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The map label for this gene is recJ-2 [H]

Identifier: 86608584

GI number: 86608584

Start: 1140273

End: 1142549

Strand: Direct

Name: recJ-2 [H]

Synonym: CYB_1106

Alternate gene names: 86608584

Gene position: 1140273-1142549 (Clockwise)

Preceding gene: 86608579

Following gene: 86608585

Centisome position: 37.43

GC content: 63.29

Gene sequence:

>2277_bases
ATGGAATACCGCTGGGAGCTGCTGCCCACTCCCCCCCAGTTCAATCCGCAACTGAGCCGGGAATATGGCAAGTATGCGGC
CAAGGTGATGGCTCTGCGGGGTCTGCACACCTGGGAGCAGGCGCAGGCCTTCGTGGATCCCGAACACTATTCCCCCACAC
CACCAGCATCCCTGCCGGATCTGGAGCGGGCGGTGGCGCGGCTGCTGCAGGCACGGGAAAGGGAAGAGACCCTGTGCATT
TGGGGGGACTTTGACTGCGATGGTGTCACCAGTACCAGTCTCCTCCTCTCGGCGTTACAACCCTTGGGCTTCCGAGTCCA
GTTCTCCATACCGCTGCGCAGCACCGAGGGGCACGGGCTCAATCCTCAACGGCTACAGCAGGTGCTGGCCCAGGGCTGCC
AAGTGATCCTGACGGTGGATTGCGGGGTGGGCAATGCTGCCGAAATTGCCATGGCCCAAGCCCAGGGGGTGGACGTGATC
GTGACGGATCACCACATGCTGCCGGATCCCTTGCCCGCTGCCTATGCGGTGGTCAACCCCTTGCGATTGCCGGCTGAACA
TCCGCTGCGCTTCTTGCCGGGGGTGGGAGTGGCCTACAAGTTGGCGGAGGCGCTCTACCAAGCCCTAGGGATCCCTGGTG
TCGAGCAGCACCTGGATCTGGTGGCGGTGGGAATTGTGGCCGATGTGGCGGTGTTGCAGCGGGAGTGCCGCTACTTGGTG
CAGCGGGGCCTGCCGGTGCTGGCCAGTACGCAGCCCAGTACGCAGCGTCCGGGGCTGCAGGCTCTCCTGGCTCGGGTTGA
CAACGGCCAATCCCAGGGGGTGCCGACAGCCCAGGATATTGCTTTTCGCATCGCCCCCAAGTTGAACGTCATTGGCCGCT
TGGAAGACGCCAGCTTAGCAGTGGAGCTGCTGACCACCTCCGATCCAGAGCGGGCCCAGGCGCTGGTGCAACGGTTTGTT
GCCCTTGACGAAGAGCGCCGGCAGCTCACCCAGCAAGTGATCCAAGAGGCCAGCCAACAGGTAGAGAGCTTGGATCTGAC
CCGGGAAGGAGCCATTGTTCTGGCGGAAGCCGGCTGGAACCCAGGGGTTCTAGGGATCGCGGCGGCGCACTTGGTGGAGC
AGTATAACTGCCCAACGGTGTTGATCGCCAAAGACGAAGCGGCGGGACGGGGCTATGGGTCGGGCCGTTCCCTGCCAGGG
GTGAACCTGGTGGAGGCCATCGAGGCCGTGCGCCCCCTCTTGCGGCGGGGAGGCGGACACCCCATGGCCGCCAGGATCCA
AGTGGATCTGGCTTGCCTGGCCGCTGTGCAACTGGCGTTGCAGCGGGAGCTGGCCGCGCGGGTTAGCCCGGATCAGCAGG
CTCGCGCCTTGGCGGTGGAGATTATCCTCGACTGGGAGCTACTGGTCTCTCAGGATCGGGATGGGGTGGCAGAGCTGGAT
GAGGTGTATCGGCAATTGCAGCTTCTGCAGCCCTTTGGGCACGGCAACCCCAACCCCGTCGTTGCCTTGATGAACTTCCG
TCCCAATAGCTCCAACCTGGGAGTCTCCCGCAATGGCCGCCACCTCAAGTTTGAACTCCAAGGCTCGCCGGGATCCCGTA
CCCTGTGGTTTTGGCAGGCCGGAGAAGAGCTGGAGCGTTGGCAAAAATGCCAAGCTTTGGACATCGCCCTCCAGTTGGAA
GCGGATGACCGGGGCAAACAGCCTTGGCAGGGCAAGGTGCTGGCAGTGCGCCCTTCGGGAGAGTGGCAGGCACCCACCCT
TTCTGCTCCCCCCATAGAGATCCAAGATTTTCGGCAGAAACCCCTGCCGGATTCGCTGAAAGGGGCCATCTGTTACGACG
GGCAGGAGGCCGAGAACCTTGTTCCACCACCAAAAGGGGCAAAGCTGGTGCTGGCCCGCTGGCCCTGGCTACCGGCGGAC
TTGGCCCAACTGCTGCAACAGGTACAGCCCCGAACCCTGATCCTGGCCGCTTCTGCCTCCCCCTGGGATCAGATCCCCGC
CCGAGTCAACTGGCTGATGCAACAGTGGCAGGCAGGCCGACGGGATCCCGACGAGCTGGCTGCGGCAACCGGCTTTCCCC
GGCAGTGGCTGGCGGAACTGGACAGTCCGGAGCAGATCCCCACGCTGCTGATGGGCCGCCTTCAGGAATCCCAAGCCTTT
CACCGCTGGCTGGACGAGGCTAGCCTCACCCAGATTGCCCAACTGTGTCAGCGGCTAATGCAATCGGATCCCCTCTCAAG
AGAGACAAGAGAGAAAAGAGAAAAGAACGGAGAGTAG

Upstream 100 bases:

>100_bases
CAAGGTAGCCCATCATCCCCAGCCCTTTCCTCTGGGATCCATTTCCCCCCTGGGCCGTTTTTGATAGGCTCCTAGGAACC
TTTATCCCTGTCGAGCTTGC

Downstream 100 bases:

>100_bases
TGAGAAGGGAGTATCCTCACTCCTCTCTCCTCACTTCTCACTCCTCATTGTTGCTCCCCACCGCTGACGCGATACTCTGG
ATCAAATGGCTCAACCTCAT

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MEYRWELLPTPPQFNPQLSREYGKYAAKVMALRGLHTWEQAQAFVDPEHYSPTPPASLPDLERAVARLLQAREREETLCI
WGDFDCDGVTSTSLLLSALQPLGFRVQFSIPLRSTEGHGLNPQRLQQVLAQGCQVILTVDCGVGNAAEIAMAQAQGVDVI
VTDHHMLPDPLPAAYAVVNPLRLPAEHPLRFLPGVGVAYKLAEALYQALGIPGVEQHLDLVAVGIVADVAVLQRECRYLV
QRGLPVLASTQPSTQRPGLQALLARVDNGQSQGVPTAQDIAFRIAPKLNVIGRLEDASLAVELLTTSDPERAQALVQRFV
ALDEERRQLTQQVIQEASQQVESLDLTREGAIVLAEAGWNPGVLGIAAAHLVEQYNCPTVLIAKDEAAGRGYGSGRSLPG
VNLVEAIEAVRPLLRRGGGHPMAARIQVDLACLAAVQLALQRELAARVSPDQQARALAVEIILDWELLVSQDRDGVAELD
EVYRQLQLLQPFGHGNPNPVVALMNFRPNSSNLGVSRNGRHLKFELQGSPGSRTLWFWQAGEELERWQKCQALDIALQLE
ADDRGKQPWQGKVLAVRPSGEWQAPTLSAPPIEIQDFRQKPLPDSLKGAICYDGQEAENLVPPPKGAKLVLARWPWLPAD
LAQLLQQVQPRTLILAASASPWDQIPARVNWLMQQWQAGRRDPDELAAATGFPRQWLAELDSPEQIPTLLMGRLQESQAF
HRWLDEASLTQIAQLCQRLMQSDPLSRETREKREKNGE

Sequences:

>Translated_758_residues
MEYRWELLPTPPQFNPQLSREYGKYAAKVMALRGLHTWEQAQAFVDPEHYSPTPPASLPDLERAVARLLQAREREETLCI
WGDFDCDGVTSTSLLLSALQPLGFRVQFSIPLRSTEGHGLNPQRLQQVLAQGCQVILTVDCGVGNAAEIAMAQAQGVDVI
VTDHHMLPDPLPAAYAVVNPLRLPAEHPLRFLPGVGVAYKLAEALYQALGIPGVEQHLDLVAVGIVADVAVLQRECRYLV
QRGLPVLASTQPSTQRPGLQALLARVDNGQSQGVPTAQDIAFRIAPKLNVIGRLEDASLAVELLTTSDPERAQALVQRFV
ALDEERRQLTQQVIQEASQQVESLDLTREGAIVLAEAGWNPGVLGIAAAHLVEQYNCPTVLIAKDEAAGRGYGSGRSLPG
VNLVEAIEAVRPLLRRGGGHPMAARIQVDLACLAAVQLALQRELAARVSPDQQARALAVEIILDWELLVSQDRDGVAELD
EVYRQLQLLQPFGHGNPNPVVALMNFRPNSSNLGVSRNGRHLKFELQGSPGSRTLWFWQAGEELERWQKCQALDIALQLE
ADDRGKQPWQGKVLAVRPSGEWQAPTLSAPPIEIQDFRQKPLPDSLKGAICYDGQEAENLVPPPKGAKLVLARWPWLPAD
LAQLLQQVQPRTLILAASASPWDQIPARVNWLMQQWQAGRRDPDELAAATGFPRQWLAELDSPEQIPTLLMGRLQESQAF
HRWLDEASLTQIAQLCQRLMQSDPLSRETREKREKNGE
>Mature_758_residues
MEYRWELLPTPPQFNPQLSREYGKYAAKVMALRGLHTWEQAQAFVDPEHYSPTPPASLPDLERAVARLLQAREREETLCI
WGDFDCDGVTSTSLLLSALQPLGFRVQFSIPLRSTEGHGLNPQRLQQVLAQGCQVILTVDCGVGNAAEIAMAQAQGVDVI
VTDHHMLPDPLPAAYAVVNPLRLPAEHPLRFLPGVGVAYKLAEALYQALGIPGVEQHLDLVAVGIVADVAVLQRECRYLV
QRGLPVLASTQPSTQRPGLQALLARVDNGQSQGVPTAQDIAFRIAPKLNVIGRLEDASLAVELLTTSDPERAQALVQRFV
ALDEERRQLTQQVIQEASQQVESLDLTREGAIVLAEAGWNPGVLGIAAAHLVEQYNCPTVLIAKDEAAGRGYGSGRSLPG
VNLVEAIEAVRPLLRRGGGHPMAARIQVDLACLAAVQLALQRELAARVSPDQQARALAVEIILDWELLVSQDRDGVAELD
EVYRQLQLLQPFGHGNPNPVVALMNFRPNSSNLGVSRNGRHLKFELQGSPGSRTLWFWQAGEELERWQKCQALDIALQLE
ADDRGKQPWQGKVLAVRPSGEWQAPTLSAPPIEIQDFRQKPLPDSLKGAICYDGQEAENLVPPPKGAKLVLARWPWLPAD
LAQLLQQVQPRTLILAASASPWDQIPARVNWLMQQWQAGRRDPDELAAATGFPRQWLAELDSPEQIPTLLMGRLQESQAF
HRWLDEASLTQIAQLCQRLMQSDPLSRETREKREKNGE

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=445, Percent_Identity=33.4831460674157, Blast_Score=209, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 83596; Mature: 83596

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYRWELLPTPPQFNPQLSREYGKYAAKVMALRGLHTWEQAQAFVDPEHYSPTPPASLPD
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCH
LERAVARLLQAREREETLCIWGDFDCDGVTSTSLLLSALQPLGFRVQFSIPLRSTEGHGL
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
NPQRLQQVLAQGCQVILTVDCGVGNAAEIAMAQAQGVDVIVTDHHMLPDPLPAAYAVVNP
CHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHCC
LRLPAEHPLRFLPGVGVAYKLAEALYQALGIPGVEQHLDLVAVGIVADVAVLQRECRYLV
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRGLPVLASTQPSTQRPGLQALLARVDNGQSQGVPTAQDIAFRIAPKLNVIGRLEDASLA
HCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHEEECCCCCCEECCCCHHEE
VELLTTSDPERAQALVQRFVALDEERRQLTQQVIQEASQQVESLDLTREGAIVLAEAGWN
EEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
PGVLGIAAAHLVEQYNCPTVLIAKDEAAGRGYGSGRSLPGVNLVEAIEAVRPLLRRGGGH
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
PMAARIQVDLACLAAVQLALQRELAARVSPDQQARALAVEIILDWELLVSQDRDGVAELD
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEHEEEEHHHHHCCCCCCHHHHH
EVYRQLQLLQPFGHGNPNPVVALMNFRPNSSNLGVSRNGRHLKFELQGSPGSRTLWFWQA
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECC
GEELERWQKCQALDIALQLEADDRGKQPWQGKVLAVRPSGEWQAPTLSAPPIEIQDFRQK
CHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCC
PLPDSLKGAICYDGQEAENLVPPPKGAKLVLARWPWLPADLAQLLQQVQPRTLILAASAS
CCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCC
PWDQIPARVNWLMQQWQAGRRDPDELAAATGFPRQWLAELDSPEQIPTLLMGRLQESQAF
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
HRWLDEASLTQIAQLCQRLMQSDPLSRETREKREKNGE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MEYRWELLPTPPQFNPQLSREYGKYAAKVMALRGLHTWEQAQAFVDPEHYSPTPPASLPD
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCH
LERAVARLLQAREREETLCIWGDFDCDGVTSTSLLLSALQPLGFRVQFSIPLRSTEGHGL
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
NPQRLQQVLAQGCQVILTVDCGVGNAAEIAMAQAQGVDVIVTDHHMLPDPLPAAYAVVNP
CHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHCC
LRLPAEHPLRFLPGVGVAYKLAEALYQALGIPGVEQHLDLVAVGIVADVAVLQRECRYLV
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRGLPVLASTQPSTQRPGLQALLARVDNGQSQGVPTAQDIAFRIAPKLNVIGRLEDASLA
HCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHEEECCCCCCEECCCCHHEE
VELLTTSDPERAQALVQRFVALDEERRQLTQQVIQEASQQVESLDLTREGAIVLAEAGWN
EEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
PGVLGIAAAHLVEQYNCPTVLIAKDEAAGRGYGSGRSLPGVNLVEAIEAVRPLLRRGGGH
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
PMAARIQVDLACLAAVQLALQRELAARVSPDQQARALAVEIILDWELLVSQDRDGVAELD
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEHEEEEHHHHHCCCCCCHHHHH
EVYRQLQLLQPFGHGNPNPVVALMNFRPNSSNLGVSRNGRHLKFELQGSPGSRTLWFWQA
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECC
GEELERWQKCQALDIALQLEADDRGKQPWQGKVLAVRPSGEWQAPTLSAPPIEIQDFRQK
CHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCC
PLPDSLKGAICYDGQEAENLVPPPKGAKLVLARWPWLPADLAQLLQQVQPRTLILAASAS
CCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCC
PWDQIPARVNWLMQQWQAGRRDPDELAAATGFPRQWLAELDSPEQIPTLLMGRLQESQAF
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
HRWLDEASLTQIAQLCQRLMQSDPLSRETREKREKNGE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]