Click here to switch to the map view.

The map label for this gene is hisF [H]

Identifier: 86607940

GI number: 86607940

Start: 453281

End: 454042

Strand: Reverse

Name: hisF [H]

Synonym: CYB_0445

Alternate gene names: 86607940

Gene position: 454042-453281 (Counterclockwise)

Preceding gene: 86607941

Following gene: 86607939

Centisome position: 14.9

GC content: 64.57

Gene sequence:

>762_bases
ATGTTGGCCAAACGGATCATTCCCTGTCTGGATGTGGCGGCAGGGCGGGTGGTGAAAGGGATCCAGTTTGTGCAATTGCG
GGATGCCGGGGATCCGGTAGAGCTGGCCCAGGCTTACGATCAGGCAGGAGCCGATGAGCTGGTCTTTTTGGACATCACCG
CCACCCACGAAGAGCGGGAGATCTTGCTGGAGGTGGTGCGGCGCACCGCAGAGCAGGTGTTCATTCCCCTCACGGTGGGA
GGGGGGATCCGCGATCTGGAGGGGATCCGACAACTGCTGCGGGCCGGGGCGGACAAGGTCAGCCTCAACTCGGCAGCGGT
TCGGGATCCTGATCTCGTGGCTCAGGCCAGCCGCTGGTTTGGATCCCAGTGCATTGTGGTGGCCATTGATGCTCGCGCCA
AGCCGCAGGGAGGGGGATGGGAAGTGTACGTGCGCGGGGGGCGGCAGCCGACGGGCCTGGACGCGGTGGAGTGGGCCAAA
AGCGTGGCCGAGCGGGGGGCAGGGGAGATTTTGCTCACCAGCATGGACGCCGACGGTACCCAGGCCGGCTATGACCTGGA
GCTAACCCGGCGGGTGGTGGATGCGGTGTCGGTGCCGGTGATCGCCTCTGGGGGTGCAGGAGCGTGTGCCCACATCCGCC
AGGCTCTGCAGGAAGCGGGAGCAGAAGCAGCGCTGTTGGCTTCTCTGTTGCACTACGGCCAGTTAACGATTGCCCAAATC
AAAGCCGACTTGGCCGCCCACGGCATTCCGGTGCGTTTCTAG

Upstream 100 bases:

>100_bases
AAGTTTCAAGAACCCTTCCTGGTCATCAGCTTCGGCTAAAACGATCCCCAGTTGTTCCCCTCTGTTCGCGTTAGCGGTAT
GGAGTCCTTGGGAGAGAGCG

Downstream 100 bases:

>100_bases
CTTGGGGAGGGATCCGTGCAGGCTCAAGATCTGGCCGCTCTCCTGCCTGAGGTGTGCCGCCTGGCAGAAGCAGCAGGGCA
GGCCATCTTGCCCTTCTATC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLAKRIIPCLDVAAGRVVKGIQFVQLRDAGDPVELAQAYDQAGADELVFLDITATHEEREILLEVVRRTAEQVFIPLTVG
GGIRDLEGIRQLLRAGADKVSLNSAAVRDPDLVAQASRWFGSQCIVVAIDARAKPQGGGWEVYVRGGRQPTGLDAVEWAK
SVAERGAGEILLTSMDADGTQAGYDLELTRRVVDAVSVPVIASGGAGACAHIRQALQEAGAEAALLASLLHYGQLTIAQI
KADLAAHGIPVRF

Sequences:

>Translated_253_residues
MLAKRIIPCLDVAAGRVVKGIQFVQLRDAGDPVELAQAYDQAGADELVFLDITATHEEREILLEVVRRTAEQVFIPLTVG
GGIRDLEGIRQLLRAGADKVSLNSAAVRDPDLVAQASRWFGSQCIVVAIDARAKPQGGGWEVYVRGGRQPTGLDAVEWAK
SVAERGAGEILLTSMDADGTQAGYDLELTRRVVDAVSVPVIASGGAGACAHIRQALQEAGAEAALLASLLHYGQLTIAQI
KADLAAHGIPVRF
>Mature_253_residues
MLAKRIIPCLDVAAGRVVKGIQFVQLRDAGDPVELAQAYDQAGADELVFLDITATHEEREILLEVVRRTAEQVFIPLTVG
GGIRDLEGIRQLLRAGADKVSLNSAAVRDPDLVAQASRWFGSQCIVVAIDARAKPQGGGWEVYVRGGRQPTGLDAVEWAK
SVAERGAGEILLTSMDADGTQAGYDLELTRRVVDAVSVPVIASGGAGACAHIRQALQEAGAEAALLASLLHYGQLTIAQI
KADLAAHGIPVRF

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=45.3125, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=29.7071129707113, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=35.4632587859425, Blast_Score=168, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26780; Mature: 26780

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVAAGRVVKGIQFVQLRDAGDPVELAQAYDQAGADELVFLDITATHEERE
CCHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHH
ILLEVVRRTAEQVFIPLTVGGGIRDLEGIRQLLRAGADKVSLNSAAVRDPDLVAQASRWF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHC
GSQCIVVAIDARAKPQGGGWEVYVRGGRQPTGLDAVEWAKSVAERGAGEILLTSMDADGT
CCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
QAGYDLELTRRVVDAVSVPVIASGGAGACAHIRQALQEAGAEAALLASLLHYGQLTIAQI
CCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHH
KADLAAHGIPVRF
HHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVAAGRVVKGIQFVQLRDAGDPVELAQAYDQAGADELVFLDITATHEERE
CCHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHH
ILLEVVRRTAEQVFIPLTVGGGIRDLEGIRQLLRAGADKVSLNSAAVRDPDLVAQASRWF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHC
GSQCIVVAIDARAKPQGGGWEVYVRGGRQPTGLDAVEWAKSVAERGAGEILLTSMDADGT
CCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
QAGYDLELTRRVVDAVSVPVIASGGAGACAHIRQALQEAGAEAALLASLLHYGQLTIAQI
CCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHH
KADLAAHGIPVRF
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]