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The map label for this gene is nifS [H]

Identifier: 86607916

GI number: 86607916

Start: 427382

End: 428572

Strand: Direct

Name: nifS [H]

Synonym: CYB_0419

Alternate gene names: 86607916

Gene position: 427382-428572 (Clockwise)

Preceding gene: 86607915

Following gene: 86607917

Centisome position: 14.03

GC content: 63.48

Gene sequence:

>1191_bases
GTGATCTACCTGGACAACAACGCCACCACCGCCGTGGATCCCCTGGTTCTGGAGGCGATGCTGCCCTATTTGCAGACCTT
CTACGGCAACCCCTCCTCGATGCACAGTTTTGGCGGCCAGGTGGGGCGTGCCCTTAACCAAGCCCGCGAACAGGTGGCAG
CCCTTCTGGGAGCCGACCCGACGGAGATCGTCTTCACCAGTGGCGGCACCGAAGGCAACACCACGGCCATTTATGCAGCC
TTGGCAGCCCAACCCCAGAAGCGCCACATCATCACCACGCAGGTAGAGCACGCCTCGGTGCTCAACGTCTGCAAGCACCT
GGAAAAACAAGGCTACCGCGTCACCTACCTCTCGGTGGATGAGCAAGGCTGCCTGGACTTGCTGGAGTTGGAAGCAGCCC
TGACCGGCGATACTGCCCTGGTGAGCTGCATGGCCGCCAACAACGAGACGGGCGTGCTTTTCCCCATCGAGGCGGTGGGA
TCCCTGGCTCGCAGCTATGGGGCTGTTGTCCATGTGGACGCAGTACAGGCGGTGGGCAAGATCCCTCTCGATCTGCAGCG
CCTGCCGGTGGATCTCCTCACCCTTTCCGGGCACAAGCTGCATGCCCCCAAGGGGATCGGCGCCCTCTATGTGCGGCGGG
GCTTTCGCTTCCGTTCTCTGTTGCTGGGAGGACATCAAGAGCGGGGCCGCCGCGCCGGGACGGAAAATGTGCCGGGCATC
ATCGCCCTGGGCAAAGCGGCTGAGCTGGCCCAAGCCCACCTGGGGGATCCCCATGAGCGGCTGCTGCGGGATCGCTTGGA
GCAGGGCTTGCTACAGCGGATCCCGGATGTCCAGGTTAACGGCCACCCCACTCAGCGGCTGCCCAACACCACCAACTTGG
GCTTCAAGTTCGTGGAAGGGGAAGCGATCCTCTTCTGGCTGAACAAATACGGCATCTGCGCCTCCTCCGGCTCCGCCTGC
ACTTCCGGATCCCTGGAGCCCTCCCATGTGCTGCGGGCCATGGGCCTGCCCTACACCGTTTTGCACGGCTCAATCCGCTT
CAGCCTCTCTCGCTTTACCCAGCCGGCAGAGGTGGAGCGGGTGCTGGAGGTTTTGCCGGGGATCATTGAGCATTTGCGGG
CCATCTCCCCCTTCAGCCACGAGGCGGAAGACTGGCTGCAACAGCAGGAACGGGAACTGGCTCGCCGCTGA

Upstream 100 bases:

>100_bases
GCTTGCGCCCGCATTTGCCCAAAGCAGTGCCATTCCCACGCTCCGCAAGCCGTTTAACTTTGCCCAACCCCTATCTTGTA
CCGGAGCCTGTATGAACCCT

Downstream 100 bases:

>100_bases
CCCCCCTCAAACTCATCTTCCCTCGACCGTTAGCATCCTCAACTCAGGATCCAGCCATGTGGGACTACAGCGAAAAAGTT
TTGGAACTCTTCTACCACCC

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS2 [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADPTEIVFTSGGTEGNTTAIYAA
LAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVDEQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVG
SLARSYGAVVHVDAVQAVGKIPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGFRFRSLLLGGHQERGRRAGTENVPGI
IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEGEAILFWLNKYGICASSGSAC
TSGSLEPSHVLRAMGLPYTVLHGSIRFSLSRFTQPAEVERVLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR

Sequences:

>Translated_396_residues
MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADPTEIVFTSGGTEGNTTAIYAA
LAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVDEQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVG
SLARSYGAVVHVDAVQAVGKIPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGFRFRSLLLGGHQERGRRAGTENVPGI
IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEGEAILFWLNKYGICASSGSAC
TSGSLEPSHVLRAMGLPYTVLHGSIRFSLSRFTQPAEVERVLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR
>Mature_396_residues
MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADPTEIVFTSGGTEGNTTAIYAA
LAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVDEQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVG
SLARSYGAVVHVDAVQAVGKIPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGFRFRSLLLGGHQERGRRAGTENVPGI
IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEGEAILFWLNKYGICASSGSAC
TSGSLEPSHVLRAMGLPYTVLHGSIRFSLSRFTQPAEVERVLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=389, Percent_Identity=48.3290488431877, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=36.231884057971, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=48.5714285714286, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1787970, Length=389, Percent_Identity=27.5064267352185, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789175, Length=370, Percent_Identity=30.2702702702703, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=396, Percent_Identity=45.4545454545455, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17533177, Length=323, Percent_Identity=35.6037151702786, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319831, Length=395, Percent_Identity=44.8101265822785, Blast_Score=322, Evalue=9e-89,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=48.0519480519481, Blast_Score=338, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43067; Mature: 43067

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADP
CEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEIVFTSGGTEGNTTAIYAALAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVD
CEEEEECCCCCCCCCEEEEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEC
EQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVGSLARSYGAVVHVDAVQAVGK
CCHHHHHHHHHHHHCCCHHHHEEEECCCCCCEEEEHHHHHHHHHHCCCEEEEHHHHHHCC
IPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGFRFRSLLLGGHQERGRRAGTENVPGI
CCCCHHHCCEEEEEECCCEEECCCCCCHHHHHCCCHHHHHHHCCCHHHHCCCCCCCCCCE
IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEG
EEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCCHHCCCCCCCCCEEEECC
EAILFWLNKYGICASSGSACTSGSLEPSHVLRAMGLPYTVLHGSIRFSLSRFTQPAEVER
CEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHCCCHHHHHH
VLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADP
CEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEIVFTSGGTEGNTTAIYAALAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVD
CEEEEECCCCCCCCCEEEEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEC
EQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVGSLARSYGAVVHVDAVQAVGK
CCHHHHHHHHHHHHCCCHHHHEEEECCCCCCEEEEHHHHHHHHHHCCCEEEEHHHHHHCC
IPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGFRFRSLLLGGHQERGRRAGTENVPGI
CCCCHHHCCEEEEEECCCEEECCCCCCHHHHHCCCHHHHHHHCCCHHHHCCCCCCCCCCE
IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEG
EEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCCHHCCCCCCCCCEEEECC
EAILFWLNKYGICASSGSACTSGSLEPSHVLRAMGLPYTVLHGSIRFSLSRFTQPAEVER
CEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHCCCHHHHHH
VLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]