Click here to switch to the map view.

The map label for this gene is tyrS

Identifier: 86606736

GI number: 86606736

Start: 2111265

End: 2112518

Strand: Reverse

Name: tyrS

Synonym: CYA_2096

Alternate gene names: 86606736

Gene position: 2112518-2111265 (Counterclockwise)

Preceding gene: 86606737

Following gene: 86606733

Centisome position: 72.03

GC content: 61.88

Gene sequence:

>1254_bases
ATGGAGCCAACTTCGAGAACATTTCCAACTGCTGTCTCGTCAGCCCAGGGAGATCCCCGCATCGCCGAGATCACGCAGCG
GGGAGTGGCCGAGGTCTTCCCAGGGGGAGCGGCAGCCTTGGCGGAGCGCCTGGCCACCACCGATCGCCCCTTGCGGGTGA
AGCTGGGCATCGATCCCACGCGGCCAGATCTGCACCTGGGCCATGCAGTGGTCTTGCGCAAGTTGCGCCAATTCCAGGAT
GCTGGCCATGTGGCCATCCTCCTCATCGGGGATTTTACAGCCCTGGTCGGGGATCCCACCGGCCAATCGGAGGCCCGTCC
CCGCCTCACCCCGGCAGAAGTGGAGGAAAACGCCCGCACCTACTTGGAGCAAGCCGGCAAGATTTTGGATTTTGAGACCC
CCGGTCGGCTGGAGGTGCGCCGCAACAGCGAGTGGCTAGCCCGCCTCACCCTTAGCGAGCTCATTGAGCTCCAAGCCCAG
ATGACAGTGGGGCAGATGTTGGCCAAGGAAGACTTTGCCCAGCGCTACCGCTCGGGAACGCCCATCTACTTGCACGAATT
TCTCTACCCTCTCCTGCAAGGCTACGACTCGGTGGCGCTGGAGGCGGACGTGGAGTTGGGGGGAACTGACCAGCGCTTTA
ACCTCCTGGTTGGCCGGGAGCTGCAACTGTGGAAGGGCCAAACCCCTCAATTTTGCCTGACGGTGCCCCTGTTGGAGGGC
TTAGATGGCTACCAGAAAATGTCCAAGTCCAAAAACAACTACGTGGGCCTGACCGAGGATCCCCTCACCATGTACTCCAA
GCTAGAGAAGGTGCCGGATCAGCTGGTGGAGCGCTACTTTGAGCTGCTCACCTCGGTGCCCCCCTCGGAGCTGCCGGCGG
ATCCCCGCCAGCGCCAGATCCTCTTGGCCAAGACCCTCGTGGCCCAATTTCACTCTTCTGAAGCCGCAGAAGAGGCGCAG
CGCACCGCCCAAGCGCTGGTGTTACAAGGCAATCTCGTTGAAACGGGATCCATCCCCACTTTTGCCATCCAGTGCTTGTC
TTACCCGGTAAAACTGAGCTACATCCTGCGCGAATCCGGCCTCTGCAAGAGCGCTGCCGAGGCCCGTCGGCAAATCCAAG
GGGGCGCCGTGCGGCTGGACGGGCAAAAGGTGGAGGATCCCGACCTGGAGTTTGCCCAAGCCTCTGAGCTGGACGGGCGG
GTTTTGCAGGTGGGCAAGAAGGCCTTCTGCCGCTTGGTGGTCGGCACAAACTAG

Upstream 100 bases:

>100_bases
AAGGGGAGCTGCCCTCCTCAGTCTCTATCCCCCGTCGCCAGAGTACCCCGAAGGTTTTGATGGGCTTAAAATAGCCCATG
GTGCTCATCGAGCCAAGTGG

Downstream 100 bases:

>100_bases
TCAGCTTTGGTTTTCTCCCCTCACCCCCAACCCCTCTATGAGGGGAGCTCGATGGTTCCACAACTATTCAACAGGTGGGG
ATGGGCTTAAAGCCGAGCCC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MEPTSRTFPTAVSSAQGDPRIAEITQRGVAEVFPGGAAALAERLATTDRPLRVKLGIDPTRPDLHLGHAVVLRKLRQFQD
AGHVAILLIGDFTALVGDPTGQSEARPRLTPAEVEENARTYLEQAGKILDFETPGRLEVRRNSEWLARLTLSELIELQAQ
MTVGQMLAKEDFAQRYRSGTPIYLHEFLYPLLQGYDSVALEADVELGGTDQRFNLLVGRELQLWKGQTPQFCLTVPLLEG
LDGYQKMSKSKNNYVGLTEDPLTMYSKLEKVPDQLVERYFELLTSVPPSELPADPRQRQILLAKTLVAQFHSSEAAEEAQ
RTAQALVLQGNLVETGSIPTFAIQCLSYPVKLSYILRESGLCKSAAEARRQIQGGAVRLDGQKVEDPDLEFAQASELDGR
VLQVGKKAFCRLVVGTN

Sequences:

>Translated_417_residues
MEPTSRTFPTAVSSAQGDPRIAEITQRGVAEVFPGGAAALAERLATTDRPLRVKLGIDPTRPDLHLGHAVVLRKLRQFQD
AGHVAILLIGDFTALVGDPTGQSEARPRLTPAEVEENARTYLEQAGKILDFETPGRLEVRRNSEWLARLTLSELIELQAQ
MTVGQMLAKEDFAQRYRSGTPIYLHEFLYPLLQGYDSVALEADVELGGTDQRFNLLVGRELQLWKGQTPQFCLTVPLLEG
LDGYQKMSKSKNNYVGLTEDPLTMYSKLEKVPDQLVERYFELLTSVPPSELPADPRQRQILLAKTLVAQFHSSEAAEEAQ
RTAQALVLQGNLVETGSIPTFAIQCLSYPVKLSYILRESGLCKSAAEARRQIQGGAVRLDGQKVEDPDLEFAQASELDGR
VLQVGKKAFCRLVVGTN
>Mature_417_residues
MEPTSRTFPTAVSSAQGDPRIAEITQRGVAEVFPGGAAALAERLATTDRPLRVKLGIDPTRPDLHLGHAVVLRKLRQFQD
AGHVAILLIGDFTALVGDPTGQSEARPRLTPAEVEENARTYLEQAGKILDFETPGRLEVRRNSEWLARLTLSELIELQAQ
MTVGQMLAKEDFAQRYRSGTPIYLHEFLYPLLQGYDSVALEADVELGGTDQRFNLLVGRELQLWKGQTPQFCLTVPLLEG
LDGYQKMSKSKNNYVGLTEDPLTMYSKLEKVPDQLVERYFELLTSVPPSELPADPRQRQILLAKTLVAQFHSSEAAEEAQ
RTAQALVLQGNLVETGSIPTFAIQCLSYPVKLSYILRESGLCKSAAEARRQIQGGAVRLDGQKVEDPDLEFAQASELDGR
VLQVGKKAFCRLVVGTN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=403, Percent_Identity=29.2803970223325, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787925, Length=423, Percent_Identity=31.2056737588652, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25145802, Length=299, Percent_Identity=28.0936454849498, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325160, Length=464, Percent_Identity=25.8620689655172, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130383, Length=434, Percent_Identity=26.4976958525346, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_SYNJA (Q2JSX8)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_475499.1
- ProteinModelPortal:   Q2JSX8
- SMR:   Q2JSX8
- STRING:   Q2JSX8
- GeneID:   3899240
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_2096
- TIGR:   CYA_2096
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   FKEGIPI
- ProtClustDB:   PRK05912
- BioCyc:   SSP321327:CYA_2096-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46068; Mature: 46068

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 249-249

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPTSRTFPTAVSSAQGDPRIAEITQRGVAEVFPGGAAALAERLATTDRPLRVKLGIDPT
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCC
RPDLHLGHAVVLRKLRQFQDAGHVAILLIGDFTALVGDPTGQSEARPRLTPAEVEENART
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
YLEQAGKILDFETPGRLEVRRNSEWLARLTLSELIELQAQMTVGQMLAKEDFAQRYRSGT
HHHHCCCEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIYLHEFLYPLLQGYDSVALEADVELGGTDQRFNLLVGRELQLWKGQTPQFCLTVPLLEG
CEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCEEHCCCCCCCCEEEECHHHHC
LDGYQKMSKSKNNYVGLTEDPLTMYSKLEKVPDQLVERYFELLTSVPPSELPADPRQRQI
CHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LLAKTLVAQFHSSEAAEEAQRTAQALVLQGNLVETGSIPTFAIQCLSYPVKLSYILRESG
HHHHHHHHHHHCHHHHHHHHHHHHHHHEECCEEECCCCHHHHHHHHCCCHHHHHHHHHCC
LCKSAAEARRQIQGGAVRLDGQKVEDPDLEFAQASELDGRVLQVGKKAFCRLVVGTN
CCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHCCCCHHHHHHHHHHEEEEECCC
>Mature Secondary Structure
MEPTSRTFPTAVSSAQGDPRIAEITQRGVAEVFPGGAAALAERLATTDRPLRVKLGIDPT
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCC
RPDLHLGHAVVLRKLRQFQDAGHVAILLIGDFTALVGDPTGQSEARPRLTPAEVEENART
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
YLEQAGKILDFETPGRLEVRRNSEWLARLTLSELIELQAQMTVGQMLAKEDFAQRYRSGT
HHHHCCCEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIYLHEFLYPLLQGYDSVALEADVELGGTDQRFNLLVGRELQLWKGQTPQFCLTVPLLEG
CEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCEEHCCCCCCCCEEEECHHHHC
LDGYQKMSKSKNNYVGLTEDPLTMYSKLEKVPDQLVERYFELLTSVPPSELPADPRQRQI
CHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LLAKTLVAQFHSSEAAEEAQRTAQALVLQGNLVETGSIPTFAIQCLSYPVKLSYILRESG
HHHHHHHHHHHCHHHHHHHHHHHHHHHEECCEEECCCCHHHHHHHHCCCHHHHHHHHHCC
LCKSAAEARRQIQGGAVRLDGQKVEDPDLEFAQASELDGRVLQVGKKAFCRLVVGTN
CCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHCCCCHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA