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The map label for this gene is ycjG [H]

Identifier: 86606608

GI number: 86606608

Start: 1973893

End: 1974942

Strand: Reverse

Name: ycjG [H]

Synonym: CYA_1963

Alternate gene names: 86606608

Gene position: 1974942-1973893 (Counterclockwise)

Preceding gene: 86606616

Following gene: 86606607

Centisome position: 67.34

GC content: 62.38

Gene sequence:

>1050_bases
ATGCGGCTCTACGTCGAACCTTTCACCGTTCACAAGCGCTACGCCTTGACCATCAGCCGAGGTACCCACAGCGATACAAC
CTTGGCTTGGGTGCGAATTGAGGAGGAGGGAATTGAAGGGTGGGGAGAGGCTTGCCCTTTTTCGGTGAGCGACGATTGCC
ACCAGTCTCTGGACGACATCCTGATGGGCTTGGACGTGGCCAAAGGGATCCTGCGCCGCCATTCCCCCTGGGAGCGCCAG
CCAATCGAGGCAGAACTGCTGCGGGCCGGGATCCCTTCGGCAGCGCGGGCAGCGGTGGATATGGCCCTGCACGATTGGAT
GGGCAAACGGCTGGGGAGGCCCCTGTGGCAGTTGTGGGGGCTGGATCGCTCCCGCATCCCGCCCACCACTTTGACCATTG
GCATTTCCACCCCTCAGGCGGCCCGCCAGCGTGCCTTGGCCTGGATGGAACAGACCCAAGCGCGGGCCTTCAAGATCAAG
CTGGGCAGCGACTTGGGGCTGATGGCGGATCAGAACATGCTGATTGCTGTACAAGAGGTGATCCCGCTGGGATCCCTGAT
TACAGTGGATGCCAACGGCGCTTGGACGGTGGAGCAAACCCTGAGCATGAGCAATTGGCTGGCGGGGCAGGGGGTGAGCT
ACCTGGAGCAGCCCTTGGAGAGGGGACAGGAGGCGAAGCTGATCACCCTCTGGCACGAGTCGAAGCTGCCCATCTTCGTG
GATGAGAGTTGTTTCGACAGCCGCGATATTCCCGCCCTGGTGGATCGGGTGCACGGCATCAACATCAAGCTGATGAAGTG
CGGCGGCCTTAGCGAAGCCTGGCGCATGATCCACACCGCCAAAGCCTATGGGCTCCAGGTGATGCTGGGCTGCTACAGCA
ACACCGCCCTGGGCAACACGGCGGCGGCCCACCTAGCGCCGCTGGTGGACTACGTGGACTTAGACAGCCACCTCAATCTC
ATCGACGATCCCTTTTCCGGGGCTTCGGTGCAGGAGGGCTGTCTCATCCCCACCGACCGACCCGGCCTAGGGGTGGAGTT
GCGGGAGTAG

Upstream 100 bases:

>100_bases
GGCTTGGGGACGGGGGCAGGCTGGGCTGGGAACCAAGCTAAGCTCAAAGGGACGGCCCAGGCCCAATTGCCCCTCCAAGC
TTCTTCTTCTGGAGATGCCC

Downstream 100 bases:

>100_bases
GACCATGCTCTCCAGTCAAACTCGGGTGGCCATCCTGCTCCACGGCGGCCTGTTGGGGGAGCACGGCAAAACAGGGCTGG
GGCTATTGCGCTATGGGCCT

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGIEGWGEACPFSVSDDCHQSLDDILMGLDVAKGILRRHSPWERQ
PIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWGLDRSRIPPTTLTIGISTPQAARQRALAWMEQTQARAFKIK
LGSDLGLMADQNMLIAVQEVIPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
DESCFDSRDIPALVDRVHGINIKLMKCGGLSEAWRMIHTAKAYGLQVMLGCYSNTALGNTAAAHLAPLVDYVDLDSHLNL
IDDPFSGASVQEGCLIPTDRPGLGVELRE

Sequences:

>Translated_349_residues
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGIEGWGEACPFSVSDDCHQSLDDILMGLDVAKGILRRHSPWERQ
PIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWGLDRSRIPPTTLTIGISTPQAARQRALAWMEQTQARAFKIK
LGSDLGLMADQNMLIAVQEVIPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
DESCFDSRDIPALVDRVHGINIKLMKCGGLSEAWRMIHTAKAYGLQVMLGCYSNTALGNTAAAHLAPLVDYVDLDSHLNL
IDDPFSGASVQEGCLIPTDRPGLGVELRE
>Mature_349_residues
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGIEGWGEACPFSVSDDCHQSLDDILMGLDVAKGILRRHSPWERQ
PIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWGLDRSRIPPTTLTIGISTPQAARQRALAWMEQTQARAFKIK
LGSDLGLMADQNMLIAVQEVIPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
DESCFDSRDIPALVDRVHGINIKLMKCGGLSEAWRMIHTAKAYGLQVMLGCYSNTALGNTAAAHLAPLVDYVDLDSHLNL
IDDPFSGASVQEGCLIPTDRPGLGVELRE

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=318, Percent_Identity=35.2201257861635, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 38613; Mature: 38613

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGIEGWGEACPFSVSDDCHQSLDDI
CEEEECCEEECEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
LMGLDVAKGILRRHSPWERQPIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWG
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHC
LDRSRIPPTTLTIGISTPQAARQRALAWMEQTQARAFKIKLGSDLGLMADQNMLIAVQEV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCEEEEHHH
IPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
CCCCCEEEEECCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEE
DESCFDSRDIPALVDRVHGINIKLMKCGGLSEAWRMIHTAKAYGLQVMLGCYSNTALGNT
CCCCCCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEEECCCCCCCCH
AAAHLAPLVDYVDLDSHLNLIDDPFSGASVQEGCLIPTDRPGLGVELRE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEECC
>Mature Secondary Structure
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGIEGWGEACPFSVSDDCHQSLDDI
CEEEECCEEECEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
LMGLDVAKGILRRHSPWERQPIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWG
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHC
LDRSRIPPTTLTIGISTPQAARQRALAWMEQTQARAFKIKLGSDLGLMADQNMLIAVQEV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCEEEEHHH
IPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
CCCCCEEEEECCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEE
DESCFDSRDIPALVDRVHGINIKLMKCGGLSEAWRMIHTAKAYGLQVMLGCYSNTALGNT
CCCCCCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEEECCCCCCCCH
AAAHLAPLVDYVDLDSHLNLIDDPFSGASVQEGCLIPTDRPGLGVELRE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]