEDVELRQELGSLHRQVLTYTSSDYLPILRL
ADSWVAVGWSQDLYATQANYPELAVVIPASGSAIWWDVWVRPHVEDHAHEDNAHTESTQTDSGSLLADWLDFVLNPEFAP
RLVNLSGIPSVLPLDLAQLAPRLQRRADLQISTWQRSEIWRPLSSAEAESYLNLWDQMRRGLL
>Mature_382_residues
GEIRLSRRELLQGILLGLMAAGCGGPDAHLPLVVALKQGIPASLLNEFRRRTQARFRLQLLDERDQLLAYLQQSVQPADA
AWWDPGGWFRRRLPVAALSLLGADALDRAIAAGWLAPLPASLLGERWQNLDPRWQAAALREGQIWGVPWRWGVTAIAYRR
DRVREPIRDWADLWRPELAGKLTLPDHPREVIGLTLKKMGRSYNDPLNPEDVELRQELGSLHRQVLTYTSSDYLPILRLA
DSWVAVGWSQDLYATQANYPELAVVIPASGSAIWWDVWVRPHVEDHAHEDNAHTESTQTDSGSLLADWLDFVLNPEFAPR
LVNLSGIPSVLPLDLAQLAPRLQRRADLQISTWQRSEIWRPLSSAEAESYLNLWDQMRRGLL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=295, Percent_Identity=25.4237288135593, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787078, Length=223, Percent_Identity=27.8026905829596, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 43420; Mature: 43289

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEIRLSRRELLQGILLGLMAAGCGGPDAHLPLVVALKQGIPASLLNEFRRRTQARFRLQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LLDERDQLLAYLQQSVQPADAAWWDPGGWFRRRLPVAALSLLGADALDRAIAAGWLAPLP
HHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASLLGERWQNLDPRWQAAALREGQIWGVPWRWGVTAIAYRRDRVREPIRDWADLWRPELA
HHHHHHHHCCCCCCHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHC
GKLTLPDHPREVIGLTLKKMGRSYNDPLNPEDVELRQELGSLHRQVLTYTSSDYLPILRL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ADSWVAVGWSQDLYATQANYPELAVVIPASGSAIWWDVWVRPHVEDHAHEDNAHTESTQT
HCCHHEECCCCCHHEECCCCCCEEEEEECCCCEEEEEEEECCCCHHCCCCCCCCCCCCCC
DSGSLLADWLDFVLNPEFAPRLVNLSGIPSVLPLDLAQLAPRLQRRADLQISTWQRSEIW
CCCHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHH
RPLSSAEAESYLNLWDQMRRGLL
CCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GEIRLSRRELLQGILLGLMAAGCGGPDAHLPLVVALKQGIPASLLNEFRRRTQARFRLQ
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LLDERDQLLAYLQQSVQPADAAWWDPGGWFRRRLPVAALSLLGADALDRAIAAGWLAPLP
HHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASLLGERWQNLDPRWQAAALREGQIWGVPWRWGVTAIAYRRDRVREPIRDWADLWRPELA
HHHHHHHHCCCCCCHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHC
GKLTLPDHPREVIGLTLKKMGRSYNDPLNPEDVELRQELGSLHRQVLTYTSSDYLPILRL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ADSWVAVGWSQDLYATQANYPELAVVIPASGSAIWWDVWVRPHVEDHAHEDNAHTESTQT
HCCHHEECCCCCHHEECCCCCCEEEEEECCCCEEEEEEEECCCCHHCCCCCCCCCCCCCC
DSGSLLADWLDFVLNPEFAPRLVNLSGIPSVLPLDLAQLAPRLQRRADLQISTWQRSEIW
CCCHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHH
RPLSSAEAESYLNLWDQMRRGLL
CCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]