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The map label for this gene is recG

Identifier: 86606655

GI number: 86606655

Start: 2028491

End: 2030812

Strand: Reverse

Name: recG

Synonym: CYA_2013

Alternate gene names: 86606655

Gene position: 2030812-2028491 (Counterclockwise)

Preceding gene: 86606656

Following gene: 86606652

Centisome position: 69.25

GC content: 64.73

Gene sequence:

>2322_bases
GTGTTTGGCCAGGATCCCCCTGCGGGTTGGACGCTGGATGGAGAGGAGCGACGGCGGTGGAAAGAACTGGGATCCCGCTA
TCGCCTCTACCCCCAGTTGGAGCTGGCGGAGCGGCAGCACCTGGTGGCGGAAACCCGCCGCTGGCTGGATCGGCTGCGTC
GTCAGCAAGAGCAAGCCCAATCTGGCCCACCCAGCCCTGCTTTGCCCCCGCCCAAGGGCCTCAGCTTGGAGCAGCCGCTG
CAATTTCTCAAGGGCATTGGCCCCAAGTCGGCGGAGAAGCTGGCACAACTGGGCCTGCACACTGTGCGCGATGCCCTCTA
CTACTTCCCACGGGATCACCTGGACTATTCCCGCCGGGTGCTCATCCGCCAGGCCAAGGTGGGGGAGACGGTCACCCTGG
TGGGGACGGTGAAAAGCTGCAGTTGCTTTACCAGCCCCAAAAACCCCTTGCTGACCATCTTGAACCTCAAGATTGCCGAC
AAAAGTGGCATAGTCACCCTCAGCCGCTTCTACAGCGGGCGGCAATTTGCCCAGCGCAGTTGGCAGGCGGCCCTGGAGCG
GCAGTACCCCAAGGGGGCAGTGGTGGCGGCCAGCGGCCTGGTGAAAAAGGGCCGCTTGGGCTTGACGCTGGACAACCCGC
AACTGGAGGTGCTGGGGGGAGAGGGCGAGGATCCGGAGAGATTGGGCAAGATTCTACCCGTTTACCCCCTTTCAGAAGGG
ATCCCCGCCGATCTGGTGCGCAAGGCCGTCCAGCTCGCCCTGCCGGCAGCTGCCTTGGTGGAGGATCCCTTGCCGGAGCC
CCTGAAGCGGCAGTTGCAGCTGTTGGACTTGCCCCTTGCCCTGCAGCAGATCCACTTTCCCAAGACGCAAGAGCTGCTGG
CCCAGGCCCGGCGGCGGCTGGTGTTCGATGAGTTCTTCTACCTGCAACTTAGCCTCTTGCAGCGGCGGCAGCGCTACCGC
GCCCAAGCTCAGGCTGTACCTCGCTACCAGCCGGCCAGAGGGGAGCTCTTGGATCGCTTCTACCAGATCCTGCCCTTCCA
GCTTACTGCGGGCCAGCAGCGGGCCATCGATGAGATCCTGGCGGATCTGCGGGATCCCTTGCCCATGAACCGCCTGCTGC
AGGGGGATGTGGGATCCGGCAAAACTGTGGTGGCGGTGGCGGCCTTGCTGGCGGCGATTCAATCCGGCTGGCAGGCGGCC
CTCATGGCCCCCACCGAGGTCTTGGCCGAGCAGCACTACCGCAAGCTGGTGGAGTGGCTGAGCCAACTGCAAGTGCCCGT
TGAACTCTTGACCGGCTCCACCCCCCCCGCCAAGCGGCGCGAGATCCTGCGGCAGTTGCAGACCGGGGAGCTGCCCCTAG
TTGTTGGTACCCATGCCCTCATCCAGCCGGCGGTGCAGTTTCGCAACCTGGGCTTGGTGGTTATCGACGAGCAGCACCGC
TTTGGCGTCGAACAGCGGGCTGCCCTGCAACAGAAAGGGGATCACCCCGATGTCCTGACCATGACGGCCACCCCCATCCC
CCGCACCCTTACCCTGGCCCTGCACGGGGATCTGGATGTTACCCAAATTGACGAGCTGCCCCCGGGGCGCAAGCCGGTGC
ATACGGTGGTAGCTCGACCCGGGGATCGCCTGCAGGTGGTGCGGCTGATGGAGCGGGAAATTGCCCAGGGACGGCAGGCC
TACGTGGTGCTGCCCCTGATTGAGGAGTCGGAGAAGCTGGATCTCAAGTCAGCCATTGAAGAGCACCAGCGCCTGCAGGA
GAAGGTCTTCCCCCAGTTCCGGGTGGGCCTGCTGCACGGGCGCCTAACTAGCAGCGAGAAAGAGGCTGTCATCGAAGCCT
TTCGCCGGCGGGAGCTGGATATCCTCGTCTCCACCACAGTGGTGGAAGTAGGGGTGGATGTGCCCAACGCCTCGGTGATG
CTGGTGGAGCATGCCGAGCGCTTTGGCCTCTCCCAGCTCCACCAACTGCGGGGGCGGGTGGGGCGGGGATCCGACCAGGC
CTACTGCATTCTAATGACGGGATCCCAAAGCGAAGAGGCCCTGCGTCGGCTAAAAGTCCTGGAGCAGTCCCACGATGGCT
TTTTCATTGCCGAGATGGACCTGCGCTTCCGCGGCCCTGGGGAGGTGATGGGCACCCGCCAGTCGGGGCTGCCCGACTTT
GCCCTGGCCAGCCTCATGGACGACCAAGAGGTGCTGCAGTTGGCCCGCCAGGCGGCCGAGCAGCTCATTGCCCAAGACCC
GGAGCTGCAGCAGTGGCCCCGCCTGCGCCGCATACTGGAAGAACGCCAGGCCCGCCTGTTGGGGCCGGCTTTGTTGACGT
GA

Upstream 100 bases:

>100_bases
GGATCTACAGCGGCTTCAGCGCGCCCTTTCTCTAGAGGCGGAGTTGGGATTTACCGACCTGCGGGGGCGAGAGCAGACCT
TCTCCCAGTTTTTGCAGAGC

Downstream 100 bases:

>100_bases
CATACTCCCGTCCCTCACCCTAAAGCGCTCCGCGCCGCTGACGCGAACGGGTAGAGGGCGGGCTTCTCGGTGACTCCTGC
TACCGCATAGGACGTTTTCC

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MFGQDPPAGWTLDGEERRRWKELGSRYRLYPQLELAERQHLVAETRRWLDRLRRQQEQAQSGPPSPALPPPKGLSLEQPL
QFLKGIGPKSAEKLAQLGLHTVRDALYYFPRDHLDYSRRVLIRQAKVGETVTLVGTVKSCSCFTSPKNPLLTILNLKIAD
KSGIVTLSRFYSGRQFAQRSWQAALERQYPKGAVVAASGLVKKGRLGLTLDNPQLEVLGGEGEDPERLGKILPVYPLSEG
IPADLVRKAVQLALPAAALVEDPLPEPLKRQLQLLDLPLALQQIHFPKTQELLAQARRRLVFDEFFYLQLSLLQRRQRYR
AQAQAVPRYQPARGELLDRFYQILPFQLTAGQQRAIDEILADLRDPLPMNRLLQGDVGSGKTVVAVAALLAAIQSGWQAA
LMAPTEVLAEQHYRKLVEWLSQLQVPVELLTGSTPPAKRREILRQLQTGELPLVVGTHALIQPAVQFRNLGLVVIDEQHR
FGVEQRAALQQKGDHPDVLTMTATPIPRTLTLALHGDLDVTQIDELPPGRKPVHTVVARPGDRLQVVRLMEREIAQGRQA
YVVLPLIEESEKLDLKSAIEEHQRLQEKVFPQFRVGLLHGRLTSSEKEAVIEAFRRRELDILVSTTVVEVGVDVPNASVM
LVEHAERFGLSQLHQLRGRVGRGSDQAYCILMTGSQSEEALRRLKVLEQSHDGFFIAEMDLRFRGPGEVMGTRQSGLPDF
ALASLMDDQEVLQLARQAAEQLIAQDPELQQWPRLRRILEERQARLLGPALLT

Sequences:

>Translated_773_residues
MFGQDPPAGWTLDGEERRRWKELGSRYRLYPQLELAERQHLVAETRRWLDRLRRQQEQAQSGPPSPALPPPKGLSLEQPL
QFLKGIGPKSAEKLAQLGLHTVRDALYYFPRDHLDYSRRVLIRQAKVGETVTLVGTVKSCSCFTSPKNPLLTILNLKIAD
KSGIVTLSRFYSGRQFAQRSWQAALERQYPKGAVVAASGLVKKGRLGLTLDNPQLEVLGGEGEDPERLGKILPVYPLSEG
IPADLVRKAVQLALPAAALVEDPLPEPLKRQLQLLDLPLALQQIHFPKTQELLAQARRRLVFDEFFYLQLSLLQRRQRYR
AQAQAVPRYQPARGELLDRFYQILPFQLTAGQQRAIDEILADLRDPLPMNRLLQGDVGSGKTVVAVAALLAAIQSGWQAA
LMAPTEVLAEQHYRKLVEWLSQLQVPVELLTGSTPPAKRREILRQLQTGELPLVVGTHALIQPAVQFRNLGLVVIDEQHR
FGVEQRAALQQKGDHPDVLTMTATPIPRTLTLALHGDLDVTQIDELPPGRKPVHTVVARPGDRLQVVRLMEREIAQGRQA
YVVLPLIEESEKLDLKSAIEEHQRLQEKVFPQFRVGLLHGRLTSSEKEAVIEAFRRRELDILVSTTVVEVGVDVPNASVM
LVEHAERFGLSQLHQLRGRVGRGSDQAYCILMTGSQSEEALRRLKVLEQSHDGFFIAEMDLRFRGPGEVMGTRQSGLPDF
ALASLMDDQEVLQLARQAAEQLIAQDPELQQWPRLRRILEERQARLLGPALLT
>Mature_773_residues
MFGQDPPAGWTLDGEERRRWKELGSRYRLYPQLELAERQHLVAETRRWLDRLRRQQEQAQSGPPSPALPPPKGLSLEQPL
QFLKGIGPKSAEKLAQLGLHTVRDALYYFPRDHLDYSRRVLIRQAKVGETVTLVGTVKSCSCFTSPKNPLLTILNLKIAD
KSGIVTLSRFYSGRQFAQRSWQAALERQYPKGAVVAASGLVKKGRLGLTLDNPQLEVLGGEGEDPERLGKILPVYPLSEG
IPADLVRKAVQLALPAAALVEDPLPEPLKRQLQLLDLPLALQQIHFPKTQELLAQARRRLVFDEFFYLQLSLLQRRQRYR
AQAQAVPRYQPARGELLDRFYQILPFQLTAGQQRAIDEILADLRDPLPMNRLLQGDVGSGKTVVAVAALLAAIQSGWQAA
LMAPTEVLAEQHYRKLVEWLSQLQVPVELLTGSTPPAKRREILRQLQTGELPLVVGTHALIQPAVQFRNLGLVVIDEQHR
FGVEQRAALQQKGDHPDVLTMTATPIPRTLTLALHGDLDVTQIDELPPGRKPVHTVVARPGDRLQVVRLMEREIAQGRQA
YVVLPLIEESEKLDLKSAIEEHQRLQEKVFPQFRVGLLHGRLTSSEKEAVIEAFRRRELDILVSTTVVEVGVDVPNASVM
LVEHAERFGLSQLHQLRGRVGRGSDQAYCILMTGSQSEEALRRLKVLEQSHDGFFIAEMDLRFRGPGEVMGTRQSGLPDF
ALASLMDDQEVLQLARQAAEQLIAQDPELQQWPRLRRILEERQARLLGPALLT

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=697, Percent_Identity=41.6068866571019, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1787357, Length=383, Percent_Identity=37.8590078328982, Blast_Score=268, Evalue=9e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 86909; Mature: 86909

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGQDPPAGWTLDGEERRRWKELGSRYRLYPQLELAERQHLVAETRRWLDRLRRQQEQAQ
CCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGPPSPALPPPKGLSLEQPLQFLKGIGPKSAEKLAQLGLHTVRDALYYFPRDHLDYSRRV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHH
LIRQAKVGETVTLVGTVKSCSCFTSPKNPLLTILNLKIADKSGIVTLSRFYSGRQFAQRS
HHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCEEHHHHHCCHHHHHHH
WQAALERQYPKGAVVAASGLVKKGRLGLTLDNPQLEVLGGEGEDPERLGKILPVYPLSEG
HHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCEEEECCCCCCHHHHCCCCCCCCCCCC
IPADLVRKAVQLALPAAALVEDPLPEPLKRQLQLLDLPLALQQIHFPKTQELLAQARRRL
CCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VFDEFFYLQLSLLQRRQRYRAQAQAVPRYQPARGELLDRFYQILPFQLTAGQQRAIDEIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ADLRDPLPMNRLLQGDVGSGKTVVAVAALLAAIQSGWQAALMAPTEVLAEQHYRKLVEWL
HHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHH
SQLQVPVELLTGSTPPAKRREILRQLQTGELPLVVGTHALIQPAVQFRNLGLVVIDEQHR
HHHCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCEEEEECHHH
FGVEQRAALQQKGDHPDVLTMTATPIPRTLTLALHGDLDVTQIDELPPGRKPVHTVVARP
CCHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHCCCCCCCCHHHHHCCC
GDRLQVVRLMEREIAQGRQAYVVLPLIEESEKLDLKSAIEEHQRLQEKVFPQFRVGLLHG
CCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHC
RLTSSEKEAVIEAFRRRELDILVSTTVVEVGVDVPNASVMLVEHAERFGLSQLHQLRGRV
CCCCCHHHHHHHHHHHCCHHHEEEHHHHEECCCCCCCCEEEEHHHHHCCHHHHHHHHHCC
GRGSDQAYCILMTGSQSEEALRRLKVLEQSHDGFFIAEMDLRFRGPGEVMGTRQSGLPDF
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCHH
ALASLMDDQEVLQLARQAAEQLIAQDPELQQWPRLRRILEERQARLLGPALLT
HHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHCC
>Mature Secondary Structure
MFGQDPPAGWTLDGEERRRWKELGSRYRLYPQLELAERQHLVAETRRWLDRLRRQQEQAQ
CCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGPPSPALPPPKGLSLEQPLQFLKGIGPKSAEKLAQLGLHTVRDALYYFPRDHLDYSRRV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHH
LIRQAKVGETVTLVGTVKSCSCFTSPKNPLLTILNLKIADKSGIVTLSRFYSGRQFAQRS
HHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCEEHHHHHCCHHHHHHH
WQAALERQYPKGAVVAASGLVKKGRLGLTLDNPQLEVLGGEGEDPERLGKILPVYPLSEG
HHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCEEEECCCCCCHHHHCCCCCCCCCCCC
IPADLVRKAVQLALPAAALVEDPLPEPLKRQLQLLDLPLALQQIHFPKTQELLAQARRRL
CCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VFDEFFYLQLSLLQRRQRYRAQAQAVPRYQPARGELLDRFYQILPFQLTAGQQRAIDEIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ADLRDPLPMNRLLQGDVGSGKTVVAVAALLAAIQSGWQAALMAPTEVLAEQHYRKLVEWL
HHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHH
SQLQVPVELLTGSTPPAKRREILRQLQTGELPLVVGTHALIQPAVQFRNLGLVVIDEQHR
HHHCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCEEEEECHHH
FGVEQRAALQQKGDHPDVLTMTATPIPRTLTLALHGDLDVTQIDELPPGRKPVHTVVARP
CCHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHCCCCCCCCHHHHHCCC
GDRLQVVRLMEREIAQGRQAYVVLPLIEESEKLDLKSAIEEHQRLQEKVFPQFRVGLLHG
CCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHC
RLTSSEKEAVIEAFRRRELDILVSTTVVEVGVDVPNASVMLVEHAERFGLSQLHQLRGRV
CCCCCHHHHHHHHHHHCCHHHEEEHHHHEECCCCCCCCEEEEHHHHHCCHHHHHHHHHCC
GRGSDQAYCILMTGSQSEEALRRLKVLEQSHDGFFIAEMDLRFRGPGEVMGTRQSGLPDF
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCHH
ALASLMDDQEVLQLARQAAEQLIAQDPELQQWPRLRRILEERQARLLGPALLT
HHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]