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The map label for this gene is ybbB

Identifier: 86606563

GI number: 86606563

Start: 1927378

End: 1928427

Strand: Reverse

Name: ybbB

Synonym: CYA_1917

Alternate gene names: 86606563

Gene position: 1928427-1927378 (Counterclockwise)

Preceding gene: 86606566

Following gene: 86606561

Centisome position: 65.75

GC content: 61.9

Gene sequence:

>1050_bases
GTGTCTCCTCCGATTCACTTCACTGCAGAGCCCTGGCTGCACACCTACAGCGAAATTATCGATGTGCGCTCTCCGGCAGA
GTTTGCCGAAGACCATTGGCCGGGGGCCATCAACTTGCCCGTGCTCGACGATGCAGAACGGGCAGAGGTGGGCACCCTCT
ACAGGCAAGTGGATCCCTTTACAGCCCGCAAGCGGGGGGCCAGCCTGGTGGCCCAGAACATCGCCCGCCATTTGCAGACC
CACTTTGCCGACAAGGGCCGGGACTACCACCCCCTCATCTACTGTTGGCGGGGCGGGCAGCGCTCGGCCAGTCTGGCGCT
GGTGTTGTCCCAGGTGGGCTGGCAGGTAACCGTCTTGCAAGGGGGGTACAAAACCTACCGCGCCTGGGTGCGCCGACAAC
TGCAGACTTTGCCCCAGGAGTTTACCTACCGCGTCCTCTGCGGGCCAACGGGGAGCGGCAAAACCCAGTTGCTGCGCCAT
CTGCAGCAGCGGGGAGCCCAAGTCCTCGATCTGGAAAAGCTGGCCTGCCACCGCGGATCGGTGCTGGGGGCCGAGTGGGG
GGTGCCACAGCCCAGTCAGAAGAGCTTTGAAAGTCAACTGGTGCAGGCCTTTCTCCAGTGGGATCCCGCCAAGCCGGTCT
GGGTGGAGTCGGAAAGTGCCAAAGTGGGCCAGCTTCATTTGCCCCCTGCTCTTTGGCAGGCCATCACCCAAGCCGACTGC
GTAGAGGTGCAGTTGCCTCTAGAAGAACGGGTGAAGGGGTTGATCGCCCACTATCCCCACTGGATAGCCCACCCCCAGGC
GCTAAAGGAAAAATTGCTGTCTTTGAAGTCCCGCTACGGCCAGGCCTGTTTGCAGCGCTGGTTTGAGCTCATCGACGCCC
AAGACTGGCCGCAATTTGTGCGGGAGCTACTGCAGCAGCACTACGATCCCACCTATCGCCACGGCCTGCAGCAACACTTT
AACCGTATCCGCGCTCGCCTTGAGCTGCCTGACCTCTCCGAGAGCAGCCTACAGGAGGCCGCTGTCCGACTCAGGGGGAT
GTCGTTTTGA

Upstream 100 bases:

>100_bases
GCGCTTGACCTCGGCTCTCCCAAGTTAAAGCTGGGGCGGTGAGCCTTGGCCAGAACCCTGCCAAGATAGAACACAGCTTA
CCATTTCTATCCGCAGGTTT

Downstream 100 bases:

>100_bases
CCTGCGCCAGCCCTCGCCGGGCAAGCGCGACCAAGTCTGGCGGCGGAAATGTCAAGCAAAGTCCTGTTTGCCCCTCTCAG
AGGGGAACTAGGGACTTCGA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLDDAERAEVGTLYRQVDPFTARKRGASLVAQNIARHLQT
HFADKGRDYHPLIYCWRGGQRSASLALVLSQVGWQVTVLQGGYKTYRAWVRRQLQTLPQEFTYRVLCGPTGSGKTQLLRH
LQQRGAQVLDLEKLACHRGSVLGAEWGVPQPSQKSFESQLVQAFLQWDPAKPVWVESESAKVGQLHLPPALWQAITQADC
VEVQLPLEERVKGLIAHYPHWIAHPQALKEKLLSLKSRYGQACLQRWFELIDAQDWPQFVRELLQQHYDPTYRHGLQQHF
NRIRARLELPDLSESSLQEAAVRLRGMSF

Sequences:

>Translated_349_residues
MSPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLDDAERAEVGTLYRQVDPFTARKRGASLVAQNIARHLQT
HFADKGRDYHPLIYCWRGGQRSASLALVLSQVGWQVTVLQGGYKTYRAWVRRQLQTLPQEFTYRVLCGPTGSGKTQLLRH
LQQRGAQVLDLEKLACHRGSVLGAEWGVPQPSQKSFESQLVQAFLQWDPAKPVWVESESAKVGQLHLPPALWQAITQADC
VEVQLPLEERVKGLIAHYPHWIAHPQALKEKLLSLKSRYGQACLQRWFELIDAQDWPQFVRELLQQHYDPTYRHGLQQHF
NRIRARLELPDLSESSLQEAAVRLRGMSF
>Mature_348_residues
SPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLDDAERAEVGTLYRQVDPFTARKRGASLVAQNIARHLQTH
FADKGRDYHPLIYCWRGGQRSASLALVLSQVGWQVTVLQGGYKTYRAWVRRQLQTLPQEFTYRVLCGPTGSGKTQLLRHL
QQRGAQVLDLEKLACHRGSVLGAEWGVPQPSQKSFESQLVQAFLQWDPAKPVWVESESAKVGQLHLPPALWQAITQADCV
EVQLPLEERVKGLIAHYPHWIAHPQALKEKLLSLKSRYGQACLQRWFELIDAQDWPQFVRELLQQHYDPTYRHGLQQHFN
RIRARLELPDLSESSLQEAAVRLRGMSF

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=349, Percent_Identity=33.810888252149, Blast_Score=134, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 39979; Mature: 39848

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLDDAERAEVGTLYRQVDPF
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCCH
TARKRGASLVAQNIARHLQTHFADKGRDYHPLIYCWRGGQRSASLALVLSQVGWQVTVLQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEE
GGYKTYRAWVRRQLQTLPQEFTYRVLCGPTGSGKTQLLRHLQQRGAQVLDLEKLACHRGS
CCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
VLGAEWGVPQPSQKSFESQLVQAFLQWDPAKPVWVESESAKVGQLHLPPALWQAITQADC
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHHHCCC
VEVQLPLEERVKGLIAHYPHWIAHPQALKEKLLSLKSRYGQACLQRWFELIDAQDWPQFV
EEEECCHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RELLQQHYDPTYRHGLQQHFNRIRARLELPDLSESSLQEAAVRLRGMSF
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLDDAERAEVGTLYRQVDPF
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCCH
TARKRGASLVAQNIARHLQTHFADKGRDYHPLIYCWRGGQRSASLALVLSQVGWQVTVLQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEE
GGYKTYRAWVRRQLQTLPQEFTYRVLCGPTGSGKTQLLRHLQQRGAQVLDLEKLACHRGS
CCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
VLGAEWGVPQPSQKSFESQLVQAFLQWDPAKPVWVESESAKVGQLHLPPALWQAITQADC
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHHHCCC
VEVQLPLEERVKGLIAHYPHWIAHPQALKEKLLSLKSRYGQACLQRWFELIDAQDWPQFV
EEEECCHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RELLQQHYDPTYRHGLQQHFNRIRARLELPDLSESSLQEAAVRLRGMSF
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]