Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 86609004

GI number: 86609004

Start: 1615213

End: 1616532

Strand: Reverse

Name: dnaN [H]

Synonym: CYB_1539

Alternate gene names: 86609004

Gene position: 1616532-1615213 (Counterclockwise)

Preceding gene: 86609005

Following gene: 86608997

Centisome position: 53.06

GC content: 61.21

Gene sequence:

>1320_bases
GTGTTGCGCAGCGGCAAGGCTGCAGCTCCCTATCGTGGGGAGAGCCGCTTTAACGAAGCTGTTGACGCGCCGCTGGAGGG
TTTTAGCTTCACCTGCGAGCAAGCCGATCTCAGCCACCACCTCACTTCCGTTGGACGGGCTGTGGCCAGCCGCCCCGCCC
TGCCCGTTTTGGCCAATGTGTTGGTGGAGGCCGACGCGGAAACTCAGCGGGTTGCTTTGACCGCCTTTGACTTGAACTTG
TGCATTCGCAGCGAGTTCGAGGCTCAGGTCAGGGGATCCGGTCGCACCACCCTGCCGGCCAAGCTGCTCAGCGACATCGT
CAGCCGTCTGCCCAGCGGCCCAGTGACTCTTGACGCGAGCAGCCAAGCCCAGGAGAGTGCCCCTGTGATTCTCATGTCGG
CAGCCGGCCAGTACCAAGTGCGCAGCATGTCGGCTGAAGAGTACCCCACCCTACCGGAAATATCGCTCCGCACCGCCGAC
GCAGATGGCGATTTTGTGGGGGAGGAAAGCCCAACAGCTCAGGCCCTGGAGCTGCCCATCGAGAGCCTGTTGCAAGGGAT
CGGCCAAACCCTTTTTGCGGCCTCTGGAGATGAGACCAAGCAGGTGCTCACAGGGGTGCATGTCAAGCTCTTGGCGGGGG
ATCCCCCTTGCCTGGAGTTTGCGGCCACCGACGGGCATCGTCTGGCGATAGTGCAGGTGGAGGCCGAAGGGATCCCGGTT
GCTTCGGCCCATGGGGAAATAGGGTTGGATTTCACCATTCCCGCTCGCACTTTGCGGGAGGTGGAGCGCATCCTCGGCAC
CCAGACCGCGGCCTCTACGGTGGAATTGCGGTTTGACCGATCCCAGGTGCAGTTTGTGCTGCCTCCTCGCGCCAGCAAAA
CGCAGTGCCAACAGCGGATCACCAGCCGCCTTTTGGAGGGCCAATATCCCGACTACAACCGCCTAATTCCCAAGCAATTT
GAGCGGCAGGTAACCCTGGAGCGTCGGCCTTTGATTGAGTCGCTGGAACGGGTGGGAGTACTTGCTGCCCAAAGAAACGA
CATCGTCAAGTTCAATCTTTCTGCCGCAACCCAAACCCTGCGCATCAGCACCGAGGCGCCCGATGTGGGCAGTGGCGAGG
AATCTTTGCCGGTGCAAATGTCCGGCCCTGACCTGGAGCTGGCCTTCAATGTGCGTTACCTGCTGGATGCCCTCAGGGTG
TTTCATACGCAGCAGGTGAGCCTGGAGCTAAACGGCGCCACTCAGCCAGCTATTTGGAAGCCCCTTGGGGCGATGCGGCT
CTGTTATTTGGTCATGCCGGTGCAGCTACGGGCTACCTAG

Upstream 100 bases:

>100_bases
CAGTCGGTGTAGGATCTGCGTGTGCAGCCCTCAAGTGCCCCCCATGGTGCAAGCTCAGCGTCCTGTGGATCGAGAATCGG
AAACCGAGTCGTTCGCGCCG

Downstream 100 bases:

>100_bases
TCCTAGGGAACTAATTGAAGAACAGCCTCGGTGGCTCTTGTCCCATGTTCAGAGAGCAGGTGGATGCCGATCCAGCTCAA
ACAAAGCGGTAGGTAAACTC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MLRSGKAAAPYRGESRFNEAVDAPLEGFSFTCEQADLSHHLTSVGRAVASRPALPVLANVLVEADAETQRVALTAFDLNL
CIRSEFEAQVRGSGRTTLPAKLLSDIVSRLPSGPVTLDASSQAQESAPVILMSAAGQYQVRSMSAEEYPTLPEISLRTAD
ADGDFVGEESPTAQALELPIESLLQGIGQTLFAASGDETKQVLTGVHVKLLAGDPPCLEFAATDGHRLAIVQVEAEGIPV
ASAHGEIGLDFTIPARTLREVERILGTQTAASTVELRFDRSQVQFVLPPRASKTQCQQRITSRLLEGQYPDYNRLIPKQF
ERQVTLERRPLIESLERVGVLAAQRNDIVKFNLSAATQTLRISTEAPDVGSGEESLPVQMSGPDLELAFNVRYLLDALRV
FHTQQVSLELNGATQPAIWKPLGAMRLCYLVMPVQLRAT

Sequences:

>Translated_439_residues
MLRSGKAAAPYRGESRFNEAVDAPLEGFSFTCEQADLSHHLTSVGRAVASRPALPVLANVLVEADAETQRVALTAFDLNL
CIRSEFEAQVRGSGRTTLPAKLLSDIVSRLPSGPVTLDASSQAQESAPVILMSAAGQYQVRSMSAEEYPTLPEISLRTAD
ADGDFVGEESPTAQALELPIESLLQGIGQTLFAASGDETKQVLTGVHVKLLAGDPPCLEFAATDGHRLAIVQVEAEGIPV
ASAHGEIGLDFTIPARTLREVERILGTQTAASTVELRFDRSQVQFVLPPRASKTQCQQRITSRLLEGQYPDYNRLIPKQF
ERQVTLERRPLIESLERVGVLAAQRNDIVKFNLSAATQTLRISTEAPDVGSGEESLPVQMSGPDLELAFNVRYLLDALRV
FHTQQVSLELNGATQPAIWKPLGAMRLCYLVMPVQLRAT
>Mature_439_residues
MLRSGKAAAPYRGESRFNEAVDAPLEGFSFTCEQADLSHHLTSVGRAVASRPALPVLANVLVEADAETQRVALTAFDLNL
CIRSEFEAQVRGSGRTTLPAKLLSDIVSRLPSGPVTLDASSQAQESAPVILMSAAGQYQVRSMSAEEYPTLPEISLRTAD
ADGDFVGEESPTAQALELPIESLLQGIGQTLFAASGDETKQVLTGVHVKLLAGDPPCLEFAATDGHRLAIVQVEAEGIPV
ASAHGEIGLDFTIPARTLREVERILGTQTAASTVELRFDRSQVQFVLPPRASKTQCQQRITSRLLEGQYPDYNRLIPKQF
ERQVTLERRPLIESLERVGVLAAQRNDIVKFNLSAATQTLRISTEAPDVGSGEESLPVQMSGPDLELAFNVRYLLDALRV
FHTQQVSLELNGATQPAIWKPLGAMRLCYLVMPVQLRAT

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=414, Percent_Identity=24.8792270531401, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 47607; Mature: 47607

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSGKAAAPYRGESRFNEAVDAPLEGFSFTCEQADLSHHLTSVGRAVASRPALPVLANV
CCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHH
LVEADAETQRVALTAFDLNLCIRSEFEAQVRGSGRTTLPAKLLSDIVSRLPSGPVTLDAS
HHHCCCCCCEEEEEEEEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCEEECCC
SQAQESAPVILMSAAGQYQVRSMSAEEYPTLPEISLRTADADGDFVGEESPTAQALELPI
CCCCCCCCEEEEECCCCEEEEECCHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCH
ESLLQGIGQTLFAASGDETKQVLTGVHVKLLAGDPPCLEFAATDGHRLAIVQVEAEGIPV
HHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCEEEEEEEECCCCEE
ASAHGEIGLDFTIPARTLREVERILGTQTAASTVELRFDRSQVQFVLPPRASKTQCQQRI
EECCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHH
TSRLLEGQYPDYNRLIPKQFERQVTLERRPLIESLERVGVLAAQRNDIVKFNLSAATQTL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEE
RISTEAPDVGSGEESLPVQMSGPDLELAFNVRYLLDALRVFHTQQVSLELNGATQPAIWK
EEECCCCCCCCCCCCCCEEECCCCEEEEEEHHHHHHHHHHHHCEEEEEEECCCCCCHHHC
PLGAMRLCYLVMPVQLRAT
CHHHHHHHHHHCCEEEECC
>Mature Secondary Structure
MLRSGKAAAPYRGESRFNEAVDAPLEGFSFTCEQADLSHHLTSVGRAVASRPALPVLANV
CCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHH
LVEADAETQRVALTAFDLNLCIRSEFEAQVRGSGRTTLPAKLLSDIVSRLPSGPVTLDAS
HHHCCCCCCEEEEEEEEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCEEECCC
SQAQESAPVILMSAAGQYQVRSMSAEEYPTLPEISLRTADADGDFVGEESPTAQALELPI
CCCCCCCCEEEEECCCCEEEEECCHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCH
ESLLQGIGQTLFAASGDETKQVLTGVHVKLLAGDPPCLEFAATDGHRLAIVQVEAEGIPV
HHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCEEEEEEEECCCCEE
ASAHGEIGLDFTIPARTLREVERILGTQTAASTVELRFDRSQVQFVLPPRASKTQCQQRI
EECCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHH
TSRLLEGQYPDYNRLIPKQFERQVTLERRPLIESLERVGVLAAQRNDIVKFNLSAATQTL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEE
RISTEAPDVGSGEESLPVQMSGPDLELAFNVRYLLDALRVFHTQQVSLELNGATQPAIWK
EEECCCCCCCCCCCCCCEEECCCCEEEEEEHHHHHHHHHHHHCEEEEEEECCCCCCHHHC
PLGAMRLCYLVMPVQLRAT
CHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]