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The map label for this gene is ispE

Identifier: 86608859

GI number: 86608859

Start: 1461829

End: 1462764

Strand: Direct

Name: ispE

Synonym: CYB_1390

Alternate gene names: 86608859

Gene position: 1461829-1462764 (Clockwise)

Preceding gene: 86608858

Following gene: 86608860

Centisome position: 47.98

GC content: 61.86

Gene sequence:

>936_bases
ATGCAAGCCTGTACCCTGATTGCCCGCGCCAAGATCAACCTCTACCTGGAAATCCTCGGCTCCCGTCCTGATGGGTATTC
AGAGGTGGCGATGGTGTTGCAGAGCATTCATTTGGCGGATCGCCTGCAGTTGAAGAGCCGTCCTCACGGGATCCACCTCA
CCTGCGATCGTCCAGAAGTGCCCACAGATGCCCGCAACCTTGCCTATCAAGCCGCTGAGCTGCTGCAAAAAGAGTGTCAC
TCTGCCGCTGGCGTGGAGATTCACATCGAAAAGCACATTCCGGTGGCAGCAGGTTTGGCGGGGGGCTCGGCTGATGCAGC
GGCGGTTTTGGTGGGGTTAAACCAACTGTGGGGGCTGGGCCTGACGGTGGGAGAGTTGCAGAGTTTGGCAGCCCGTCTGG
GATCCGACATTCCCTTCTGTATCCAGGGGGGAACGCAACTGGCCACCGGGCGCGGGGAAGTTTTGGAGCCGCTGGCCGAT
TGGGAAGGGATCCCTGTGCTGCTGGCCAAGCCCAAGCACCTGGGGGTTTCGACTGCCTGGGCCTATCAAGCCTTTCGTTC
TCGCCGGGAGATCTTGGGAGCGACTGCCCCATCTGAACCCGCGCTGCCCACCTTGCCGCAGGCGTTGGCGGCTCTCAGCC
GTCAGGATCCGCCGGCGTTAGCCAGGAGTTTGCGCAATGACTTGGAACAGGTGGTTTTGCCGGAGTACGCGATTGTAGGG
GAACTGCGCCAAGCTCTTCTCTCGGCGGGGGCACTGGGATCCCTGATGTCGGGGTCGGGGCCAACGGTCTTCGGAATCAT
GCCCAGCCTGGAGCTGGCCGCCCAAGCGCGCGACACCCTGCGTCGCCAGTTTCAAGATGTGGAGTTTTGGGTGACGCAGT
TTGCCCCCACCGGGATTCTGCTGCAGCCGGATCCCTGTAGCCTCAGCCCCAGCTAG

Upstream 100 bases:

>100_bases
GAATCGGGCTGGGAGACCTCCCCGGATAAGTCTGTGTTTTGATCAGGTCTGTACAGGCTAAGGGGAGTTGAGACTTGTTG
CTGACCCAGATTTTCACCCC

Downstream 100 bases:

>100_bases
GTTGTCGGCTGGATTGGCCGGATGCGGGATCCCCACCAGCCTTCTCTATTCTTCCCTCTGGTTGGCATAGTAGGATGAGC
ACCTAAACTTAACCTTGCGT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MQACTLIARAKINLYLEILGSRPDGYSEVAMVLQSIHLADRLQLKSRPHGIHLTCDRPEVPTDARNLAYQAAELLQKECH
SAAGVEIHIEKHIPVAAGLAGGSADAAAVLVGLNQLWGLGLTVGELQSLAARLGSDIPFCIQGGTQLATGRGEVLEPLAD
WEGIPVLLAKPKHLGVSTAWAYQAFRSRREILGATAPSEPALPTLPQALAALSRQDPPALARSLRNDLEQVVLPEYAIVG
ELRQALLSAGALGSLMSGSGPTVFGIMPSLELAAQARDTLRRQFQDVEFWVTQFAPTGILLQPDPCSLSPS

Sequences:

>Translated_311_residues
MQACTLIARAKINLYLEILGSRPDGYSEVAMVLQSIHLADRLQLKSRPHGIHLTCDRPEVPTDARNLAYQAAELLQKECH
SAAGVEIHIEKHIPVAAGLAGGSADAAAVLVGLNQLWGLGLTVGELQSLAARLGSDIPFCIQGGTQLATGRGEVLEPLAD
WEGIPVLLAKPKHLGVSTAWAYQAFRSRREILGATAPSEPALPTLPQALAALSRQDPPALARSLRNDLEQVVLPEYAIVG
ELRQALLSAGALGSLMSGSGPTVFGIMPSLELAAQARDTLRRQFQDVEFWVTQFAPTGILLQPDPCSLSPS
>Mature_311_residues
MQACTLIARAKINLYLEILGSRPDGYSEVAMVLQSIHLADRLQLKSRPHGIHLTCDRPEVPTDARNLAYQAAELLQKECH
SAAGVEIHIEKHIPVAAGLAGGSADAAAVLVGLNQLWGLGLTVGELQSLAARLGSDIPFCIQGGTQLATGRGEVLEPLAD
WEGIPVLLAKPKHLGVSTAWAYQAFRSRREILGATAPSEPALPTLPQALAALSRQDPPALARSLRNDLEQVVLPEYAIVG
ELRQALLSAGALGSLMSGSGPTVFGIMPSLELAAQARDTLRRQFQDVEFWVTQFAPTGILLQPDPCSLSPS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=301, Percent_Identity=29.9003322259136, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_SYNJB (Q2JLP6)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477621.1
- ProteinModelPortal:   Q2JLP6
- SMR:   Q2JLP6
- STRING:   Q2JLP6
- GeneID:   3901257
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1390
- TIGR:   CYB_1390
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   GMGVARA
- PhylomeDB:   Q2JLP6
- ProtClustDB:   PRK02534
- BioCyc:   SSP321332:CYB_1390-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 33088; Mature: 33088

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQACTLIARAKINLYLEILGSRPDGYSEVAMVLQSIHLADRLQLKSRPHGIHLTCDRPEV
CCHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
PTDARNLAYQAAELLQKECHSAAGVEIHIEKHIPVAAGLAGGSADAAAVLVGLNQLWGLG
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCC
LTVGELQSLAARLGSDIPFCIQGGTQLATGRGEVLEPLADWEGIPVLLAKPKHLGVSTAW
CCHHHHHHHHHHHCCCCCEEECCCCEEECCCCHHHHHHHCCCCCEEEEECCCCCCCHHHH
AYQAFRSRREILGATAPSEPALPTLPQALAALSRQDPPALARSLRNDLEQVVLPEYAIVG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHH
ELRQALLSAGALGSLMSGSGPTVFGIMPSLELAAQARDTLRRQFQDVEFWVTQFAPTGIL
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LQPDPCSLSPS
ECCCCCCCCCC
>Mature Secondary Structure
MQACTLIARAKINLYLEILGSRPDGYSEVAMVLQSIHLADRLQLKSRPHGIHLTCDRPEV
CCHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
PTDARNLAYQAAELLQKECHSAAGVEIHIEKHIPVAAGLAGGSADAAAVLVGLNQLWGLG
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCC
LTVGELQSLAARLGSDIPFCIQGGTQLATGRGEVLEPLADWEGIPVLLAKPKHLGVSTAW
CCHHHHHHHHHHHCCCCCEEECCCCEEECCCCHHHHHHHCCCCCEEEEECCCCCCCHHHH
AYQAFRSRREILGATAPSEPALPTLPQALAALSRQDPPALARSLRNDLEQVVLPEYAIVG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHH
ELRQALLSAGALGSLMSGSGPTVFGIMPSLELAAQARDTLRRQFQDVEFWVTQFAPTGIL
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LQPDPCSLSPS
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA