Click here to switch to the map view.

The map label for this gene is lysS [H]

Identifier: 86608981

GI number: 86608981

Start: 1587285

End: 1588820

Strand: Direct

Name: lysS [H]

Synonym: CYB_1514

Alternate gene names: 86608981

Gene position: 1587285-1588820 (Clockwise)

Preceding gene: 86608980

Following gene: 86608987

Centisome position: 52.1

GC content: 62.89

Gene sequence:

>1536_bases
ATGTCCACTCTCCCCAGCAGCAGCGAAGCCGCCCAACGGGCCGCCCGCCTGGAAAAAGCCCAACACCTGCGGGAACAAGG
GATCCCGCCCTATGCCTATTCTTTTCCTGTTACCCATCTGGCTGCCGAGCTGCAGGAGCAATTTGCCGACCTAGAGCCCG
GCGGCGAGCGGGAGGATGTGCAGGTGGCGGTGGCCGGGCGGATCCGGGCGCGGCGGGTCATGGGCAAGCTGGCTTTCTTT
ACCCTTCAGGATCGCTCCGGCACCATTCAGCTTTATCTAGACAAGAAACGGCTGACGGAGCAGATGGGGGAGCTGGCCTT
CAAGAACCTGGACAAGCTCATCGACGTTGGCGATTGGCTGGGGGCCAAGGGCTGCCTGAAGCGTACCGAAAAAGGGGAAT
TGTCGGTGCGGGTGGATGAATACCAAGTGCTGACCAAGGCCCTGCTGCCGCTGCCGGACAAGTGGCACGGCCTGCGGGAT
GTGGAGAAACGCTACCGCCAGCGCTACGTCGATCTCATCGTCAACCCTGAGGTCAAGCAGACCTTGCTCGACCGCGCCCA
GATCATCCGCTCCCTGCGGCAGACTCTGGAAGAGCGAGGTTTTGTGGAAATTGAGACGCCAGTGTTGCAGGTGATCCCCG
GCGGGGCGGAGGCGCGGCCTTTTGTTACCCACCACAACGCCCTGGATCTGGATCTCTACTTGCGCATTGCCACCGAGCTG
CACCTGAAGCGGCTGGTGGTGGGCGGGATCGAGCGAGTCTATGAAATTGGGCGGGTCTTTCGCAACGAAGGCATCTCCAC
CCGCCACAACCCGGAGTTCACCTCTCTAGAGGCCTATCAAGCCTTCGCCGACTACCACACCATGATGGAGCTGACGGAAA
CCCTGGTCTGCAACGCCGCCCTGGCGGTGCGGGGATCCCTGCAGCTCACCTACCAAGGGGAAGCCATCGACCTCACCCCT
CCCTGGCGACGGGTGACGATGCAGGAGCTGGTGGAGGAGGCCACTGGCATTTTCATCGATGTGGAAAAGGGATGCCCTGA
AGAGCTCTTGCCGGCCTTGGCCGCCCAAGGGATCCCCGATCTCTCCCCCCAGGACTCCCCCGGCCAGTTGCTGGTCAAAG
CCTTTGAGCACTGCTGCGAGAGCCGCCTGCGCCAGCCCACCTTTGTTCTGGACTATCCCCTGGAGGTGTGTCCCCTCACC
AAGCCCCACCGCAGCAAACCGGGGTTGGCCGAGCGCTTCGAGCTGTTCATCGTTGGGCGGGAGACGGCCAACGCCTACTC
CGAGCTCACCGATCCGGTGGATCAGCGGCAGCGGCTGGAGGCCCAGGCAGCCCTGAAGGCAGCCGGCGATGAAGAGGCCC
ATTTTCTGGATGAAGACTTTCTCACCGCCTTGGAATATGGCCTGCCCCCCACAGGTGGCCTGGGCGTGGGGATCGACCGC
TTGGTGATGCTGCTGTGCGACGCTCCCAGCATTCGCGATGTCATTGCCTTTCCGCTGCTGCGGCCCGAGACGGGAGAAGT
GGCGGAGGAGGCTTGA

Upstream 100 bases:

>100_bases
ACGCGAAAGGACTCCGTCCCGCTAACGCGATCGCACCGCCGGCAGCCTGAAAAATCCCGTCCCTAACGGGGCTTCCATTG
TCCATTGCCTATCCACCGCC

Downstream 100 bases:

>100_bases
GCCGATCCCTCTAACTGTCCATTCAAATCCCATCGATTTCTAAGAGAGGTTGGAGGTGGCCGCGGCGACGGCGCGGAGCC
TTTGGGAAGTGTCTCAACCT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSTLPSSSEAAQRAARLEKAQHLREQGIPPYAYSFPVTHLAAELQEQFADLEPGGEREDVQVAVAGRIRARRVMGKLAFF
TLQDRSGTIQLYLDKKRLTEQMGELAFKNLDKLIDVGDWLGAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRD
VEKRYRQRYVDLIVNPEVKQTLLDRAQIIRSLRQTLEERGFVEIETPVLQVIPGGAEARPFVTHHNALDLDLYLRIATEL
HLKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTETLVCNAALAVRGSLQLTYQGEAIDLTP
PWRRVTMQELVEEATGIFIDVEKGCPEELLPALAAQGIPDLSPQDSPGQLLVKAFEHCCESRLRQPTFVLDYPLEVCPLT
KPHRSKPGLAERFELFIVGRETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGVGIDR
LVMLLCDAPSIRDVIAFPLLRPETGEVAEEA

Sequences:

>Translated_511_residues
MSTLPSSSEAAQRAARLEKAQHLREQGIPPYAYSFPVTHLAAELQEQFADLEPGGEREDVQVAVAGRIRARRVMGKLAFF
TLQDRSGTIQLYLDKKRLTEQMGELAFKNLDKLIDVGDWLGAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRD
VEKRYRQRYVDLIVNPEVKQTLLDRAQIIRSLRQTLEERGFVEIETPVLQVIPGGAEARPFVTHHNALDLDLYLRIATEL
HLKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTETLVCNAALAVRGSLQLTYQGEAIDLTP
PWRRVTMQELVEEATGIFIDVEKGCPEELLPALAAQGIPDLSPQDSPGQLLVKAFEHCCESRLRQPTFVLDYPLEVCPLT
KPHRSKPGLAERFELFIVGRETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGVGIDR
LVMLLCDAPSIRDVIAFPLLRPETGEVAEEA
>Mature_510_residues
STLPSSSEAAQRAARLEKAQHLREQGIPPYAYSFPVTHLAAELQEQFADLEPGGEREDVQVAVAGRIRARRVMGKLAFFT
LQDRSGTIQLYLDKKRLTEQMGELAFKNLDKLIDVGDWLGAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRDV
EKRYRQRYVDLIVNPEVKQTLLDRAQIIRSLRQTLEERGFVEIETPVLQVIPGGAEARPFVTHHNALDLDLYLRIATELH
LKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTETLVCNAALAVRGSLQLTYQGEAIDLTPP
WRRVTMQELVEEATGIFIDVEKGCPEELLPALAAQGIPDLSPQDSPGQLLVKAFEHCCESRLRQPTFVLDYPLEVCPLTK
PHRSKPGLAERFELFIVGRETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGVGIDRL
VMLLCDAPSIRDVIAFPLLRPETGEVAEEA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=500, Percent_Identity=44.4, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI194272210, Length=500, Percent_Identity=44.4, Blast_Score=420, Evalue=1e-117,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=49.0945674044266, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1789256, Length=502, Percent_Identity=49.601593625498, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI87082379, Length=334, Percent_Identity=32.0359281437126, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788173, Length=251, Percent_Identity=30.2788844621514, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=503, Percent_Identity=43.7375745526839, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17535927, Length=503, Percent_Identity=43.7375745526839, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71994340, Length=503, Percent_Identity=43.7375745526839, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32566633, Length=269, Percent_Identity=25.6505576208178, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=511, Percent_Identity=40.9001956947162, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324256, Length=500, Percent_Identity=32.8, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6325153, Length=269, Percent_Identity=25.6505576208178, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24640849, Length=496, Percent_Identity=44.5564516129032, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24640851, Length=496, Percent_Identity=44.5564516129032, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24584738, Length=282, Percent_Identity=26.9503546099291, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57326; Mature: 57195

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLPSSSEAAQRAARLEKAQHLREQGIPPYAYSFPVTHLAAELQEQFADLEPGGEREDV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCE
QVAVAGRIRARRVMGKLAFFTLQDRSGTIQLYLDKKRLTEQMGELAFKNLDKLIDVGDWL
EEEEHHHHHHHHHHHHHHEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHC
GAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRDVEKRYRQRYVDLIVNPEVKQ
CCHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
TLLDRAQIIRSLRQTLEERGFVEIETPVLQVIPGGAEARPFVTHHNALDLDLYLRIATEL
HHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHH
HLKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTETLVCNAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVRGSLQLTYQGEAIDLTPPWRRVTMQELVEEATGIFIDVEKGCPEELLPALAAQGIPD
HEECCCEEEEECCCEEECCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCC
LSPQDSPGQLLVKAFEHCCESRLRQPTFVLDYPLEVCPLTKPHRSKPGLAERFELFIVGR
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCHHHEEEEEEEEC
ETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGVGIDR
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LVMLLCDAPSIRDVIAFPLLRPETGEVAEEA
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
STLPSSSEAAQRAARLEKAQHLREQGIPPYAYSFPVTHLAAELQEQFADLEPGGEREDV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCE
QVAVAGRIRARRVMGKLAFFTLQDRSGTIQLYLDKKRLTEQMGELAFKNLDKLIDVGDWL
EEEEHHHHHHHHHHHHHHEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHC
GAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRDVEKRYRQRYVDLIVNPEVKQ
CCHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
TLLDRAQIIRSLRQTLEERGFVEIETPVLQVIPGGAEARPFVTHHNALDLDLYLRIATEL
HHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHH
HLKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTETLVCNAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVRGSLQLTYQGEAIDLTPPWRRVTMQELVEEATGIFIDVEKGCPEELLPALAAQGIPD
HEECCCEEEEECCCEEECCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCC
LSPQDSPGQLLVKAFEHCCESRLRQPTFVLDYPLEVCPLTKPHRSKPGLAERFELFIVGR
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCHHHEEEEEEEEC
ETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGVGIDR
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LVMLLCDAPSIRDVIAFPLLRPETGEVAEEA
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA