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The map label for this gene is proB [H]

Identifier: 86608012

GI number: 86608012

Start: 534374

End: 535513

Strand: Direct

Name: proB [H]

Synonym: CYB_0519

Alternate gene names: 86608012

Gene position: 534374-535513 (Clockwise)

Preceding gene: 86608009

Following gene: 86608014

Centisome position: 17.54

GC content: 62.37

Gene sequence:

>1140_bases
ATGGCAACAACAGACGTGACGGCGATGGCAACAACAGACGTGACGGTGGTGGTGAAGATCGGCACCTCCAGCCTGACAGA
TACTCAGACCGGCTTGTTGCGTCTTTCGGTGTTGGGGCCGCTGGTGGAGGTGCTGACCCACCTGCGCAGGCAGGGGTATG
CGGTGATCTTGGTCTCCTCTGGGGCGGTGGGGGTGGGCTGTGCTCGCCTTGGCTGGCGGCAACGACCGACGGCTATTGCC
GAGAAGCAGGCGGTGGCTGCGGTGGGACAGGGGCGGTTGATCCGCCTTTACGATGACCTCTTCTCGGCTCTGAACCAGCC
AATTGCCCAAGTGCTGCTCACCCGCGGCGATCTGGTGGAGCGCAGCCGCTACGTGAACGCCAATCGCACTTTTGCCCAAC
TGCTGCAGATGGGGGTTATTCCCATCGTCAATGAAAACGACACGGTGGCAGTGGAGGAGCTGAAATTCGGCGATAACGAC
TCGCTTTCGGCGTTAGTGGCCAGCATGGTGCAGGCCAAGTGGCTTATCCTGCTCACCGATGTGGACAAGCTCTATTCGGC
TGACCCCAACCGAGATCCCTCTGCCCAGCCCATTGAGCGGGTGTTGCCCGGGATCCCGCTACAGGTGAAGGCCGAAGCCC
AGGGGAAAAGCGGCTGGGGCACCGGCGGCATGGCCACCAAACTGACCGCTGCTCAAATTGCTACAGCTGCAGGGGTAACG
GTGGTGATCACCAATGGCAAACGGCCTGAGCAGATCCCGGCAATTCTGGCCGGCGAGGCCATCGGCACCCGCTTTGATCC
GGCTCCTCAGCCCGCCAGCGCCCGCAAGCGATGGATTGCCTACGGCCTCATCCCCGAGGGATCTTTGACTTTGGATGAGG
GGGCGGTACGAGCGGTGTGCGAGCAAGGGCGCTCGCTGTTGCCGGCAGGGATCACAGCCATTTCTGGGGAGTTTGAGGCG
GGGGCTGCCGTGCGCCTGTGCGATCCCAGCGGCCAGGAGGTGGCGCGGGGCCTGGTGAACTACAGCGCCGAGGAGCTGCG
GCAGATTAAGGGCAAAAAAACCGCCGAGATTCCCCGCATCCTGGGCTACGAAGGGGTTGATACTGCCGTCCACCGGGATA
ACTTGGCCTTGCTCAATTGA

Upstream 100 bases:

>100_bases
CGGGGCTTGCCGCGCCTTTTGGTCAATACAGCTGCGTCTTGACTCCGCTATGGTGGCGCTATGGTGAATAGAGGGGTGGG
AGGATGGGTTGAGAGCGGCG

Downstream 100 bases:

>100_bases
GGCCATGGGTGAGGCCATGGGGGCGAGTCCAGGCGCGGTGCTCTGCACCACTAGCACGAATGGCTACAACGGCGGGATCC
CAACGCCTATTCTTCCAACT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MATTDVTAMATTDVTVVVKIGTSSLTDTQTGLLRLSVLGPLVEVLTHLRRQGYAVILVSSGAVGVGCARLGWRQRPTAIA
EKQAVAAVGQGRLIRLYDDLFSALNQPIAQVLLTRGDLVERSRYVNANRTFAQLLQMGVIPIVNENDTVAVEELKFGDND
SLSALVASMVQAKWLILLTDVDKLYSADPNRDPSAQPIERVLPGIPLQVKAEAQGKSGWGTGGMATKLTAAQIATAAGVT
VVITNGKRPEQIPAILAGEAIGTRFDPAPQPASARKRWIAYGLIPEGSLTLDEGAVRAVCEQGRSLLPAGITAISGEFEA
GAAVRLCDPSGQEVARGLVNYSAEELRQIKGKKTAEIPRILGYEGVDTAVHRDNLALLN

Sequences:

>Translated_379_residues
MATTDVTAMATTDVTVVVKIGTSSLTDTQTGLLRLSVLGPLVEVLTHLRRQGYAVILVSSGAVGVGCARLGWRQRPTAIA
EKQAVAAVGQGRLIRLYDDLFSALNQPIAQVLLTRGDLVERSRYVNANRTFAQLLQMGVIPIVNENDTVAVEELKFGDND
SLSALVASMVQAKWLILLTDVDKLYSADPNRDPSAQPIERVLPGIPLQVKAEAQGKSGWGTGGMATKLTAAQIATAAGVT
VVITNGKRPEQIPAILAGEAIGTRFDPAPQPASARKRWIAYGLIPEGSLTLDEGAVRAVCEQGRSLLPAGITAISGEFEA
GAAVRLCDPSGQEVARGLVNYSAEELRQIKGKKTAEIPRILGYEGVDTAVHRDNLALLN
>Mature_378_residues
ATTDVTAMATTDVTVVVKIGTSSLTDTQTGLLRLSVLGPLVEVLTHLRRQGYAVILVSSGAVGVGCARLGWRQRPTAIAE
KQAVAAVGQGRLIRLYDDLFSALNQPIAQVLLTRGDLVERSRYVNANRTFAQLLQMGVIPIVNENDTVAVEELKFGDNDS
LSALVASMVQAKWLILLTDVDKLYSADPNRDPSAQPIERVLPGIPLQVKAEAQGKSGWGTGGMATKLTAAQIATAAGVTV
VITNGKRPEQIPAILAGEAIGTRFDPAPQPASARKRWIAYGLIPEGSLTLDEGAVRAVCEQGRSLLPAGITAISGEFEAG
AAVRLCDPSGQEVARGLVNYSAEELRQIKGKKTAEIPRILGYEGVDTAVHRDNLALLN

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=284, Percent_Identity=33.0985915492958, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI62912457, Length=282, Percent_Identity=32.9787234042553, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786437, Length=362, Percent_Identity=44.1988950276243, Blast_Score=293, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=37.0786516853933, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17569969, Length=270, Percent_Identity=37.037037037037, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320506, Length=424, Percent_Identity=38.2075471698113, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321822, Length=426, Percent_Identity=36.150234741784, Blast_Score=250, Evalue=2e-67,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=33.7931034482759, Blast_Score=134, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40148; Mature: 40016

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTDVTAMATTDVTVVVKIGTSSLTDTQTGLLRLSVLGPLVEVLTHLRRQGYAVILVSS
CCCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEC
GAVGVGCARLGWRQRPTAIAEKQAVAAVGQGRLIRLYDDLFSALNQPIAQVLLTRGDLVE
CCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHH
RSRYVNANRTFAQLLQMGVIPIVNENDTVAVEELKFGDNDSLSALVASMVQAKWLILLTD
HHHHCCCCHHHHHHHHHCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEEC
VDKLYSADPNRDPSAQPIERVLPGIPLQVKAEAQGKSGWGTGGMATKLTAAQIATAAGVT
HHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCEE
VVITNGKRPEQIPAILAGEAIGTRFDPAPQPASARKRWIAYGLIPEGSLTLDEGAVRAVC
EEEECCCCCHHCCHHHCCCHHCCCCCCCCCCHHHHHCEEEEEECCCCCEEECHHHHHHHH
EQGRSLLPAGITAISGEFEAGAAVRLCDPSGQEVARGLVNYSAEELRQIKGKKTAEIPRI
HCCCCCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCHH
LGYEGVDTAVHRDNLALLN
HCCCCCCHHHCCCCEEECC
>Mature Secondary Structure 
ATTDVTAMATTDVTVVVKIGTSSLTDTQTGLLRLSVLGPLVEVLTHLRRQGYAVILVSS
CCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEC
GAVGVGCARLGWRQRPTAIAEKQAVAAVGQGRLIRLYDDLFSALNQPIAQVLLTRGDLVE
CCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHH
RSRYVNANRTFAQLLQMGVIPIVNENDTVAVEELKFGDNDSLSALVASMVQAKWLILLTD
HHHHCCCCHHHHHHHHHCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEEC
VDKLYSADPNRDPSAQPIERVLPGIPLQVKAEAQGKSGWGTGGMATKLTAAQIATAAGVT
HHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCEE
VVITNGKRPEQIPAILAGEAIGTRFDPAPQPASARKRWIAYGLIPEGSLTLDEGAVRAVC
EEEECCCCCHHCCHHHCCCHHCCCCCCCCCCHHHHHCEEEEEECCCCCEEECHHHHHHHH
EQGRSLLPAGITAISGEFEAGAAVRLCDPSGQEVARGLVNYSAEELRQIKGKKTAEIPRI
HCCCCCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCHH
LGYEGVDTAVHRDNLALLN
HCCCCCCHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA