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The map label for this gene is folC [H]

Identifier: 86608851

GI number: 86608851

Start: 1452738

End: 1454039

Strand: Reverse

Name: folC [H]

Synonym: CYB_1382

Alternate gene names: 86608851

Gene position: 1454039-1452738 (Counterclockwise)

Preceding gene: 86608852

Following gene: 86608850

Centisome position: 47.73

GC content: 60.14

Gene sequence:

>1302_bases
TTGTCCCTAACCCTGTCCCAGATCCCAGACTGGGATTGGCCGGCCTTTCTGGAGTCCCTGGGCCATTTTGGTGTGGATCT
CAGTCTGGAACGCATGCAAGTCTTGCTGCAGCGTCTAGGACAGCCCCAAGCTGGGATCCCGGCCATCCACGTAGCCGGCA
CCAACGGCAAAGGATCTGTCTGTGCCTGGGTCAGCCACGTGTTGTGGGCGGCAGGCTATCGTGTGGGCCGCTACACCTCT
CCCCATTTGGTGGACTGGCGGGAACGGATTTGGCTGAACGGGGAATACATTTCCGCCAACGACTGGAGCCAAGCGCTGGC
CCAGGTGCGAGACTGCCTACAAACCTATGCCCCTGACGAGGCTTCTCCCACCCAGTTTGAGGTTATCACCGCTGCCGCTT
GGCTGTACTTCCGCCGGCAAAAGGTGGATGTGGCAGTGATAGAAGTGGGCCTGGGGGGGCGGCTGGATGCTACCAATGCT
GGGATCGAGCCGGTGGTGACGGCCATCACCTCCATCGGCTGGGATCATTGGCAGCGCTTGGGCAACAGCCTTCCCCAAAT
TGCCTCTGAAAAGGCGGGCATTCTCAAGCCAGGGATCCCGGCAGTGTGCGCACCCCAAACCCCAGAGGTCACAGCAGTTA
TCCGAGCCAAGGCAGAGCAATTGCGAAGCCCCCTGAGGGTGGTCGACCCTCTACAATGGTCAGAGCCTCAAACCGTCCAC
TGGCGAGGGCAGCTCCTCACCTTGCCCCTCAGCGGCGATGTACAACTCACCAATGCAGCAGTAGCCCTTGCCGTTGTGGA
AACCCTACAGCAACAGGGCTGGCGGATCGCGGATGCCGCTGTCGAAAAAGGATTTGCCATCACCCGTTGGCCGGGGCGCT
TACAGCGGGTGCAGATTGGATCCCGTCAGTTGCTGATGGACGGAGCCCACAACTTGCCCGCTGCCCAGGCTCTCCGTCGC
TATCTGGATGAGCATCAACCCGGCCCCCTCACCTGGTATGTGGGCATCCTCACCACCAAAGATGTCCAGGGCATTCTCAG
CACCCTGCTGCGGCCGGGAGATTGCTTTTTTGCCCTGCCAATTGCCGGCCACTCTGGGACGGATCCCGAGCAACTTGCTC
AGCTAGCCCAGACTCTGCAGCCTCATCTCGCCTCGGCACGGGAACTGCCCAATCTCTCCGCTCTTCGGGCTCAGTTGCTC
AAGCCGAAAAACCTCACTGAGCTACCAGCTGTAATCTGCGGATCCCTGTACCTATTGGGCCAGGTGATGCAGGAATGTTT
GGGATGGGATCTAGCCTGTTGA

Upstream 100 bases:

>100_bases
GACGCTTTTGTAGTGAACCTCTAGAATTGTTGATCCCTAGAAAAAATCCCAAATCTCACCTACCCTGGCAAGACGGAGTC
TTTGCTTGAACCAGAGCAAC

Downstream 100 bases:

>100_bases
ACTGACAGGTGAAACCTTATGCAAAACCTGAGCTCCTCTCTTATAAAGAGAGAGGAGCTATGAACAAAAACTAGCTCTCA
ACCACTATCACTTGATCAAG

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSLTLSQIPDWDWPAFLESLGHFGVDLSLERMQVLLQRLGQPQAGIPAIHVAGTNGKGSVCAWVSHVLWAAGYRVGRYTS
PHLVDWRERIWLNGEYISANDWSQALAQVRDCLQTYAPDEASPTQFEVITAAAWLYFRRQKVDVAVIEVGLGGRLDATNA
GIEPVVTAITSIGWDHWQRLGNSLPQIASEKAGILKPGIPAVCAPQTPEVTAVIRAKAEQLRSPLRVVDPLQWSEPQTVH
WRGQLLTLPLSGDVQLTNAAVALAVVETLQQQGWRIADAAVEKGFAITRWPGRLQRVQIGSRQLLMDGAHNLPAAQALRR
YLDEHQPGPLTWYVGILTTKDVQGILSTLLRPGDCFFALPIAGHSGTDPEQLAQLAQTLQPHLASARELPNLSALRAQLL
KPKNLTELPAVICGSLYLLGQVMQECLGWDLAC

Sequences:

>Translated_433_residues
MSLTLSQIPDWDWPAFLESLGHFGVDLSLERMQVLLQRLGQPQAGIPAIHVAGTNGKGSVCAWVSHVLWAAGYRVGRYTS
PHLVDWRERIWLNGEYISANDWSQALAQVRDCLQTYAPDEASPTQFEVITAAAWLYFRRQKVDVAVIEVGLGGRLDATNA
GIEPVVTAITSIGWDHWQRLGNSLPQIASEKAGILKPGIPAVCAPQTPEVTAVIRAKAEQLRSPLRVVDPLQWSEPQTVH
WRGQLLTLPLSGDVQLTNAAVALAVVETLQQQGWRIADAAVEKGFAITRWPGRLQRVQIGSRQLLMDGAHNLPAAQALRR
YLDEHQPGPLTWYVGILTTKDVQGILSTLLRPGDCFFALPIAGHSGTDPEQLAQLAQTLQPHLASARELPNLSALRAQLL
KPKNLTELPAVICGSLYLLGQVMQECLGWDLAC
>Mature_432_residues
SLTLSQIPDWDWPAFLESLGHFGVDLSLERMQVLLQRLGQPQAGIPAIHVAGTNGKGSVCAWVSHVLWAAGYRVGRYTSP
HLVDWRERIWLNGEYISANDWSQALAQVRDCLQTYAPDEASPTQFEVITAAAWLYFRRQKVDVAVIEVGLGGRLDATNAG
IEPVVTAITSIGWDHWQRLGNSLPQIASEKAGILKPGIPAVCAPQTPEVTAVIRAKAEQLRSPLRVVDPLQWSEPQTVHW
RGQLLTLPLSGDVQLTNAAVALAVVETLQQQGWRIADAAVEKGFAITRWPGRLQRVQIGSRQLLMDGAHNLPAAQALRRY
LDEHQPGPLTWYVGILTTKDVQGILSTLLRPGDCFFALPIAGHSGTDPEQLAQLAQTLQPHLASARELPNLSALRAQLLK
PKNLTELPAVICGSLYLLGQVMQECLGWDLAC

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=324, Percent_Identity=35.1851851851852, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI66932984, Length=324, Percent_Identity=35.1851851851852, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1788654, Length=424, Percent_Identity=32.7830188679245, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17553148, Length=284, Percent_Identity=33.4507042253521, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17553150, Length=291, Percent_Identity=32.9896907216495, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71984923, Length=284, Percent_Identity=33.4507042253521, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6323760, Length=423, Percent_Identity=34.7517730496454, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6324815, Length=286, Percent_Identity=32.1678321678322, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322718, Length=432, Percent_Identity=27.0833333333333, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24641571, Length=319, Percent_Identity=30.4075235109718, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24581568, Length=331, Percent_Identity=28.3987915407855, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47457; Mature: 47326

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTLSQIPDWDWPAFLESLGHFGVDLSLERMQVLLQRLGQPQAGIPAIHVAGTNGKGSV
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHH
CAWVSHVLWAAGYRVGRYTSPHLVDWRERIWLNGEYISANDWSQALAQVRDCLQTYAPDE
HHHHHHHHHHHCCCCCCCCCCCHHCHHHHEEECCCEECCCHHHHHHHHHHHHHHHHCCCC
ASPTQFEVITAAAWLYFRRQKVDVAVIEVGLGGRLDATNAGIEPVVTAITSIGWDHWQRL
CCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
GNSLPQIASEKAGILKPGIPAVCAPQTPEVTAVIRAKAEQLRSPLRVVDPLQWSEPQTVH
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEE
WRGQLLTLPLSGDVQLTNAAVALAVVETLQQQGWRIADAAVEKGFAITRWPGRLQRVQIG
ECCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCEEHHHHHHCCCEEEECCCCHHHEECC
SRQLLMDGAHNLPAAQALRRYLDEHQPGPLTWYVGILTTKDVQGILSTLLRPGDCFFALP
CHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHCCCCCEEEEEE
IAGHSGTDPEQLAQLAQTLQPHLASARELPNLSALRAQLLKPKNLTELPAVICGSLYLLG
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
QVMQECLGWDLAC
HHHHHHHCCCCCC
>Mature Secondary Structure 
SLTLSQIPDWDWPAFLESLGHFGVDLSLERMQVLLQRLGQPQAGIPAIHVAGTNGKGSV
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHH
CAWVSHVLWAAGYRVGRYTSPHLVDWRERIWLNGEYISANDWSQALAQVRDCLQTYAPDE
HHHHHHHHHHHCCCCCCCCCCCHHCHHHHEEECCCEECCCHHHHHHHHHHHHHHHHCCCC
ASPTQFEVITAAAWLYFRRQKVDVAVIEVGLGGRLDATNAGIEPVVTAITSIGWDHWQRL
CCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
GNSLPQIASEKAGILKPGIPAVCAPQTPEVTAVIRAKAEQLRSPLRVVDPLQWSEPQTVH
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEE
WRGQLLTLPLSGDVQLTNAAVALAVVETLQQQGWRIADAAVEKGFAITRWPGRLQRVQIG
ECCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCEEHHHHHHCCCEEEECCCCHHHEECC
SRQLLMDGAHNLPAAQALRRYLDEHQPGPLTWYVGILTTKDVQGILSTLLRPGDCFFALP
CHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHCCCCCEEEEEE
IAGHSGTDPEQLAQLAQTLQPHLASARELPNLSALRAQLLKPKNLTELPAVICGSLYLLG
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
QVMQECLGWDLAC
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]