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The map label for this gene is trpE [H]

Identifier: 86608027

GI number: 86608027

Start: 549636

End: 551156

Strand: Reverse

Name: trpE [H]

Synonym: CYB_0535

Alternate gene names: 86608027

Gene position: 551156-549636 (Counterclockwise)

Preceding gene: 86608030

Following gene: 86608026

Centisome position: 18.09

GC content: 61.21

Gene sequence:

>1521_bases
ATGATTTACCCTCCCTTGAAGGAGTTTCTTGCCCTCACCAAGCAGGGCAACTTTATCCCGGTGTACCAGGAATGGGTGGC
GGATTTGGAAACGCCGGTTTCTGCCTGGTATCGGGTCTGTGCTGGCCAGCCCTACAACTTTCTCCTGGAGTCGGTGGAGG
GGGGGGAACAGGTGGCCCGCTACAGCTTCTTGGGCTGCGATCCCCTGTGGGTTTTGGAAATACGCGGGGATCAGGCGCTA
CAGCGATACCGCGATGGCCGACAGGAGCACCACCAAGGGGATCCCTTTGCCATCTTGGCCCGCTGCTTGCAGCCCTATGA
GCCGGTGCATTTGCCGGAATTGCCCAGCAGCATCGGCGGCCTATTTGGCTACTGGGGCTACGAGCTGATTCGCTGGATTG
AGCCCAAGGTGCCCGTCCACCCCCTGCAGCCGGGGGATCCCCCTGATGCCGTTTTGATGCAGGTGGACAGCATCCTGCTC
TTTGACCAGGTAAAGCGCAAGATTTGGGTGGTGGCCTTCGCCGATACCCGTCACCACGCTCCTGAGGAAGCCTATGCCCA
AGCCTGCCGGCGGGTGGAAAAGCTGATGCAGCAGCTCAGCGCTCCTCTTTCCCTGGCCCATCTGCACCTCAACTGGAAGC
CCCACCTGTCGGAAAGGACTCCGTCCCGCTGGCGCGAACAAATCCCCCCCAGCACCCTGAGCCGCCCGGCCTACTGCCAA
GCGGTGGAACGGGCTAAGGACTACATCCGCGCCGGAGATATCTTCCAGGTGGTGCTCTCGCAGCGGTTTACCGTCTCCCT
TGCCGGGGATCCCTTTCGCCTCTACCGCTCGTTGCGGCTGATTAACCCTTCCCCTTACATGGCGTTTTTGCAGTTTGGGG
ATCTCTGCCTCATCGGCTCCAGCCCAGAGGTGATGGTGAAACTCAGCCGCGTGGGGGAAGACCGCATCGCCACTGTGCGC
CCCATTGCCGGCACCCGCCCCCGCGGCACCACCCCTCTGGAAGACCGGCAGTTAGAGCAAGAGCTGTTGGCAGATCCCAA
AGAACGGGCCGAACATGTGATGCTGGTGGATCTGGCCCGCAATGACCTGGGACGGGTCTGCCAACTGGGATCTGTGCAGG
TGGACGACCTGATGCAGATCGAGCGCTACAGCCATGTGATGCACATCGTCAGCAACGTGGTGGGGCGGCTGGATCCCCAG
TACAGCGCCTGGGATCTGCTGCGGGCCACCTTCCCTGCCGGCACTGTTACCGGCGCCCCCAAAATCCGCGCCATGCAGAT
TATTCATGAGCTGGAAGGATGCCGCCGCGGGCCCTATGCCGGCGCCTATGGCTACTACGACTTTTCTGGCCAACTGAACA
CCGCCATCACCATCCGCACTCTGCTGGTTCATCAGGGCCAGGTGAGCTTGCAGGCCGGGGCAGGTATTGTAGCCGACTCG
GATCCGGAGCGGGAATATCAAGAATGTCTGAACAAAGCGCGGGGCATGTTAATGGCTGTGGCCAGTTTGCAGGAGACCTA
G

Upstream 100 bases:

>100_bases
GTTTCCGGCGCTGTTGCAACCGGGCAATCCAGAGGGATCCACACGCCCTTGGGATCCCGTGTCTGGGATAGAGTCAGGAA
AAGCACAAAAGTTGCAGGCC

Downstream 100 bases:

>100_bases
CCGATGTTGGCCCAGTTGCGCTTCAGCCCTGCGGTTCGCGTCAGCGGGACGGAGTCCTTACAGGAGCTCCAGCAGCCGGA
TCCAGAGACCGTAGAGCAGG

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MIYPPLKEFLALTKQGNFIPVYQEWVADLETPVSAWYRVCAGQPYNFLLESVEGGEQVARYSFLGCDPLWVLEIRGDQAL
QRYRDGRQEHHQGDPFAILARCLQPYEPVHLPELPSSIGGLFGYWGYELIRWIEPKVPVHPLQPGDPPDAVLMQVDSILL
FDQVKRKIWVVAFADTRHHAPEEAYAQACRRVEKLMQQLSAPLSLAHLHLNWKPHLSERTPSRWREQIPPSTLSRPAYCQ
AVERAKDYIRAGDIFQVVLSQRFTVSLAGDPFRLYRSLRLINPSPYMAFLQFGDLCLIGSSPEVMVKLSRVGEDRIATVR
PIAGTRPRGTTPLEDRQLEQELLADPKERAEHVMLVDLARNDLGRVCQLGSVQVDDLMQIERYSHVMHIVSNVVGRLDPQ
YSAWDLLRATFPAGTVTGAPKIRAMQIIHELEGCRRGPYAGAYGYYDFSGQLNTAITIRTLLVHQGQVSLQAGAGIVADS
DPEREYQECLNKARGMLMAVASLQET

Sequences:

>Translated_506_residues
MIYPPLKEFLALTKQGNFIPVYQEWVADLETPVSAWYRVCAGQPYNFLLESVEGGEQVARYSFLGCDPLWVLEIRGDQAL
QRYRDGRQEHHQGDPFAILARCLQPYEPVHLPELPSSIGGLFGYWGYELIRWIEPKVPVHPLQPGDPPDAVLMQVDSILL
FDQVKRKIWVVAFADTRHHAPEEAYAQACRRVEKLMQQLSAPLSLAHLHLNWKPHLSERTPSRWREQIPPSTLSRPAYCQ
AVERAKDYIRAGDIFQVVLSQRFTVSLAGDPFRLYRSLRLINPSPYMAFLQFGDLCLIGSSPEVMVKLSRVGEDRIATVR
PIAGTRPRGTTPLEDRQLEQELLADPKERAEHVMLVDLARNDLGRVCQLGSVQVDDLMQIERYSHVMHIVSNVVGRLDPQ
YSAWDLLRATFPAGTVTGAPKIRAMQIIHELEGCRRGPYAGAYGYYDFSGQLNTAITIRTLLVHQGQVSLQAGAGIVADS
DPEREYQECLNKARGMLMAVASLQET
>Mature_506_residues
MIYPPLKEFLALTKQGNFIPVYQEWVADLETPVSAWYRVCAGQPYNFLLESVEGGEQVARYSFLGCDPLWVLEIRGDQAL
QRYRDGRQEHHQGDPFAILARCLQPYEPVHLPELPSSIGGLFGYWGYELIRWIEPKVPVHPLQPGDPPDAVLMQVDSILL
FDQVKRKIWVVAFADTRHHAPEEAYAQACRRVEKLMQQLSAPLSLAHLHLNWKPHLSERTPSRWREQIPPSTLSRPAYCQ
AVERAKDYIRAGDIFQVVLSQRFTVSLAGDPFRLYRSLRLINPSPYMAFLQFGDLCLIGSSPEVMVKLSRVGEDRIATVR
PIAGTRPRGTTPLEDRQLEQELLADPKERAEHVMLVDLARNDLGRVCQLGSVQVDDLMQIERYSHVMHIVSNVVGRLDPQ
YSAWDLLRATFPAGTVTGAPKIRAMQIIHELEGCRRGPYAGAYGYYDFSGQLNTAITIRTLLVHQGQVSLQAGAGIVADS
DPEREYQECLNKARGMLMAVASLQET

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=398, Percent_Identity=39.6984924623116, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788114, Length=456, Percent_Identity=33.7719298245614, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI87082077, Length=275, Percent_Identity=27.6363636363636, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786809, Length=276, Percent_Identity=29.7101449275362, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=503, Percent_Identity=36.182902584493, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6324361, Length=410, Percent_Identity=26.5853658536585, Blast_Score=143, Evalue=8e-35,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57192; Mature: 57192

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPPLKEFLALTKQGNFIPVYQEWVADLETPVSAWYRVCAGQPYNFLLESVEGGEQVAR
CCCCCHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHH
YSFLGCDPLWVLEIRGDQALQRYRDGRQEHHQGDPFAILARCLQPYEPVHLPELPSSIGG
HHEECCCCEEEEEECCHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHH
LFGYWGYELIRWIEPKVPVHPLQPGDPPDAVLMQVDSILLFDQVKRKIWVVAFADTRHHA
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
PEEAYAQACRRVEKLMQQLSAPLSLAHLHLNWKPHLSERTPSRWREQIPPSTLSRPAYCQ
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCHHCCCCHHHH
AVERAKDYIRAGDIFQVVLSQRFTVSLAGDPFRLYRSLRLINPSPYMAFLQFGDLCLIGS
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHCCEEEECC
SPEVMVKLSRVGEDRIATVRPIAGTRPRGTTPLEDRQLEQELLADPKERAEHVMLVDLAR
CCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCEEEEEHHHH
NDLGRVCQLGSVQVDDLMQIERYSHVMHIVSNVVGRLDPQYSAWDLLRATFPAGTVTGAP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
KIRAMQIIHELEGCRRGPYAGAYGYYDFSGQLNTAITIRTLLVHQGQVSLQAGAGIVADS
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHEEHHHHHHHCCCEEEECCCCEECCC
DPEREYQECLNKARGMLMAVASLQET
CCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYPPLKEFLALTKQGNFIPVYQEWVADLETPVSAWYRVCAGQPYNFLLESVEGGEQVAR
CCCCCHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHH
YSFLGCDPLWVLEIRGDQALQRYRDGRQEHHQGDPFAILARCLQPYEPVHLPELPSSIGG
HHEECCCCEEEEEECCHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHH
LFGYWGYELIRWIEPKVPVHPLQPGDPPDAVLMQVDSILLFDQVKRKIWVVAFADTRHHA
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
PEEAYAQACRRVEKLMQQLSAPLSLAHLHLNWKPHLSERTPSRWREQIPPSTLSRPAYCQ
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCHHCCCCHHHH
AVERAKDYIRAGDIFQVVLSQRFTVSLAGDPFRLYRSLRLINPSPYMAFLQFGDLCLIGS
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHCCEEEECC
SPEVMVKLSRVGEDRIATVRPIAGTRPRGTTPLEDRQLEQELLADPKERAEHVMLVDLAR
CCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCEEEEEHHHH
NDLGRVCQLGSVQVDDLMQIERYSHVMHIVSNVVGRLDPQYSAWDLLRATFPAGTVTGAP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
KIRAMQIIHELEGCRRGPYAGAYGYYDFSGQLNTAITIRTLLVHQGQVSLQAGAGIVADS
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHEEHHHHHHHCCCEEEECCCCEECCC
DPEREYQECLNKARGMLMAVASLQET
CCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 3141423 [H]