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The map label for this gene is phnD-1 [H]

Identifier: 86607662

GI number: 86607662

Start: 173747

End: 174643

Strand: Direct

Name: phnD-1 [H]

Synonym: CYB_0160

Alternate gene names: 86607662

Gene position: 173747-174643 (Clockwise)

Preceding gene: 86607661

Following gene: 86607663

Centisome position: 5.7

GC content: 53.96

Gene sequence:

>897_bases
ATGCGGAGAAGAAAAATTCTGTTCCTTGCTGCCTTCCTCATGGGAATTTCCAGCTGGGTTCCAGGGCTGGCTCAGAAGCA
AAACTGGCCCAAGGAGCTGGTGATTGCTGAAGTACCGGTGGAGAGTGCTGCCGATTACGAAGCTCGCTATGCTCCCTTTT
TGGATTATCTCAAACAAAAACTGGGGATCCCCGTCCGCCTGTTTGTGGCCGGTGACTACACGGCGGTGATGGTCTCCCAG
GCACAAGGCCAAACTCACGTAGCTTTCTACGGCCCTGGCTCCTACGTAGATGCCATTGAAAAGGCCAACGCTCCCATTGA
AGCGTTCGTGAAAGAGGACTCGCTGCGCTCGGGTACCGTTTATCACTCCTTGATACTTTCCAAGAAAGGCAGTGGCATCA
AAACTCTAGAAGACGCCAAGGGCAAAGATTTTGCTTTCGTCGACCCAGAGAGCACCTCTGGCTTCAAGGTGCCCATGGCC
TACTTCTGTCTTGAGGCCAAAATCAAGCCCAAGGAGTATTTCAAGCGGGTGGCTTTTGCTGGCACCCACGAGTCGGTCAT
CTTGGGGATAGCTCAGGGAACCATTCCCGTTGGTGTAACCTGGGATACCGGGATCAGCATTGCCACCAACAAGGGGCAGA
TCAAAGGCATAGAGGACCTTGAGGTCATCTGGAGATCGGATCCCATTCCTTCCTCTCCCTGGGCCTACCGTAAAGATCTG
CCAGAGGATCTCAAGGAGGCCTTGCGCACGGCCTATCTGGAGTACGAAAACACGCCGGAGGGTCAGAAATGGCTGGAGGC
TCGTGGACTGAAGGGCTTTGAGCCGGCTAGCGACGCAGATTACGATCCCTTCCGCAAGGTCAACGATGCTCTTAAGAAAC
CTGAGTGCCAACTCTAG

Upstream 100 bases:

>100_bases
GAGAAGGAAACCCACGAGGCAAGCGAATGGTTTTCCTCAACCTTGATGGTCTAACAAGATCCACCTTCAACCGCAAGTTA
ACTGCAAAGGAGAAAACCTT

Downstream 100 bases:

>100_bases
GAGACTTCCCCCCCTGCCAGCAGAGCTTAAGCGCAGGGAGCTGATCACGGTTGTGCTGTATGGGGGGGTTTTTCTCGCTC
TCTTGCTGACGGCGGAGCCT

Product: phosphonate ABC tranporter, periplasmic phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MRRRKILFLAAFLMGISSWVPGLAQKQNWPKELVIAEVPVESAADYEARYAPFLDYLKQKLGIPVRLFVAGDYTAVMVSQ
AQGQTHVAFYGPGSYVDAIEKANAPIEAFVKEDSLRSGTVYHSLILSKKGSGIKTLEDAKGKDFAFVDPESTSGFKVPMA
YFCLEAKIKPKEYFKRVAFAGTHESVILGIAQGTIPVGVTWDTGISIATNKGQIKGIEDLEVIWRSDPIPSSPWAYRKDL
PEDLKEALRTAYLEYENTPEGQKWLEARGLKGFEPASDADYDPFRKVNDALKKPECQL

Sequences:

>Translated_298_residues
MRRRKILFLAAFLMGISSWVPGLAQKQNWPKELVIAEVPVESAADYEARYAPFLDYLKQKLGIPVRLFVAGDYTAVMVSQ
AQGQTHVAFYGPGSYVDAIEKANAPIEAFVKEDSLRSGTVYHSLILSKKGSGIKTLEDAKGKDFAFVDPESTSGFKVPMA
YFCLEAKIKPKEYFKRVAFAGTHESVILGIAQGTIPVGVTWDTGISIATNKGQIKGIEDLEVIWRSDPIPSSPWAYRKDL
PEDLKEALRTAYLEYENTPEGQKWLEARGLKGFEPASDADYDPFRKVNDALKKPECQL
>Mature_298_residues
MRRRKILFLAAFLMGISSWVPGLAQKQNWPKELVIAEVPVESAADYEARYAPFLDYLKQKLGIPVRLFVAGDYTAVMVSQ
AQGQTHVAFYGPGSYVDAIEKANAPIEAFVKEDSLRSGTVYHSLILSKKGSGIKTLEDAKGKDFAFVDPESTSGFKVPMA
YFCLEAKIKPKEYFKRVAFAGTHESVILGIAQGTIPVGVTWDTGISIATNKGQIKGIEDLEVIWRSDPIPSSPWAYRKDL
PEDLKEALRTAYLEYENTPEGQKWLEARGLKGFEPASDADYDPFRKVNDALKKPECQL

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for phosphate binding [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790543, Length=271, Percent_Identity=31.3653136531365, Blast_Score=118, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 33135; Mature: 33135

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRKILFLAAFLMGISSWVPGLAQKQNWPKELVIAEVPVESAADYEARYAPFLDYLKQK
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHH
LGIPVRLFVAGDYTAVMVSQAQGQTHVAFYGPGSYVDAIEKANAPIEAFVKEDSLRSGTV
HCCCEEEEEECCEEEEEEECCCCCEEEEEECCCHHHHHHHHCCCCCEEHHHHCCCCCCHH
YHSLILSKKGSGIKTLEDAKGKDFAFVDPESTSGFKVPMAYFCLEAKIKPKEYFKRVAFA
HHHHHHHCCCCCCCCHHCCCCCCEEEECCCCCCCCCCCHHHHEECCCCCHHHHHHHHHHC
GTHESVILGIAQGTIPVGVTWDTGISIATNKGQIKGIEDLEVIWRSDPIPSSPWAYRKDL
CCCCEEEEEEECCCEEEEEEECCCEEEEECCCCCCCCHHHHHEECCCCCCCCCCHHHHCC
PEDLKEALRTAYLEYENTPEGQKWLEARGLKGFEPASDADYDPFRKVNDALKKPECQL
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRRRKILFLAAFLMGISSWVPGLAQKQNWPKELVIAEVPVESAADYEARYAPFLDYLKQK
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHH
LGIPVRLFVAGDYTAVMVSQAQGQTHVAFYGPGSYVDAIEKANAPIEAFVKEDSLRSGTV
HCCCEEEEEECCEEEEEEECCCCCEEEEEECCCHHHHHHHHCCCCCEEHHHHCCCCCCHH
YHSLILSKKGSGIKTLEDAKGKDFAFVDPESTSGFKVPMAYFCLEAKIKPKEYFKRVAFA
HHHHHHHCCCCCCCCHHCCCCCCEEEECCCCCCCCCCCHHHHEECCCCCHHHHHHHHHHC
GTHESVILGIAQGTIPVGVTWDTGISIATNKGQIKGIEDLEVIWRSDPIPSSPWAYRKDL
CCCCEEEEEEECCCEEEEEEECCCEEEEECCCCCCCCHHHHHEECCCCCCCCCCHHHHCC
PEDLKEALRTAYLEYENTPEGQKWLEARGLKGFEPASDADYDPFRKVNDALKKPECQL
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA