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The map label for this gene is proS [H]

Identifier: 86608604

GI number: 86608604

Start: 1162028

End: 1163857

Strand: Reverse

Name: proS [H]

Synonym: CYB_1126

Alternate gene names: 86608604

Gene position: 1163857-1162028 (Counterclockwise)

Preceding gene: 86608605

Following gene: 86608603

Centisome position: 38.2

GC content: 60.6

Gene sequence:

>1830_bases
ATGCAACAAAGCCAGAGACTGAGCCAGATGCTGTTTGTAACGCTGCGGGAGGATCCCGTCGAGGCCGAGTTGCCCAGCCA
CAAATTGCTGCTGCGGGGCTGCTTTATCCGGCGGCTCTCCCCAGGGATCTACACCTATCTGCCGCTCCTCTGGCGGGTGC
TGCAAAAGATCTCGGAGATCGTTCGCCAGGAGATGAACGCCATCGGTGGGCAAGAGTGTCTGCTGCCCCAGTTGCAGCCG
GCGGAGATCTGGCGGGAATCGGGGCGATGGGATGTGTACACCCAAGCCGAGGGGATCATGTTTGCCCTCAAGGACAGGCA
GGGGCGGGAACAGAGCCTCGGCCCCACCCACGAAGAGGTGATCACCCTACTGGCCCGCGACCTGATCCGTTCTTACCGGC
AATTGCCCCAAACCCTCTATCAGATCCAGACCAAGTTTCGGGATGAGATTCGGCCCCGCTTTGGCCTAATGCGAGGGCGG
GAATTCATCATGAAAGACGCCTATTCCTTCCACGCCAACGAGGAAAGCCTGCGCCAGACCTACCAAGATATGTACCGAGC
CTATAGCCGCATCCTCAGGCGCTGTGGGCTGGAGTTTCGAGTGGTGGATGCCGATGCGGGGGCAATCGGGGGTCCATCGG
CGGCCTCGCAGGAGTTTATGGTGCTGGCCTCTGCGGGAGAAGATGAGATCCTCTACACGCCCGATGGCTCCTATGCTGCC
AATGTGGAGAAGGCCGTCTCCATCCCGCCGCCAGCGGCAGAGTCTCCCTACACCGCCTTCCAGGTTCTGGATACGCCGGG
AACCGAAACCATCGAGAAGCTGGCCGCTTTCCTAAAAATCTCCTCTACCCTGATCGTTAAAAACGTCCTCTACCAAGCCC
TTTACGACAACGGCCTGCGGGTGGCAGTGCTGCTCAGCATCCGCGGGGATCAAGAAGTCAATGAGGTCAAACTGGCCAAC
CACCTCAGCCGCCTGGCAGAGCGCTACCAAGCCAGCAAATTGCTCAAGCTCGCCTTGGCCGATGCCGAGATCCAAAACAG
TTGGCAGGGGGATCCCATCCCGGTGGGCTACATTGCCCCCGACTTGCCGGATAGCTGTCTGGGCCGGCAAAAAAACCTTG
CCCCCCAGATCCTGCGCTTGGCCGACCAGACGGCAGCCCTGCTGCAGAACTTCGTAACCGGCGGCAACCAGGTGGGGCAG
CACGTGGTGGGGGCCAACTGGGGCGAGCAATATCCCCTGCCGGAAGTGGTGGATGTGCGCAAGGCACAGGCGGGGGATCG
GTGTTTGCTCAACCCCGACCAGATCCTGGAAACGGCCCGTGGCATCGAGATCGGCCATATCTTTCAGCTAGGCCTCAAGT
TCTCGCTGCCAATGCGGGCCACCTTTACCGACGAACAGGGATCCGAGCAGCCCCTCTGGATGGGCTGTTATGGCATTGGG
GTATCCCGCTTGGCCCAGGCGGCGGTGGAACAGTCCCACGATGCCAACGGGATCATCTGGCCCGTGGCCATTGCCCCCTA
TCATGTGGTGGTGGTAGTGCCCAACATCTCCGACCCCGAGCAGATGAGCGTGGCGGAAAAACTGGTGCAAGATCTCGCTA
CTGCCGGCATCGAGACCCTCTGGGATGACCGGGACGAGCGGGCCGGGGTGAAATTCAAAGATGCGGATCTGATCGGGATC
CCTTACCGCATCACCACAGGGCGCAGCCTCAAACAGGGCAAGGTGGAACTCACCGAGCGGGCCAGCGGCCAAGCCACGGA
AATTCCCATTGAAGAAGTGGTGACTACCCTGAAAGAGCGGATTGAAGCAGCCCTAGCTGTGCCGGACTGA

Upstream 100 bases:

>100_bases
TGCCGGTCAGGCCTGTGTTCTCGGGCAGGGGATCCCAAACAGGGCGGGAAAGTTTGAGCTGATCGTGCGAGCGGGAGGAG
TCCTCAGAGGGAGGGAAGGG

Downstream 100 bases:

>100_bases
GCGTGGCTTCTCCTGAACCCGATGCTGAACATCTCCATTCTTAAAGAAGACGAATCCTATACTTTTCGCTCTTATTTTGA
GATGCCCTACGAGCCAGATG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MQQSQRLSQMLFVTLREDPVEAELPSHKLLLRGCFIRRLSPGIYTYLPLLWRVLQKISEIVRQEMNAIGGQECLLPQLQP
AEIWRESGRWDVYTQAEGIMFALKDRQGREQSLGPTHEEVITLLARDLIRSYRQLPQTLYQIQTKFRDEIRPRFGLMRGR
EFIMKDAYSFHANEESLRQTYQDMYRAYSRILRRCGLEFRVVDADAGAIGGPSAASQEFMVLASAGEDEILYTPDGSYAA
NVEKAVSIPPPAAESPYTAFQVLDTPGTETIEKLAAFLKISSTLIVKNVLYQALYDNGLRVAVLLSIRGDQEVNEVKLAN
HLSRLAERYQASKLLKLALADAEIQNSWQGDPIPVGYIAPDLPDSCLGRQKNLAPQILRLADQTAALLQNFVTGGNQVGQ
HVVGANWGEQYPLPEVVDVRKAQAGDRCLLNPDQILETARGIEIGHIFQLGLKFSLPMRATFTDEQGSEQPLWMGCYGIG
VSRLAQAAVEQSHDANGIIWPVAIAPYHVVVVVPNISDPEQMSVAEKLVQDLATAGIETLWDDRDERAGVKFKDADLIGI
PYRITTGRSLKQGKVELTERASGQATEIPIEEVVTTLKERIEAALAVPD

Sequences:

>Translated_609_residues
MQQSQRLSQMLFVTLREDPVEAELPSHKLLLRGCFIRRLSPGIYTYLPLLWRVLQKISEIVRQEMNAIGGQECLLPQLQP
AEIWRESGRWDVYTQAEGIMFALKDRQGREQSLGPTHEEVITLLARDLIRSYRQLPQTLYQIQTKFRDEIRPRFGLMRGR
EFIMKDAYSFHANEESLRQTYQDMYRAYSRILRRCGLEFRVVDADAGAIGGPSAASQEFMVLASAGEDEILYTPDGSYAA
NVEKAVSIPPPAAESPYTAFQVLDTPGTETIEKLAAFLKISSTLIVKNVLYQALYDNGLRVAVLLSIRGDQEVNEVKLAN
HLSRLAERYQASKLLKLALADAEIQNSWQGDPIPVGYIAPDLPDSCLGRQKNLAPQILRLADQTAALLQNFVTGGNQVGQ
HVVGANWGEQYPLPEVVDVRKAQAGDRCLLNPDQILETARGIEIGHIFQLGLKFSLPMRATFTDEQGSEQPLWMGCYGIG
VSRLAQAAVEQSHDANGIIWPVAIAPYHVVVVVPNISDPEQMSVAEKLVQDLATAGIETLWDDRDERAGVKFKDADLIGI
PYRITTGRSLKQGKVELTERASGQATEIPIEEVVTTLKERIEAALAVPD
>Mature_609_residues
MQQSQRLSQMLFVTLREDPVEAELPSHKLLLRGCFIRRLSPGIYTYLPLLWRVLQKISEIVRQEMNAIGGQECLLPQLQP
AEIWRESGRWDVYTQAEGIMFALKDRQGREQSLGPTHEEVITLLARDLIRSYRQLPQTLYQIQTKFRDEIRPRFGLMRGR
EFIMKDAYSFHANEESLRQTYQDMYRAYSRILRRCGLEFRVVDADAGAIGGPSAASQEFMVLASAGEDEILYTPDGSYAA
NVEKAVSIPPPAAESPYTAFQVLDTPGTETIEKLAAFLKISSTLIVKNVLYQALYDNGLRVAVLLSIRGDQEVNEVKLAN
HLSRLAERYQASKLLKLALADAEIQNSWQGDPIPVGYIAPDLPDSCLGRQKNLAPQILRLADQTAALLQNFVTGGNQVGQ
HVVGANWGEQYPLPEVVDVRKAQAGDRCLLNPDQILETARGIEIGHIFQLGLKFSLPMRATFTDEQGSEQPLWMGCYGIG
VSRLAQAAVEQSHDANGIIWPVAIAPYHVVVVVPNISDPEQMSVAEKLVQDLATAGIETLWDDRDERAGVKFKDADLIGI
PYRITTGRSLKQGKVELTERASGQATEIPIEEVVTTLKERIEAALAVPD

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=259, Percent_Identity=42.0849420849421, Blast_Score=201, Evalue=2e-51,
Organism=Escherichia coli, GI1786392, Length=602, Percent_Identity=43.5215946843854, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI115532348, Length=214, Percent_Identity=35.5140186915888, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320931, Length=237, Percent_Identity=35.8649789029536, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24656200, Length=219, Percent_Identity=38.3561643835616, Blast_Score=158, Evalue=1e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 67906; Mature: 67906

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQSQRLSQMLFVTLREDPVEAELPSHKLLLRGCFIRRLSPGIYTYLPLLWRVLQKISEI
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VRQEMNAIGGQECLLPQLQPAEIWRESGRWDVYTQAEGIMFALKDRQGREQSLGPTHEEV
HHHHHHHCCCCHHCCCCCCHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHH
ITLLARDLIRSYRQLPQTLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFHANEESLRQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YQDMYRAYSRILRRCGLEFRVVDADAGAIGGPSAASQEFMVLASAGEDEILYTPDGSYAA
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCC
NVEKAVSIPPPAAESPYTAFQVLDTPGTETIEKLAAFLKISSTLIVKNVLYQALYDNGLR
CCHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VAVLLSIRGDQEVNEVKLANHLSRLAERYQASKLLKLALADAEIQNSWQGDPIPVGYIAP
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
DLPDSCLGRQKNLAPQILRLADQTAALLQNFVTGGNQVGQHVVGANWGEQYPLPEVVDVR
CCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHH
KAQAGDRCLLNPDQILETARGIEIGHIFQLGLKFSLPMRATFTDEQGSEQPLWMGCYGIG
HHCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCEECCCCEEEECCCCCCCCCEEEEHHHHH
VSRLAQAAVEQSHDANGIIWPVAIAPYHVVVVVPNISDPEQMSVAEKLVQDLATAGIETL
HHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
WDDRDERAGVKFKDADLIGIPYRITTGRSLKQGKVELTERASGQATEIPIEEVVTTLKER
HCCCCHHCCCEEECCCEEECCEEECCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
IEAALAVPD
HHHHHCCCC
>Mature Secondary Structure
MQQSQRLSQMLFVTLREDPVEAELPSHKLLLRGCFIRRLSPGIYTYLPLLWRVLQKISEI
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VRQEMNAIGGQECLLPQLQPAEIWRESGRWDVYTQAEGIMFALKDRQGREQSLGPTHEEV
HHHHHHHCCCCHHCCCCCCHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHH
ITLLARDLIRSYRQLPQTLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFHANEESLRQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YQDMYRAYSRILRRCGLEFRVVDADAGAIGGPSAASQEFMVLASAGEDEILYTPDGSYAA
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCC
NVEKAVSIPPPAAESPYTAFQVLDTPGTETIEKLAAFLKISSTLIVKNVLYQALYDNGLR
CCHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VAVLLSIRGDQEVNEVKLANHLSRLAERYQASKLLKLALADAEIQNSWQGDPIPVGYIAP
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
DLPDSCLGRQKNLAPQILRLADQTAALLQNFVTGGNQVGQHVVGANWGEQYPLPEVVDVR
CCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHH
KAQAGDRCLLNPDQILETARGIEIGHIFQLGLKFSLPMRATFTDEQGSEQPLWMGCYGIG
HHCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCEECCCCEEEECCCCCCCCCEEEEHHHHH
VSRLAQAAVEQSHDANGIIWPVAIAPYHVVVVVPNISDPEQMSVAEKLVQDLATAGIETL
HHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
WDDRDERAGVKFKDADLIGIPYRITTGRSLKQGKVELTERASGQATEIPIEEVVTTLKER
HCCCCHHCCCEEECCCEEECCEEECCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
IEAALAVPD
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA