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The map label for this gene is pth

Identifier: 86608210

GI number: 86608210

Start: 744475

End: 745149

Strand: Reverse

Name: pth

Synonym: CYB_0725

Alternate gene names: 86608210

Gene position: 745149-744475 (Counterclockwise)

Preceding gene: 86608213

Following gene: 86608209

Centisome position: 24.46

GC content: 59.56

Gene sequence:

>675_bases
GTGACCCAGGTCAAGCCCAAGGAGGTAGATGAGATTTCCCTGCAGCTTGTTGTGGGCTTGGGCAATCCCGGCCCTCAATA
CGCCAATACTCGCCACAATTGCGGCTTTATGGTGGTGGATCGGCTGGCGCAGCGCTGGGGGATCCCGTTGGTTTTGGAGA
AGCGATTCCAAGGTAGCTACGGAGAAGGGTTTGCCCTGGGAGGAAAGCGACGCTTGCTCAAGCCGGAAACCTACATGAAC
CGTTCCGGCCAATCGGTGCGGGCGGTGCTGGATTGGTACAAGTTAGATCTCGCCTCTGTGCTGGTGGTGTACGACGATAT
GGATCTGCCGCTGGGTCGTCTGCGCCTGCGGGGATCCGGTTCTGCCGGTGGGCACAACGGCATGAAGTCCATCATTCAGC
ACTTGGGGAGCGAGGCGTTTCCACGGCTGCGTTTGGGGGTGGGCAAACCCAAGGGCAGCCAAGATGTAGTCGGGCATGTG
TTGGGTGGATTCGCCCCTGCAGAACAAGAGGTGCTGGAGCGGGTGTTGGATGTGGCGGTAGAGGCCGTGGAGTGCTGCTG
GCAAGAGGGACTGAGGACAGCCATGAACCGCTTTAATCCTCTGGATCTGTCTGGGCCATCGTCCGATTTGGATGGATCCA
ACCCTGCACCGGGCCATGGCGAGGCTTCCTCCTGA

Upstream 100 bases:

>100_bases
CTGGGTATCCGAACCTCCGGGGCGATCAGCAGCCCGAAAACACGCCAACACCACTCAAAGCAGCGATGCCTTACTTTAGC
TCGAGGAGGCGAGAAACGTT

Downstream 100 bases:

>100_bases
GGAGCAGCTCGTCGTCCACCTGGGTTGTTGGCTCTGAGGGATTTTCCAGGAGCGGGGTGATAAGGGCTTCGTCCTGCTCA
TGGGAACGTCTGTGGAGACA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTQVKPKEVDEISLQLVVGLGNPGPQYANTRHNCGFMVVDRLAQRWGIPLVLEKRFQGSYGEGFALGGKRRLLKPETYMN
RSGQSVRAVLDWYKLDLASVLVVYDDMDLPLGRLRLRGSGSAGGHNGMKSIIQHLGSEAFPRLRLGVGKPKGSQDVVGHV
LGGFAPAEQEVLERVLDVAVEAVECCWQEGLRTAMNRFNPLDLSGPSSDLDGSNPAPGHGEASS

Sequences:

>Translated_224_residues
MTQVKPKEVDEISLQLVVGLGNPGPQYANTRHNCGFMVVDRLAQRWGIPLVLEKRFQGSYGEGFALGGKRRLLKPETYMN
RSGQSVRAVLDWYKLDLASVLVVYDDMDLPLGRLRLRGSGSAGGHNGMKSIIQHLGSEAFPRLRLGVGKPKGSQDVVGHV
LGGFAPAEQEVLERVLDVAVEAVECCWQEGLRTAMNRFNPLDLSGPSSDLDGSNPAPGHGEASS
>Mature_223_residues
TQVKPKEVDEISLQLVVGLGNPGPQYANTRHNCGFMVVDRLAQRWGIPLVLEKRFQGSYGEGFALGGKRRLLKPETYMNR
SGQSVRAVLDWYKLDLASVLVVYDDMDLPLGRLRLRGSGSAGGHNGMKSIIQHLGSEAFPRLRLGVGKPKGSQDVVGHVL
GGFAPAEQEVLERVLDVAVEAVECCWQEGLRTAMNRFNPLDLSGPSSDLDGSNPAPGHGEASS

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=170, Percent_Identity=41.7647058823529, Blast_Score=121, Evalue=6e-28,
Organism=Escherichia coli, GI1787455, Length=190, Percent_Identity=42.1052631578947, Blast_Score=132, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321983, Length=174, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_SYNJB (Q2JNF7)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_476972.1
- ProteinModelPortal:   Q2JNF7
- SMR:   Q2JNF7
- STRING:   Q2JNF7
- GeneID:   3902352
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_0725
- TIGR:   CYB_0725
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- PhylomeDB:   Q2JNF7
- ProtClustDB:   PRK05426
- BioCyc:   SSP321332:CYB_0725-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 24264; Mature: 24133

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVKPKEVDEISLQLVVGLGNPGPQYANTRHNCGFMVVDRLAQRWGIPLVLEKRFQGSY
CCCCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCC
GEGFALGGKRRLLKPETYMNRSGQSVRAVLDWYKLDLASVLVVYDDMDLPLGRLRLRGSG
CCCEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHEEECCCC
SAGGHNGMKSIIQHLGSEAFPRLRLGVGKPKGSQDVVGHVLGGFAPAEQEVLERVLDVAV
CCCCHHHHHHHHHHHCHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
EAVECCWQEGLRTAMNRFNPLDLSGPSSDLDGSNPAPGHGEASS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQVKPKEVDEISLQLVVGLGNPGPQYANTRHNCGFMVVDRLAQRWGIPLVLEKRFQGSY
CCCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCC
GEGFALGGKRRLLKPETYMNRSGQSVRAVLDWYKLDLASVLVVYDDMDLPLGRLRLRGSG
CCCEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHEEECCCC
SAGGHNGMKSIIQHLGSEAFPRLRLGVGKPKGSQDVVGHVLGGFAPAEQEVLERVLDVAV
CCCCHHHHHHHHHHHCHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
EAVECCWQEGLRTAMNRFNPLDLSGPSSDLDGSNPAPGHGEASS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA