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The map label for this gene is gloB [H]

Identifier: 86607653

GI number: 86607653

Start: 166156

End: 166914

Strand: Direct

Name: gloB [H]

Synonym: CYB_0151

Alternate gene names: 86607653

Gene position: 166156-166914 (Clockwise)

Preceding gene: 86607652

Following gene: 86607661

Centisome position: 5.45

GC content: 62.06

Gene sequence:

>759_bases
ATGCGCGTTCTCCAGTTGCCTGCCCTGCGCGATAACTACATCTACCTGCTCCACAATCCCGACACTGCAACAGCAGCCGT
GGTGGATCCGGCAGTGGCTGAGCCCGTGTTGGAAAAGCTGGCCGAGCTGGGGGCGGAGTTGGTGGCCATTTTCAACACCC
ACCACCACCACGACCACGTGGGGGGCAACCGACAGCTTTTGGAGCGCTATCCCCGTGCCAGAGTCTATGGCAGCCAGGTG
GATCGGGGGCGGATCCCTGGCCAGACGGTGGAGCTCAAGGCAGGGGAAACAGTGGAGTTTGCGGGTCGGCTAGCAAAGGT
GCTGTTTGTGCCCGGCCATACCCGCGGCCATATTGCCTACTACTTTTCCGAGAGTGGGGATCTCTTCTGTGGCGATACCC
TGTTTGCCGGGGGATGCGGGCGGCTGTTTGAAGGCACGCCGGAGCAGATGGTGGGATCCCTGGATCAACTGCGGCAGTTG
CCGGAAGCCACACGGGTGTGGTGTGCCCACGAATACACCTTGAACAATCTCAGGTTTGCCCTGACAGTGGATGGAGACAA
CCCCGACCTGCAGGCGCGTTATCAGCAGGTGGCTGCCCTGCGCCAGACGGGATCCCCGACTGTCCCCAGCACCATTGGCG
AGGAACGCCGCACCAATCCCTTCTTGCGTTGGGATCAGCCGGCTTTGCAGGCAGCCACCGGGCTCCAGGATCCGGTGCGG
GTCTTCGCTCGCCTGCGCGGTATGAAGGATCAGTTCTGA

Upstream 100 bases:

>100_bases
CGATACACCTACCCAGACGGCAACACGAAAACTTTATCTTTTCCCTATTCCCTCAGCCGGCAAGACGTAGAACAGGCCAT
TTTTGAGAGTTAGCCGAGCC

Downstream 100 bases:

>100_bases
AAATCGGGTTCTGACAAAAGCTCCTGGAGGTGGCCCAAAACCTGAGGGGATGGTGCTCCTCACCACTAGCACAAACGAAA
CCCCCGAAGAGCTGCACCGT

Product: glyoxalase II family protein

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRVLQLPALRDNYIYLLHNPDTATAAVVDPAVAEPVLEKLAELGAELVAIFNTHHHHDHVGGNRQLLERYPRARVYGSQV
DRGRIPGQTVELKAGETVEFAGRLAKVLFVPGHTRGHIAYYFSESGDLFCGDTLFAGGCGRLFEGTPEQMVGSLDQLRQL
PEATRVWCAHEYTLNNLRFALTVDGDNPDLQARYQQVAALRQTGSPTVPSTIGEERRTNPFLRWDQPALQAATGLQDPVR
VFARLRGMKDQF

Sequences:

>Translated_252_residues
MRVLQLPALRDNYIYLLHNPDTATAAVVDPAVAEPVLEKLAELGAELVAIFNTHHHHDHVGGNRQLLERYPRARVYGSQV
DRGRIPGQTVELKAGETVEFAGRLAKVLFVPGHTRGHIAYYFSESGDLFCGDTLFAGGCGRLFEGTPEQMVGSLDQLRQL
PEATRVWCAHEYTLNNLRFALTVDGDNPDLQARYQQVAALRQTGSPTVPSTIGEERRTNPFLRWDQPALQAATGLQDPVR
VFARLRGMKDQF
>Mature_252_residues
MRVLQLPALRDNYIYLLHNPDTATAAVVDPAVAEPVLEKLAELGAELVAIFNTHHHHDHVGGNRQLLERYPRARVYGSQV
DRGRIPGQTVELKAGETVEFAGRLAKVLFVPGHTRGHIAYYFSESGDLFCGDTLFAGGCGRLFEGTPEQMVGSLDQLRQL
PEATRVWCAHEYTLNNLRFALTVDGDNPDLQARYQQVAALRQTGSPTVPSTIGEERRTNPFLRWDQPALQAATGLQDPVR
VFARLRGMKDQF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=258, Percent_Identity=39.922480620155, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI94538322, Length=258, Percent_Identity=39.922480620155, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI14150041, Length=257, Percent_Identity=38.9105058365759, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI116642887, Length=239, Percent_Identity=41.8410041841004, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI21703352, Length=239, Percent_Identity=41.8410041841004, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI46361987, Length=204, Percent_Identity=39.2156862745098, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI41327741, Length=169, Percent_Identity=29.585798816568, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786406, Length=256, Percent_Identity=44.921875, Blast_Score=190, Evalue=8e-50,
Organism=Escherichia coli, GI1787158, Length=175, Percent_Identity=28, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=252, Percent_Identity=37.6984126984127, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17538952, Length=165, Percent_Identity=29.0909090909091, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320478, Length=265, Percent_Identity=32.4528301886792, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324614, Length=245, Percent_Identity=33.8775510204082, Blast_Score=99, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=44.2307692307692, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=44.2307692307692, Blast_Score=206, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=44.2307692307692, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI221330176, Length=169, Percent_Identity=31.9526627218935, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28043; Mature: 28043

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLQLPALRDNYIYLLHNPDTATAAVVDPAVAEPVLEKLAELGAELVAIFNTHHHHDHV
CCEEECCCCCCCEEEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGNRQLLERYPRARVYGSQVDRGRIPGQTVELKAGETVEFAGRLAKVLFVPGHTRGHIAY
CCCHHHHHHCCCHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHEEEEECCCCCCCEEEE
YFSESGDLFCGDTLFAGGCGRLFEGTPEQMVGSLDQLRQLPEATRVWCAHEYTLNNLRFA
EEECCCCEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHCHHHHHHHHHEEEEECCEEEE
LTVDGDNPDLQARYQQVAALRQTGSPTVPSTIGEERRTNPFLRWDQPALQAATGLQDPVR
EEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHCHHHCCCCCEECCCHHHHHHHCCCHHHH
VFARLRGMKDQF
HHHHHHCCCCCC
>Mature Secondary Structure
MRVLQLPALRDNYIYLLHNPDTATAAVVDPAVAEPVLEKLAELGAELVAIFNTHHHHDHV
CCEEECCCCCCCEEEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGNRQLLERYPRARVYGSQVDRGRIPGQTVELKAGETVEFAGRLAKVLFVPGHTRGHIAY
CCCHHHHHHCCCHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHEEEEECCCCCCCEEEE
YFSESGDLFCGDTLFAGGCGRLFEGTPEQMVGSLDQLRQLPEATRVWCAHEYTLNNLRFA
EEECCCCEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHCHHHHHHHHHEEEEECCEEEE
LTVDGDNPDLQARYQQVAALRQTGSPTVPSTIGEERRTNPFLRWDQPALQAATGLQDPVR
EEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHCHHHCCCCCEECCCHHHHHHHCCCHHHH
VFARLRGMKDQF
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA