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The map label for this gene is ispG [H]

Identifier: 86607623

GI number: 86607623

Start: 133905

End: 135134

Strand: Direct

Name: ispG [H]

Synonym: CYB_0121

Alternate gene names: 86607623

Gene position: 133905-135134 (Clockwise)

Preceding gene: 86607622

Following gene: 86607625

Centisome position: 4.4

GC content: 60.16

Gene sequence:

>1230_bases
ATGCAAACCCTGGATCGGCCCAACGCCCCCACCCAGCAGCCCTACCCAGAACCGGTCTATCCCCGTCGCCCCACCCGTAC
GGTGGCCGTAGCCGATGTGTTGATCGGCAGCCAGCACCCGGTGGTGGTACAGTCCATGATTAACGAGGACACCCTCGACG
TCGACGCGGCAGTGGCCGGGATCCGCCGTCTGCACGAGGCGGGCTCAGAGATTGTGCGGGTTACCGTTCCCAGCATGGCC
CATGCCAAGGCGATGGAGGCAATTCGGGAGAAGCTGATCCAAACCTACAAGCCGGTGCCTTTGGTAGCCGATGTCCATCA
CAACGGCATCAAGATCGCCCTGGAGGTGGCCAAATACGTGGACAAGGTGCGCATCAATCCAGGGTTGTTCGTCTTTGAAA
AACCTCAGCCCGGTCGCACAGAATATACCCAGGCGGAATTTGAGGCCATTCGTAACAAAATCCGGGAAACCTTCACCCCC
CTTGTCCAGACCCTCAAAGCCCAGAACAAAGCCCTGCGCATCGGGGTAAATCATGGATCCCTGGCAGAGCGGATGCTCTT
CACCTACGGGGATACGCCTGAGGGCATGGTGGAGTCGGCCCTGGAATTTGCCCAAATCTGCGCCGAGCAAGACTTTCACA
ATGTGGTGCTTTCCTTCAAGGCCTCCCGCCCGCAGGTCATGTTGGCGGCCTACCGGCTGGCAGCCCGTCGCTTCGATGCC
CTGGGCCTGAATTACCCCTTCCACTTAGGTGTCACCGAGGCGGGCGATGGAGAATATGGGCGCATCAAGTCGGCGGTGGG
GATCGGAACCCTCTTGGCTGAAGGGATCGGCGACACGATTCGGGTTTCTCTCACGGAAGCGCCAGAGAAGGAGATCCCGG
TGGCCTACGGCATTTTGCAAGCTCTGAACCTGCGCAAAACCATGGTGGAGTATGTGGCTTGCCCTTCCTGTGGCCGCACC
CTCTTCAATTTGGAGGAGGTGCTGCAGAAGGTGCGCGCCGCAACCCAGCACCTGGTGGGGCTGGACATTGCCGTCATGGG
CTGCATTGTCAATGGGCCGGGAGAGATGGCCGACGCCGACTACGGCTACGTGGGCAAGACGCCGGGGGTGATCTCTCTCT
ACCGCGGCAAAGAGGAAGTGAAAAGGGTGCCGGAATCGGAAGGCGTGCAGGCGCTGATCGATCTGATCAAAGCCGATGGG
CGCTGGGTGGATCCCCCTTCTTCCCATTAA

Upstream 100 bases:

>100_bases
GGGGTTGAACCCCTCAGCCTCTACCCTGAGCTGAGTCGAATCCCAGGTTAGTTTCCGACCTACAATCAAGCTAGAGCAGC
TCTTTTGGCTTGACCAAGCT

Downstream 100 bases:

>100_bases
CTCTGCTTCCCCCTCAAGAGGCGACCTGCCCAGCCATCCTGCCGCGCTCTTGCTTCGCTCAGAAACTGGCTAGTGGCGTT
TGCCACGCAGGGCTTTGGCG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MQTLDRPNAPTQQPYPEPVYPRRPTRTVAVADVLIGSQHPVVVQSMINEDTLDVDAAVAGIRRLHEAGSEIVRVTVPSMA
HAKAMEAIREKLIQTYKPVPLVADVHHNGIKIALEVAKYVDKVRINPGLFVFEKPQPGRTEYTQAEFEAIRNKIRETFTP
LVQTLKAQNKALRIGVNHGSLAERMLFTYGDTPEGMVESALEFAQICAEQDFHNVVLSFKASRPQVMLAAYRLAARRFDA
LGLNYPFHLGVTEAGDGEYGRIKSAVGIGTLLAEGIGDTIRVSLTEAPEKEIPVAYGILQALNLRKTMVEYVACPSCGRT
LFNLEEVLQKVRAATQHLVGLDIAVMGCIVNGPGEMADADYGYVGKTPGVISLYRGKEEVKRVPESEGVQALIDLIKADG
RWVDPPSSH

Sequences:

>Translated_409_residues
MQTLDRPNAPTQQPYPEPVYPRRPTRTVAVADVLIGSQHPVVVQSMINEDTLDVDAAVAGIRRLHEAGSEIVRVTVPSMA
HAKAMEAIREKLIQTYKPVPLVADVHHNGIKIALEVAKYVDKVRINPGLFVFEKPQPGRTEYTQAEFEAIRNKIRETFTP
LVQTLKAQNKALRIGVNHGSLAERMLFTYGDTPEGMVESALEFAQICAEQDFHNVVLSFKASRPQVMLAAYRLAARRFDA
LGLNYPFHLGVTEAGDGEYGRIKSAVGIGTLLAEGIGDTIRVSLTEAPEKEIPVAYGILQALNLRKTMVEYVACPSCGRT
LFNLEEVLQKVRAATQHLVGLDIAVMGCIVNGPGEMADADYGYVGKTPGVISLYRGKEEVKRVPESEGVQALIDLIKADG
RWVDPPSSH
>Mature_409_residues
MQTLDRPNAPTQQPYPEPVYPRRPTRTVAVADVLIGSQHPVVVQSMINEDTLDVDAAVAGIRRLHEAGSEIVRVTVPSMA
HAKAMEAIREKLIQTYKPVPLVADVHHNGIKIALEVAKYVDKVRINPGLFVFEKPQPGRTEYTQAEFEAIRNKIRETFTP
LVQTLKAQNKALRIGVNHGSLAERMLFTYGDTPEGMVESALEFAQICAEQDFHNVVLSFKASRPQVMLAAYRLAARRFDA
LGLNYPFHLGVTEAGDGEYGRIKSAVGIGTLLAEGIGDTIRVSLTEAPEKEIPVAYGILQALNLRKTMVEYVACPSCGRT
LFNLEEVLQKVRAATQHLVGLDIAVMGCIVNGPGEMADADYGYVGKTPGVISLYRGKEEVKRVPESEGVQALIDLIKADG
RWVDPPSSH

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=395, Percent_Identity=37.7215189873418, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44858; Mature: 44858

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLDRPNAPTQQPYPEPVYPRRPTRTVAVADVLIGSQHPVVVQSMINEDTLDVDAAVAG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHH
IRRLHEAGSEIVRVTVPSMAHAKAMEAIREKLIQTYKPVPLVADVHHNGIKIALEVAKYV
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
DKVRINPGLFVFEKPQPGRTEYTQAEFEAIRNKIRETFTPLVQTLKAQNKALRIGVNHGS
HHEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCH
LAERMLFTYGDTPEGMVESALEFAQICAEQDFHNVVLSFKASRPQVMLAAYRLAARRFDA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCHHHHHHHHHHHHHHHH
LGLNYPFHLGVTEAGDGEYGRIKSAVGIGTLLAEGIGDTIRVSLTEAPEKEIPVAYGILQ
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
ALNLRKTMVEYVACPSCGRTLFNLEEVLQKVRAATQHLVGLDIAVMGCIVNGPGEMADAD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
YGYVGKTPGVISLYRGKEEVKRVPESEGVQALIDLIKADGRWVDPPSSH
CCCCCCCCCEEEEECCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQTLDRPNAPTQQPYPEPVYPRRPTRTVAVADVLIGSQHPVVVQSMINEDTLDVDAAVAG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHH
IRRLHEAGSEIVRVTVPSMAHAKAMEAIREKLIQTYKPVPLVADVHHNGIKIALEVAKYV
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
DKVRINPGLFVFEKPQPGRTEYTQAEFEAIRNKIRETFTPLVQTLKAQNKALRIGVNHGS
HHEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCH
LAERMLFTYGDTPEGMVESALEFAQICAEQDFHNVVLSFKASRPQVMLAAYRLAARRFDA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCHHHHHHHHHHHHHHHH
LGLNYPFHLGVTEAGDGEYGRIKSAVGIGTLLAEGIGDTIRVSLTEAPEKEIPVAYGILQ
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
ALNLRKTMVEYVACPSCGRTLFNLEEVLQKVRAATQHLVGLDIAVMGCIVNGPGEMADAD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
YGYVGKTPGVISLYRGKEEVKRVPESEGVQALIDLIKADGRWVDPPSSH
CCCCCCCCCEEEEECCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA