Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 86608693

GI number: 86608693

Start: 1262530

End: 1263285

Strand: Direct

Name: trmD [H]

Synonym: CYB_1217

Alternate gene names: 86608693

Gene position: 1262530-1263285 (Clockwise)

Preceding gene: 86608692

Following gene: 86608694

Centisome position: 41.44

GC content: 61.77

Gene sequence:

>756_bases
GTGCGGTTCGATATCCTTACCCTTTTCCCCGAATTTTTTGAAACGCCGCTGCGCATTGGGCTGGTGGGCAAAGCCCTACA
GCAGGGGATCGCCGAAGTCCACTGCACCAATCCACGCGACTTCGCCACCGATAAGCACCGCCGTGTCGATGATGAGCCCT
ACGGGGGTGGGGTGGGCATGGTGCTCAAGCCAGAGCCTTTCTTCGCTGCTGTCGCCTCCCTGCCCCGGTTGGATCCCTGC
GAGATCATTCTGCTGACGCCCCAGGGGCAACCGCTGAACCAGGCGCTGCTGCAGGAGCTGGCCCAGAAAGCGCAACTGAT
CCTGCTGTGTGGCCAGTACGAAGGCTTTGACGAACGCATCCGCCAATACCTGGCCACCCGCGAGGTGTCGCTGGGGGATT
TTGTCCTGACCGGGGGGGAAATTCCGGCGCTGGCCCTGATCAACGGCGTGGTGCGGCTCTTGCCGGGCACTGTGGGCAAG
GTAGACTCGTTAAAGAGCGAAAGCTTCCAGGCGGGGCTGTTGGACTATCCTCAATACACCCGCCCGGCAGAGTTTCAGGG
GCACAAGGTGCCGCCGGTGCTCCTCTCAGGCGACCATCAAGCCATTGCCCGTTGGCGTCTGCAGCAGCAGTTGGTTCGAA
CCTGGCAAAGGCGGCCAGACCTGTTGGCCAAGCGCCCCTTGACCGCCGAAGAGCAACAGTTGTTGGCAGAGGGGTTGGCG
GAGCAGCACCCTGGCGTGAACGATGTGGAGAAATAA

Upstream 100 bases:

>100_bases
CTATCTCCAGAGAAGAGATGGCTGAGTTGATGGAGCTGATCCACCAGATCGAGTCCTTGCGCCTGGATTTGGAGCGGTTG
CGCACCTCGTCCTGAGTCCT

Downstream 100 bases:

>100_bases
ATCATGCTCGGCAAGCTTCTCAACCGTCTCCTGAATCCATCGCCCGTCTGCTGGCGTGGGATCCCCTCGCCCCCAGTGCT
GCTTCTGGTGGGAATGGCCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRFDILTLFPEFFETPLRIGLVGKALQQGIAEVHCTNPRDFATDKHRRVDDEPYGGGVGMVLKPEPFFAAVASLPRLDPC
EIILLTPQGQPLNQALLQELAQKAQLILLCGQYEGFDERIRQYLATREVSLGDFVLTGGEIPALALINGVVRLLPGTVGK
VDSLKSESFQAGLLDYPQYTRPAEFQGHKVPPVLLSGDHQAIARWRLQQQLVRTWQRRPDLLAKRPLTAEEQQLLAEGLA
EQHPGVNDVEK

Sequences:

>Translated_251_residues
MRFDILTLFPEFFETPLRIGLVGKALQQGIAEVHCTNPRDFATDKHRRVDDEPYGGGVGMVLKPEPFFAAVASLPRLDPC
EIILLTPQGQPLNQALLQELAQKAQLILLCGQYEGFDERIRQYLATREVSLGDFVLTGGEIPALALINGVVRLLPGTVGK
VDSLKSESFQAGLLDYPQYTRPAEFQGHKVPPVLLSGDHQAIARWRLQQQLVRTWQRRPDLLAKRPLTAEEQQLLAEGLA
EQHPGVNDVEK
>Mature_251_residues
MRFDILTLFPEFFETPLRIGLVGKALQQGIAEVHCTNPRDFATDKHRRVDDEPYGGGVGMVLKPEPFFAAVASLPRLDPC
EIILLTPQGQPLNQALLQELAQKAQLILLCGQYEGFDERIRQYLATREVSLGDFVLTGGEIPALALINGVVRLLPGTVGK
VDSLKSESFQAGLLDYPQYTRPAEFQGHKVPPVLLSGDHQAIARWRLQQQLVRTWQRRPDLLAKRPLTAEEQQLLAEGLA
EQHPGVNDVEK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=239, Percent_Identity=48.1171548117155, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27963; Mature: 27963

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDILTLFPEFFETPLRIGLVGKALQQGIAEVHCTNPRDFATDKHRRVDDEPYGGGVGM
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCHHCCCHHCCCCCCCCCCCEEE
VLKPEPFFAAVASLPRLDPCEIILLTPQGQPLNQALLQELAQKAQLILLCGQYEGFDERI
EECCCHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHH
RQYLATREVSLGDFVLTGGEIPALALINGVVRLLPGTVGKVDSLKSESFQAGLLDYPQYT
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
RPAEFQGHKVPPVLLSGDHQAIARWRLQQQLVRTWQRRPDLLAKRPLTAEEQQLLAEGLA
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHH
EQHPGVNDVEK
HHCCCCCCCCC
>Mature Secondary Structure
MRFDILTLFPEFFETPLRIGLVGKALQQGIAEVHCTNPRDFATDKHRRVDDEPYGGGVGM
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCHHCCCHHCCCCCCCCCCCEEE
VLKPEPFFAAVASLPRLDPCEIILLTPQGQPLNQALLQELAQKAQLILLCGQYEGFDERI
EECCCHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHH
RQYLATREVSLGDFVLTGGEIPALALINGVVRLLPGTVGKVDSLKSESFQAGLLDYPQYT
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
RPAEFQGHKVPPVLLSGDHQAIARWRLQQQLVRTWQRRPDLLAKRPLTAEEQQLLAEGLA
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHH
EQHPGVNDVEK
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA