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The map label for this gene is glgB

Identifier: 86606702

GI number: 86606702

Start: 2081587

End: 2083899

Strand: Reverse

Name: glgB

Synonym: CYA_2062

Alternate gene names: 86606702

Gene position: 2083899-2081587 (Counterclockwise)

Preceding gene: 86606703

Following gene: 86606701

Centisome position: 71.06

GC content: 57.8

Gene sequence:

>2313_bases
ATGGGCTTTCTCCTGTCTGCCGAACAAGTGCTTCAGTTCGTCACCAACCAGTGGCAGGATCCCTATGCTGTTTTGGGGCC
GCAGCAGATAGAGCAGGGGGAGACGTCCTTCTGGCTGGTGCGGGCCCTGGTGCCCAATGCCAAACAGGTTTGGTTGGTAG
AACGCGCCACTGGGCAGGCCTACCCCATGCAGCCGCTGCATCCAGAAACGTTGTTTGAGCTGTGCTTTGCGCCTGGCACG
CCGGTTCCAGACTACTTTCTGCGGGCGCAGCGGGTCTGGGATCCGGAGGGCCAGCACTTGGAAGAGTGGGAGGATCCCTA
TCGCTTTCCCCTGGAGAAGGTCAACCACATCGGCGAGCTGGATCGCTACCTCTTCAACGAGGGCAACCACCACCGCATCT
ACGAGAAGTTGGGCGCTCATCCCATCAGCGTAGATGGGGTTCAGGGAGTCCACTTTGCCGTCTGGGCTCCCAACGCCCGC
AATGTCAGTGTCATTGGCGACTTCAACCACTGGGATGGCCGCCAACACCAGATGAAGCGGCTGGGCGAAAGCGGCATCTG
GGCGGTGTTCATCCCTGGTGTTGGGCCAGGAGCCGTCTACAAGTACGAGGTGAAAACTGCCTGGGGGGATATCTACGAAA
AGTCAGATCCCTATGGCTTTCAGCAGGAGGTGCGACCCAAAACCGGCTCAATTGTAGCCGACCTCCACACCTATACCTGG
CACGACCAGGAGTGGCTGGAAAAGCGGGCTGCCACCGACCCCCTGCGCTCGCCCATCTCGGTGTACGAGGTGCATTTGGG
ATCCTGGATGCACGCTTCCACGGAAGACCCCCCCGCTGATGGGCACCTGGTGCCGGTGGAGCAAAAGCCCAACACCCGCT
TTCTTACCTACCGGGAGCTGGCGGACAAATTGATCCCCTATGTCAAGGAGCTGGGCTTTACCCACATCGAGCTCTTACCC
GTGGCCGAGCACCCCTTCGACGGCTCCTGGGGCTACCAGGTTATTGGCTACTACGCGGTAACCTCCCGCTACGGCTCGCC
CCAAGACTTCATGTACTTTGTCGATCGCGCCCACCAGGAAGGGATCGGCGTCATTGTCGATTGGGTGCCGGGGCATTTCC
CCAAAGATGGGCATGGGCTGGCCTTTTTTGACGGTACCCACCTCTACGAATATGCCGACCCCCGCAAGGGCGAGCACAAG
GGCTGGGGCACTTTGGTTTTCAACTACGGCCGCAACGAGGTGCGCAACTATCTAATAGCCAATGCCCTCTTTTGGTTTGA
CAAATACCACATCGACGGCCTTCGGGTGGATGCGGTGGCCTCAATGCTCTATCTGGACTACGACCGCAAAGAATGGATCC
CCAACTGCTACGGCGGGCGAGAACACCTGGAGGCCATCGACTTTTTTCGCCAGCTCAACACCCTTATTTTCAAGTACTAC
CCTGGCGTTCTTTCCATTGCCGAGGAATCCACTGCCTGGCCAATGGTAACCTGGCCAACCCATGTGGGTGGTCTGGGCTT
TAACCTCAAGTGGAACATGGGCTGGATGCACGACATGCTGAATTATTTTCGCATGGATCCCTGGTTCCGCCAGTTCCATC
ACAACCTAGTGACTTTCAGCCTCATGTATGCCTTCAGTGAAAATTACATGCTGGCTTTTTCCCACGATGAGGTAGTGCAC
GGCAAAAGCCACATGCTGGGCAAAATGCCAGGAGATCTGTGGCACAAGTTCGCCAGCTTGCGGGCTCTCTACGGCTACAT
GTTCACTCACCCTGGCAAGAAAACCCTGTTCATGAGCATGGAGTTTGGCCAGTGGAACGAGTGGAATGTTTGGGCAGATC
TAGACTGGGAGCTGCTGCAGTATGAGCCCCACGCCAAGCTGCGCCACTACGTAGCCACTCTCAACCAGCTGCTGCGCTCG
CAACCGGCTCTCTACACCCAAGACACCAAGCCAGAAGGCTTCCGCTGGATTGACTGCAGCGACCATCGGGGCATTATCTC
CTTTATCCGATATGGGGAGGATCCCAGGGAGTGGCTAGTGGTAGTCTGTAACTTTACGCCGGTGGTTTGGCCGAATTACC
GCATTGGCGTGCCGCAGCGAGGGTTTTACCGGGAACTGCTCAACAGCGACGCGGTAGAGTTCTGGGGCAGCGGGGTTGGC
AACCTAGGGGGCAAGTGGACGGATGACTGGCCCTACCACAACCTGCCCTACTCGCTTGAGCTCTGCCTGCCCCCTCTTTC
AACCTTGGTGCTGAAGTGGCAGCCGCCCCAGCTGGCGGAGGATTCAGGCGAAAACAAAGCTATGCTGGAGTGA

Upstream 100 bases:

>100_bases
TACAGCCCGTCAGGGCTTAGCGGGAGAGGATGTCACGAGCTGCCAAAAGCGGCAGCCGACGACCGCTAGACTGAGATTGT
TGCTTTGTTGAAGCTTGCCG

Downstream 100 bases:

>100_bases
GTACGGCCTTCCCGCCTCATTGCCATGCGAGATCTGCTGCGCGACATCCTTCGAGACTATTCTGGCTTTGTGGAACTGCG
CTACCACAGCAAAGTCTTTC

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MGFLLSAEQVLQFVTNQWQDPYAVLGPQQIEQGETSFWLVRALVPNAKQVWLVERATGQAYPMQPLHPETLFELCFAPGT
PVPDYFLRAQRVWDPEGQHLEEWEDPYRFPLEKVNHIGELDRYLFNEGNHHRIYEKLGAHPISVDGVQGVHFAVWAPNAR
NVSVIGDFNHWDGRQHQMKRLGESGIWAVFIPGVGPGAVYKYEVKTAWGDIYEKSDPYGFQQEVRPKTGSIVADLHTYTW
HDQEWLEKRAATDPLRSPISVYEVHLGSWMHASTEDPPADGHLVPVEQKPNTRFLTYRELADKLIPYVKELGFTHIELLP
VAEHPFDGSWGYQVIGYYAVTSRYGSPQDFMYFVDRAHQEGIGVIVDWVPGHFPKDGHGLAFFDGTHLYEYADPRKGEHK
GWGTLVFNYGRNEVRNYLIANALFWFDKYHIDGLRVDAVASMLYLDYDRKEWIPNCYGGREHLEAIDFFRQLNTLIFKYY
PGVLSIAEESTAWPMVTWPTHVGGLGFNLKWNMGWMHDMLNYFRMDPWFRQFHHNLVTFSLMYAFSENYMLAFSHDEVVH
GKSHMLGKMPGDLWHKFASLRALYGYMFTHPGKKTLFMSMEFGQWNEWNVWADLDWELLQYEPHAKLRHYVATLNQLLRS
QPALYTQDTKPEGFRWIDCSDHRGIISFIRYGEDPREWLVVVCNFTPVVWPNYRIGVPQRGFYRELLNSDAVEFWGSGVG
NLGGKWTDDWPYHNLPYSLELCLPPLSTLVLKWQPPQLAEDSGENKAMLE

Sequences:

>Translated_770_residues
MGFLLSAEQVLQFVTNQWQDPYAVLGPQQIEQGETSFWLVRALVPNAKQVWLVERATGQAYPMQPLHPETLFELCFAPGT
PVPDYFLRAQRVWDPEGQHLEEWEDPYRFPLEKVNHIGELDRYLFNEGNHHRIYEKLGAHPISVDGVQGVHFAVWAPNAR
NVSVIGDFNHWDGRQHQMKRLGESGIWAVFIPGVGPGAVYKYEVKTAWGDIYEKSDPYGFQQEVRPKTGSIVADLHTYTW
HDQEWLEKRAATDPLRSPISVYEVHLGSWMHASTEDPPADGHLVPVEQKPNTRFLTYRELADKLIPYVKELGFTHIELLP
VAEHPFDGSWGYQVIGYYAVTSRYGSPQDFMYFVDRAHQEGIGVIVDWVPGHFPKDGHGLAFFDGTHLYEYADPRKGEHK
GWGTLVFNYGRNEVRNYLIANALFWFDKYHIDGLRVDAVASMLYLDYDRKEWIPNCYGGREHLEAIDFFRQLNTLIFKYY
PGVLSIAEESTAWPMVTWPTHVGGLGFNLKWNMGWMHDMLNYFRMDPWFRQFHHNLVTFSLMYAFSENYMLAFSHDEVVH
GKSHMLGKMPGDLWHKFASLRALYGYMFTHPGKKTLFMSMEFGQWNEWNVWADLDWELLQYEPHAKLRHYVATLNQLLRS
QPALYTQDTKPEGFRWIDCSDHRGIISFIRYGEDPREWLVVVCNFTPVVWPNYRIGVPQRGFYRELLNSDAVEFWGSGVG
NLGGKWTDDWPYHNLPYSLELCLPPLSTLVLKWQPPQLAEDSGENKAMLE
>Mature_769_residues
GFLLSAEQVLQFVTNQWQDPYAVLGPQQIEQGETSFWLVRALVPNAKQVWLVERATGQAYPMQPLHPETLFELCFAPGTP
VPDYFLRAQRVWDPEGQHLEEWEDPYRFPLEKVNHIGELDRYLFNEGNHHRIYEKLGAHPISVDGVQGVHFAVWAPNARN
VSVIGDFNHWDGRQHQMKRLGESGIWAVFIPGVGPGAVYKYEVKTAWGDIYEKSDPYGFQQEVRPKTGSIVADLHTYTWH
DQEWLEKRAATDPLRSPISVYEVHLGSWMHASTEDPPADGHLVPVEQKPNTRFLTYRELADKLIPYVKELGFTHIELLPV
AEHPFDGSWGYQVIGYYAVTSRYGSPQDFMYFVDRAHQEGIGVIVDWVPGHFPKDGHGLAFFDGTHLYEYADPRKGEHKG
WGTLVFNYGRNEVRNYLIANALFWFDKYHIDGLRVDAVASMLYLDYDRKEWIPNCYGGREHLEAIDFFRQLNTLIFKYYP
GVLSIAEESTAWPMVTWPTHVGGLGFNLKWNMGWMHDMLNYFRMDPWFRQFHHNLVTFSLMYAFSENYMLAFSHDEVVHG
KSHMLGKMPGDLWHKFASLRALYGYMFTHPGKKTLFMSMEFGQWNEWNVWADLDWELLQYEPHAKLRHYVATLNQLLRSQ
PALYTQDTKPEGFRWIDCSDHRGIISFIRYGEDPREWLVVVCNFTPVVWPNYRIGVPQRGFYRELLNSDAVEFWGSGVGN
LGGKWTDDWPYHNLPYSLELCLPPLSTLVLKWQPPQLAEDSGENKAMLE

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=667, Percent_Identity=26.6866566716642, Blast_Score=176, Evalue=1e-43,
Organism=Escherichia coli, GI1789839, Length=758, Percent_Identity=47.0976253298153, Blast_Score=668, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=393, Percent_Identity=30.0254452926209, Blast_Score=174, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI32564391, Length=358, Percent_Identity=29.3296089385475, Blast_Score=151, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320826, Length=399, Percent_Identity=27.5689223057644, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28573410, Length=614, Percent_Identity=27.6872964169381, Blast_Score=180, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_SYNJA (Q2JT08)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_475465.1
- ProteinModelPortal:   Q2JT08
- STRING:   Q2JT08
- GeneID:   3899245
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_2062
- TIGR:   CYA_2062
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- ProtClustDB:   PRK05402
- BioCyc:   SSP321327:CYA_2062-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 89562; Mature: 89431

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: ACT_SITE 332-332 ACT_SITE 367-367 ACT_SITE 372-372 ACT_SITE 435-435 ACT_SITE 437-437 ACT_SITE 488-488 ACT_SITE 555-555 ACT_SITE 556-556

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLLSAEQVLQFVTNQWQDPYAVLGPQQIEQGETSFWLVRALVPNAKQVWLVERATGQA
CCCCCCHHHHHHHHHHCCCCCEEECCCHHHHCCCCCEEEEEHHCCCCCEEEEEEECCCCC
YPMQPLHPETLFELCFAPGTPVPDYFLRAQRVWDPEGQHLEEWEDPYRFPLEKVNHIGEL
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
DRYLFNEGNHHRIYEKLGAHPISVDGVQGVHFAVWAPNARNVSVIGDFNHWDGRQHQMKR
HHHHHCCCCCCHHHHHHCCCCCEECCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHH
LGESGIWAVFIPGVGPGAVYKYEVKTAWGDIYEKSDPYGFQQEVRPKTGSIVADLHTYTW
CCCCCEEEEEECCCCCCCEEEEEEEHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEEEEC
HDQEWLEKRAATDPLRSPISVYEVHLGSWMHASTEDPPADGHLVPVEQKPNTRFLTYREL
CCHHHHHHHHCCCHHCCCCEEEEEECCCEEECCCCCCCCCCCEEECCCCCCCEEEEHHHH
ADKLIPYVKELGFTHIELLPVAEHPFDGSWGYQVIGYYAVTSRYGSPQDFMYFVDRAHQE
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCC
GIGVIVDWVPGHFPKDGHGLAFFDGTHLYEYADPRKGEHKGWGTLVFNYGRNEVRNYLIA
CCEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHH
NALFWFDKYHIDGLRVDAVASMLYLDYDRKEWIPNCYGGREHLEAIDFFRQLNTLIFKYY
HHHHHHHHHCCCCEEHHHHHHHHHEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
PGVLSIAEESTAWPMVTWPTHVGGLGFNLKWNMGWMHDMLNYFRMDPWFRQFHHNLVTFS
CCCEEECCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHH
LMYAFSENYMLAFSHDEVVHGKSHMLGKMPGDLWHKFASLRALYGYMFTHPGKKTLFMSM
HHHHHCCCEEEEECCCCEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
EFGQWNEWNVWADLDWELLQYEPHAKLRHYVATLNQLLRSQPALYTQDTKPEGFRWIDCS
ECCCCCCCEEEECCCHHEEECCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEECC
DHRGIISFIRYGEDPREWLVVVCNFTPVVWPNYRIGVPQRGFYRELLNSDAVEFWGSGVG
CCCCHHHHHHCCCCCHHEEEEEECCCCEECCCEEECCCCCHHHHHHHCCCHHHHHCCCCC
NLGGKWTDDWPYHNLPYSLELCLPPLSTLVLKWQPPQLAEDSGENKAMLE
CCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GFLLSAEQVLQFVTNQWQDPYAVLGPQQIEQGETSFWLVRALVPNAKQVWLVERATGQA
CCCCCHHHHHHHHHHCCCCCEEECCCHHHHCCCCCEEEEEHHCCCCCEEEEEEECCCCC
YPMQPLHPETLFELCFAPGTPVPDYFLRAQRVWDPEGQHLEEWEDPYRFPLEKVNHIGEL
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
DRYLFNEGNHHRIYEKLGAHPISVDGVQGVHFAVWAPNARNVSVIGDFNHWDGRQHQMKR
HHHHHCCCCCCHHHHHHCCCCCEECCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHH
LGESGIWAVFIPGVGPGAVYKYEVKTAWGDIYEKSDPYGFQQEVRPKTGSIVADLHTYTW
CCCCCEEEEEECCCCCCCEEEEEEEHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEEEEC
HDQEWLEKRAATDPLRSPISVYEVHLGSWMHASTEDPPADGHLVPVEQKPNTRFLTYREL
CCHHHHHHHHCCCHHCCCCEEEEEECCCEEECCCCCCCCCCCEEECCCCCCCEEEEHHHH
ADKLIPYVKELGFTHIELLPVAEHPFDGSWGYQVIGYYAVTSRYGSPQDFMYFVDRAHQE
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCC
GIGVIVDWVPGHFPKDGHGLAFFDGTHLYEYADPRKGEHKGWGTLVFNYGRNEVRNYLIA
CCEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHH
NALFWFDKYHIDGLRVDAVASMLYLDYDRKEWIPNCYGGREHLEAIDFFRQLNTLIFKYY
HHHHHHHHHCCCCEEHHHHHHHHHEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
PGVLSIAEESTAWPMVTWPTHVGGLGFNLKWNMGWMHDMLNYFRMDPWFRQFHHNLVTFS
CCCEEECCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHH
LMYAFSENYMLAFSHDEVVHGKSHMLGKMPGDLWHKFASLRALYGYMFTHPGKKTLFMSM
HHHHHCCCEEEEECCCCEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
EFGQWNEWNVWADLDWELLQYEPHAKLRHYVATLNQLLRSQPALYTQDTKPEGFRWIDCS
ECCCCCCCEEEECCCHHEEECCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEECC
DHRGIISFIRYGEDPREWLVVVCNFTPVVWPNYRIGVPQRGFYRELLNSDAVEFWGSGVG
CCCCHHHHHHCCCCCHHEEEEEECCCCEECCCEEECCCCCHHHHHHHCCCHHHHHCCCCC
NLGGKWTDDWPYHNLPYSLELCLPPLSTLVLKWQPPQLAEDSGENKAMLE
CCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA