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The map label for this gene is thiC [H]

Identifier: 86607777

GI number: 86607777

Start: 292778

End: 294145

Strand: Direct

Name: thiC [H]

Synonym: CYB_0278

Alternate gene names: 86607777

Gene position: 292778-294145 (Clockwise)

Preceding gene: 86607776

Following gene: 86607778

Centisome position: 9.61

GC content: 54.46

Gene sequence:

>1368_bases
ATGCGCAGCGAATGGGTTGCCCGTCGCCGTGGGCAAGCAAATGTAACGCAGATGCACTTTGCCCGTCAGGGCGTGATTAC
CGAAGAGATGGAGTATGTGGCCCAGCGGGAAAACCTGCCGCCTGAACTGATCCGCTCGGAAGTGGCCCGGGGTCGCATGA
TCATCCCTGCCAACATCAATCACACCAACCTCGAGCCGATGTGCATTGGGATCGCTTCCCGGTGCAAAGTCAATGCCAAC
ATTGGCGCTTCCCCTACTTCTTCCGGCTTGGCCGAAGAGCTGGAAAAGCTGAAATTGGCCATCAAATACGGCGCCGATAC
CGTTATGGATCTGTCCACCGGCGGGGGCGATTTGGATGAAATTCGCACAGCCATTATCCAAGCTTCTCCAGTGCCCATTG
GCACGGTGCCCATCTACCAAGCCCTAGAAAGTGTGCACGGCAAGGTCGAAAAACTTTCTGCAGAAGACATCTTGCATGTT
ATCGAAAAACAGGCCCAGCAGGGGGTGGACTACATGACCATCCATGCCGGGATCCTGATCGAATATCTTCCGTTGGTACG
CCACCGCCTGACGGGAATTGTTTCTCGTGGGGGTGGGATCCTGGCCCGTTGGATGTTGGCCCACCACAAACAAAACCCGC
TTTATACCCACTTCCGCGACATTATCGAGATTTTCAAGAAATACGATGTTTCTTTTAGTCTGGGAGATTCTTTGCGACCG
GGATGTTTGCACGATGCCTCTGATGAAGCACAACTGGCGGAGTTGAAAACCCTGGGCCAGTTGACCCGCATGGCTTGGGA
GCACGATGTGCAGGTGATGGTGGAAGGGCCGGGGCATGTGCCGATGGATCAAATTGAATTCAACGTGCGCAAGCAAATGG
AAGAGTGCGATGAGGCTCCCTTCTATGTTTTGGGACCTCTGGTAACCGATATTGCGGCAGGCTATGACCACATCAGCTCC
GCCATTGGAGCAGCCTTAGCCGGTTGGTACGGGGCAGCCATGCTTTGCTATGTCACCCCCAAAGAGCACCTGGGTTTGCC
CAATGCAGAGGACGTGCGCAACGGCTTAATTGCCTATAAAATTGCCGCCCATGCTGCCGATATTGCCCGTCATCGCCCCG
GTGCCAGGGATCGCGATGATGAGATGTCGAGGGCCCGCTATAACTTCGACTGGAACCGGCAGTTTGAATTGTCTTTGGAT
CCAGAACGCGCCAGAGAATATCACGACGAAACCTTGCCGGCAGATATCTACAAAACGGCAGAGTTCTGCTCCATGTGTGG
GCCAAAGTTTTGCCCCATGCAAACCAAAATGGATGCAGAGGCTTTGAGTGAGCTGGAGCAGTTCTTGGCCGCGCAGCCAA
CAGGGTAA

Upstream 100 bases:

>100_bases
CGCTAGGAGTGTCTGGGGATTATCCCCAGGCTGAGATTACATCCTCAGAACCTGATCTGGTTCATACCAGCGTAGGAAAG
CGAGCACTGGAGTTGACAAC

Downstream 100 bases:

>100_bases
GCCTTGGCCCATCCCTGCCCTGAAGGCTTGAAAAAGGCCATCGTCTGTCTCGCCTATGGACTGGGTTGGGATCCCTGTCT
CAAGTTCTCAAGAAGTCAGG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MRSEWVARRRGQANVTQMHFARQGVITEEMEYVAQRENLPPELIRSEVARGRMIIPANINHTNLEPMCIGIASRCKVNAN
IGASPTSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDEIRTAIIQASPVPIGTVPIYQALESVHGKVEKLSAEDILHV
IEKQAQQGVDYMTIHAGILIEYLPLVRHRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDSLRP
GCLHDASDEAQLAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAPFYVLGPLVTDIAAGYDHISS
AIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLD
PERAREYHDETLPADIYKTAEFCSMCGPKFCPMQTKMDAEALSELEQFLAAQPTG

Sequences:

>Translated_455_residues
MRSEWVARRRGQANVTQMHFARQGVITEEMEYVAQRENLPPELIRSEVARGRMIIPANINHTNLEPMCIGIASRCKVNAN
IGASPTSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDEIRTAIIQASPVPIGTVPIYQALESVHGKVEKLSAEDILHV
IEKQAQQGVDYMTIHAGILIEYLPLVRHRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDSLRP
GCLHDASDEAQLAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAPFYVLGPLVTDIAAGYDHISS
AIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLD
PERAREYHDETLPADIYKTAEFCSMCGPKFCPMQTKMDAEALSELEQFLAAQPTG
>Mature_455_residues
MRSEWVARRRGQANVTQMHFARQGVITEEMEYVAQRENLPPELIRSEVARGRMIIPANINHTNLEPMCIGIASRCKVNAN
IGASPTSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDEIRTAIIQASPVPIGTVPIYQALESVHGKVEKLSAEDILHV
IEKQAQQGVDYMTIHAGILIEYLPLVRHRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDSLRP
GCLHDASDEAQLAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAPFYVLGPLVTDIAAGYDHISS
AIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLD
PERAREYHDETLPADIYKTAEFCSMCGPKFCPMQTKMDAEALSELEQFLAAQPTG

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=458, Percent_Identity=58.9519650655022, Blast_Score=551, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 50660; Mature: 50660

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSEWVARRRGQANVTQMHFARQGVITEEMEYVAQRENLPPELIRSEVARGRMIIPANIN
CCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HTNLEPMCIGIASRCKVNANIGASPTSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDE
CCCCCHHEEEHHHHCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
IRTAIIQASPVPIGTVPIYQALESVHGKVEKLSAEDILHVIEKQAQQGVDYMTIHAGILI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEHHHHHHH
EYLPLVRHRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDSLRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCC
GCLHDASDEAQLAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDIAAGYDHISSAIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYK
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCHHHHH
IAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLDPERAREYHDETLPADIYKTA
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKFCPMQTKMDAEALSELEQFLAAQPTG
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSEWVARRRGQANVTQMHFARQGVITEEMEYVAQRENLPPELIRSEVARGRMIIPANIN
CCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HTNLEPMCIGIASRCKVNANIGASPTSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDE
CCCCCHHEEEHHHHCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
IRTAIIQASPVPIGTVPIYQALESVHGKVEKLSAEDILHVIEKQAQQGVDYMTIHAGILI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEHHHHHHH
EYLPLVRHRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDSLRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCC
GCLHDASDEAQLAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDIAAGYDHISSAIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYK
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCHHHHH
IAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLDPERAREYHDETLPADIYKTA
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKFCPMQTKMDAEALSELEQFLAAQPTG
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA