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The map label for this gene is recF

Identifier: 86606664

GI number: 86606664

Start: 2039458

End: 2040600

Strand: Reverse

Name: recF

Synonym: CYA_2022

Alternate gene names: 86606664

Gene position: 2040600-2039458 (Counterclockwise)

Preceding gene: 86606665

Following gene: 86606662

Centisome position: 69.58

GC content: 65.62

Gene sequence:

>1143_bases
GTGTACCTGCGTTTTCTGCACCTGTGGCACTTTCGCAACTACCGGGATCAAAAGATCTCCTTCGAGGCGCCCAAGACCAT
CCTGGTGGGGGAGAACGCCCAGGGCAAGACCAACCTCTTGGAGGCGGTGGAGCTCTTGGCCACCCTGCGCTCGCGGCGGG
CCGGTCGGGATCGGGAGTTGGTGCAGCAGGGGGCAGAGAAGGCCCGGATCGCCGCCACGGTGGAGCGGCTGGGGGTGGCC
CACGAGCTGGCGATGGAGCTGCGCAGCCAAGGGGGGCGATCCCTAAGGGTTAACGGCCAGGGGCTACGGCGACAATCGGA
CTTTTTGGGGCAGGTGAGTGCCGTGGTGTTCTCCAGCCTGGATTTGGAGCTGGTGCGGGGAGCTCCAGAAGCGCGGCGCA
CCTGGCTGGACGGGGTGTTGCTTCAACTGGAGCCGGCTTACCTGGGCCTGGTGGAGCAGTATCGTCAGATCCTCAAGCAG
CGCAATGCCCTCTTGAAGCAGGATCCCTTGGCTGCGGGCGACAAAGTACCTCAGATGGCCTTTTGGGACGCGCAACTGGC
CACCCTTGGCAGCCGCATCCTGCGCCGCCGCGCCCGGCTGTTGCAGCGGCTAGAACCCCTGGCCGCCCGCTGGCACCAGG
CCATCAGCGGCGGACGGGAGACCCTCAGCCTCACCTACCGCCCGCAGGTGCCCCTGCCGGATCCCCAGGCCGATCCCAAG
GTGGTTCAAGCCCAATTTTTGGCCGCGATCCGCGCCAAGGCTGCTGCCGAACAGGCCCTGGGCACCTCTCTGGTTGGCCC
CCACCGAGACGAGGTGGAGCTGGGCATCGACGGAGTTGCCGCCCGCGCCTATGGATCCCAGGGGCAGCAGCGCACCTTGG
TCTTGGCCCTCAAATTGGCGGAGCTGGAACTGATCGAGCAGGTGAAGGGGGATCCGCCCCTGCTGCTGCTGGACGACGTG
CTGGCAGAGCTGGATCTGCACCGCCAAAACCAGCTCTTGGAGGCGATTCAAGAACGGGTGCAGACTTTGGTTACCACCAC
CCACCTGGGATCCTTCGACGCCGCTTGGCTCCAAGGGGCGCAAATCCTGCAAGTGCACCAGGGGCAGATTGCTCCCTCCC
CCGCCTCGCTCCCCTTGGCTTAA

Upstream 100 bases:

>100_bases
GGTCTGCTGACCCCGAGGCAGTTGCGTTAGAGTAAAAGCCCCCGCCGCGGGGGCTGTTCTTCTGCCCAGAAATCCAGAGC
CAAGAGCGCACGGCTGTCGA

Downstream 100 bases:

>100_bases
AGCAGGCCAATCCCATACCATACTCCCCTCTCCCTCTGGGAGAGGGGCCGGGGGTGAGGGCTCAGGGCCGCCCGCGATCC
AGGCTGCGATACTGGATGGC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MYLRFLHLWHFRNYRDQKISFEAPKTILVGENAQGKTNLLEAVELLATLRSRRAGRDRELVQQGAEKARIAATVERLGVA
HELAMELRSQGGRSLRVNGQGLRRQSDFLGQVSAVVFSSLDLELVRGAPEARRTWLDGVLLQLEPAYLGLVEQYRQILKQ
RNALLKQDPLAAGDKVPQMAFWDAQLATLGSRILRRRARLLQRLEPLAARWHQAISGGRETLSLTYRPQVPLPDPQADPK
VVQAQFLAAIRAKAAAEQALGTSLVGPHRDEVELGIDGVAARAYGSQGQQRTLVLALKLAELELIEQVKGDPPLLLLDDV
LAELDLHRQNQLLEAIQERVQTLVTTTHLGSFDAAWLQGAQILQVHQGQIAPSPASLPLA

Sequences:

>Translated_380_residues
MYLRFLHLWHFRNYRDQKISFEAPKTILVGENAQGKTNLLEAVELLATLRSRRAGRDRELVQQGAEKARIAATVERLGVA
HELAMELRSQGGRSLRVNGQGLRRQSDFLGQVSAVVFSSLDLELVRGAPEARRTWLDGVLLQLEPAYLGLVEQYRQILKQ
RNALLKQDPLAAGDKVPQMAFWDAQLATLGSRILRRRARLLQRLEPLAARWHQAISGGRETLSLTYRPQVPLPDPQADPK
VVQAQFLAAIRAKAAAEQALGTSLVGPHRDEVELGIDGVAARAYGSQGQQRTLVLALKLAELELIEQVKGDPPLLLLDDV
LAELDLHRQNQLLEAIQERVQTLVTTTHLGSFDAAWLQGAQILQVHQGQIAPSPASLPLA
>Mature_380_residues
MYLRFLHLWHFRNYRDQKISFEAPKTILVGENAQGKTNLLEAVELLATLRSRRAGRDRELVQQGAEKARIAATVERLGVA
HELAMELRSQGGRSLRVNGQGLRRQSDFLGQVSAVVFSSLDLELVRGAPEARRTWLDGVLLQLEPAYLGLVEQYRQILKQ
RNALLKQDPLAAGDKVPQMAFWDAQLATLGSRILRRRARLLQRLEPLAARWHQAISGGRETLSLTYRPQVPLPDPQADPK
VVQAQFLAAIRAKAAAEQALGTSLVGPHRDEVELGIDGVAARAYGSQGQQRTLVLALKLAELELIEQVKGDPPLLLLDDV
LAELDLHRQNQLLEAIQERVQTLVTTTHLGSFDAAWLQGAQILQVHQGQIAPSPASLPLA

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=377, Percent_Identity=24.9336870026525, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_SYNJA (Q2JQG8)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_475427.1
- ProteinModelPortal:   Q2JQG8
- SMR:   Q2JQG8
- STRING:   Q2JQG8
- GeneID:   3899390
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_2022
- TIGR:   CYA_2022
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   SSP321327:CYA_2022-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42117; Mature: 42117

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLRFLHLWHFRNYRDQKISFEAPKTILVGENAQGKTNLLEAVELLATLRSRRAGRDREL
CEEHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
VQQGAEKARIAATVERLGVAHELAMELRSQGGRSLRVNGQGLRRQSDFLGQVSAVVFSSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHC
DLELVRGAPEARRTWLDGVLLQLEPAYLGLVEQYRQILKQRNALLKQDPLAAGDKVPQMA
CHHHHHCCCHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
FWDAQLATLGSRILRRRARLLQRLEPLAARWHQAISGGRETLSLTYRPQVPLPDPQADPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHH
VVQAQFLAAIRAKAAAEQALGTSLVGPHRDEVELGIDGVAARAYGSQGQQRTLVLALKLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHCCCCCCEEEHHHHHHH
ELELIEQVKGDPPLLLLDDVLAELDLHRQNQLLEAIQERVQTLVTTTHLGSFDAAWLQGA
HHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCH
QILQVHQGQIAPSPASLPLA
HHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MYLRFLHLWHFRNYRDQKISFEAPKTILVGENAQGKTNLLEAVELLATLRSRRAGRDREL
CEEHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
VQQGAEKARIAATVERLGVAHELAMELRSQGGRSLRVNGQGLRRQSDFLGQVSAVVFSSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHC
DLELVRGAPEARRTWLDGVLLQLEPAYLGLVEQYRQILKQRNALLKQDPLAAGDKVPQMA
CHHHHHCCCHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
FWDAQLATLGSRILRRRARLLQRLEPLAARWHQAISGGRETLSLTYRPQVPLPDPQADPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHH
VVQAQFLAAIRAKAAAEQALGTSLVGPHRDEVELGIDGVAARAYGSQGQQRTLVLALKLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHCCCCCCEEEHHHHHHH
ELELIEQVKGDPPLLLLDDVLAELDLHRQNQLLEAIQERVQTLVTTTHLGSFDAAWLQGA
HHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCH
QILQVHQGQIAPSPASLPLA
HHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA