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The map label for this gene is gatA

Identifier: 86609068

GI number: 86609068

Start: 1676295

End: 1677827

Strand: Direct

Name: gatA

Synonym: CYB_1605

Alternate gene names: 86609068

Gene position: 1676295-1677827 (Clockwise)

Preceding gene: 86609067

Following gene: 86609073

Centisome position: 55.02

GC content: 63.14

Gene sequence:

>1533_bases
ATGTCTGTCATCCGTGCCCTCCACAGGCAGTTGGTAACCAAAGAGCGCTCTGCCGAAGAGATCGCCCGCGAGTACCTGGA
GCGCCTGGCCCAACTGGAGCCGCAGCTCAAGAGCTTTATTACTGTGACCGAGGAACTGGCTCTGCAACAGGCCAGGGCGG
TGGACGCCCGCATTCGGGCCGGGGAGGCGATCGGCCCTTTGGCCGGGATCCCGCTTGCCGTGAAAGACAACCTCTGCACC
CAAGGGATCCGCACCACCTGCGCTTCCCGGATGCTGGAGGGGTTCATTCCCCCCTATGAATCAACGGTGACGGCGCGGCT
GGCGGCTGCGGGCATGGTGACGGTGGGCAAGACCAACCTAGACGAGTTTGCCATGGGCAGCTCCACGGAAAACTCCGCCT
TTCAGCGCACCGCCAATCCCTGGGATCTGACGCGGGTGCCAGGGGGATCCTCTGGGGGATCGGCGGCGGCGGTGGCTGCC
GACCAAGCGGTGGTGGCCCTGGGCTCAGATACGGGGGGATCCATCCGCCAACCGGCAGCTTTTTGCGGGGTGGTGGGCCT
CAAGCCCACCTACGGGCTGGTGTCTCGCTACGGGCTGGTGGCCTTTGCTTCGTCTTTGGATCAGGTGGGGCCGTTTGGGC
GCACGGTGGAGGATGTGGCCTTGCTGCTGCAGGGGATCGCCGGCCATGACCCGATGGACTCCACCAGCCTGAAGGTGGAT
ATCCCGGACTATAGCCAGGCTCTGATCCCGGACATCAAGGGGTTCAAAATCGGGGTGATCCGCGACTTGCTGGGGGAAGG
ATGTGGGGAGGAGACCCGCGCTGCCGTGCAAGCGGCCATTCAACATCTGCAGGAACTGGGGGCCGAGATCCTGGAGATCG
ACTGCCCCAGTTTTCGCTATGGCCTAGCCACCTATTACATCATCGCCCCTTCGGAAGCCTCTGCCAATTTGGCCCGCTAC
GACGGGGTGAAATACGGCCTGCGGGAACCTGCCGACTCGCTGCTGTCGATGTATGGCAAGACCCGCGCCCACGGCTTTGG
GCCAGAAGTCAAGCGGCGCATCATGATCGGCACCTATGCCCTCTCCTCGGGGTACTACGACGCCTACTACCTCAAGGCTC
AGAAGGTGCGCACCCTCATCAAGCAGGACTTCCTCAAGGCTTTCGAGAAGGTGGATGTGCTGGTCAGCCCCACCACCCCC
ACCCCAGCCTTCAAGCTCGGCGAGCGGGAGGATCCCCTCAGCATGTACCTCTGCGACCTGATGACGATCCCGGTGAACCT
GGCGGGCTTGCCGGGGCTGAGCCTGCCCTGCGGGTTTGCCGATGGCCTGCCCATTGGCCTGCAAATTGTGGGCAATGCCC
TACAGGAGAGCAAGGTGCTGCGGGTGGCCTATGCCTATGAGCAAAGCACCGACTGGCACAAGCGACGGCCGCCGCTGGGG
CAGCCCTCAGTTAAAGAGGGCCAGGGATCCGATCCCGGCCAACCTCAAGCCAAGCGCAGATCCGGCAAAAGGAGCAAAAA
ATCCAAAAGCTGA

Upstream 100 bases:

>100_bases
AAATTGGCGGGATTTTGGGTAGGAAGGGATCCCACCTAGATTAATCTAGAGGAACTTTGTCTGGCCTCACCGGCATTTGC
CCTTCCCCACGAGTTTGCCT

Downstream 100 bases:

>100_bases
GGCGGGAAGGGCGGCAACTAGGCCATGCCCATCTGGTTGGCCGCTGAAAAGCGTTCCATAAAGCGCAGGAAGCGATCCCA
CTCTGCCTCGGTTTCCATGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQARAVDARIRAGEAIGPLAGIPLAVKDNLCT
QGIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNLDEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAA
DQAVVALGSDTGGSIRQPAAFCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPMDSTSLKVD
IPDYSQALIPDIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLQELGAEILEIDCPSFRYGLATYYIIAPSEASANLARY
DGVKYGLREPADSLLSMYGKTRAHGFGPEVKRRIMIGTYALSSGYYDAYYLKAQKVRTLIKQDFLKAFEKVDVLVSPTTP
TPAFKLGEREDPLSMYLCDLMTIPVNLAGLPGLSLPCGFADGLPIGLQIVGNALQESKVLRVAYAYEQSTDWHKRRPPLG
QPSVKEGQGSDPGQPQAKRRSGKRSKKSKS

Sequences:

>Translated_510_residues
MSVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQARAVDARIRAGEAIGPLAGIPLAVKDNLCT
QGIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNLDEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAA
DQAVVALGSDTGGSIRQPAAFCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPMDSTSLKVD
IPDYSQALIPDIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLQELGAEILEIDCPSFRYGLATYYIIAPSEASANLARY
DGVKYGLREPADSLLSMYGKTRAHGFGPEVKRRIMIGTYALSSGYYDAYYLKAQKVRTLIKQDFLKAFEKVDVLVSPTTP
TPAFKLGEREDPLSMYLCDLMTIPVNLAGLPGLSLPCGFADGLPIGLQIVGNALQESKVLRVAYAYEQSTDWHKRRPPLG
QPSVKEGQGSDPGQPQAKRRSGKRSKKSKS
>Mature_509_residues
SVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQARAVDARIRAGEAIGPLAGIPLAVKDNLCTQ
GIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNLDEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAAD
QAVVALGSDTGGSIRQPAAFCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPMDSTSLKVDI
PDYSQALIPDIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLQELGAEILEIDCPSFRYGLATYYIIAPSEASANLARYD
GVKYGLREPADSLLSMYGKTRAHGFGPEVKRRIMIGTYALSSGYYDAYYLKAQKVRTLIKQDFLKAFEKVDVLVSPTTPT
PAFKLGEREDPLSMYLCDLMTIPVNLAGLPGLSLPCGFADGLPIGLQIVGNALQESKVLRVAYAYEQSTDWHKRRPPLGQ
PSVKEGQGSDPGQPQAKRRSGKRSKKSKS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=466, Percent_Identity=39.4849785407725, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI195972892, Length=478, Percent_Identity=26.3598326359833, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI166795287, Length=157, Percent_Identity=35.6687898089172, Blast_Score=73, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=439, Percent_Identity=41.6856492027335, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17537465, Length=489, Percent_Identity=24.9488752556237, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=295, Percent_Identity=30.5084745762712, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=237, Percent_Identity=28.6919831223629, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=340, Percent_Identity=27.9411764705882, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=340, Percent_Identity=27.9411764705882, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=173, Percent_Identity=30.635838150289, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=461, Percent_Identity=33.6225596529284, Blast_Score=241, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6319685, Length=448, Percent_Identity=31.25, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=236, Percent_Identity=28.8135593220339, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648113, Length=457, Percent_Identity=40.9190371991247, Blast_Score=311, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=25.7545271629779, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=25.7545271629779, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=25.7545271629779, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=25.7545271629779, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24644968, Length=501, Percent_Identity=27.5449101796407, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI161078093, Length=517, Percent_Identity=27.0793036750484, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648435, Length=238, Percent_Identity=32.7731092436975, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648437, Length=238, Percent_Identity=32.7731092436975, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45550774, Length=238, Percent_Identity=32.7731092436975, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356731, Length=481, Percent_Identity=23.0769230769231, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648441, Length=179, Percent_Identity=32.4022346368715, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648439, Length=179, Percent_Identity=32.4022346368715, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_SYNJB (Q2JL51)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477830.1
- ProteinModelPortal:   Q2JL51
- SMR:   Q2JL51
- STRING:   Q2JL51
- GeneID:   3901725
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1605
- TIGR:   CYB_1605
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q2JL51
- ProtClustDB:   PRK00012
- BioCyc:   SSP321332:CYB_1605-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 54761; Mature: 54630

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 75-75 ACT_SITE 150-150 ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQARAVDARIRA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEAIGPLAGIPLAVKDNLCTQGIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNL
CCCCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEECCCCH
DEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAADQAVVALGSDTGGSIRQPAA
HHHHCCCCCCCHHHHHCCCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHH
FCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPMDSTSLKVD
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
IPDYSQALIPDIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLQELGAEILEIDCPSFRY
CCCCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCHHH
GLATYYIIAPSEASANLARYDGVKYGLREPADSLLSMYGKTRAHGFGPEVKRRIMIGTYA
CCEEEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCHHCCCCCHHHHHEEEEEEEE
LSSGYYDAYYLKAQKVRTLIKQDFLKAFEKVDVLVSPTTPTPAFKLGEREDPLSMYLCDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
MTIPVNLAGLPGLSLPCGFADGLPIGLQIVGNALQESKVLRVAYAYEQSTDWHKRRPPLG
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEHHHCCCCHHHCCCCCC
QPSVKEGQGSDPGQPQAKRRSGKRSKKSKS
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQARAVDARIRA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEAIGPLAGIPLAVKDNLCTQGIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNL
CCCCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEECCCCH
DEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAADQAVVALGSDTGGSIRQPAA
HHHHCCCCCCCHHHHHCCCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHH
FCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPMDSTSLKVD
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
IPDYSQALIPDIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLQELGAEILEIDCPSFRY
CCCCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCHHH
GLATYYIIAPSEASANLARYDGVKYGLREPADSLLSMYGKTRAHGFGPEVKRRIMIGTYA
CCEEEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCHHCCCCCHHHHHEEEEEEEE
LSSGYYDAYYLKAQKVRTLIKQDFLKAFEKVDVLVSPTTPTPAFKLGEREDPLSMYLCDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
MTIPVNLAGLPGLSLPCGFADGLPIGLQIVGNALQESKVLRVAYAYEQSTDWHKRRPPLG
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEHHHCCCCHHHCCCCCC
QPSVKEGQGSDPGQPQAKRRSGKRSKKSKS
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA