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The map label for this gene is trpB

Identifier: 86608975

GI number: 86608975

Start: 1580596

End: 1581873

Strand: Direct

Name: trpB

Synonym: CYB_1508

Alternate gene names: 86608975

Gene position: 1580596-1581873 (Clockwise)

Preceding gene: 86608968

Following gene: 86608976

Centisome position: 51.88

GC content: 62.75

Gene sequence:

>1278_bases
ATGACCTCAGCTTCTTTTGCTTTTTCCCCTCGTGTTTTGGATCCCCTTGACCCGCAGGTGCGTCCGGATCCCCAGGGGCG
GTTTGGGCCGTTTGGGGGTCGGTTTGTGCCGGAAACCCTGATGAGCGCCCTAACGGAGCTGGAGGAAGCCTTTGAGCATC
ACTGGCGGGATCCCGACTTTCGCGCCGAGTTTCACAACCTGTTGCGGGATTTTGTTGGGCGGCCCAGCCCCCTCTACTTT
GCCCAGCGGCTAACCGAATACTACGGCGGCCCGCAGATCTATCTCAAACGGGAAGATCTCAACCACACCGGCGCCCACAA
GATCAACAACGCCCTTGGCCAAGTGCTGCTGGCGGTGCGCATGGGCAAGCGGCGGATCATTGCCGAGACTGGCGCCGGCC
AACATGGGGTGGCCACGGCAACCGTGTGCGCCCGCTTTGGCCTAGAATGCGTCATCTACATGGGGGCGCTGGATATGGAG
CGGCAGGCCCCCAATGTGCAGCGGATGCGCCTTTTGGGCGCTGAGGTGCGGGGAGTGGAATCGGGCACGCGCACCCTCAA
AGACGCCCTCTCCGAGGCCATCCGCGACTGGGTAACCAACGTGGAGACCACCCACTACGTCATCGGCACGGTGGCGGGGC
CCCACCCTTACCCGAAGCTGGTGCGGGCCTTTCACGATGTCATTGGCCAGGAGACGCGGCAGCAGTGCCAGGAAAAATGG
GGAGGGCTGCCGGATGTGTTGCTGGCCTGTGTGGGGGGTGGATCCAATGCGCTGGGGCTGTTCAACGAGTTTGTCCGGGA
TCCCCAGGTGCGGCTGATCGGCATCGAAGCTGCCGGAGAAGGGCTGCACACGCCCCGCCACGCCGCTACCCTCAACCGCG
GTCGCATCGGGGTCTTGCACGGGGCGATGAGCTACCTGCTGCAGGATGAAGAAGGTCAGGTTCAGGAGGCCCACTCCATC
AGCGCTGGCTTGGATTATCCTGGCGTGGGGCCAGAGCACAGCTACCTAAAAGAAATCGGGCGGGCCGAGTACTACGCGGT
TACTGACGACGAAGCGGTGGAAGCGCTGCTGCTGCTGTCGCGCCTGGAAGGGATCATCCCCGCGCTGGAGACGGCCCATG
CGCTGGCCTACCTGAAAACTTTGGCCCCACAGCAAGGACTCCGTCCCGCTAACGCGATCGGCCCTCAGCAGCGGGTGGTG
GTCAACTGTTCTGGGCGCGGCGACAAGGATCTAAACACCGTTTTCAAGTATTTGCAGCAGCGGGGACGTAGCTTTTAG

Upstream 100 bases:

>100_bases
CGCAGAAGAAGAGAGGCGAGACTGGTCATCGCCCCTTCCTTCTGTAGAATGGGAAAGCCTCTCTCTAGTTGCAGGCTACA
AGGCCCGGACGGACTCTGCC

Downstream 100 bases:

>100_bases
AGTACGGTTGTTGTGACCACCAACGGGATTCTCTTGGGTGTTCACCTGCCGAGTTCACTTCACGGTTCGATTGCTGGGTC
TTCAAGGACTCCGTCCCGCT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTSASFAFSPRVLDPLDPQVRPDPQGRFGPFGGRFVPETLMSALTELEEAFEHHWRDPDFRAEFHNLLRDFVGRPSPLYF
AQRLTEYYGGPQIYLKREDLNHTGAHKINNALGQVLLAVRMGKRRIIAETGAGQHGVATATVCARFGLECVIYMGALDME
RQAPNVQRMRLLGAEVRGVESGTRTLKDALSEAIRDWVTNVETTHYVIGTVAGPHPYPKLVRAFHDVIGQETRQQCQEKW
GGLPDVLLACVGGGSNALGLFNEFVRDPQVRLIGIEAAGEGLHTPRHAATLNRGRIGVLHGAMSYLLQDEEGQVQEAHSI
SAGLDYPGVGPEHSYLKEIGRAEYYAVTDDEAVEALLLLSRLEGIIPALETAHALAYLKTLAPQQGLRPANAIGPQQRVV
VNCSGRGDKDLNTVFKYLQQRGRSF

Sequences:

>Translated_425_residues
MTSASFAFSPRVLDPLDPQVRPDPQGRFGPFGGRFVPETLMSALTELEEAFEHHWRDPDFRAEFHNLLRDFVGRPSPLYF
AQRLTEYYGGPQIYLKREDLNHTGAHKINNALGQVLLAVRMGKRRIIAETGAGQHGVATATVCARFGLECVIYMGALDME
RQAPNVQRMRLLGAEVRGVESGTRTLKDALSEAIRDWVTNVETTHYVIGTVAGPHPYPKLVRAFHDVIGQETRQQCQEKW
GGLPDVLLACVGGGSNALGLFNEFVRDPQVRLIGIEAAGEGLHTPRHAATLNRGRIGVLHGAMSYLLQDEEGQVQEAHSI
SAGLDYPGVGPEHSYLKEIGRAEYYAVTDDEAVEALLLLSRLEGIIPALETAHALAYLKTLAPQQGLRPANAIGPQQRVV
VNCSGRGDKDLNTVFKYLQQRGRSF
>Mature_424_residues
TSASFAFSPRVLDPLDPQVRPDPQGRFGPFGGRFVPETLMSALTELEEAFEHHWRDPDFRAEFHNLLRDFVGRPSPLYFA
QRLTEYYGGPQIYLKREDLNHTGAHKINNALGQVLLAVRMGKRRIIAETGAGQHGVATATVCARFGLECVIYMGALDMER
QAPNVQRMRLLGAEVRGVESGTRTLKDALSEAIRDWVTNVETTHYVIGTVAGPHPYPKLVRAFHDVIGQETRQQCQEKWG
GLPDVLLACVGGGSNALGLFNEFVRDPQVRLIGIEAAGEGLHTPRHAATLNRGRIGVLHGAMSYLLQDEEGQVQEAHSIS
AGLDYPGVGPEHSYLKEIGRAEYYAVTDDEAVEALLLLSRLEGIIPALETAHALAYLKTLAPQQGLRPANAIGPQQRVVV
NCSGRGDKDLNTVFKYLQQRGRSF

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=395, Percent_Identity=54.4303797468354, Blast_Score=433, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321412, Length=397, Percent_Identity=56.6750629722922, Blast_Score=457, Evalue=1e-129,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_SYNJB (Q2JLD7)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477737.1
- HSSP:   P0A2K1
- ProteinModelPortal:   Q2JLD7
- SMR:   Q2JLD7
- STRING:   Q2JLD7
- GeneID:   3901507
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1508
- TIGR:   CYB_1508
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- PhylomeDB:   Q2JLD7
- ProtClustDB:   PRK04346
- BioCyc:   SSP321332:CYB_1508-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 46822; Mature: 46691

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSASFAFSPRVLDPLDPQVRPDPQGRFGPFGGRFVPETLMSALTELEEAFEHHWRDPDF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
RAEFHNLLRDFVGRPSPLYFAQRLTEYYGGPQIYLKREDLNHTGAHKINNALGQVLLAVR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
MGKRRIIAETGAGQHGVATATVCARFGLECVIYMGALDMERQAPNVQRMRLLGAEVRGVE
HCCCCEEEECCCCCCCHHHHHHHHHHCHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCH
SGTRTLKDALSEAIRDWVTNVETTHYVIGTVAGPHPYPKLVRAFHDVIGQETRQQCQEKW
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
GGLPDVLLACVGGGSNALGLFNEFVRDPQVRLIGIEAAGEGLHTPRHAATLNRGRIGVLH
CCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCHHHH
GAMSYLLQDEEGQVQEAHSISAGLDYPGVGPEHSYLKEIGRAEYYAVTDDEAVEALLLLS
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHH
RLEGIIPALETAHALAYLKTLAPQQGLRPANAIGPQQRVVVNCSGRGDKDLNTVFKYLQQ
HHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
RGRSF
HCCCC
>Mature Secondary Structure 
TSASFAFSPRVLDPLDPQVRPDPQGRFGPFGGRFVPETLMSALTELEEAFEHHWRDPDF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
RAEFHNLLRDFVGRPSPLYFAQRLTEYYGGPQIYLKREDLNHTGAHKINNALGQVLLAVR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
MGKRRIIAETGAGQHGVATATVCARFGLECVIYMGALDMERQAPNVQRMRLLGAEVRGVE
HCCCCEEEECCCCCCCHHHHHHHHHHCHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCH
SGTRTLKDALSEAIRDWVTNVETTHYVIGTVAGPHPYPKLVRAFHDVIGQETRQQCQEKW
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
GGLPDVLLACVGGGSNALGLFNEFVRDPQVRLIGIEAAGEGLHTPRHAATLNRGRIGVLH
CCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCHHHH
GAMSYLLQDEEGQVQEAHSISAGLDYPGVGPEHSYLKEIGRAEYYAVTDDEAVEALLLLS
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHH
RLEGIIPALETAHALAYLKTLAPQQGLRPANAIGPQQRVVVNCSGRGDKDLNTVFKYLQQ
HHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
RGRSF
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA