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The map label for this gene is truA [H]

Identifier: 86609047

GI number: 86609047

Start: 1654378

End: 1655235

Strand: Direct

Name: truA [H]

Synonym: CYB_1582

Alternate gene names: 86609047

Gene position: 1654378-1655235 (Clockwise)

Preceding gene: 86609046

Following gene: 86609048

Centisome position: 54.3

GC content: 65.5

Gene sequence:

>858_bases
ATGCCGTACCGTCGCATTGCCCTGAGAATTCAGTACCTCGGCAGCAGGTTCTATGGCTGGCAGCGCCAGGCAGCCCGCCG
TACCGTGCAAGGGGTGCTGGAGGAGGCGATTGCGTCGCGGGCGGGGCATCCGGTAACCCTCTATGGGGCCGGTCGCACCG
ATACGGGGGTTCATGCTGCAGGCCAGGTGGCCCACTTCGACACCACCTCCGGCATCCCTGCGGTGGAGTGGTCGAGGGTG
CTGAACGACTGTCTGCCGGAAGATGTGGTAGTGCTGGCTTCTGCTCAAGTGCCTGACTCCTGGCACGCCCGCTTTTCGGC
TCGCTGGCGCCGCTACCGCTACCTGATCCTGAACCGGGAGCGACCGGATGTGTTCTGGCGCCTCTTTAGCTGGCAAGTGC
GTTGGCCTCTGCAGGTGGAGCCGATGGCGGAAGCCCTGAATTCCCTGCTGGGGGAGCACAGCTTGGAAGCCTTCCGGCGG
CGGGGATCGGATCGGCCCCACTCGCGGGTACAGGTGCAGGAGGTGCTCTGCCAGCGGCTGGGGGACTTGATCAGCATCGA
GGTGCAGGCCAGCGGCTTCCTCTACCGGATGATGCGGCTCTTGGTGGGATCCCTAGAGGTGGTGGGCCGGGGCCAGCTCA
GCCCAGCCCAATTTGTCCACCTGTGGCAACACAACGACTGGAGCCAGATGCGCACCCGCTACAGCGCTCCTCCGCAAGGG
CTGTGCCTGACGGATGTGGGCTATGCTGCGGATCCCTTCGGTCAACAGTTCGCCCACTCGCCGCCCTTTTGGGCCTTGCC
CACAGAGCCTCCCTCCGCCCTCCGGTCGCCGGCTACGTTGGCCCATTCCTGCGGTTGA

Upstream 100 bases:

>100_bases
GATCGGCCCGGTGGCTATACCCGCATCTTGCGCACCGTGCATCGGCGGGGGGATGGAGCAGAAATGGCGGTCATCGAGCT
GGTGTAGCGGAAAGGGATCC

Downstream 100 bases:

>100_bases
TCTGGGCGATCTCCAAGCTTGTCCTGATATGTTTCCTTGCCTGCCATGAACAAAACTCCCCTTCCCAACCCCGAAACTCT
GGCGCCCCGCTGGTATCTGG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPYRRIALRIQYLGSRFYGWQRQAARRTVQGVLEEAIASRAGHPVTLYGAGRTDTGVHAAGQVAHFDTTSGIPAVEWSRV
LNDCLPEDVVVLASAQVPDSWHARFSARWRRYRYLILNRERPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRR
RGSDRPHSRVQVQEVLCQRLGDLISIEVQASGFLYRMMRLLVGSLEVVGRGQLSPAQFVHLWQHNDWSQMRTRYSAPPQG
LCLTDVGYAADPFGQQFAHSPPFWALPTEPPSALRSPATLAHSCG

Sequences:

>Translated_285_residues
MPYRRIALRIQYLGSRFYGWQRQAARRTVQGVLEEAIASRAGHPVTLYGAGRTDTGVHAAGQVAHFDTTSGIPAVEWSRV
LNDCLPEDVVVLASAQVPDSWHARFSARWRRYRYLILNRERPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRR
RGSDRPHSRVQVQEVLCQRLGDLISIEVQASGFLYRMMRLLVGSLEVVGRGQLSPAQFVHLWQHNDWSQMRTRYSAPPQG
LCLTDVGYAADPFGQQFAHSPPFWALPTEPPSALRSPATLAHSCG
>Mature_284_residues
PYRRIALRIQYLGSRFYGWQRQAARRTVQGVLEEAIASRAGHPVTLYGAGRTDTGVHAAGQVAHFDTTSGIPAVEWSRVL
NDCLPEDVVVLASAQVPDSWHARFSARWRRYRYLILNRERPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRRR
GSDRPHSRVQVQEVLCQRLGDLISIEVQASGFLYRMMRLLVGSLEVVGRGQLSPAQFVHLWQHNDWSQMRTRYSAPPQGL
CLTDVGYAADPFGQQFAHSPPFWALPTEPPSALRSPATLAHSCG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=31.3868613138686, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1788657, Length=248, Percent_Identity=38.7096774193548, Blast_Score=157, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI71985046, Length=292, Percent_Identity=25.6849315068493, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 32342; Mature: 32211

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYRRIALRIQYLGSRFYGWQRQAARRTVQGVLEEAIASRAGHPVTLYGAGRTDTGVHAA
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
GQVAHFDTTSGIPAVEWSRVLNDCLPEDVVVLASAQVPDSWHARFSARWRRYRYLILNRE
CCEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEEECC
RPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRRRGSDRPHSRVQVQEVLCQRL
CHHHHHHHHHHEECCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GDLISIEVQASGFLYRMMRLLVGSLEVVGRGQLSPAQFVHLWQHNDWSQMRTRYSAPPQG
CCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCC
LCLTDVGYAADPFGQQFAHSPPFWALPTEPPSALRSPATLAHSCG
EEEECCCCCCCCHHHHHCCCCCCEECCCCCHHHHCCCHHHHHCCC
>Mature Secondary Structure 
PYRRIALRIQYLGSRFYGWQRQAARRTVQGVLEEAIASRAGHPVTLYGAGRTDTGVHAA
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
GQVAHFDTTSGIPAVEWSRVLNDCLPEDVVVLASAQVPDSWHARFSARWRRYRYLILNRE
CCEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEEECC
RPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRRRGSDRPHSRVQVQEVLCQRL
CHHHHHHHHHHEECCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GDLISIEVQASGFLYRMMRLLVGSLEVVGRGQLSPAQFVHLWQHNDWSQMRTRYSAPPQG
CCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCC
LCLTDVGYAADPFGQQFAHSPPFWALPTEPPSALRSPATLAHSCG
EEEECCCCCCCCHHHHHCCCCCCEECCCCCHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]