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The map label for this gene is nifB

Identifier: 86606479

GI number: 86606479

Start: 1832797

End: 1834233

Strand: Reverse

Name: nifB

Synonym: CYA_1828

Alternate gene names: 86606479

Gene position: 1834233-1832797 (Counterclockwise)

Preceding gene: 86606480

Following gene: 86606478

Centisome position: 62.54

GC content: 62.0

Gene sequence:

>1437_bases
ATGCCGCCCCTTTCAACTCCGCCCCGCCAGGGATCCCCCTTCCCATCGGCCTGCGCTTGCAGCAGCAGCCCCCGCCAGGC
TCTCCCCCCTCATCTGCAGCAACGCATCGCCACCCATCCCTGCTACAGCGAAGCAGCTCACCATCACTACGCCCGCATGC
ACGTGGCGGTAGCCCCCGCCTGCAACATCCAGTGCAACTACTGCAACCGCAAGTTCGACTGTGCCAACGAGAGCCGGCCC
GGTGTGGTCAGCGAGCTGCTGACCCCGGCGGAAGCCGCCCACAAAGTGCTGGTGATTGCCGGCAAGATCCCGCAACTGAC
GGTGGTGGGCATTGCTGGGCCTGGGGATCCCTTGGCCAACCCCAAGCAAACCTTCGAGACCTTTGCCCGCATTGCCGAGA
AAGCTCCGGATCTCAAGCTCTGCCTTTCCACCAACGGCCTGATGTTGCCGGACTACATCGACGAGATCAAACGGCTGAAC
ATCGACCACGTCACCCTGACCATCAACATGGTGGATCCAGAGATCGGGGCCAGGATCTACCCCTGGATCCGCTGGCGGCG
CAAGCGCATTCGGGGGGTGGAGGCAGCCCGCATCCTGCACGAGCGGCAGATGGAAAGCCTGGATCTGTTGCGGGAGGCAG
ACATCCTCTGCAAAGTCAACTCCGTTCTCATTCCCGGCATCAACGACACCCACCTGCTGGAAGTGAACCGGGTGATCCAG
GAGAAAGGGGCTTTCTTGCACAACATCATGCCCCTGATCTCGGCGCCTGAACACGGCACCTACTTCGGCCTTATCGGCCA
GCGGGGCCCCACCCCTCAGGAGTTGAAAGCCCTGCAAGATCGGTGCTCCGGCAACATGCGGATGATGCGCCACTGTCGCC
AGTGTCGGGCGGACGCGGTGGGCCTGCTGGGGGAAGACCGCAGCCAAGAGTTTACCAAGGAGCGCTTTATGCAGATGACC
CCCGAATACGACCCCGAAAAACGCCGCCAGGTCCAGGCAAGCATTGCCCAACTGCAAGCTCAGCAGCAGTCAACCCCAAC
CCACTGGACAGCCGACCTGCCTCCCGACAGCCCCTCCATCCTGGTGGCCGTAGCCAGCAAAGGCGGCGGCCTGGTCAATC
GCCACTTCGGCCATGCGCGGGAGTTTTTGATCTACGAGGTCAACGCCCGTGGGGCGCAGTTTGTCGGCCACCGCAAGGTT
CCGCAGTACTGCCACGGGGGATCCGGGGAGGACAACAACCTAGAGCAAATCCTGGATCTCATCAGGGATTGCCGAGCCGT
GCTGGTCTCCAAGATCGGCGATTGCCCCATGAAGCGTATTCGCGAGGCCGGCATCGAAGTGGTGGAAGACTACGACCTCA
TCGAAACGGTGGCCCTCAAGTTTTACCGCACCTGGTTGGTCAACCAGGCCCTGGACAGCAAATCTTCCCGATCTTGA

Upstream 100 bases:

>100_bases
CAATAAACTCGATCAGTTCTACCGGGCTGAGTGCAACGGAGCGGGCAGAGGGCGGTTGATATAACCGCCAGTAACGTTCT
ATCCCCTGGTTGACCCCACT

Downstream 100 bases:

>100_bases
GACCTCATCTTTTGGAGACGCGGCCATGGCTACTGCAACTGCTCTCACCAAAGGCGGACTGCCCTGGATCCCACAGTTTA
TCGAAAGCCTGGATATCAGC

Product: nitrogenase cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MPPLSTPPRQGSPFPSACACSSSPRQALPPHLQQRIATHPCYSEAAHHHYARMHVAVAPACNIQCNYCNRKFDCANESRP
GVVSELLTPAEAAHKVLVIAGKIPQLTVVGIAGPGDPLANPKQTFETFARIAEKAPDLKLCLSTNGLMLPDYIDEIKRLN
IDHVTLTINMVDPEIGARIYPWIRWRRKRIRGVEAARILHERQMESLDLLREADILCKVNSVLIPGINDTHLLEVNRVIQ
EKGAFLHNIMPLISAPEHGTYFGLIGQRGPTPQELKALQDRCSGNMRMMRHCRQCRADAVGLLGEDRSQEFTKERFMQMT
PEYDPEKRRQVQASIAQLQAQQQSTPTHWTADLPPDSPSILVAVASKGGGLVNRHFGHAREFLIYEVNARGAQFVGHRKV
PQYCHGGSGEDNNLEQILDLIRDCRAVLVSKIGDCPMKRIREAGIEVVEDYDLIETVALKFYRTWLVNQALDSKSSRS

Sequences:

>Translated_478_residues
MPPLSTPPRQGSPFPSACACSSSPRQALPPHLQQRIATHPCYSEAAHHHYARMHVAVAPACNIQCNYCNRKFDCANESRP
GVVSELLTPAEAAHKVLVIAGKIPQLTVVGIAGPGDPLANPKQTFETFARIAEKAPDLKLCLSTNGLMLPDYIDEIKRLN
IDHVTLTINMVDPEIGARIYPWIRWRRKRIRGVEAARILHERQMESLDLLREADILCKVNSVLIPGINDTHLLEVNRVIQ
EKGAFLHNIMPLISAPEHGTYFGLIGQRGPTPQELKALQDRCSGNMRMMRHCRQCRADAVGLLGEDRSQEFTKERFMQMT
PEYDPEKRRQVQASIAQLQAQQQSTPTHWTADLPPDSPSILVAVASKGGGLVNRHFGHAREFLIYEVNARGAQFVGHRKV
PQYCHGGSGEDNNLEQILDLIRDCRAVLVSKIGDCPMKRIREAGIEVVEDYDLIETVALKFYRTWLVNQALDSKSSRS
>Mature_477_residues
PPLSTPPRQGSPFPSACACSSSPRQALPPHLQQRIATHPCYSEAAHHHYARMHVAVAPACNIQCNYCNRKFDCANESRPG
VVSELLTPAEAAHKVLVIAGKIPQLTVVGIAGPGDPLANPKQTFETFARIAEKAPDLKLCLSTNGLMLPDYIDEIKRLNI
DHVTLTINMVDPEIGARIYPWIRWRRKRIRGVEAARILHERQMESLDLLREADILCKVNSVLIPGINDTHLLEVNRVIQE
KGAFLHNIMPLISAPEHGTYFGLIGQRGPTPQELKALQDRCSGNMRMMRHCRQCRADAVGLLGEDRSQEFTKERFMQMTP
EYDPEKRRQVQASIAQLQAQQQSTPTHWTADLPPDSPSILVAVASKGGGLVNRHFGHAREFLIYEVNARGAQFVGHRKVP
QYCHGGSGEDNNLEQILDLIRDCRAVLVSKIGDCPMKRIREAGIEVVEDYDLIETVALKFYRTWLVNQALDSKSSRS

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53437; Mature: 53306

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLSTPPRQGSPFPSACACSSSPRQALPPHLQQRIATHPCYSEAAHHHYARMHVAVAPA
CCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHEEEEECCC
CNIQCNYCNRKFDCANESRPGVVSELLTPAEAAHKVLVIAGKIPQLTVVGIAGPGDPLAN
CCEEECCCCCCCCCCCCCCCCHHHHHCCCHHHCCEEEEEECCCCCEEEEEECCCCCCCCC
PKQTFETFARIAEKAPDLKLCLSTNGLMLPDYIDEIKRLNIDHVTLTINMVDPEIGARIY
HHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHH
PWIRWRRKRIRGVEAARILHERQMESLDLLREADILCKVNSVLIPGINDTHLLEVNRVIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCCCCHHHHHHHHHH
EKGAFLHNIMPLISAPEHGTYFGLIGQRGPTPQELKALQDRCSGNMRMMRHCRQCRADAV
HHCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLLGEDRSQEFTKERFMQMTPEYDPEKRRQVQASIAQLQAQQQSTPTHWTADLPPDSPSI
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCE
LVAVASKGGGLVNRHFGHAREFLIYEVNARGAQFVGHRKVPQYCHGGSGEDNNLEQILDL
EEEEECCCCCCHHHHHCCCEEEEEEEECCCCCHHHCCCCCCHHHCCCCCCCCCHHHHHHH
IRDCRAVLVSKIGDCPMKRIREAGIEVVEDYDLIETVALKFYRTWLVNQALDSKSSRS
HHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PPLSTPPRQGSPFPSACACSSSPRQALPPHLQQRIATHPCYSEAAHHHYARMHVAVAPA
CCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHEEEEECCC
CNIQCNYCNRKFDCANESRPGVVSELLTPAEAAHKVLVIAGKIPQLTVVGIAGPGDPLAN
CCEEECCCCCCCCCCCCCCCCHHHHHCCCHHHCCEEEEEECCCCCEEEEEECCCCCCCCC
PKQTFETFARIAEKAPDLKLCLSTNGLMLPDYIDEIKRLNIDHVTLTINMVDPEIGARIY
HHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHH
PWIRWRRKRIRGVEAARILHERQMESLDLLREADILCKVNSVLIPGINDTHLLEVNRVIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCCCCHHHHHHHHHH
EKGAFLHNIMPLISAPEHGTYFGLIGQRGPTPQELKALQDRCSGNMRMMRHCRQCRADAV
HHCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLLGEDRSQEFTKERFMQMTPEYDPEKRRQVQASIAQLQAQQQSTPTHWTADLPPDSPSI
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCE
LVAVASKGGGLVNRHFGHAREFLIYEVNARGAQFVGHRKVPQYCHGGSGEDNNLEQILDL
EEEEECCCCCCHHHHHCCCEEEEEEEECCCCCHHHCCCCCCHHHCCCCCCCCCHHHHHHH
IRDCRAVLVSKIGDCPMKRIREAGIEVVEDYDLIETVALKFYRTWLVNQALDSKSSRS
HHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]