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The map label for this gene is polA [H]

Identifier: 86608835

GI number: 86608835

Start: 1432669

End: 1433301

Strand: Reverse

Name: polA [H]

Synonym: CYB_1361

Alternate gene names: 86608835

Gene position: 1433301-1432669 (Counterclockwise)

Preceding gene: 86608836

Following gene: 86608834

Centisome position: 47.04

GC content: 58.77

Gene sequence:

>633_bases
GTGGGCCTGCAACCTTTCCAGCTCTGCGACGAAGATCTCACCCCTGCAGTGGCAAAGGCCCTGGCCAGGGAACCTTACCT
GGCCGTGGATACAGAAACAATGGGGCTGATCCCGCAGCGGGACAGGCTTTGCGTGGTGCAAATCGCCAACTCCGCCGGTG
AGGTGGTGCTGTTGCGGCTGAGCCGCGGGGTAAAGCAGGCCCCCTTTTTGGCGCAGCTGCTGATGGCTCCAAGCATTGAG
AAGATCTTCCACTACGCCCGCTTTGACCTGGCCATGCTGCGCTATCACCTGGGGATCCGGGCGTGGCCCGTCTTTTGCAC
CAAAATTGCCAGCAAACTGGCCCGCACCTACACCAGCAAGCATGGCCTCAAGGATGTGGTGAGCGAGCTGTGCGGGGTCG
AACTCAACAAAACTGCCCAAAGCTCCGATTGGGGAGCCGTCCACGCGCTATCCGCCGAACAGCTGGAATATGCCGCCAAC
GATGTTCGCTATTTGATCCCGGTGCGCCACAAGTTGGCGGAGATGTTACGGCGGGAAGAACGCTGGGAGTTGGCGCAGCA
GTGTTTCCAACATTTGCCTACTTTGGTAGAGCTGGATCTGTTGGGCTTCAGTAACGTCTTCGAACATCAGTAG

Upstream 100 bases:

>100_bases
CCACCGAGGCTTACTGCCAGGCGTAGTTAAGTCTAGCTGGTAGCTACCCGACCTTCGCCGCTTGGTTGAACGGCCAAAAC
ATCCTTTTGAGAGATCCTGA

Downstream 100 bases:

>100_bases
GCGGGGCTTTACTTGATAGACAGTCACTGTCTTCGATGACCGGGCCCTCGGGGATCCCACATCTCCTGAGGCTGAACATG
GGGGGACCTCACCGGCGCAT

Product: 3'-5' exonuclease family protein

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MGLQPFQLCDEDLTPAVAKALAREPYLAVDTETMGLIPQRDRLCVVQIANSAGEVVLLRLSRGVKQAPFLAQLLMAPSIE
KIFHYARFDLAMLRYHLGIRAWPVFCTKIASKLARTYTSKHGLKDVVSELCGVELNKTAQSSDWGAVHALSAEQLEYAAN
DVRYLIPVRHKLAEMLRREERWELAQQCFQHLPTLVELDLLGFSNVFEHQ

Sequences:

>Translated_210_residues
MGLQPFQLCDEDLTPAVAKALAREPYLAVDTETMGLIPQRDRLCVVQIANSAGEVVLLRLSRGVKQAPFLAQLLMAPSIE
KIFHYARFDLAMLRYHLGIRAWPVFCTKIASKLARTYTSKHGLKDVVSELCGVELNKTAQSSDWGAVHALSAEQLEYAAN
DVRYLIPVRHKLAEMLRREERWELAQQCFQHLPTLVELDLLGFSNVFEHQ
>Mature_209_residues
GLQPFQLCDEDLTPAVAKALAREPYLAVDTETMGLIPQRDRLCVVQIANSAGEVVLLRLSRGVKQAPFLAQLLMAPSIEK
IFHYARFDLAMLRYHLGIRAWPVFCTKIASKLARTYTSKHGLKDVVSELCGVELNKTAQSSDWGAVHALSAEQLEYAAND
VRYLIPVRHKLAEMLRREERWELAQQCFQHLPTLVELDLLGFSNVFEHQ

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=165, Percent_Identity=30.3030303030303, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 23760; Mature: 23629

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLQPFQLCDEDLTPAVAKALAREPYLAVDTETMGLIPQRDRLCVVQIANSAGEVVLLRL
CCCCHHHHCCCCCCHHHHHHHHCCCCEEECCHHHCCCCCCCCEEEEEECCCCCCEEEEEH
SRGVKQAPFLAQLLMAPSIEKIFHYARFDLAMLRYHLGIRAWPVFCTKIASKLARTYTSK
HCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHC
HGLKDVVSELCGVELNKTAQSSDWGAVHALSAEQLEYAANDVRYLIPVRHKLAEMLRREE
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWELAQQCFQHLPTLVELDLLGFSNVFEHQ
HHHHHHHHHHHCCHHHHHHHHCHHHHHCCC
>Mature Secondary Structure 
GLQPFQLCDEDLTPAVAKALAREPYLAVDTETMGLIPQRDRLCVVQIANSAGEVVLLRL
CCCHHHHCCCCCCHHHHHHHHCCCCEEECCHHHCCCCCCCCEEEEEECCCCCCEEEEEH
SRGVKQAPFLAQLLMAPSIEKIFHYARFDLAMLRYHLGIRAWPVFCTKIASKLARTYTSK
HCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHC
HGLKDVVSELCGVELNKTAQSSDWGAVHALSAEQLEYAANDVRYLIPVRHKLAEMLRREE
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWELAQQCFQHLPTLVELDLLGFSNVFEHQ
HHHHHHHHHHHCCHHHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]