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The map label for this gene is aspA

Identifier: 86606782

GI number: 86606782

Start: 2154964

End: 2156349

Strand: Reverse

Name: aspA

Synonym: CYA_2142

Alternate gene names: 86606782

Gene position: 2156349-2154964 (Counterclockwise)

Preceding gene: 86606783

Following gene: 86606781

Centisome position: 73.53

GC content: 63.64

Gene sequence:

>1386_bases
GTGCGCAGCGAGCGGGACGCGATGGGGGAGCGGCAACTGCCGCAGTCGGTGTACTACGGCATCCAAACCGCCCGCGCCCT
GGAGAATTTTCCCATCAGCGGCCTCAAGCCGCTGCCGGAGTACGTGGAGGCCTGCGTGCTCATCAAAAAAGCGGCGGCGG
AGGTCAACGGCGAGCTGGGCTGCATTCCCCCCGACGTGGCCAAGGCCATTGTCCAAGCGGCAGACGAGATCCTCCAGGGA
TCCCTGCGGGATCAGTTTGTGGTGGATGTGTACCAGGCCGGGGCGGGCACCTCCCACCACATGAACGTCAATGAGGTGTT
GGCCAACCGCGCCCTGGAGATCCTGGGAGAGCCCAAGGGGCGCTACGAGCGGGTCAGCCCCAACGACCATGTCAACTACG
GCCAATCCACCAACGACGTCATCCCCACCGCCATTCGCCTGGCGGCCCTGCTGCGGGTGCGGCGGGATCTTTTGGGCACG
CTGGACAACCTGGTGGCGGCGCTGGAGCAGAAAGCACAGGAGTTCCACGGCATTCTCAAGGCGGGCCGCACCCATTTGCA
GGATGCTGTCCCCATAAGGCTGGGAGATAGCTTCGGCGCCTATGCGGCCATCTTTGCCCAGCACCGCCGCTGGATCGAGG
AGGCCAGCCAGGCTCTGGGCAGCTTGGGGCTGGGGGGCAGCGCCGTCGGCACCGGCCTCAATACCCATCCCCAGTACCGG
CAAAAGGTGGTGCAACGGCTGGCAGAGTACACCCAGTTGCCCCTTACCCCGGCCCCCCATCCCATGGCGGCGATGCAGAG
CATGGCCCCCTTTGTGCACCTGTCGGGATCCCTGCGCAACCTGGCCCAAGATGCCATCAAAATGGCCAACGACCTACGCC
TGATGGATTCCGGCCCCAAGACTGGCCTCAAAGAGATCCAACTGCCGCCGGTACAGCCCGGCTCCTCCATCATGCCCGGC
AAGTACAACCCGGTGATGGCCGAGATGCTGAACATGGTCTGCTTTCAGGTGATCGGCCTGGACACCGCCATTCTCCTCGC
CGCCCAGGCAGGCCAATTTGAGCTCAACGCGATGATGCCGCTGATTGCCTATAACCTCCTGCATAGCTTGCAGATCCTCA
ACAACAGCCTGCGCGTCTTTACCGAGCGCTGCGTCCGCGGCATCACCGCCCAGGCGGATCGCTGCCGCAGCTATGCTGAG
GGGACGCTGGCTTTGGTGACTGCCCTCAATCCCTACATTGGCTACCTGCAGGCGGCAGAGGTGGCTAAGATCTCCCTGGA
AACCGGCAAGCCCATCCGCCAGATCGTGTTGGAGCGCAACTTGATGAGCCCGGAACAGGTGGCCAAGGCGTTGAATCTGG
AGGCGATGAGCCAGATGCGCTCTTAG

Upstream 100 bases:

>100_bases
GGCTGCTCTCCAGGAAAAGGGATCCCTACCTCTACAGGAACCTTCCCAAAAACCTGCCAGCTAGAGCTGTGCCATGGTTC
CAGAGACAGACCCACTGCAA

Downstream 100 bases:

>100_bases
CCCCTCCGCGAAAAGGGCAATTAGCCCTCTCGCCTGCCCCTTCCCCCTAGACTAAGTAAGACTCTCCTTTGCCAAGGCAG
GCCATGACCGGAGAACGACT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRSERDAMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELGCIPPDVAKAIVQAADEILQG
SLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKGRYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDLLGT
LDNLVAALEQKAQEFHGILKAGRTHLQDAVPIRLGDSFGAYAAIFAQHRRWIEEASQALGSLGLGGSAVGTGLNTHPQYR
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDAIKMANDLRLMDSGPKTGLKEIQLPPVQPGSSIMPG
KYNPVMAEMLNMVCFQVIGLDTAILLAAQAGQFELNAMMPLIAYNLLHSLQILNNSLRVFTERCVRGITAQADRCRSYAE
GTLALVTALNPYIGYLQAAEVAKISLETGKPIRQIVLERNLMSPEQVAKALNLEAMSQMRS

Sequences:

>Translated_461_residues
MRSERDAMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELGCIPPDVAKAIVQAADEILQG
SLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKGRYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDLLGT
LDNLVAALEQKAQEFHGILKAGRTHLQDAVPIRLGDSFGAYAAIFAQHRRWIEEASQALGSLGLGGSAVGTGLNTHPQYR
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDAIKMANDLRLMDSGPKTGLKEIQLPPVQPGSSIMPG
KYNPVMAEMLNMVCFQVIGLDTAILLAAQAGQFELNAMMPLIAYNLLHSLQILNNSLRVFTERCVRGITAQADRCRSYAE
GTLALVTALNPYIGYLQAAEVAKISLETGKPIRQIVLERNLMSPEQVAKALNLEAMSQMRS
>Mature_461_residues
MRSERDAMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELGCIPPDVAKAIVQAADEILQG
SLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKGRYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDLLGT
LDNLVAALEQKAQEFHGILKAGRTHLQDAVPIRLGDSFGAYAAIFAQHRRWIEEASQALGSLGLGGSAVGTGLNTHPQYR
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDAIKMANDLRLMDSGPKTGLKEIQLPPVQPGSSIMPG
KYNPVMAEMLNMVCFQVIGLDTAILLAAQAGQFELNAMMPLIAYNLLHSLQILNNSLRVFTERCVRGITAQADRCRSYAE
GTLALVTALNPYIGYLQAAEVAKISLETGKPIRQIVLERNLMSPEQVAKALNLEAMSQMRS

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=449, Percent_Identity=43.4298440979955, Blast_Score=356, Evalue=3e-98,
Organism=Escherichia coli, GI87082375, Length=459, Percent_Identity=45.7516339869281, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787896, Length=429, Percent_Identity=44.2890442890443, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17553882, Length=459, Percent_Identity=43.5729847494553, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565146, Length=322, Percent_Identity=41.6149068322981, Blast_Score=248, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6324993, Length=459, Percent_Identity=42.2657952069717, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640179, Length=460, Percent_Identity=41.9565217391304, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24640177, Length=460, Percent_Identity=41.9565217391304, Blast_Score=357, Evalue=7e-99,
Organism=Drosophila melanogaster, GI24662684, Length=436, Percent_Identity=41.2844036697248, Blast_Score=337, Evalue=8e-93,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=39.0455531453362, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24583245, Length=438, Percent_Identity=38.1278538812785, Blast_Score=328, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50231; Mature: 50231

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSERDAMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELG
CCCCHHHCCHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
CIPPDVAKAIVQAADEILQGSLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
RYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDLLGTLDNLVAALEQKAQEFHGILK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRTHLQDAVPIRLGDSFGAYAAIFAQHRRWIEEASQALGSLGLGGSAVGTGLNTHPQYR
HHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHH
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDAIKMANDLRLMDSGPK
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCC
TGLKEIQLPPVQPGSSIMPGKYNPVMAEMLNMVCFQVIGLDTAILLAAQAGQFELNAMMP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LIAYNLLHSLQILNNSLRVFTERCVRGITAQADRCRSYAEGTLALVTALNPYIGYLQAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VAKISLETGKPIRQIVLERNLMSPEQVAKALNLEAMSQMRS
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSERDAMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELG
CCCCHHHCCHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
CIPPDVAKAIVQAADEILQGSLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
RYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDLLGTLDNLVAALEQKAQEFHGILK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRTHLQDAVPIRLGDSFGAYAAIFAQHRRWIEEASQALGSLGLGGSAVGTGLNTHPQYR
HHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHH
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDAIKMANDLRLMDSGPK
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCC
TGLKEIQLPPVQPGSSIMPGKYNPVMAEMLNMVCFQVIGLDTAILLAAQAGQFELNAMMP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LIAYNLLHSLQILNNSLRVFTERCVRGITAQADRCRSYAEGTLALVTALNPYIGYLQAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VAKISLETGKPIRQIVLERNLMSPEQVAKALNLEAMSQMRS
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]