Click here to switch to the map view.

The map label for this gene is gldA [C]

Identifier: 86607836

GI number: 86607836

Start: 356719

End: 357846

Strand: Reverse

Name: gldA [C]

Synonym: CYB_0338

Alternate gene names: 86607836

Gene position: 357846-356719 (Counterclockwise)

Preceding gene: 86607837

Following gene: 86607832

Centisome position: 11.75

GC content: 64.1

Gene sequence:

>1128_bases
ATGCTAAGCGCCTCTCCTTGCCAATTGGATCTTGCCATTCCGGCCCCAGGTCGGCTGTACCGCGGCAGCGGCATTCTGGA
AGCTGTGCCCCTTGCGGTACTGGGATCCCGTGTGCTGGTGATCGGGGGGGAACGCTCCTTGGCTGTGGTTGAGTCCCCTC
TGCAGCGCAATTTCGCGCGGGATCCCGCCCTCCAAGTTCACTGGACTACCTACGGGGTAGATTGCAGCGAGTCGGAGCTG
GCGCGTTTGCAGGCGCTAGCCATGGCGGAGTCCACAACCGGCATCTTGGGGGTAGGCGGGGGCAAGGCTCTGGATACGGC
CAAGCTGGTGGCCCACCGGCTGCGGATCCCCGTGGTTACCGTGCCCACCTCTGCTGCCACCTGTGCCGCCTGGAGTGCCC
TATCCAATATCTACTCCAACAGCGGCGCTTTTCAGCGGGATGTGGCCTTGGATCAGGCCCCAGCCGCCTTGATCTTGGAC
TACGACCTCATTCGTCAGGCTCCCCCTCGCACCTTGGTAGCCGGGATCGGAGATGCCTTGGCTAAGTGGTACGAGTCTTC
CGTCAGCAGCGGCAGTTCAGAAGATGCCCTGGTGATTGGCGCTGTGCAGCAGGCCCGCGTCCTGCGGGATTTGCTCCTCC
AGCAATCGGCGGCGGCCTTGAGGGAGCCGGGCGGAACCGCTTGGAAGCAGGTGGTGGATGCCTGCATCTGCCTGGCGGGG
ATCGTCGGCGGCATGGGTGGGGCTAAGTGTCGCACCGTGGCCGCCCACGCTGTCCACAACGGGCTAACCCACTTGTCGGG
GCACCGGGCCACCCTGCACGGCGAGAAGGTGGCTTTCGGCATTTTGGCCCAGCTCCGCCTAGAAGAGATCCTGCAGGGAA
GCCAGTTGGCGCTGGCAGCACGTACCCAGTTGCTGGAGTTCTACCGCCAGGTGGGGCTGCCCCTCAACTTGCGGGATCTC
AAGCTAGGTCACTTGGGATCCGCCGACCTGCTGCATGCTGCCCAAGTGGCCTGCCAGCCAGGATCCGACATCCACCACCT
GCCCTTCCCCGTCAGTCCAGAAGCCCTGTTGGTGGCCCTTACCACCACAGACCTGACCAGCTCCAGCCCTGTTCTCCCTC
GGCTGTAA

Upstream 100 bases:

>100_bases
GGTGCGCAGCCCTTTTTGCGGCTAGGATCGTTATCTGTATTCACGGACACTGAGCTACCGCGATAGCGGGACGGAGTCCT
TTTTCTCGCCCATTCTTGCA

Downstream 100 bases:

>100_bases
GTCCTGGCAGAGGATATCTGTCTGCTATCCAACTCCGCCGGTACGGGCCTGGGTCGGCATTGTGGGCAGGCGGGCTTCGC
CGTAGGCTCCAGAGGTGGCG

Product: alcohol dehydrogenase, iron-containing

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MLSASPCQLDLAIPAPGRLYRGSGILEAVPLAVLGSRVLVIGGERSLAVVESPLQRNFARDPALQVHWTTYGVDCSESEL
ARLQALAMAESTTGILGVGGGKALDTAKLVAHRLRIPVVTVPTSAATCAAWSALSNIYSNSGAFQRDVALDQAPAALILD
YDLIRQAPPRTLVAGIGDALAKWYESSVSSGSSEDALVIGAVQQARVLRDLLLQQSAAALREPGGTAWKQVVDACICLAG
IVGGMGGAKCRTVAAHAVHNGLTHLSGHRATLHGEKVAFGILAQLRLEEILQGSQLALAARTQLLEFYRQVGLPLNLRDL
KLGHLGSADLLHAAQVACQPGSDIHHLPFPVSPEALLVALTTTDLTSSSPVLPRL

Sequences:

>Translated_375_residues
MLSASPCQLDLAIPAPGRLYRGSGILEAVPLAVLGSRVLVIGGERSLAVVESPLQRNFARDPALQVHWTTYGVDCSESEL
ARLQALAMAESTTGILGVGGGKALDTAKLVAHRLRIPVVTVPTSAATCAAWSALSNIYSNSGAFQRDVALDQAPAALILD
YDLIRQAPPRTLVAGIGDALAKWYESSVSSGSSEDALVIGAVQQARVLRDLLLQQSAAALREPGGTAWKQVVDACICLAG
IVGGMGGAKCRTVAAHAVHNGLTHLSGHRATLHGEKVAFGILAQLRLEEILQGSQLALAARTQLLEFYRQVGLPLNLRDL
KLGHLGSADLLHAAQVACQPGSDIHHLPFPVSPEALLVALTTTDLTSSSPVLPRL
>Mature_375_residues
MLSASPCQLDLAIPAPGRLYRGSGILEAVPLAVLGSRVLVIGGERSLAVVESPLQRNFARDPALQVHWTTYGVDCSESEL
ARLQALAMAESTTGILGVGGGKALDTAKLVAHRLRIPVVTVPTSAATCAAWSALSNIYSNSGAFQRDVALDQAPAALILD
YDLIRQAPPRTLVAGIGDALAKWYESSVSSGSSEDALVIGAVQQARVLRDLLLQQSAAALREPGGTAWKQVVDACICLAG
IVGGMGGAKCRTVAAHAVHNGLTHLSGHRATLHGEKVAFGILAQLRLEEILQGSQLALAARTQLLEFYRQVGLPLNLRDL
KLGHLGSADLLHAAQVACQPGSDIHHLPFPVSPEALLVALTTTDLTSSSPVLPRL

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=367, Percent_Identity=35.4223433242507, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1786815, Length=335, Percent_Identity=37.3134328358209, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 39236; Mature: 39236

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSASPCQLDLAIPAPGRLYRGSGILEAVPLAVLGSRVLVIGGERSLAVVESPLQRNFAR
CCCCCCCEEEEECCCCCCEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHHCHHHHHHCC
DPALQVHWTTYGVDCSESELARLQALAMAESTTGILGVGGGKALDTAKLVAHRLRIPVVT
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCEEE
VPTSAATCAAWSALSNIYSNSGAFQRDVALDQAPAALILDYDLIRQAPPRTLVAGIGDAL
ECCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEHHHHHCCCCHHHHHHHHHHH
AKWYESSVSSGSSEDALVIGAVQQARVLRDLLLQQSAAALREPGGTAWKQVVDACICLAG
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IVGGMGGAKCRTVAAHAVHNGLTHLSGHRATLHGEKVAFGILAQLRLEEILQGSQLALAA
HHCCCCCCHHHHHHHHHHHHCHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHH
RTQLLEFYRQVGLPLNLRDLKLGHLGSADLLHAAQVACQPGSDIHHLPFPVSPEALLVAL
HHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
TTTDLTSSSPVLPRL
EECCCCCCCCCCCCC
>Mature Secondary Structure
MLSASPCQLDLAIPAPGRLYRGSGILEAVPLAVLGSRVLVIGGERSLAVVESPLQRNFAR
CCCCCCCEEEEECCCCCCEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHHCHHHHHHCC
DPALQVHWTTYGVDCSESELARLQALAMAESTTGILGVGGGKALDTAKLVAHRLRIPVVT
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCEEE
VPTSAATCAAWSALSNIYSNSGAFQRDVALDQAPAALILDYDLIRQAPPRTLVAGIGDAL
ECCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEHHHHHCCCCHHHHHHHHHHH
AKWYESSVSSGSSEDALVIGAVQQARVLRDLLLQQSAAALREPGGTAWKQVVDACICLAG
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IVGGMGGAKCRTVAAHAVHNGLTHLSGHRATLHGEKVAFGILAQLRLEEILQGSQLALAA
HHCCCCCCHHHHHHHHHHHHCHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHH
RTQLLEFYRQVGLPLNLRDLKLGHLGSADLLHAAQVACQPGSDIHHLPFPVSPEALLVAL
HHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
TTTDLTSSSPVLPRL
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.5 {glycerol}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]