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The map label for this gene is ligA

Identifier: 86608809

GI number: 86608809

Start: 1397455

End: 1399515

Strand: Direct

Name: ligA

Synonym: CYB_1335

Alternate gene names: 86608809

Gene position: 1397455-1399515 (Clockwise)

Preceding gene: 86608808

Following gene: 86608810

Centisome position: 45.87

GC content: 62.98

Gene sequence:

>2061_bases
ATGGATCCCAAGTTGGAAGAACGCGCCCGCGAGCTGCGCACCCTGCTGCAGAAGGCCAGCATTGCCTACTATGTCCACGA
TGCTCCCATCCTGGAAGACAGCGTTTACGACCGCCTCTACCGCGAGCTGCAAGAGCTGGAGCGGGCTTACCCTGAACTGG
TCACCCCCGATAGCCCTACTCAGCGGGTGGGAGAAAAACCTGCAGAGCATTTTCCCACCGTCTTCCACCGCATCCCCCTC
TACAGCTTGGAAAACGCTTTCAACCCAGAAGAGCTAGAGGAGTGGCAAGAGCGTCTGCTGCGAGTTTTGGGCCGTGCCCC
TGTTCCCGACAGCAGGACAGAGGGAGAGCTGGAATATGTATGTGAGCTGAAGATTGATGGCGCCGCCTTGGCCCTAACCT
ACATCGAGGGTCTTTTGGAGCGGGGTGCTACTCGGGGGGACGGACAGGCCGGAGAGGACATCACCCCCAACGTGCGCGCC
ATTCGTTCGATTCCCCTGCGGCTGGCGACAGCAGATCCGCCTCCCGTTTTGGAGGTGCGGGGGGAAGTCTATCTGGCCCT
AGCCGAGTTTGAACGGATCAACCAAGAGCGTCGTTCCGCTGGGGATCCCCCCTTTGCCAACCCGCGCAACTGTGCTGCCG
GTACCCTGCGGCAGTTGGATAGCCGCGTTGTGGCCGCCCGCAAGCTCAGCTTCTTCGCCTACGCCCTCCACTGGCCGGAG
GGATGGCCTGCCGGGGATCCCCCGCAAACCCAGTGGCAGTGTCTGCAGCAGCTCAAGGAATTGGGATTTCCGGTCAACCC
CCTTTCCCAGGTCTGTCGGGGCTTGGGAGAAGTGATCCAATTTTATGAGCGATGGCGGGGTGCCCAGCTCCCCTACGCCA
GCGACGGGGTGGTGGTGAAATTGAACTCCTTGCGGCTGCAGGAGGAGGCCGGCTTTACGCAAAAATTTCCCCGCTGGGCC
ATTGCCCTCAAATACCCAGCTCAAGAGGTTCCCACTCGCATCCGCGCCATCGTGGCCAGCGTGGGCCGTACCGGCGCCGT
CACTCCAGTGGCAGAGCTGGAGCCGGTTCCTCTGGCCGGCACCCTTGTCAGCCGCGCCAGCCTGCACAATGCCGATCGCC
TGCGGGAGCTGGACGTGCACATCGGCGATACTGCCATCGTGCGCAAAGCCGGGGAGATCATCCCGGAGGTGGTCGGGATC
CTGAAGGAATTGCGCCCTGCCGATGCCGTTCCCTACAGCCTGCCCAGCCAGTGCCCCGAATGCCACACCCCCTTGGTTCG
TCCCCCCGGCGAAGCCGTGACCCGCTGCCCCAACCCCGAGTGCCCGGCCAAGCTGCGGGGCCAATTGCAGCACTGGGCCA
GCCGCGATGCTTTGGATATCGAAGGCTTAGGGGAAAAGCGGGCAGCCCAATTGGTGGAGAGCTTGGGGGTGCGCACCGTA
GCCGATCTCTACCGCCTCACCCCAGAGCAGTTGCAGGGTTTGGAGGGAATGGGATCCCGCTCCAGCCAAAAGCTGGTGCA
AGCCATTCAGCGCTCTCGTCAACAGCCCTGGGAACGAGTGCTCTACGGGCTGGGGATCCCCCATGTGGGGGTGGTCACTG
CCCAGATTTTGGCCGGCCATTTTCCCACCCCTGAGCTTTTGGCCCAGGCCAGTGCAGAAACCATCGCCCAGATCTATGGG
ATAGGCGCGGAAGTGGCGGAAGCGGTGGTGGCCTGGTTTGCCGAGCCGGCCCATCGTCGCCTGTTGCAGGAGTTGCAGGC
GCTAGGGATCCCCGCTCTTCCTCAGCAGGAGAACCCTGCCGTCTCCCAGATCCTGGCCGGGAAAAAATTCGTAATCACCG
GCACCTTGCCCACCCTCTCCCGCCAGCAGGCCAAGGCTTGGATTGAGAGTCGGGGGGGCAAAGTCACCGCCAGCGTCAGC
CGCCAAACCGACTACGTGGTGGTGGGCTCTGACCCCGGCTCCAAGCTGGAGCAAGCCCAACAACTGGGGATCCCCCTGCT
GACCGAAGCGGAGCTGTTGGCGCTGGATCCCACCGTGGAATCCGGTGATCTGCATCCTTGA

Upstream 100 bases:

>100_bases
TCCCCTTCCTACATCCTCCCAGCTTGGAGAATATCGGCTCCTACTTGTTCGCTCCTCAGGAGCCCTAACCTAGGGGTAGA
CCTTGCTCACCCAAAGCGTT

Downstream 100 bases:

>100_bases
CAGGGTGGATCCCCGAAAAACTTGGGTAGGGTGGGTTGTCTTAGCGGCAGGGATGATTTATGATTCAACCTTAGTGAGGA
GTGGACGCCAATAGGGGGAA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MDPKLEERARELRTLLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELVTPDSPTQRVGEKPAEHFPTVFHRIPL
YSLENAFNPEELEEWQERLLRVLGRAPVPDSRTEGELEYVCELKIDGAALALTYIEGLLERGATRGDGQAGEDITPNVRA
IRSIPLRLATADPPPVLEVRGEVYLALAEFERINQERRSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPE
GWPAGDPPQTQWQCLQQLKELGFPVNPLSQVCRGLGEVIQFYERWRGAQLPYASDGVVVKLNSLRLQEEAGFTQKFPRWA
IALKYPAQEVPTRIRAIVASVGRTGAVTPVAELEPVPLAGTLVSRASLHNADRLRELDVHIGDTAIVRKAGEIIPEVVGI
LKELRPADAVPYSLPSQCPECHTPLVRPPGEAVTRCPNPECPAKLRGQLQHWASRDALDIEGLGEKRAAQLVESLGVRTV
ADLYRLTPEQLQGLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAGHFPTPELLAQASAETIAQIYG
IGAEVAEAVVAWFAEPAHRRLLQELQALGIPALPQQENPAVSQILAGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVS
RQTDYVVVGSDPGSKLEQAQQLGIPLLTEAELLALDPTVESGDLHP

Sequences:

>Translated_686_residues
MDPKLEERARELRTLLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELVTPDSPTQRVGEKPAEHFPTVFHRIPL
YSLENAFNPEELEEWQERLLRVLGRAPVPDSRTEGELEYVCELKIDGAALALTYIEGLLERGATRGDGQAGEDITPNVRA
IRSIPLRLATADPPPVLEVRGEVYLALAEFERINQERRSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPE
GWPAGDPPQTQWQCLQQLKELGFPVNPLSQVCRGLGEVIQFYERWRGAQLPYASDGVVVKLNSLRLQEEAGFTQKFPRWA
IALKYPAQEVPTRIRAIVASVGRTGAVTPVAELEPVPLAGTLVSRASLHNADRLRELDVHIGDTAIVRKAGEIIPEVVGI
LKELRPADAVPYSLPSQCPECHTPLVRPPGEAVTRCPNPECPAKLRGQLQHWASRDALDIEGLGEKRAAQLVESLGVRTV
ADLYRLTPEQLQGLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAGHFPTPELLAQASAETIAQIYG
IGAEVAEAVVAWFAEPAHRRLLQELQALGIPALPQQENPAVSQILAGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVS
RQTDYVVVGSDPGSKLEQAQQLGIPLLTEAELLALDPTVESGDLHP
>Mature_686_residues
MDPKLEERARELRTLLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELVTPDSPTQRVGEKPAEHFPTVFHRIPL
YSLENAFNPEELEEWQERLLRVLGRAPVPDSRTEGELEYVCELKIDGAALALTYIEGLLERGATRGDGQAGEDITPNVRA
IRSIPLRLATADPPPVLEVRGEVYLALAEFERINQERRSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPE
GWPAGDPPQTQWQCLQQLKELGFPVNPLSQVCRGLGEVIQFYERWRGAQLPYASDGVVVKLNSLRLQEEAGFTQKFPRWA
IALKYPAQEVPTRIRAIVASVGRTGAVTPVAELEPVPLAGTLVSRASLHNADRLRELDVHIGDTAIVRKAGEIIPEVVGI
LKELRPADAVPYSLPSQCPECHTPLVRPPGEAVTRCPNPECPAKLRGQLQHWASRDALDIEGLGEKRAAQLVESLGVRTV
ADLYRLTPEQLQGLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAGHFPTPELLAQASAETIAQIYG
IGAEVAEAVVAWFAEPAHRRLLQELQALGIPALPQQENPAVSQILAGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVS
RQTDYVVVGSDPGSKLEQAQQLGIPLLTEAELLALDPTVESGDLHP

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=676, Percent_Identity=45.1183431952663, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI87082305, Length=597, Percent_Identity=24.2881072026801, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SYNJB (Q2JLU3)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477571.1
- ProteinModelPortal:   Q2JLU3
- SMR:   Q2JLU3
- STRING:   Q2JLU3
- GeneID:   3901165
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1335
- TIGR:   CYB_1335
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   HNQDYID
- PhylomeDB:   Q2JLU3
- ProtClustDB:   CLSK750245
- BioCyc:   SSP321332:CYB_1335-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75568; Mature: 75568

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 124-124 BINDING 122-122 BINDING 145-145 BINDING 182-182 BINDING 300-300 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPKLEERARELRTLLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELVTPDSPT
CCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
QRVGEKPAEHFPTVFHRIPLYSLENAFNPEELEEWQERLLRVLGRAPVPDSRTEGELEYV
HHHCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
CELKIDGAALALTYIEGLLERGATRGDGQAGEDITPNVRAIRSIPLRLATADPPPVLEVR
EEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCCEEEC
GEVYLALAEFERINQERRSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPE
CCEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GWPAGDPPQTQWQCLQQLKELGFPVNPLSQVCRGLGEVIQFYERWRGAQLPYASDGVVVK
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LNSLRLQEEAGFTQKFPRWAIALKYPAQEVPTRIRAIVASVGRTGAVTPVAELEPVPLAG
ECCHHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHH
TLVSRASLHNADRLRELDVHIGDTAIVRKAGEIIPEVVGILKELRPADAVPYSLPSQCPE
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
CHTPLVRPPGEAVTRCPNPECPAKLRGQLQHWASRDALDIEGLGEKRAAQLVESLGVRTV
HCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHH
ADLYRLTPEQLQGLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAGH
HHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHCC
FPTPELLAQASAETIAQIYGIGAEVAEAVVAWFAEPAHRRLLQELQALGIPALPQQENPA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCHH
VSQILAGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVSRQTDYVVVGSDPGSKLEQAQ
HHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHH
QLGIPLLTEAELLALDPTVESGDLHP
HCCCCEECCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MDPKLEERARELRTLLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELVTPDSPT
CCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
QRVGEKPAEHFPTVFHRIPLYSLENAFNPEELEEWQERLLRVLGRAPVPDSRTEGELEYV
HHHCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
CELKIDGAALALTYIEGLLERGATRGDGQAGEDITPNVRAIRSIPLRLATADPPPVLEVR
EEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCCEEEC
GEVYLALAEFERINQERRSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPE
CCEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GWPAGDPPQTQWQCLQQLKELGFPVNPLSQVCRGLGEVIQFYERWRGAQLPYASDGVVVK
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LNSLRLQEEAGFTQKFPRWAIALKYPAQEVPTRIRAIVASVGRTGAVTPVAELEPVPLAG
ECCHHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHH
TLVSRASLHNADRLRELDVHIGDTAIVRKAGEIIPEVVGILKELRPADAVPYSLPSQCPE
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
CHTPLVRPPGEAVTRCPNPECPAKLRGQLQHWASRDALDIEGLGEKRAAQLVESLGVRTV
HCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHH
ADLYRLTPEQLQGLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAGH
HHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHCC
FPTPELLAQASAETIAQIYGIGAEVAEAVVAWFAEPAHRRLLQELQALGIPALPQQENPA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCHH
VSQILAGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVSRQTDYVVVGSDPGSKLEQAQ
HHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHH
QLGIPLLTEAELLALDPTVESGDLHP
HCCCCEECCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA