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The map label for this gene is aksA [H]

Identifier: 86607921

GI number: 86607921

Start: 434137

End: 435324

Strand: Direct

Name: aksA [H]

Synonym: CYB_0424

Alternate gene names: 86607921

Gene position: 434137-435324 (Clockwise)

Preceding gene: 86607920

Following gene: 86607922

Centisome position: 14.25

GC content: 62.96

Gene sequence:

>1188_bases
ATGAATTCCTCTATCACCATCAACGACACCACCCTACGCGATGGGGAGCAAGCGGCAGGGGTAGCCTTCCAAATCCCCGA
GAAAATCTCCATTGCTGCCCTCTTGGATGCCATTGGCGTGCCCGAGATCGAGGTGGGGATCCCGGCCATGGGCGCCGTGG
AAACGGAAGCCATTCGCGCCATGCTCAGCCTGGGGCTGCGGGCCCACCTGCTGGGGTGGAACCGGGCTGTGCCCAGCGAT
ATCCTCGCCTCTATTCGTTGCGGCCTCAAGCGGATCCACATCTCCGTGCCGGTCTCGGATCGGCAAATCGCTGCCAAGTT
CCAAGGGGATCGCCCTCGGCTGCTGCGACAGTTGCGCCAATGCGTGGAGATGGCCTTGGAGCAAGGGCTGTGGGTCTCGG
TGGGGGCAGAAGATGCCAGCCGCACCCCCCTGGAGCAGGTGATCGAGTTGGCCCAAATGGCCCAAAGCTGGGGCTGTCGG
CGCTTCCGCTACTGCGATACGGTAGGCGTTTTGGATCCCTTTAGCACCTACAAGCAGATTCGCCGTCTGGTGCGGGCCAT
CGATATCCCTGTGGAGATGCATACCCACGACGACCTGGGCATGGCCACTGCCAATGCCTTAGCCGGGATCCGCGCCGGCG
CTCAGTCGGTGAACACCACCGTCAACGGCTTGGGGGAACGGGCCGGCAATGCCCCCTTGGAAGAGGTGGTGATGGCTTTG
CGCTGCACCTTGGGGATCGATGTTGGGGTGGATACCCGCCGTTTGCGGGAGCTGTCTCAACAGGTGGCACGGGCCAGCGG
TGCTCCCCTCCCCCCCTGGAAGGCCATCGTTGGCGACAACGCCTTTGCCCACGAGTCGGGCATTCACGCTGCAGGGGTGC
TACGGGATCCCCAGACCTATGAGCCTTTCCCACCAGAGGCGGTAGGCAGCCAGCGGCGGCTGGTGTTGGGCAAACACTCC
GGCCGGCATTTGCTGCTCCAGATCCTCAAGGAACAGGGGATCCCTGTCGAGCAGATCCCACCTGGCCAACTGGAAAAGCT
CCTGGAACAGGTGCGGCAGCACGTCACCCACTGCAAGCATCCGATCCCACCGGCGGAACTGGCCCGTTATTGCCGCGCAA
CGCTGACGCAATGGGCGCAACACACTCCAGAACCCATCCCAGAGGAGAGCCATCCCGATGTTGTCTAA

Upstream 100 bases:

>100_bases
ATGATTGCCCTGACGCAACCGGGGTCCCGCTAGCGGTGCGGAGCACCATCGACGGTTACCGATTGCCTAGGCGGGCCGTA
GGTCTTGGAGGATGGATCCC

Downstream 100 bases:

>100_bases
CCGCTTGTCCGGAAAGGAAACTTCCAAATCTCAGGCCATTTTGGCAGCCGTGTCTCGACGGGCGCGCCAAGTAGGCCGCA
GCCTAACCCGCCAAGAGCTT

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNSSITINDTTLRDGEQAAGVAFQIPEKISIAALLDAIGVPEIEVGIPAMGAVETEAIRAMLSLGLRAHLLGWNRAVPSD
ILASIRCGLKRIHISVPVSDRQIAAKFQGDRPRLLRQLRQCVEMALEQGLWVSVGAEDASRTPLEQVIELAQMAQSWGCR
RFRYCDTVGVLDPFSTYKQIRRLVRAIDIPVEMHTHDDLGMATANALAGIRAGAQSVNTTVNGLGERAGNAPLEEVVMAL
RCTLGIDVGVDTRRLRELSQQVARASGAPLPPWKAIVGDNAFAHESGIHAAGVLRDPQTYEPFPPEAVGSQRRLVLGKHS
GRHLLLQILKEQGIPVEQIPPGQLEKLLEQVRQHVTHCKHPIPPAELARYCRATLTQWAQHTPEPIPEESHPDVV

Sequences:

>Translated_395_residues
MNSSITINDTTLRDGEQAAGVAFQIPEKISIAALLDAIGVPEIEVGIPAMGAVETEAIRAMLSLGLRAHLLGWNRAVPSD
ILASIRCGLKRIHISVPVSDRQIAAKFQGDRPRLLRQLRQCVEMALEQGLWVSVGAEDASRTPLEQVIELAQMAQSWGCR
RFRYCDTVGVLDPFSTYKQIRRLVRAIDIPVEMHTHDDLGMATANALAGIRAGAQSVNTTVNGLGERAGNAPLEEVVMAL
RCTLGIDVGVDTRRLRELSQQVARASGAPLPPWKAIVGDNAFAHESGIHAAGVLRDPQTYEPFPPEAVGSQRRLVLGKHS
GRHLLLQILKEQGIPVEQIPPGQLEKLLEQVRQHVTHCKHPIPPAELARYCRATLTQWAQHTPEPIPEESHPDVV
>Mature_395_residues
MNSSITINDTTLRDGEQAAGVAFQIPEKISIAALLDAIGVPEIEVGIPAMGAVETEAIRAMLSLGLRAHLLGWNRAVPSD
ILASIRCGLKRIHISVPVSDRQIAAKFQGDRPRLLRQLRQCVEMALEQGLWVSVGAEDASRTPLEQVIELAQMAQSWGCR
RFRYCDTVGVLDPFSTYKQIRRLVRAIDIPVEMHTHDDLGMATANALAGIRAGAQSVNTTVNGLGERAGNAPLEEVVMAL
RCTLGIDVGVDTRRLRELSQQVARASGAPLPPWKAIVGDNAFAHESGIHAAGVLRDPQTYEPFPPEAVGSQRRLVLGKHS
GRHLLLQILKEQGIPVEQIPPGQLEKLLEQVRQHVTHCKHPIPPAELARYCRATLTQWAQHTPEPIPEESHPDVV

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=349, Percent_Identity=34.6704871060172, Blast_Score=186, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6320019, Length=308, Percent_Identity=29.2207792207792, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320071, Length=308, Percent_Identity=29.2207792207792, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324682, Length=322, Percent_Identity=25.776397515528, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 43141; Mature: 43141

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSITINDTTLRDGEQAAGVAFQIPEKISIAALLDAIGVPEIEVGIPAMGAVETEAIRA
CCCCEEEECCCCCCCHHHCCEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
MLSLGLRAHLLGWNRAVPSDILASIRCGLKRIHISVPVSDRQIAAKFQGDRPRLLRQLRQ
HHHHCHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHH
CVEMALEQGLWVSVGAEDASRTPLEQVIELAQMAQSWGCRRFRYCDTVGVLDPFSTYKQI
HHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHH
RRLVRAIDIPVEMHTHDDLGMATANALAGIRAGAQSVNTTVNGLGERAGNAPLEEVVMAL
HHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RCTLGIDVGVDTRRLRELSQQVARASGAPLPPWKAIVGDNAFAHESGIHAAGVLRDPQTY
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHCCCCCHHHCCCCCCCC
EPFPPEAVGSQRRLVLGKHSGRHLLLQILKEQGIPVEQIPPGQLEKLLEQVRQHVTHCKH
CCCCCHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PIPPAELARYCRATLTQWAQHTPEPIPEESHPDVV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSSITINDTTLRDGEQAAGVAFQIPEKISIAALLDAIGVPEIEVGIPAMGAVETEAIRA
CCCCEEEECCCCCCCHHHCCEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
MLSLGLRAHLLGWNRAVPSDILASIRCGLKRIHISVPVSDRQIAAKFQGDRPRLLRQLRQ
HHHHCHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHH
CVEMALEQGLWVSVGAEDASRTPLEQVIELAQMAQSWGCRRFRYCDTVGVLDPFSTYKQI
HHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHH
RRLVRAIDIPVEMHTHDDLGMATANALAGIRAGAQSVNTTVNGLGERAGNAPLEEVVMAL
HHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RCTLGIDVGVDTRRLRELSQQVARASGAPLPPWKAIVGDNAFAHESGIHAAGVLRDPQTY
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHCCCCCHHHCCCCCCCC
EPFPPEAVGSQRRLVLGKHSGRHLLLQILKEQGIPVEQIPPGQLEKLLEQVRQHVTHCKH
CCCCCHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PIPPAELARYCRATLTQWAQHTPEPIPEESHPDVV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9139910; 11759840 [H]