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The map label for this gene is miaE

Identifier: 86606825

GI number: 86606825

Start: 2204738

End: 2205343

Strand: Reverse

Name: miaE

Synonym: CYA_2185

Alternate gene names: 86606825

Gene position: 2205343-2204738 (Counterclockwise)

Preceding gene: 86606826

Following gene: 86606824

Centisome position: 75.2

GC content: 64.36

Gene sequence:

>606_bases
ATGCCTGAAACCTCTTCTGACCCTGTGCCGACGCTACAACTGGCCTCGCCGACTTCTGCGGCCTGGATCGACCAAGCCAT
TCGTCACCTCGACCTTATCTTGTTGGATCACGCCAACTGCGAGAAAAAAGCGGCGGGCAACGCCCTTAGTTTGCTGTTTC
GCTACCCGGCCAACGGGCGGCTGGTGGAAGCGCTGTCGCCGCTGGCGCGGGAGGAGCTGCAGCACTTTGAGCGGGTGCAT
CGCCACCTGCAACGGCTGGGGATCCCGATCAGGCCCCTCTCGGCTGCTCCCTACGCCAGCCGTCTCAACCAGCACCTGCG
CCGCTCCGAGCCGGAGCACCTTTTGGACGCTTTGCTGGTGGCCGCCATCATCGAGGCCCGCAGCCACGAACGCCTGGGCC
TGCTGGGGATCCACGCCCCCGACCCGGAGCTGCGGCAGTTTTACCTCTGGCTGGCGGCGGCTGAAGAGCGGCACTGGCAG
TTGTATCTAGACCTGGCGCTGGAGCAGTTCCCCGCTGACGAGGTGCAGGAGCGGCTGGACACCCTGCTGCAGGTGGAAGC
GGAGATTTTATCGACCCTTCACCCTGAGCCTCGTGTCCACAGCTAA

Upstream 100 bases:

>100_bases
AGACTTAGAAAGGGGTACGCCGGCAAAGCTAAGATCCGATTAACCCAGTTCCGTTCCCTGCTCCCGCAGCGGGCAGGGTG
GGTCAACCTGGAAGCTGTTG

Downstream 100 bases:

>100_bases
CCCATCCCCTTCCCGCGCAACCCCCATGTTCCAGGCAACGCGGCGGTGGTTGGCCCTCTGGTACACCGTGGTAACAGGGG
TGTTTTTGTTGCTGTTTGCC

Product: tRNA-(MS[2]IO[6]A)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MPETSSDPVPTLQLASPTSAAWIDQAIRHLDLILLDHANCEKKAAGNALSLLFRYPANGRLVEALSPLAREELQHFERVH
RHLQRLGIPIRPLSAAPYASRLNQHLRRSEPEHLLDALLVAAIIEARSHERLGLLGIHAPDPELRQFYLWLAAAEERHWQ
LYLDLALEQFPADEVQERLDTLLQVEAEILSTLHPEPRVHS

Sequences:

>Translated_201_residues
MPETSSDPVPTLQLASPTSAAWIDQAIRHLDLILLDHANCEKKAAGNALSLLFRYPANGRLVEALSPLAREELQHFERVH
RHLQRLGIPIRPLSAAPYASRLNQHLRRSEPEHLLDALLVAAIIEARSHERLGLLGIHAPDPELRQFYLWLAAAEERHWQ
LYLDLALEQFPADEVQERLDTLLQVEAEILSTLHPEPRVHS
>Mature_200_residues
PETSSDPVPTLQLASPTSAAWIDQAIRHLDLILLDHANCEKKAAGNALSLLFRYPANGRLVEALSPLAREELQHFERVHR
HLQRLGIPIRPLSAAPYASRLNQHLRRSEPEHLLDALLVAAIIEARSHERLGLLGIHAPDPELRQFYLWLAAAEERHWQL
YLDLALEQFPADEVQERLDTLLQVEAEILSTLHPEPRVHS

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 22805; Mature: 22674

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETSSDPVPTLQLASPTSAAWIDQAIRHLDLILLDHANCEKKAAGNALSLLFRYPANGR
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHEECCCCCH
LVEALSPLAREELQHFERVHRHLQRLGIPIRPLSAAPYASRLNQHLRRSEPEHLLDALLV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
AAIIEARSHERLGLLGIHAPDPELRQFYLWLAAAEERHWQLYLDLALEQFPADEVQERLD
HHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHH
TLLQVEAEILSTLHPEPRVHS
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PETSSDPVPTLQLASPTSAAWIDQAIRHLDLILLDHANCEKKAAGNALSLLFRYPANGR
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHEECCCCCH
LVEALSPLAREELQHFERVHRHLQRLGIPIRPLSAAPYASRLNQHLRRSEPEHLLDALLV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
AAIIEARSHERLGLLGIHAPDPELRQFYLWLAAAEERHWQLYLDLALEQFPADEVQERLD
HHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHH
TLLQVEAEILSTLHPEPRVHS
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]